Tissue Print-Immunoblot, an Accurate Method for the Detection ofAcremonium coenophialumin Tall Fescue

1991 ◽  
Vol 81 (7) ◽  
pp. 747 ◽  
Author(s):  
K. D. Gwinn
Author(s):  
S. R. Herd ◽  
P. Chaudhari

Electron diffraction and direct transmission have been used extensively to study the local atomic arrangement in amorphous solids and in particular Ge. Nearest neighbor distances had been calculated from E.D. profiles and the results have been interpreted in terms of the microcrystalline or the random network models. Direct transmission electron microscopy appears the most direct and accurate method to resolve this issue since the spacial resolution of the better instruments are of the order of 3Å. In particular the tilted beam interference method is used regularly to show fringes corresponding to 1.5 to 3Å lattice planes in crystals as resolution tests.


Author(s):  
T. Geipel ◽  
W. Mader ◽  
P. Pirouz

Temperature affects both elastic and inelastic scattering of electrons in a crystal. The Debye-Waller factor, B, describes the influence of temperature on the elastic scattering of electrons, whereas the imaginary part of the (complex) atomic form factor, fc = fr + ifi, describes the influence of temperature on the inelastic scattering of electrons (i.e. absorption). In HRTEM simulations, two possible ways to include absorption are: (i) an approximate method in which absorption is described by a phenomenological constant, μ, i.e. fi; - μfr, with the real part of the atomic form factor, fr, obtained from Hartree-Fock calculations, (ii) a more accurate method in which the absorptive components, fi of the atomic form factor are explicitly calculated. In this contribution, the inclusion of both the Debye-Waller factor and absorption on HRTEM images of a (Oll)-oriented GaAs crystal are presented (using the EMS software.Fig. 1 shows the the amplitudes and phases of the dominant 111 beams as a function of the specimen thickness, t, for the cases when μ = 0 (i.e. no absorption, solid line) and μ = 0.1 (with absorption, dashed line).


2002 ◽  
Vol 41 (01) ◽  
pp. 37-41 ◽  
Author(s):  
S. Shung-Shung ◽  
S. Yu-Chien ◽  
Y. Mei-Due ◽  
W. Hwei-Chung ◽  
A. Kao

Summary Aim: Even with careful observation, the overall false-positive rate of laparotomy remains 10-15% when acute appendicitis was suspected. Therefore, the clinical efficacy of Tc-99m HMPAO labeled leukocyte (TC-WBC) scan for the diagnosis of acute appendicitis in patients presenting with atypical clinical findings is assessed. Patients and Methods: Eighty patients presenting with acute abdominal pain and possible acute appendicitis but atypical findings were included in this study. After intravenous injection of TC-WBC, serial anterior abdominal/pelvic images at 30, 60, 120 and 240 min with 800k counts were obtained with a gamma camera. Any abnormal localization of radioactivity in the right lower quadrant of the abdomen, equal to or greater than bone marrow activity, was considered as a positive scan. Results: 36 out of 49 patients showing positive TC-WBC scans received appendectomy. They all proved to have positive pathological findings. Five positive TC-WBC were not related to acute appendicitis, because of other pathological lesions. Eight patients were not operated and clinical follow-up after one month revealed no acute abdominal condition. Three of 31 patients with negative TC-WBC scans received appendectomy. They also presented positive pathological findings. The remaining 28 patients did not receive operations and revealed no evidence of appendicitis after at least one month of follow-up. The overall sensitivity, specificity, accuracy, positive and negative predictive values for TC-WBC scan to diagnose acute appendicitis were 92, 78, 86, 82, and 90%, respectively. Conclusion: TC-WBC scan provides a rapid and highly accurate method for the diagnosis of acute appendicitis in patients with equivocal clinical examination. It proved useful in reducing the false-positive rate of laparotomy and shortens the time necessary for clinical observation.


2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6


Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation


Author(s):  
B.R. Watkin

AN Aberystwyth selection of tall fescue (Festuca arundinacea Schreb.), known as S170, was sown with certified New Zealand white clover (Trifolium repens) and re' clover (T. pratense) and compared under sheep grazing with other grass/clover pastures at the Grasslands Division Regional Station at Lincoln (Watkin, 1975) .


Author(s):  
R.J.M. Hay ◽  
D.L. Ryan

In a series of trials at Grasslands Gore, over 10 years, the late-flowering tetraploid red clover 'Grassland Pawera' was more productive and persistent than other red clover cultivars. The strong summer growth of Pawera meets the need for heavy-weight lamb feed and high quality forage for conservation in intensive sheep farming systems in Southland. Lenient. infrequent defoliation is necessary to maximise DM production and persistence of Pawera. The most compatible of the grasses evaluated was 'Grasslands Roa' tall fescue. However, 'Grasslands Nui' ryegrass will still be the major grass sown with Pawera owing to its widespread acceptance. In ryegrass mixtures, sowing rates of 5-7 kg/ha of red clover were needed to optimise establishment and subsequent yield. Evidence of oestrogenic activity of Pewera to sheep prompted Grasslands Division to select within Pawera for a low formononetin cultivar. Keywords: red clover, Pawera. Hamua, Turoa. G21. G22, G27. oestrogenic activity, Nui ryegrass, Roa tall fescue, Maru phalaris. Southland, sheep grazing, frequency, intensity, quality. seasonal growth


Author(s):  
J.A. Lancashire ◽  
J.L. Brock

Some characteristics of seed quality, establishment rates, performance in mixtures and response to grazing management of 5 new pasture plants with potential in dryland are described. On a dry hill country site in the Wairarapa, the contribution of the sown grasses established in separate plots with clovers under rotational grazing was 'Grasslands Wana' cocksfoot 65%; 'Grasslands Maru' phalaris 23%; 'Grasslands Matua' prairie grass 22%; and 'Grasslands Roa' tall fescue 13% after 2 years. The other main grass species was resident perennial ryegrass which established from buried seed (ca. 240 plants/m*) and had a major impact on the establishment and growth of the sown grasses. On a seasonally dry Manawatu flat land soil 3 grazing managementsviz. set stocked all year (S); rotational all year (R); and combination (Cl (set stocked from lambing to drafting and rotational for the remainder of the year) were applied to mixtures of the new cultivars (except that 'Grasslands Apanui' cocksfoot replaced Wana) with ryegrass and white clover stocked at 20 sheep/ha. After 3 years the contribution of the new cultivars was negligible under S and ryegrass was dominant. The R pastures became cocksfoot dominant and Matua (in winter) and chicory (in summer) contributed more than in the S system. The C system produced the most evenly balanced species contribution with only Roa remaining at (5%. A sub-trial with cocksfoot cultivars demonstrated that Wana maintained better production and tiller density ~ll,000/m2 ) than Apanui (1000/m' ) under set stocking IS). Although some of the new cultivars will require specialised management procedures to fulfil their potential in dryland, the increasing and widespread use of Matua prairie grass in farming suggests that these techniques can be adopted in commercial agriculture provided good technical information is available in a management package when the cultivar is released. Keywords: Dryland, grazing management, mixtures, Matua prairie grass, Wana cocksfoot, Roa tall fescue, Maru phalaris, Chicory


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