scholarly journals Abstract 1933: Tissue print Vmemimaging: Visualizing bioelectric signatures in cancer

2019 ◽  
Author(s):  
Dany S. Adams ◽  
Brian H. Tracey ◽  
Larry Takiff ◽  
James Kearns ◽  
Stephen P. Naber ◽  
...  
Keyword(s):  
Tissue Print

scholarly journals First Report of Cucurbit aphid-borne yellows virus in Spain

Plant Disease ◽  
2004 ◽  
Vol 88 (8) ◽  
pp. 907-907 ◽  
Author(s):  
M. Juarez ◽  
V. Truniger ◽  
M. A. Aranda
Keyword(s):  
Nucleotide Sequence ◽  
Nucleotide Sequence Identity ◽  
Rt Pcr ◽  
Sequence Comparisons ◽  
E Coli ◽  
Sequence Identity ◽  
Tissue Print ◽  
Sigma Chemical ◽  
Beet Pseudo Yellows Virus ◽  
Cucumis Melo L

In late spring 2003, field-grown melon plants (Cucumis melo L.) showing bright yellowing of older leaves were observed near Valladolises in Campo de Cartagena, Murcia, Spain. Symptoms resembled those caused by viruses of the genus Crinivirus (family Closteroviridae), but absence or very low populations of whiteflies were observed. However, diseased foci showed clear indications of heavy aphid infestations. Later, during the fall of 2003, squash plants (Cucurbita pepo L.) grown in open fields in the same area showed similar symptoms. Tissue print hybridizations to detect Cucurbit yellow stunting disorder virus (CYSDV) and Beet pseudo yellows virus (BPYV) in symptomatic samples were negative. CYSDV and BPYV are two yellowing-inducing criniviruses previously described in Spain. In contrast, standard double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) with antiserum against Cucurbit aphid-borne yellows virus (CABYV; genus Polerovirus, family Luteoviridae) that was kindly provided by H. Lecoq (INRA-Montfavet Cedex, France) were consistently positive. Definitive confirmation of CABYV associated with symptomatic samples was obtained by performing reverse-transcription polymerase chain reaction (RT-PCR) analyses for the CABYV coat protein gene. Total RNA extracts (TRI reagent; Sigma Chemical, St. Louis, MO) were obtained from symptomatic and asymptomatic leaf samples and RT-PCR reactions were carried out using the primers 5′-GAATACGGTCGCGGCTAGAAATC-3′ (CE9) and 5′-CTATTTCGGGTTCTGGACCTGGC-3′ (CE10) based on the CABYV sequence published by Guilley et al. (2). A single DNA product of approximately 600 bp was obtained only from symptomatic samples. Amplified DNA fragments from two independent samples (samples 36-2 and 37-5) were cloned in E. coli and sequenced (GenBank Accession Nos. AY529653 and AY529654). Sequence comparisons showed a 95% nucleotide sequence identity between the two sequences. A 97% and 94% nucleotide sequence identity was found among 36-2 and 37-5, respectively and the CABYV sequence published by Guilley et al. (2). CABYV seems to be widespread throughout the Mediterranean Basin (1,3) but to our knowledge, it has not previously been described in Spain. Additionally, our data suggest that significant genetic variability might be present in the Spanish CABYV populations. References: (1) Y. Abou-Jawdah et al. Crop Prot. 19:217, 2000. (2) H. Guilley et al. Virology 202:1012, 1994. (3) H. Lecoq et al. Plant Pathol. 41:749, 1992.

Three Highly Sensitive and High-Throughput Serological Approaches for Detecting Dickeya dadantii in Sweet Potato

Plant Disease ◽  
2021 ◽  
Vol 105 (4) ◽  
pp. 832-839
Author(s):  
Wanqin He ◽  
Deqing Huang ◽  
Jiayu Wu ◽  
Xue Li ◽  
Yajuan Qian ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Cell Lines ◽  
Immunosorbent Assay ◽  
Root Rot ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dickeya Dadantii ◽  
Crude Extracts ◽  
Tissue Print ◽  
Hybridoma Cell Lines ◽  
Dot Elisa

