Characteristic Cytological Findings of Lobular Endocervical Glandular Hyperplasia Associated with Adenocarcinoma of the Uterine Cervix

2020 ◽  
Vol 64 (6) ◽  
pp. 556-562
Author(s):  
Shuhei Ishii ◽  
Takahiko Ito ◽  
Marisa Yamada ◽  
Naoko Yamazaki ◽  
Koichi Ikebata ◽  
...  
Keyword(s):  
Uterine Cervix ◽  
Mixed Type ◽  
Periodic Acid ◽  
Glandular Cell ◽  
Ki 67 ◽  
Cell Clusters ◽  
Pure Type ◽  
Gastric Type ◽  
Glandular Structures ◽  
Mixed Types

<b><i>Objective:</i></b> To investigate the cytological findings of lobular endocervical glandular hyperplasia (LEGH) associated with adenocarcinoma and to clarify its characteristics and the coexisting adenocarcinoma using histochemistry and immunohistochemistry. <b><i>Methods:</i></b> Eighteen surgical cases of LEGH of the uterine cervix were retrospectively reviewed and classified into 3 groups: pure (pure type), atypical (atypical type), and LEGH with adenocarcinoma (mixed type). The mixed type is defined as LEGH or atypical LEGH with in situ or invasive adenocarcinoma. Cytological findings of conventional endocervical smear specimens (Papanicolaou stain) were analyzed. Histochemistry (periodic acid-Schiff reaction) and immunohistochemistry (M-GGMC-1, Muc-6 glycoprotein, and Ki-67) were performed using tissue specimens. <b><i>Results:</i></b> Cytologically, the pure type (7 cases) is characterized by glandular cell clusters that tended to form monolayered sheets with uniformly small nuclei and contain golden-yellowish mucin, whereas atypical (5 cases) and mixed (6 cases) types are characterized by glandular cell clusters similar to those of the pure type, but with complex glandular structures and mucin localization on the surface of glandular cell clusters. Ki-67 labeling index was significantly higher in atypical and mixed types than that in the pure type. Gastric-type mucinous carcinoma (MC-G) was observed in 2 out of 6 cases with mixed type. <b><i>Conclusions:</i></b> LEGH is found to be associated with adenocarcinoma types other than MC-G. Complex glandular structures or mucin localization on the surface of glandular cell clusters may be useful cytological findings to detect atypical and mixed types of LEGH.

Conjunctival myxoid stromal tumour: a distinctive clinicopathological and immunohistochemical study

2018 ◽  
Vol 103 (9) ◽  
pp. 1259-1265 ◽  
Author(s):  
Xiao-Yi Qin ◽  
Zhe-Hao Jin ◽  
You-Pei Wang ◽  
Zong-Duan Zhang
Keyword(s):  
Smooth Muscle Actin ◽  
Periodic Acid ◽  
Clinical Findings ◽  
Stromal Tumour ◽  
Bulbar Conjunctiva ◽  
Low Grade ◽  
Ki 67 ◽  
Periodic Acid Schiff ◽  
Multiple Spindle ◽  
Immunohistochemical Stains

