scholarly journals Long-term Persistence of Oral Human Papillomavirus Type 16: The HPV Infection in Men (HIM) Study

2015 ◽  
Vol 8 (3) ◽  
pp. 190-196 ◽  
Author(s):  
Christine M. Pierce Campbell ◽  
Aimée R. Kreimer ◽  
Hui-Yi Lin ◽  
William Fulp ◽  
Michael T. O'Keefe ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Hpv Infection ◽  
Human Papillomavirus Type 16

scholarly journals Analysis of mutations in the E6/E7 oncogenes and L1 gene of human papillomavirus 16 cervical cancer isolates from China

2006 ◽  
Vol 87 (5) ◽  
pp. 1181-1188 ◽  
Author(s):  
Yuping Wu ◽  
Yulong Chen ◽  
Longyu Li ◽  
Guifang Yu ◽  
Ying He ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Cancer Patients ◽  
Hpv Infection ◽  
Typing Method ◽  
Hpv16 E6 ◽  
Human Papillomavirus 16 ◽  
Human Papillomavirus Type 16 ◽  
New Variant

Human papillomavirus type 16 (HPV16) has a number of intratypic variants; each has a different geographical distribution and some are associated with enhanced oncogenic potential. Cervical samples were collected from 223 cervical cancer patients and from 196 age-matched control subjects in China. DNA samples were amplified by using primers specific for the E6, E7 and partial L1 regions. Products were sequenced and analysed. It was found by using a PCR–sequence-based typing method that HPV infection rates in China were 92·8 % in cervical cancer patients and 15·8 % in healthy controls. HPV16 was detected in 70·4 % of cervical cancer patients and in 6·1 % of controls. In HPV16-positive cervical cancers, 23·6 % belonged to the prototype, 65·5 % were of the Asian variant, 5·5 % were of African type 1 and 3·6 % were European variants, whilst only one was a new variant that differed from any variant published so far. Prevalences of HPV16 E6 D25E and E113D variants were 67·3 and 9 %, respectively. In addition to D25E and E113D, the following E6 variations were found in this study: R129K, E89Q, S138C, H78Y, L83V and F69L. The results also showed that the prevalences of three hot spots of E7 nucleotide variation, N29S, S63F and a silent variation, nt T846C, were 70·2 % (33/47), 51·1 % (24/47) and 61·7 % (29/47), respectively. The following L1 variations were found in this study: S377A, K387E, E378D, K382E and T379P. It was also found that the average age of Asian variant-positive cervical cancer patients (42·98±10·43 years) was 7·56 years lower than that of prototype-positive patients (50·54±10·91). It is suggested that the high frequency of HPV16 Asian variants might contribute to the high incidence of cervical cancer in China.

Abstract 551: Human papillomavirus (HPV) infection characteristics in a cohort of men who have sex with men (MSM) during long-term follow-up

2012 ◽  
Author(s):  
Dorothy J. Wiley ◽  
Xiuhong Li ◽  
Hilary K. Hsu ◽  
Stephen Young ◽  
Ross D. Cranston ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Hpv Infection ◽  
Long Term Follow Up ◽  
Sex With Men

scholarly journals High Oral Human Papillomavirus Type 16 Load Predicts Long-term Persistence in Individuals With or at Risk for HIV Infection

2015 ◽  
Vol 212 (10) ◽  
pp. 1588-1591 ◽  
Author(s):  
Daniel C. Beachler ◽  
Yingshi Guo ◽  
Wiehong Xiao ◽  
Robert D. Burk ◽  
Howard Minkoff ◽  
...  
Keyword(s):  
At Risk ◽  
Human Papillomavirus ◽  
Hiv Infection ◽  
Human Papillomavirus Type 16

Long-term persistence of human papillomavirus in the skin of the glans penis of elderly men above 80 years of age

2017 ◽  
Vol 29 (6) ◽  
pp. 552-556 ◽  
Author(s):  
Tadaichi Kitamura ◽  
Motofumi Suzuki ◽  
Yasuhiro Koyama ◽  
Kazuyoshi Shigehara
Keyword(s):  
Human Papillomavirus ◽  
Sexual Intercourse ◽  
Age Groups ◽  
The Elderly ◽  
Hpv Infection ◽  
Glans Penis ◽  
Hpv Prevalence ◽  
The Difference ◽  
High Risk Hpv