Sweet potato stem and root rot is an important bacterial disease and often causes serious economic losses to sweet potato. Development of rapid and sensitive detection methods is crucial for diagnosis and management of this disease in field. Here, we report the production of four hybridoma cell lines (25C4, 16C10, 9B1, and 9H10) using Dickeya dadantii strain FY1710 as an immunogen. Monoclonal antibodies (MAbs) produced by these four hybridoma cell lines were highly specific and sensitive for D. dadantii detection. Indirect enzyme-linked immunosorbent assay (indirect-ELISA) results showed that the four MAbs 25C4, 16C10, 9B1, and 9H10 could detect D. dadantii in suspensions diluted to 4.89 × 104, 4.89 × 104, 9.78 × 104, and 9.78 × 104 CFU/ml, respectively. Furthermore, all four MAbs can react strongly and specifically with all four D. dadantii strains used in this study, not with the other seven tested bacterial strains. Using these four MAbs, three different serological approaches, triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), dot-ELISA, and tissue-print-ELISA, were developed for detection of D. dadantii in crude extracts prepared from field-collected sweet potato plants. Among these three methods, TAS-ELISA and dot-ELISA were used to detect D. dadantii in suspensions diluted up to 1.23 × 104 and 1.17 × 106 CFU/ml, respectively, or in sweet potato crude extracts diluted up to 1:3,840 and 1:1,920 (wt/vol, grams per milliliter), respectively. Surprisingly, both TAS-ELISA and dot-ELISA serological approaches were more sensitive than the conventional PCR. Analyses using field-collected sweet potato samples showed that the newly developed TAS-ELISA, dot-ELISA, or tissue-print-ELISA were reliable in detecting D. dadantii in sweet potato tissues. Thus, the three serological approaches were highly valuable for diagnosis of stem and root rot in sweet potato production.

scholarly journals Detection of ‘ Candidatus Liberibacter asiaticus’ in citrus by concurrent tissue print‐based qPCR and immunoassay

2019 ◽  
Vol 68 (4) ◽  
pp. 796-803 ◽  
Author(s):  
S. M. Fu ◽  
H. W. Liu ◽  
Q. H. Liu ◽  
C. Y. Zhou ◽  
J. S. Hartung
Keyword(s):  
Candidatus Liberibacter Asiaticus ◽  
Tissue Print ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

Detection and tissue distribution of potato spindle tuberviroid in infected tomato plants by tissue print hybridization

1997 ◽  
Vol 142 (7) ◽  
pp. 1289-1296 ◽  
Author(s):  
P. Stark-Lorenzen ◽  
M. -C. Guitton ◽  
R. Werner ◽  
H. -P. Mühlbach
Keyword(s):  
Tissue Distribution ◽  
Tomato Plants ◽  
Tissue Print ◽  
Infected Tomato ◽  
Tissue Print Hybridization

scholarly journals 308 IMMUNOLOCALIZATION AND GENE CLONING OF ZEATIN OXYLOSYLTRANSFERASE

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 474d-474
Author(s):  
Ruth C. Martin ◽  
Machteld C. Mok ◽  
David W.S. Mok
Keyword(s):  
Cdna Libraries ◽  
E Coli ◽  
Baculovirus Expression ◽  
Expression Of Genes ◽  
Immature Seeds ◽  
Tissue Print ◽  
Genes Encoding ◽  
In Vitro Systems ◽  
Transformation Systems

Cytokinins are widely used in tissue culture and transformation systems; however, little is known of their mode of action or the mechanisms regulating their levels in plant tissues. We are studying enzymes responsible for the metabolism of zeatin in immature seeds of Phaseolus. Selective expression of genes encoding such enzymes may regulate the level of active cytokinins during seed development as well as in in vitro systems. A zeatin O-xylosyltransferase, which mediates the formation of O-xylosylzeatin from trans-zeatin and UDP-xylose, has been isolated and monoclonal antibodies specific to the enzyme have been produced. Tissue print analyses demonstrated that the enzyme is primarily localized in the endosperm. ln situ localization and EM studies indicated that the enzyme is present in the cytoplasm and the nucleus. cDNA libraries were constructed from immature seed mRNA and immunopositive clones were selected. The products of these clones are being analyzed in E. coli and baculovirus expression systems.

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