Partial Reversal of Tissue Calcification and Extension of Life Span following Ammonium Nitrate Treatment of Klotho-Deficient Mice

SUMMARYTwo 1-year experiments are described in which a single dressing of 120 lb N/acre (134 kg/ha) was applied to a grass sward as anhydrous ammonia (82% N), ammonium nitrate (34·5% N) or sulphate of ammonia (21% N) at various dates from December to April. The pattern of primary growth from each of the treatments was recorded from early April until mid-June by frequent sampling of the herbage to determine the dry matter and crude protein yields. At every date of fertilizer application from late January to mid-April the growth rate of the herbage was markedly lower with anhydrous ammonia than with ammonium nitrate. With the December applications the spring growth rate of herbage from the anhydrous ammonia treatment was similar to that from the ammonium nitrate treatment. Applications of sulphate of ammonia gave results almost identical with those obtained from ammonium nitrate. The calculation of the dates on which a yield of 2000 lb dry matter/acre (2242 kg/ha) would have been available for dairy cow grazing showed clearly a delay of 12–15 days where anhydrous ammonia rather than ammonium nitrate was applied. The response to anhydrous ammonia was improved by applying it early in the year but solid nitrogen fertilizers, applied either early in the season or when growth was starting, gave higher yields of herbage than anhydrous ammonia.

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Systemic human minidystrophin gene transfer improves functions and life span of dystrophin and dystrophin/utrophin-deficient mice

Journal of Orthopaedic Research® ◽

10.1002/jor.20781 ◽

2008 ◽

Vol 27 (4) ◽

pp. 421-426 ◽

Cited By ~ 50

Author(s):

Bing Wang ◽

Juan Li ◽

Freddie H. Fu ◽

Xiao Xiao

Keyword(s):

Gene Transfer ◽

Life Span ◽

Deficient Mice

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The impact of nitrogen fertilizer injection on kernel yield and yield formation of maize

Plant Soil and Environment ◽

10.17221/208/2013-pse ◽

2014 ◽

Vol 60 (No. 1) ◽

pp. 1-7

Author(s):

K. Kubešová ◽

J. Balík ◽

O. Sedlář ◽

L. Peklová

Keyword(s):

Ammonium Nitrate ◽

Field Experiments ◽

Kernel Weight ◽

Climatic Conditions ◽

Calcium Ammonium Nitrate ◽

Nitrate Treatment ◽

Significant Difference ◽

Urea Ammonium Nitrate ◽

Yield Formation ◽

The Impact

In field experiments over three vegetation periods (2010–2012) we studied impact of the CULTAN (controlled uptake long term ammonium nutrition) method on yield and yield parameters of kernel maize. The field experiments were conducted at three sites with different soil-climatic conditions. CULTAN treatments were fertilized once with the total amount of nitrogen using an injection machine (at the canopy height of 20 cm) and compared to conventional fertilization with calcium ammonium nitrate application at pre-sowing preparations. In all treatments the amount of nitrogen was the same, 140 kg N/ha. In 2010 at Humpolec site, CULTAN urea ammonium nitrate + inhibitor of nitrification treatment gave by 20.5% higher number of ears compared to CULTAN urea ammonium nitrate treatment. In 2011 at Ivanovice all CULTAN treatments reached statistically significantly higher number of kernels per ear. The higher 1000 kernel weight at CULTAN treatments was observed in 2012 at the Ivanovice site; a statistically significant difference between conventional and CULTAN urea ammonium nitrate + inhibitor of nitrification treatment was observed. Fertilization of maize with nitrogen using the CULTAN method under the conditions of the Czech Republic provides the same yield certainty as the conventional surface application and the CULTAN method of fertilization increases the yield certainty at delayed sowing. Harvest index was statistically significantly influenced by year, fertilization treatment and site.

