Ex vivo Fusion Confocal Microscopy of Colorectal Polyps: A Fast Turnaround Time of Pathological Diagnosis

Pathobiology ◽  
2021 ◽  
pp. 1-8
Author(s):  
Jose Andres Guerrero ◽  
Javiera Pérez-Anker ◽  
Gloria Fernández-Esparrach ◽  
Ivan Archilla ◽  
Alba Diaz ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Ex Vivo ◽  
Turnaround Time ◽  
Colorectal Polyps ◽  
Diagnostic Efficiency ◽  
Direct Benefit ◽  
Screening Programs ◽  
Hematoxylin And Eosin ◽  
Scanned Images ◽  
Fast Turnaround Time

<b><i>Background:</i></b> Colorectal cancer screening programs have accomplished a mortality reduction from the disease but have created bottlenecks in endoscopy units and pathology departments. We aimed to explore the feasibility of ex vivo fusion confocal microscopy (FuCM) to improve the histopathology diagnostic efficiency and reduce laboratory workload. <b><i>Methods:</i></b> Consecutive fresh polyps removed at colonoscopy were scanned using ex vivo FuCM, then went through histopathologic workout and hematoxylin and eosin (H&amp;E) diagnosis. Two pathologists blinded to H&amp;E diagnosis made a diagnosis based on FuCM scanned images. <b><i>Results:</i></b> Thirty-six fresh polyps from 22 patients were diagnosed with FuCM and H&amp;E. Diagnostic agreement between H&amp;E and FuCM was 97.2% (kappa = 0.96) for pathologist #1 and 91.7% (kappa = 0.87) for pathologist #2. Diagnostic performance concordance between FuCM and H&amp;E to discern adenomatous from nonadenomatous polyps was 100% (kappa = 1) for pathologist #1 and 97.2% (kappa = 0.94) for pathologist #2. Global interobserver agreement was 94.44% (kappa = 0.91) and kappa = 0.94 to distinguish adenomatous from nonadenomatous polyps. <b><i>Conclusions:</i></b> Ex vivo FuCM shows an excellent correlation with standard H&amp;E for the diagnosis of colorectal polyps. The clinical direct benefit for patients, pathologists, and endoscopists allows adapting personalized surveillance protocols after colonoscopy and a workload decrease in pathology departments.

scholarly journals Features of oral squamous cell carcinoma in ex vivo fluorescence confocal microscopy

2020 ◽  
Author(s):  
Veronika Shavlokhova ◽  
Christa Flechtenmacher ◽  
Sameena Sandhu ◽  
Michael Vollmer ◽  
Jürgen Hoffmann ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Confocal Microscopy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ex Vivo ◽  
Fluorescence Confocal Microscopy

scholarly journals In Vivo Confocal Microscopy Evaluation in Patients with Keratoconus

2022 ◽  
Vol 11 (2) ◽  
pp. 393
Author(s):  
Alvin Wei Jun Teo ◽  
Hassan Mansoor ◽  
Nigel Sim ◽  
Molly Tzu-Yu Lin ◽  
Yu-Chi Liu
Keyword(s):  
Confocal Microscopy ◽  
Nerve Regeneration ◽  
Nerve Fibre ◽  
Ex Vivo ◽  
Fibre Length ◽  
In Vivo Confocal Microscopy ◽  
Corneal Sensitivity ◽  
Mechanical Removal ◽  
Cellular Changes

Keratoconus is the most common primary corneal ectasia characterized by progressive focal thinning. Patients experience increased irregular astigmatism, decreased visual acuity and corneal sensitivity. Corneal collagen crosslinking (CXL), a minimally invasive procedure, is effective in halting disease progression. Historically, keratoconus research was confined to ex vivo settings. In vivo confocal microscopy (IVCM) has been used to examine the corneal microstructure clinically. In this review, we discuss keratoconus cellular changes evaluated by IVCM before and after CXL. Cellular changes before CXL include decreased keratocyte and nerve densities, disorganized subbasal nerves with thickening, increased nerve tortuosity and shortened nerve fibre length. Repopulation of keratocytes occurs up to 1 year post procedure. IVCM also correlates corneal nerve status to functional corneal sensitivity. Immediately after CXL, there is reduced nerve density and keratocyte absence due to mechanical removal of the epithelium and CXL effect. Nerve regeneration begins after 1 month, with nerve fibre densities recovering to pre-operative levels between 6 months to 1 year and remains stable up to 5 years. Nerves remain tortuous and nerve densities are reduced. Corneal sensitivity is reduced immediately postoperatively but recovers with nerve regeneration. Our article provides comprehensive review on the use of IVCM imaging in keratoconus patients.

