scholarly journals Chromatin Immunoprecipitation–on-Chip Reveals Stress-Dependent p53 Occupancy in Primary Normal Cells but Not in Established Cell Lines

2008 ◽  
Vol 68 (23) ◽  
pp. 9671-9677 ◽  
Author(s):  
Helena Shaked ◽  
Idit Shiff ◽  
Miriam Kott-Gutkowski ◽  
Zahava Siegfried ◽  
Ygal Haupt ◽  
...  
Keyword(s):  
Cell Lines ◽  
Chromatin Immunoprecipitation ◽  
Normal Cells ◽  
Established Cell Lines ◽  
Established Cell ◽  
On Chip ◽  
Stress Dependent

scholarly journals DNA Methylation May Restrict but Does Not Determine Differential Gene Expression at the Sgy/Tead2 Locus during Mouse Development

2004 ◽  
Vol 24 (5) ◽  
pp. 1968-1982 ◽  
Author(s):  
Kotaro J. Kaneko ◽  
Theo Rein ◽  
Zong-Sheng Guo ◽  
Keith Latham ◽  
Melvin L. DePamphilis
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Differential Gene Expression ◽  
Cell Lines ◽  
Regulatory Sequences ◽  
Mouse Development ◽  
Normal Cells ◽  
Established Cell Lines ◽  
Established Cell ◽  
Differential Gene

ABSTRACT Soggy (Sgy) and Tead2, two closely linked genes with CpG islands, were coordinately expressed in mouse preimplantation embryos and embryonic stem (ES) cells but were differentially expressed in differentiated cells. Analysis of established cell lines revealed that Sgy gene expression could be fully repressed by methylation of the Sgy promoter and that DNA methylation acted synergistically with chromatin deacetylation. Differential gene expression correlated with differential DNA methylation, resulting in sharp transitions from methylated to unmethylated DNA at the open promoter in both normal cells and tissues, as well as in established cell lines. However, neither promoter was methylated in normal cells and tissues even when its transcripts were undetectable. Moreover, the Sgy promoter remained unmethylated as Sgy expression was repressed during ES cell differentiation. Therefore, DNA methylation was not the primary determinant of Sgy/Tead2 expression. Nevertheless, Sgy expression was consistently restricted to basal levels whenever downstream regulatory sequences were methylated, suggesting that DNA methylation restricts but does not regulate differential gene expression during mouse development.

Lysis of human B-lymphocyte-derived lymphoma/leukemia cells of established cell lines by interferon-activated natural killer (NK) cells

1981 ◽  
Vol 28 (4) ◽  
pp. 459-468 ◽  
Author(s):  
Paul K. Pattengale ◽  
Magnus Gidlund ◽  
Kenneth Nilsson ◽  
Christer Sundström ◽  
Anders Örn ◽  
...  
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Cell Lines ◽  
B Lymphocyte ◽  
Leukemia Cells ◽  
Established Cell Lines ◽  
Established Cell

scholarly journals Growth of Nosema cuniculi in established cell lines

1975 ◽  
Vol 9 (1) ◽  
pp. 61-68 ◽  
Author(s):  
T. Waller
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Growth Patterns ◽  
Rabbit Kidney ◽  
Kidney Cells ◽  
Scale Production ◽  
Simple Method ◽  
Established Cell Lines ◽  
Established Cell ◽  
Canine Kidney

Growth patterns of Nosema cuniculi ( Encephalitozoon cuniculi) in cell cultures of bovine kidney, canine kidney, feline lung, and rabbit kidney were studied. All cell cultures used were easy to manage and the last 3 are commercially-available established cell lines. The dog kidney cells were the most suitable for large-scale production of Nosema. When grown in plastic flasks with a bottom area of 75 cm2, the weekly yield from Nosema-infected canine kidney cells during the 10th to 17th week after inoculation was between 4·1 x 107 and 9·9 x 107 spores per flask. An equilibrium was obtained between the Nosema infection and the kidney cells during this time. A simple method for estimating the numbel of harvested spores is also described.

scholarly journals A Virus of the Reoviridae in Established Cell Lines of Drosophila melanogaster

1981 ◽  
Vol 54 (1) ◽  
pp. 23-31 ◽  
Author(s):  
V. E. Alatortsev ◽  
E. V. Ananiev ◽  
E. A. Gushchina ◽  
V. B. Grigoriev ◽  
B. V. Gushchin
Keyword(s):  
Drosophila Melanogaster ◽  
Cell Lines ◽  
Established Cell Lines ◽  
Established Cell

scholarly journals Differential ability of a T-antigen transport-defective mutant of simian virus 40 to transform primary and established rodent cells.

1985 ◽  
Vol 5 (5) ◽  
pp. 1043-1050 ◽  
Author(s):  
R E Lanford ◽  
C Wong ◽  
J S Butel
Keyword(s):  
Cell Lines ◽  
Mouse Embryo ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Wild Type ◽  
Mouse Embryo Fibroblasts ◽  
Established Cell Lines ◽  
Established Cell ◽  
Embryo Fibroblasts

The transforming potential and oncogenicity of a simian virus 40 (SV40) mutant affecting T-antigen (T-ag), SV40(cT)-3, was examined in an effort to dissect T-ag functions in transformation. SV40(cT)-3 has a point mutation at nucleotide 4434 that abolishes the transport of T-ag to the nucleus but does not affect its association with the cell surface. Transfection-transformation assays were performed with primary cells and established cell lines of mouse and rat origin. The efficiency of transformation for established cell lines by SV40(cT)-3 was comparable to that of wild-type SV40, indicating that transformation of established cell lines can occur in the absence of detectable amounts of nuclear T-ag. Transformation of primary mouse embryo fibroblasts by SV40(cT)-3 was markedly influenced by culture conditions; the relative transforming frequency was dramatically reduced in assays involving focus formation in low serum concentrations or anchorage-independent growth. Immunofluorescence tests revealed that the transformed mouse embryo fibroblasts partially transport the mutant cT-ag to the cell nucleus. Transformed cell lines induced by SV40(cT)-3 did not differ in growth properties from wild-type transformants. SV40(cT)-3 was completely defective for the transformation of primary baby rat kidney cells, a primary cell type unable to transport the mutant T-ag to the nucleus. The intracellular localization of cellular protein p53 was found to mimic T-ag distribution in all the transformants analyzed. The mutant virus was weakly oncogenic in vivo: the induction of tumors in newborn hamsters by SV40(cT)-3 was reduced in incidence and delayed in appearance in comparison to wild-type SV40. These observations suggest that cellular transformation is regulated by both nuclear and surface-associated forms of SV40 T-ag.

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