Background/aimsTo describe the clinicopathological and immunohistochemical characteristics of 10 patients representing a new entity of benign conjunctival myxoid stromal tumours.MethodsRetrospective review of clinical findings, histopathological and immunohistochemical studies identified 10 cases of low-grade conjunctival myxoid stromal tumours. Specimens were routinely processed and stained with H&E. Immunohistochemical stains for CD34, CD68, vimentin, S100, smooth muscle actin (SMA), myosin, desmin, actin, Bcl-2 and Ki-67 were performed. Specific stains for Alcian-blue periodic acid-Schiff (AB-PAS) and aldehyde fuchsin stains were also performed.ResultsTen patients with an average age of 45.6±11.1 years had a tender white or faint yellow to red mass on the bulbar conjunctiva. All the lesions were completely removed, and none of the patients relapsed. Histologically, all neoplasms consisted of spindle-shaped cells that showed signs of pseudonuclear inclusions, multinuclear cells and had no atypia. The stroma consisted of a large amount of mucus and was infiltrated with delicate to ropey collagens, a few mast cells and new vessels. Immunohistochemical stains were positive for CD34, vimentin and Bcl-2; partial positive for CD68; very low for Ki-67; and negative for S100, SMA, myosin, desmin and actin. AB-PAS suggested that the stroma was mucinous.ConclusionsThese rare benign mesenchymal conjunctival tumours are mostly unilateral and occur in the bulbar conjunctiva. Complete resection is the radical treatment. These lesions are characterised by multiple spindle cells, a large amount of mucus, and sharing similar basic histopathological features with conjunctival myxoma and conjunctival stromal tumour. We suggest naming these lesions ‘conjunctival myxoid stromal tumours’.

Expression of Cytokeratins 7 and 20 in Helicobacter pylori-associated Chronic Gastritis in Children

2004 ◽  
Vol 7 (2) ◽  
pp. 180-186 ◽  
Author(s):  
M. Cohen ◽  
E. Cueto Rúa ◽  
N. Balcarce ◽  
R. Drut
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Structural Changes ◽  
Periodic Acid ◽  
Gastric Epithelium ◽  
Surface Epithelium ◽  
Ki 67 ◽  
Periodic Acid Schiff ◽  
Group A ◽  
H Pylori

Helicobacter pylori gastric infection induces structural changes in the gastric epithelium. Among them, variations in the expression of cytokeratins have been reported in adult patients. In the present study, we describe the expression of CK7 and CK20 in gastric samples taken from the antrum in three groups of pediatric patients: (A) Helicobacter pylori-associated chronic gastritis (mean age: 11.4 years); (B) previous H. pylori chronic gastritis patients (mean age: 9.4 years); and (C) controls (mean age: 8.8 years). In all, the presence of sulfomucins was assessed with Alcian blue-periodic acid-Schiff pH 1.0. Immunoreactivity was graded as absent (0), weak (1 +), moderate (2+), or intense (3+), in accordance with the intensity of the staining, and its distribution as focal or diffuse. CK7 reactivity was 2 + either focal or diffuse in all group A biopsies. The reactivity was more evident in the cells at the neck of the glands, in the areas with more inflammatory infiltrates, decorating long vertical segments of epithelium. In groups B and C, CK7 reactivity was also focal and 1 + at the cells of the necks of the glands. However, group B presented longer vertical segments of positive cells as compared to group C, and shorter than those of group A. The deeper glandular structures were focally 1 + in both groups. CK20 expression was comparable in all three groups, depicting a 2+ diffuse reactivity at the surface epithelium and interposed pits with absence or focal reactivity at the neck and coiled gland areas. Ki-67 immunostaining paralleled that of the CK7. Staining for sulfated mucosubstances was positive in two of five cases of groups A and B, and in none of the cases of group C. We conclude that: (1) the long segments of CK7-positive glandular necks in H. pylori cases most probably indicate intense regenerative activity during active inflammation; (2) eradication of H. pylori does not warrant ad integrum restitution since long segments of Ki-67+, CK7+ cells at the germinative compartment of the glands (as well as cells with sulfomucins) were still recognizable in ex- H. pylori patients; (3) finally, differing from what happens in adults, children somehow manage to maintain fully differentiated CK20+ superficial epithelium while the H. pylori is in action.