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Weed Control in Cole Crops and Onion (Allium cepa) Using Ammonium Nitrate

Weed Science ◽

10.1017/s0043174500094984 ◽

1996 ◽

Vol 44 (4) ◽

pp. 952-958 ◽

Cited By ~ 3

Author(s):

Iris Bitterlich ◽

Mahesh K. Upadhyaya ◽

Solomon I. Shibairo

Keyword(s):

Ammonium Nitrate ◽

Weed Control ◽

Crop Yield ◽

Nitrate Solution ◽

Initial Density ◽

Brussels Sprouts ◽

Nitrate Treatment ◽

Cole Crops ◽

Ammonium Nitrate Solution ◽

Common Purslane

Weed control in cole crops and onion by postemergence spraying with liquid ammonium nitrate was studied to determine its feasibility under Fraser Valley, British Columbia conditions. Ammonium nitrate solutions (7.5, 10, 15 and 20% N) were applied at 800 L ha−1 on warm, sunny days. Shepherd's-purse, low cudweed, redroot pigweed and ladysthumb were susceptible to this treatment; common lambsquarters, common purslane and annual bluegrass were tolerant. In order to determine the effect of weed density on weed control, several shepherd's-purse densities were established in broccoli and sprayed with ammonium nitrate solution (20% N; 800 L ha−1). Two days after treatment, weed control was 87.2% for an initial density of 500 shepherd's-purse plants m−2 (1987) and 76.1% for an initial density of 988 plants m−2 (1988). The seedlings that survived the treatment were sufficiently set back that they did not cause any adverse effect on crop yield. In a separate study, cabbage and onion were found to be tolerant to the ammonium nitrate treatment. Initially, cauliflower, broccoli and Brussels sprouts growth was slightly inhibited by the ammonium nitrate treatment but the plants recovered and crop yield was not affected.

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INFLUENCE OF SOIL MOISTURE TENSION AND AMENDMENTS ON YIELD, OIL, AND PROTEIN CONTENT OF TARGET RAPE GROWN ON GRAY WOODED SOILS IN THE GREENHOUSE

Canadian Journal of Soil Science ◽

10.4141/cjss73-011 ◽

1973 ◽

Vol 53 (1) ◽

pp. 87-93 ◽

Cited By ~ 3

Author(s):

W. F. NUTTALL

Keyword(s):

Soil Moisture ◽

Ammonium Nitrate ◽

Protein Content ◽

Oil Content ◽

Fiber Content ◽

Brassica Napus L ◽

Available Nitrogen ◽

Nitrate Treatment ◽

High Soil Moisture ◽

Loam Soil

Soil moisture tension (100 and 151 mbars) and amendments (straw, peat, manure, and ammonium nitrate) on three Gray Wooded and one Dark Gray soil significantly affected yield, oil, and protein content of Target rapeseed (Brassica napus L.). Low soil moisture tension (LSMT) gave higher yields than high soil moisture tension (HSMT). In general, lower oil content and higher protein content were obtained under HSMT than under LSMT. Also, the ammonium nitrate treatment increased protein content of the grain more than the other amendments. Protein content ranged from 28.2 to 43.3% and oil content from 38.9 to 50.0%. Regression analysis revealed that protein and oil content were inversely related (R2 = 79.6%). Lowest fiber content (highest protein plus oil) was obtained with rape grown on Dark Gray, Nipawin loam soil. Ammonium nitrate (70 μg N/g soil) produced the lowest fiber content averaged over moisture tension and soil type treatments. Those treatments that helped to increase the available nitrogen supply to the crop and that increased protein contents of the grain produced the lowest fiber contents.

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Lysosomal acid lipase-deficient mice: depletion of white and brown fat, severe hepatosplenomegaly, and shortened life span

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)31157-3 ◽

2001 ◽

Vol 42 (4) ◽

pp. 489-500

Author(s):

Hong Du ◽

Martin Heur ◽

Ming Duanmu ◽

Gregory A. Grabowski ◽

David Y. Hui ◽

...

Keyword(s):

Life Span ◽

Brown Fat ◽

Acid Lipase ◽

Lysosomal Acid ◽

Lysosomal Acid Lipase ◽

Deficient Mice

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Microvasculopathy And Soft Tissue Calcification In Mice Are Governed by Fetuin-A, Pyrophosphate And Magnesium

10.1101/577239 ◽

2019 ◽

Cited By ~ 3

Author(s):

Anne Babler ◽

Carlo Schmitz ◽

Andrea Büscher ◽

Marietta Herrmann ◽

Felix Gremse ◽

...

