ANALISIS FAKTOR PENGGUNAAN FORMALIN PADA PEDAGANG TAHU DI PASAR TRADISONAL KOTA SERANG

Formalin is known as a disinfectant, germicide and non-food preservative. For this reason, useing formalin in tofu food products is strictly prohibited. The importance of preventing the use of formalin in tofu traders has an impact on human health so it is safe for consumption. The purpose of this study was to determine of formaldehyde used among tofu traders in the traditional market of Serang City. Cross-sectional study with total sampling technique. The research sample was 43 tofu traders and 43 tofu samples were taken. Questionnaire instrument to obtain data on the variables of knowledge, attitudes and supervision variables that had previously been tested for validity (r count > r table) and reliability with Cronbach's alpha (α) value of 0.947 > 0.60. Tofu formalin content examination was carried out by the Regional Health Laboratory (Labkesda) Serang City using the Schiff reagent test. The results showed that there were (67.4%) tofu trader were positive formalin. There is no relationship between attitudes and use of formaldehyde in tofu traders (p=1,000). There is a relationship between knowledge (p=0.016) and supervision (p=0.004) with the use of formalin in tofu traders. Application of regulations, supervision traders and producers to prevent the use of formaldehyde in food.

Death from amniotic fluid embolism in early postpartum period: a case report

This paper presents the case of a 40-year-old woman who died from amniotic fluid embolism on the third day after operative delivery (IV) at 38 weeks of gestation. Histological examination was conducted using staining with hematoxylin and eosin and Schiff reagent. The basis for the postmortem diagnosis was the presence of solid and liquid components of amniotic fluid in the uterine and pulmonary vessels with the absence of morphological substrate of other critical obstetric states.

Detection of Formalin Content And Quality Properties of Snakehead (Channa striata) Dried Salted Fish From The Traditional Markets In Pontianak City In Indonesia

The snakehead fish (Channa striata) dried salted fish is one of the freshwater salted fish favored by local people in Pontianak city, West Kalimantan, Indonesia. The aim of this research was to identify the formalin content and status of the quality properties of the Snakehead dried salted fish in the traditional markets in Pontianak city. Sampling is carried out in five traditional market selected i.e. Mawar, Dahlia, Puring, Kenanga dan Flamboyan. The determination of formalin was carried out by qualitative and quantitative methods using the Schiff reagent. All the samples are appropriate with SNI standard in salt content ranged from 7,08-8,49% and water content ranged from 36,66 – 39,04%. The level of panelists acceptance for appearance score ranged from 6,70–7,06 (whole, clean, slight dullness), the odor score ranged from 6,26 – 6,93 (neutral, slight odor additive - slight natural odor, slight odor additive), and the texture score ranged from 6,48 – 7,36 (solid, some loss of elasticity-very firm, some loss of elasticity). All the samples are contained formalin ranged from 0,64-0,83 ppm. The total plate count (TPC) of the samples was found ranged from 2,1 x 107 - 7,0 x 107 Cfu/g.

The Use of One Normal Hydrochloric Acid as a Background Cleaner for Periodic Acid–Schiff Staining Technique

Objectives Sulfurous rinsing was considered nonessential as contained in the published periodic acid–Schiff technique because it did not clear the background better than tap water washing. The objective of this work is to elucidate the use of 1N HCl as a better background cleaner in the periodic acid–Schiff staining technique. Methods This is an experimental research. Normal samples of antrium, kidney, spleen, muscle, liver, and appendix, as well as histopathologically diagnosed samples of glycogen storage disease of the liver, alpha-1-antitrypsin deficient liver, aspergillosis nasal cavity sample, and glomerulosclerosis samples were obtained from the pathological archive. These samples were stained with a newly purchased commercially prepared Schiff reagent and overstained commercially prepared Schiff’s solution causing nonspecific background staining and laboratory-prepared Schiff reagent. Oxidation was done with 1% periodic acid for 5 minutes, washed for 5 minutes with distilled water, and stained for 10 minutes in Schiff’s reagent, but 20-minute staining was observed for the overstained Schiff reagent, oxidized in running tap water for 15 minutes. It was then rinsed in 1N HCl for 20 seconds, washed in tap water for 2 minutes, and counterstained with hematoxylin for 2 minutes. The same procedure was done for another set of slides as standards without 1N HCl rinsing done. The slides were examined under the microscope and compared for specificity. Results Slides rinsed in 1N HCl showed a clear differentiation of strong, weak, and non-PAS-positive substances. The overall effect of these staining characteristics was more pronounced in the use of laboratory-prepared Schiff. Conclusion The use of 1N HCl as a differentiator made the PAS technique more specific in its staining characteristics, especially with the use of laboratory-prepared Schiff’s reagent, which is commonly used in developing countries owing to a high cost of commercially prepared Schiff’s solution.

COMPARISON OF IMMUNOHISTOCHEMICAL MARKER WITH SPECIAL STAINS IN THE PROGNOSIS OF THE DIFFERENT GRADES OF ORAL SQUAMOUS CELL CARCINOMA

Objectives: The aim of the study is to compare the efficiency of Ki-67 with special stains in the stroma of different grades of Oral Squamous Cell Carcinoma (OSCC) and to evaluate the influence of these changes in predicting the prognosis of these tumors. Materials and Methods: A total of 30 cases of different grades of Oral Squamous Cell Carcinoma and 6 cases of control were sections and stained with Picrosirus red (PR), combined Alcian blue-periodic acid Schiff reagent (AB-PAS) and Immunohistochemical (IHC) marker Ki-67. Results: Collagen fibre nature using PR stain and proliferative activity of malignant epithelial cells using IHC marker Ki-67 was found to be statistically significant. Mucin presence using AB-PAS was statistically insignificant. Conclusion: Prognosis in different grades of oral squamous cell carcinoma can be accessed by change in collagen fibres birefringence, as the tumour progresses there is change from mature to immature collagen. Ki-67 is a good proliferative marker and shows that there is positive correlation with histological grading of oral squamous cell carcinoma. Presence of acidic or neutral mucin in OSCC needed to be further studied. For assessing the prognosis in different grades of OSCC, special stains can also be used. Keywords: Collagen, Mucin, Squamous cell carcinoma, Ki-67.

PERFORMA (PAPER TEST KIT FORMALIN) AS THE ALTERNATIVE SELECTION TO IMPROVE THE QUALITY OF FOOD INGREDIENTS

Research on "PERFORMA" or Paper Test Kits Formalin is as an alternative to improve the quality of food selection. It is aiming to get a paper test kit that can detect formaldehyde contented in foodstuffs practically, fast, easy, and inexpensive. The method used in this study is an experimental method which is part of a quantitative method that aims to manipulate and control variables. The data collection is based on the research and the observation of the color change on the paper test kit is generated from Schiff reagent test, in which the paper test kit will give a purple color response when tested on foodstuffs containing formalin. Based on the analysis of variety of data, it is obtained and expressed as mean ± standard deviation with confidence interval in this study are stated at α 5%. Thus, this research paper expects that formalin test kit can be easily applied and used by the community at large. Keywords: foodstuff, formalin, paper test kits, reagents Schiff