Extremely Long Survival under Combined Immunotherapy in a Metastatic Functional Neuroendocrine Neoplasia Patient

2017 ◽  
Vol 106 (4) ◽  
pp. 381-388 ◽  
Author(s):  
Daniel Schmidt ◽  
Bertram Wiedenmann
Keyword(s):  
Advanced Disease ◽  
Residual Disease ◽  
Ki 67 ◽  
Neuroendocrine Neoplasia ◽  
Gastric Type ◽  
Tumor Load ◽  
Stage Ivb ◽  
Multimodality Approach ◽  
Combined Immunotherapy

Treatment and prognosis of neuroendocrine neoplasia depends on tumor size, stage, grade, resectability, and extent of distant metastasis. In most cases a multimodality approach including surgical, locally invasive procedures, peptide-guided radioreceptor therapy (PRRT), and medical therapies represent the mainstay of treatment in advanced disease. In the reported case, a 68-year-old man was diagnosed in 2010 with an initially functional (histamine) neuroendocrine tumor of gastric type III, G2, stage IVB, cT4cN1cM1 (hepatic, peritoneal, nodal, osseous), including a hepatic tumor load of 25%. Intensive multimodality approaches including combined immunotherapy (vaccination and PD-1/CTLA-4 blockade) led to a survival of 8 years until now with a high quality of life and minimal residual disease (only a single, small paragastric recurrence) despite the dedifferentiation of the tumor into a neuroendocrine carcinoma G3 (Ki-67 of 80%) including a nonfunctional stage.

Gastric-Type Adenocarcinoma of the Uterine Cervix

2018 ◽  
Vol 28 (6) ◽  
pp. 1203-1210 ◽  
Author(s):  
Kye Jin Park ◽  
Mi-hyun Kim ◽  
Jeong Kon Kim ◽  
Kyoung-Sik Cho
Keyword(s):  
Uterine Cervix ◽  
Gastric Type

scholarly journals Inovasi Sistem Stack Microbial Fuel Cell menggunakan Substrat Limbah Rumen Sapi serta Implementasinya sebagai Media Pembelajaran

2018 ◽  
Vol 2 (2) ◽  
pp. 188-195
Author(s):  
Diya Novarina ◽  
Eko Swistoro ◽  
M. Lutfi Firdaus ◽  
Rosane Medriati
Keyword(s):  
Fuel Cell ◽  
Electric Power ◽  
Learning Outcomes ◽  
Microbial Fuel Cell ◽  
Mixed Type ◽  
Learning Model ◽  
Anode Surface ◽  
Series Design ◽  
The Difference ◽  
Mixed Types

ABSTRACT[Innovation of microbial fuel cell stack system using cow rumen waste substrate and its implementation as a learning media]. The aims of this study are to: 1) describe the difference of Electric Motion (GGL), 2) describe the difference of electric power per unit area of the anode (Pa) which is produced between the series design MFC type series, parallel, mixed type 1 and mixed type 2, and 3) describe the significant difference of learning outcomes between the PBL learning model using MFC media with PBL learning model by using the electrical kit medium on dynamic electrical concept in SMA Muhammadiyah 4 Bengkulu. The free variable of this research are MFC stack system design (series, parallel, mixed types 1 and 2) and the dependent variable is GGL and electric power per unit of anode surface area. The results are obtained difference GGL and Pa between series design, parallel, mixed types 1 and 2 by comparison are 3: 1: 1,5: 2 whereas Pa ratio is 1: 10: 6: 2 with maximum GGL is obtained in series design of 3,29 V and Pa maximum in parallel circuit 21,76 mW / m2. Implementation of MFC series as a learning media used Problem Based Learning model on physics learning Implementation of MFC circuit with research design using one group pretest-posttest design. The testing of hypothesis with t-test shows tcount 2.739> ttable 2,001 and 95% significant level so it can be concluded there are significant differences in learning outcomes of PBL learning model by using MFC media with PBL learning model by using media Electric Kit on dynamic electrical concept in SMA Muhammadiyah 4 Bengkulu. Keywords: Stack Microbial Fuel Cell (MFC) Sistem; Rumen Cow Liquid Waste; Learning Media.

scholarly journals Evidence for prescribed NK cell Ly49 developmental pathways in mice