Investigations of human papillomavirus (HPV) in the glans penis are scarce, especially with regard to its natural history. To elucidate HPV prevalence among Japanese men attending urological clinics, 798 adult participants were recruited consecutively and stratified into groups by age based on 10-year intervals. The overall HPV prevalence among the participants was 24.8%, with 15.5% positive for high-risk HPV and 9.3% infected with other HPVs. The HPV positivity rate was sustained in those over 80 years of age at nearly the same level as those in the younger age groups. We also determined the age at last sexual intercourse of 15 HPV-positive participants in the 80+ years age group. In addition, six participants positive for HPV were re-examined for HPV and all showed nearly the same HPV types as those identified in their first examinations. The difference between the age at the first test and the age of last intercourse was 8.3 ± 6.2 years. Except for the elderly group who reported sexual intercourse within the previous year, the duration was 10.0 ± 5.1 years. Our data suggest an HPV persistence of at least eight years. Further investigation is necessary to elucidate the long-term persistence of HPV infection in the glans penis.

scholarly journals Human Papillomavirus type 16 L2 recruits both retromer and retriever complexes during retrograde trafficking of the viral genome to the cell nucleus.

2020 ◽  
Author(s):  
David Pim ◽  
Justyna Broniarczyk ◽  
Abida Siddiqa ◽  
Paola Massimi ◽  
Lawrence Banks
Keyword(s):  
Human Papillomavirus ◽  
Viral Genome ◽  
Direct Interaction ◽  
Hpv Infection ◽  
Infection Process ◽  
Sorting Nexins ◽  
Minor Capsid Protein ◽  
Human Papillomavirus Type 16 ◽  
Retromer Complex ◽  
Shed Light

Previous studies have identified an interaction between Human Papillomavirus L2 minor capsid protein and sorting nexins 17 and 27 (SNX17 and SNX27) during virus infection. Further studies show involvement of both retromer and retriever complexes in this process, since knockdown of proteins from either complex impairs infection. In this study, we show that HPV L2 and EdU-labelled pseudovirions colocalize with both retromer and retriever, with components of each complex being bound by L2 during infection. We also show that both sorting nexins may interact with either of the recycling complexes, but that the interaction between SNX17 and HPV16 L2 is not responsible for retriever recruitment during infection, instead being required for retromer recruitment. Further, we show that retriever recruitment most likely involves direct interaction between L2 and the C16orf62 subunit of retriever, in a similar manner to its interaction with the VPS35 subunit of retromer. IMPORTANCE Previous studies identified sorting nexins 17 and 27, as well as the retromer complex, as playing a role in HPV infection. This study shows that the newly-identified retriever complex also plays an important role and begins to shed light on how both sorting nexins contribute to retromer and retriever recruitment during the infection process.

scholarly journals Cloning, Expression, and Purification of the rE2 Protein Human Papillomavirus type 16

2020 ◽  
pp. 1-5
Author(s):  
Saucedo Mendiola María Leticia ◽  
Keyword(s):  
Human Papillomavirus ◽  
Sexually Transmitted Disease ◽  
Reliable Method ◽  
Uterine Cancer ◽  
Transmitted Disease ◽  
Hpv Infection ◽  
Expression And Purification ◽  
Men And Women ◽  
Sexually Transmitted ◽  
Human Papillomavirus Type 16

Human papillomavirus (HPV) infection is the most common sexually transmitted disease, both in men and women, and the persistence of this infection is the main causative agent of cervical-uterine cancer. HPV 16 protein E2 is important in viral replication and transcription. For this reason, it is of utmost importance to have a reliable method of expression and purification of recombinant E2 (rE2) proteins to carry out experiments focused on the development of drugs against HPV.

Human Papillomavirus: Epidemiology and Public Health

2003 ◽  
Vol 127 (8) ◽  
pp. 930-934 ◽  
Author(s):  
Mark Schiffman ◽  
Philip E. Castle
Keyword(s):  
Public Health ◽  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Sexually Transmitted Infection ◽  
Viral Persistence ◽  
Hpv Infection ◽  
Transmitted Infection ◽  
Hpv Dna ◽  
Sexually Transmitted

