Abstract 587: Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture andin vivo

A study was initiated to test whether the FM1–43 dye technique could beapplied to the study of endocytic membrane activity in two rodent prostatecancer (MAT-LyLu and AT-2) cell lines of markedly different metastaticability. The lipophilic dye FM1–43, which has frequently been used tomonitor endo/exocytic activity in excitable cells was employed. We found,as in excitable tissues, that both strongly metastatic (MAT-LyLu) andweakly metastatic (AT-2) cells in culture take up FM1–43 to give vesicularstaining of a variable pattern, which appeared to differ between the twocell lines. However, unlike excitable tissues, neither cell linesubsequently released the dye. Indeed, both cell lines retained the dyethrough several rounds of cell division suggesting that dye incorporatedby cells does not enter the endo/exocytotic cycle. Uptake of dye wasindependent of temperature, Na+/K+ gradients, pH or metabolism. Wesuggest that passive accumulation of FM1–43 can occur in cancer cells andshould not, automatically, be interpreted as evidence of endocytosis.

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p53 mutant-type in human prostate cancer cells determines the sensitivity to phenethyl isothiocyanate induced growth inhibition

Journal of Experimental & Clinical Cancer Research ◽

10.1186/s13046-019-1267-z ◽

2019 ◽

Vol 38 (1) ◽

Cited By ~ 2

Author(s):

Monika Aggarwal ◽

Rahul Saxena ◽

Nasir Asif ◽

Elizabeth Sinclair ◽

Judy Tan ◽

...

Keyword(s):

Prostate Cancer ◽

Growth Inhibition ◽

Cancer Cells ◽

Human Prostate ◽

Phenethyl Isothiocyanate ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

Mutant Type

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Abstract 3788: Critical role of adapter protein p66shcin phenethyl isothiocyanate-induced apoptosis in human prostate cancer cells

10.1158/1538-7445.am10-3788 ◽

2010 ◽

Author(s):

Dong Xiao ◽

Shivendra V. Singh

Keyword(s):

Prostate Cancer ◽

Cancer Cells ◽

Critical Role ◽

Human Prostate ◽

Adapter Protein ◽

Phenethyl Isothiocyanate ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

Induced Apoptosis

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Glycidamide Promotes the Growth and Migratory Ability of Prostate Cancer Cells by Changing the Protein Expression of Cell Cycle Regulators and Epithelial-to-Mesenchymal Transition (EMT)-Associated Proteins with Prognostic Relevance

International Journal of Molecular Sciences ◽

10.3390/ijms20092199 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2199

Author(s):

Titus Ime Ekanem ◽

Chi-Chen Huang ◽

Ming-Heng Wu ◽

Ding-Yen Lin ◽

Wen-Fu T. Lai ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Cycle ◽

Protein Expression ◽

Cancer Cells ◽

Cancer Patients ◽

Epithelial To Mesenchymal Transition ◽

Gene Signature ◽

Prostate Cancer Cells ◽

Mesenchymal Transition ◽

Prognostic Relevance

Acrylamide (AA) and glycidamide (GA) can be produced in carbohydrate-rich food when heated at a high temperature, which can induce a malignant transformation. It has been demonstrated that GA is more mutagenic than AA. It has been shown that the proliferation rate of some cancer cells are increased by treatment with GA; however, the exact genes that are induced by GA in most cancer cells are not clear. In the present study, we demonstrated that GA promotes the growth of prostate cancer cells through induced protein expression of the cell cycle regulator. In addition, we also found that GA promoted the migratory ability of prostate cancer cells through induced epithelial-to-mesenchymal transition (EMT)-associated protein expression. In order to understand the potential prognostic relevance of GA-mediated regulators of the cell cycle and EMT, we present a three-gene signature to evaluate the prognosis of prostate cancer patients. Further investigations suggested that the three-gene signature (CDK4, TWIST1 and SNAI2) predicted the chances of survival better than any of the three genes alone for the first time. In conclusion, we suggested that the three-gene signature model can act as marker of GA exposure. Hence, this multi-gene panel may serve as a promising outcome predictor and potential therapeutic target in prostate cancer patients.

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