2020 ◽  
Author(s):  
Alberto J. Millan ◽  
Bryan A. Hom ◽  
Jeremy B. Libang ◽  
Suzanne Sindi ◽  
Jennifer O. Manilay
Keyword(s):  
Nk Cells ◽  
Nk Cell ◽  
Maturation Stage ◽  
Cell Stage ◽  
Ki 67 ◽  
Mhc I ◽  
Cell Clusters ◽  
Cell Counts

AbstractPrevious studies of NK cell inhibitory Ly49 receptors suggested their expression is stochastic. However, relatively few studies have examined this stochasticity in conjunction with activating Ly49 receptors. We hypothesized that the expression of activating Ly49 receptors is not stochastic and is influenced by inhibitory Ly49 receptors. We analyzed NK cell “clusters” defined by combinatorial expression of activating (Ly49H, Ly49D) and inhibitory (Ly49I, Ly49G2) receptors in C57BL/6 mice. Using the product rule to evaluate the interdependencies of the Ly49 receptors, we found evidence for a tightly regulated expression at the immature NK cell stage, with the highest interdependencies between clusters that express at least one activating receptor. Further analysis demonstrated that certain NK clusters predominated at the immature (CD27+CD11b−), transitional (CD27+CD11b+) and mature (CD27−CD11b−) NK cell stages. Using parallel in vitro culture and in vivo transplantation of sorted NK clusters, we discovered non-random upregulation of Ly49 receptors, suggesting that prescribed pathways of NK cluster differentiation exist. Our data infer that upregulation of Ly49I is an important step in NK cell maturation. Ki-67 expression and cell counts confirmed that immature NK cells proliferate more than mature NK cells. We found that MHC-I is particularly important for regulation of Ly49D and Ly49G2, even though no known MHC-I ligand for these receptors is present in B6 mice. Our data indicate that the regulatory systems controlling the expression of both activating and inhibitory Ly49 receptors are non-stochastic and support the idea that NK cell clusters develop in a non-random process correlated to their maturation stage.

scholarly journals COMPARISON OF IMMUNOHISTOCHEMICAL MARKER WITH SPECIAL STAINS IN THE PROGNOSIS OF THE DIFFERENT GRADES OF ORAL SQUAMOUS CELL CARCINOMA

2019 ◽  
Vol 8 (4) ◽  
Author(s):  
Dr. Parthiban Nallaiyan ◽  
Dr. Bharathi Ramakrishnan ◽  
Dr. Gnanadeepam Santigo ◽  
Dr. Dhanalakshmi Jeyasivanesan
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Periodic Acid ◽  
Collagen Fibre ◽  
Ki 67 ◽  
Periodic Acid Schiff ◽  
Schiff Reagent ◽  
Neutral Mucin

Objectives: The aim of the study is to compare the efficiency of Ki-67 with special stains in the stroma of different grades of Oral Squamous Cell Carcinoma (OSCC) and to evaluate the influence of these changes in predicting the prognosis of these tumors. Materials and Methods: A total of 30 cases of different grades of Oral Squamous Cell Carcinoma and 6 cases of control were sections and stained with Picrosirus red (PR), combined Alcian blue-periodic acid Schiff reagent (AB-PAS) and Immunohistochemical (IHC) marker Ki-67. Results: Collagen fibre nature using PR stain and proliferative activity of malignant epithelial cells using IHC marker Ki-67 was found to be statistically significant. Mucin presence using AB-PAS was statistically insignificant. Conclusion: Prognosis in different grades of oral squamous cell carcinoma can be accessed by change in collagen fibres birefringence, as the tumour progresses there is change from mature to immature collagen. Ki-67 is a good proliferative marker and shows that there is positive correlation with histological grading of oral squamous cell carcinoma. Presence of acidic or neutral mucin in OSCC needed to be further studied. For assessing the prognosis in different grades of OSCC, special stains can also be used. Keywords: Collagen, Mucin, Squamous cell carcinoma, Ki-67.

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