Abstract Approximately 15 types of human papillomavirus (HPV) infection cause virtually all cases of cervical cancer. Human papillomavirus 16 is the major type, accounting for approximately 50% of cases. The major steps of cervical carcinogenesis include HPV infection, viral persistence and progression to precancer (as opposed to viral clearance), and invasion. Human papillomavirus is the most common sexually transmitted infection. However, most HPV infections become undetectable by even sensitive HPV DNA testing within 1 to 2 years. The prevalence of infection peaks at young ages and declines thereafter, perhaps as the result of HPV type-specific acquired immunity. Most HPV infections are neither microscopically evident nor visible, making HPV DNA detection the diagnostic reference standard. Poorly defined immunologic factors are the major determinants of viral outcome. Smoking, multiparity, and long-term oral contraceptive use increase the risk of persistence and progression. Other sexually transmitted infections (eg, Chlamydia trachomatis), chronic inflammation, and nutritional factors might also play a role. Overt, long-term viral persistence in the absence of precancer is uncommon. New prevention strategies can be derived from the evolving knowledge of HPV carcinogenesis. Human papillomavirus vaccination is the ultimate prevention strategy, and large-scale trials are already underway. In the meantime, HPV DNA diagnostics are more sensitive although less specific than cytology, permitting a consideration of lengthened screening intervals. In terms of public health education, clinicians and patients will need to shift discussions of the mildly abnormal Papanicolaou test to consideration of HPV infection as a common sexually transmitted infection that rarely causes cervical cancer.

scholarly journals Structural Analysis Reveals an Amyloid Form of the Human Papillomavirus Type 16 E1∧E4 Protein and Provides a Molecular Basis for Its Accumulation

2008 ◽  
Vol 82 (16) ◽  
pp. 8196-8203 ◽  
Author(s):  
Pauline B. McIntosh ◽  
Stephen R. Martin ◽  
Deborah J. Jackson ◽  
Jameela Khan ◽  
Erin R. Isaacson ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Structural Analysis ◽  
Epithelial Cells ◽  
Molecular Basis ◽  
Hpv Infection ◽  
Fibril Formation ◽  
Amyloid Imaging ◽  
Biopsy Material ◽  
Human Papillomavirus Type 16 ◽  
Imaging Probes

ABSTRACT The abundant human papillomavirus (HPV) type 16 E4 protein exists as two distinct structural forms in differentiating epithelial cells. Monomeric full-length 16E1∧E4 contains a limited tertiary fold constrained by the N and C termini. N-terminal deletions facilitate the assembly of E1∧E4 into amyloid-like fibrils, which bind to thioflavin T. The C-terminal region is highly amyloidogenic, and its deletion abolishes amyloid staining and prevents E1∧E4 accumulation. Amyloid-imaging probes can detect 16E1∧E4 in biopsy material, as well as 18E1∧E4 and 33E1∧E4 in monolayer cells, indicating structural conservation. Our results suggest a role for fibril formation in facilitating the accumulation of E1∧E4 during HPV infection.

scholarly journals Combined Use of Vaccination and Immunotherapy Drugs to Achieve a Long-Term Clinical Remission in Patients with Chronic HPV-infection Manifested in condyloma accuminata of the anogenital area

2016 ◽  
Vol 15 (3) ◽  
pp. 60-66 ◽  
Author(s):  
A. D. Protasov ◽  
Yu. V. Tezikov ◽  
M. P. Kostinov ◽  
I. S. Lipatov ◽  
O. O. Magarshak ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Clinical Remission ◽  
Concomitant Administration ◽  
Hpv Infection ◽  
Recombinant Vaccine ◽  
Complete Disappearance ◽  
Study Objective ◽  
Anogenital Area ◽  
One Year

Study objective. The study aims at evaluating the efficacy of combined administration of imiquimod 5% cr me and human papillomavirus (HPV) quadrivalent recombinant vaccine in order to achieve a long-term clinical remission in patients with chronic HPV infection manifested in condyloma accuminata of the anogenital area. Materials and Methods. The study enrolled 36 subjects aged 26.4 (4.1) years (including 22 men) with 1 to 5 condyloma accuminata of the anogenital area. Study participants were vaccinated with human papillomavirus quadrivalent recombinant vaccine using a 0 - 2 - 6 month regimen with concomitant administration of imiquimod 5% cr me applied 3 times per week for not more than 16 weeks. Results. Complete disappearance of condyloma accuminata was observed in 34 out of 36 subjects (94.4%) after one year from the start of treatment. Two patients still having condyloma accuminata of the anogenital area after one year of combination treatment underwent a successful course of treatment with solcoderm (one patient for 1 year 3 months and the other for 1 year 4 months) that resulted in complete disappearance of condyloma accuminata. Within the 1 year 6 month period no recurrence of condyloma accuminata of the anogenital area has been observed. Conclusion. Vaccination with human papillomavirus quadrivalent recombinant vaccine along with concomitant use of imiquimod 5% cr me enables to achieve a long-term clinical remission in patients with chronic HPV infection manifesting in condyloma accuminata of the anogenital area, in at least 94.4% of patients followed up for 1 year 6 months.

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