Keyword(s):

Soft Tissue ◽

Molecular Genetic ◽

Extracellular Fluid ◽

Molecular Genetic Analysis ◽

Ectopic Calcification ◽

Special Importance ◽

Soft Tissue Calcification ◽

Fetuin A ◽

Tissue Calcification ◽

Deficient Mice

AbstractObjectiveCalcifications can disrupt organ function in the cardiovascular system and the kidney, and are particularly common in patients with chronic kidney disease (CKD). Fetuin-A deficient mice maintained against the genetic background DBA/2 exhibit particularly severe soft tissue calcifications, while fetuin-A deficient C57BL/6 mice remain healthy. We employed molecular genetic analysis to identify risk factors of calcification in fetuin-A deficient mice. We sought to identify pharmaceutical therapeutic target that could be influenced by dietary of parenteral supplementation.Approach and ResultsWe studied the progeny of an intercross of fetuin-A deficient DBA/2 and C57BL/6 mice to identify candidate risk genes involved in calcification. We determined that a hypomorphic mutation of the Abcc6 gene, a liver ATP transporter supplying systemic pyrophosphate, and failure to regulate the TRPM6 magnesium transporter in kidney were associated with severity of calcification. Calcification prone fetuin-A deficient mice were alternatively treated with dietary phosphate restriction, magnesium supplementation, or by parenteral administration of fetuin-A or pyrophosphate. All treatments markedly reduced soft tissue calcification, demonstrated by computed tomography, histology and tissue calcium measurement.ConclusionsWe show that pathological ectopic calcification in fetuin-A deficient DBA/2 mice is caused by a compound deficiency of three major extracellular and systemic inhibitors of calcification, namely fetuin-A, pyrophosphate, and magnesium. All three of these are individually known to contribute to stabilize protein-mineral complexes and thus inhibit mineral precipitation from extracellular fluid. We show for the first time a compound triple deficiency that can be treated by simple dietary or parenteral supplementation. This is of special importance in patients with advanced CKD, who commonly exhibit reduced serum fetuin-A, pyrophosphate and magnesium levels.Subject CodesAnimal Models of Human Disease, Fibrosis, Inflammation, Proteomics, Peripheral Vascular Disease

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Using Different Types of Fertilization for Increasing Sugar Beet Growth under Sandy Soil Conditions.

Journal of Plant Genetics and Crop Research ◽

10.14302/issn.2641-9467.jgrc-18-1936 ◽

2018 ◽

Vol 1 (1) ◽

pp. 19-39

Author(s):

Zaki, MS ◽

Eman I. El-Sarag ◽

Howaida A. Maamoun ◽

M. H. Mubarak

Keyword(s):

Growth Rate ◽

Sugar Beet ◽

Ammonium Nitrate ◽

Leaf Area ◽

Soil Conditions ◽

Area Index ◽

Urea Treatment ◽

Nitrate Treatment ◽

Leaf Area Duration ◽

Net Assimilation

Four nitrogen forms and four biofertilizer were application as well as their interactions on growth analysis of sugar beet (Beta vulgaris L). The important results could be summarized as follow. Urea treatment achieved maximum increase in Leaf Area Index were 69.71, 81.32 and 166.54 at 120, 140 and 160 day in the 1st season, respectively, The highest one was 160.6 in the 2nd also was ammonium nitrate application at 160 days. Urea treatment inclusion in seeds with ntrobin application resulted the highest values of leaf area index (LAI), crop growth rate (CGR) and leaf area duration (LAD) and in the 1st season. A slight increase was 0.03 g/week in this case was found due to urea treatments as compared with the others treatment at the period from Relative growth rate (RGR3) in the 1st season. Ammonium nitrate treatment achieved the maximum values from Crop Growth Rate was 39.16 g/day in (CGR1), 93.24 and 13.5 g/day in (CGR2) and (CGR3) from urea treatment at the 1st season. The highest net assimilation rate was 0.66 g/dm.week achieved by ntrobin as compared the others treatment whereas, the lowest one 0.11 g.dm /week with the phosphorine application. Ammonium sulphate treatment with (phosphorin + ntrobin) obtained the highest net assimilation rate (NAR) in the 1st season. The highest values from leaf area duration were 0.11, 0.19 and 0.15 dm2/week achieved with urea and ntrobin in the 1st season at (LAD2), (LAD3) and (LAD4). Ammonium nitrate treatment with phosphorin obtained the highest leaf area duration (LAD) in the 2nd season. Generally, it could be recommended that fertilizing sugar beet plants variety Ymer with nitrogen forms inoculated with biofertilizer (ntrobin 600gm/fed) increased the growth of sugar beet plants under sandy soil conditions.

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