scholarly journals Insertable system for fast turnaround time microwave experiments in a dilution refrigerator

2012 ◽  
Vol 83 (9) ◽  
pp. 093904 ◽  
Author(s):  
Florian R. Ong ◽  
Jean-Luc Orgiazzi ◽  
Arlette de Waard ◽  
Giorgio Frossati ◽  
Adrian Lupascu
Keyword(s):  
Turnaround Time ◽  
Dilution Refrigerator ◽  
Fast Turnaround Time

Evaluation of Morphology and Microcirculation of the Pancreas by ex vivo and in vivo Reflectance Confocal Microscopy

Pancreatology ◽  
2001 ◽  
Vol 1 (1) ◽  
pp. 48-57 ◽  
Author(s):  
Tobias Keck ◽  
Vanessa Campo-Ruiz ◽  
Andrew L. Warshaw ◽  
R. Rox Anderson ◽  
Carlos Fernández-del Castillo ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Ex Vivo ◽  
Reflectance Confocal Microscopy

In Vivo and Ex Vivo Laser Confocal Microscopy Findings in Patients With Early-stage Acanthamoeba Keratitis

Cornea ◽  
2008 ◽  
Vol 27 (4) ◽  
pp. 439-445 ◽  
Author(s):  
Akira Kobayashi ◽  
Yasuhisa Ishibashi ◽  
Yosaburo Oikawa ◽  
Hideaki Yokogawa ◽  
Kazuhisa Sugiyama
Keyword(s):  
Confocal Microscopy ◽  
Ex Vivo ◽  
Early Stage ◽  
Acanthamoeba Keratitis ◽  
Laser Confocal Microscopy

In vivo and ex vivo confocal microscopy of eyelid melanoma: case report

2014 ◽  
Vol 92 ◽  
pp. 0-0
Author(s):  
M HAOUAS ◽  
C GUILLEMOT ◽  
D GRIVET ◽  
E CINOTTI ◽  
JL PERROT ◽  
...  
Keyword(s):  
Case Report ◽  
Confocal Microscopy ◽  
Ex Vivo ◽  
Melanoma Case

scholarly journals High-volume workflow and performance comparisons for Chlamydia trachomatis and Neisseria gonorrhoeae testing using automated molecular platforms

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
André Frontzek ◽  
Gudrun Aretzweiler ◽  
Daniela Winkens ◽  
Dana Duncan ◽  
Elizabeth M. Marlowe
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
High Volume ◽  
Turnaround Time ◽  
Screening Programs ◽  
Percent Agreement ◽  
Significant Difference ◽  
Mcnemar’S Test ◽  
And Performance ◽  
Mcnemar's Test

Abstract Background The global burden of sexually transmitted infections (STIs) is high and there have been reports of increasing chlamydial and gonorrheal infections. High-volume screening programs for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are an important component of STI control. This study evaluated the high-volume workflow and performance of the cobas® CT/NG assay for use on the automated Roche cobas® 6800 system, with the cobas p 480 instrument for pre-analytics, compared with the Aptima Combo 2 assay on the Hologic Panther system. Methods High-volume workflow and performance were evaluated using paired female urine specimens. Workflow analysis (n = 376) included hands-on time (HoT), number of manual interventions, and time to first and last results. For performance assessment, paired results from the cobas CT/NG and Aptima Combo 2 assays, for both CT and NG, were compared and two-sided 95% confidence intervals calculated to provide estimates of positive percent agreement (PPA), negative percent agreement (NPA), and overall percent agreement (OPA) between the tests. McNemar’s test was used for significance testing. Results Pre-analytical preparations and system start-up on the cobas 6800 system required 00:27:38 (hr:min:sec) HoT whilst the Panther system required 00:30:43. The cobas 6800 system required eight interactions and 00:43:59 HoT to process 376 samples. The Panther system required six interactions and 00:39:10 HoT. Time to first results was 02:53:00 on the cobas c6800 system for 96 samples and 03:28:29 on the Panther system for five samples. The cobas 6800 system delivered all 376 results 3 h faster than the Panther system (07:45:26 and 10:47:30, respectively). The performance correlation between both assays was high (PPA, NPA and OPA > 99% for both CT and NG). McNemar’s test revealed no statistically significant difference between the assays. Conclusion For high-volume automated CT/NG testing, both the cobas 6800 system and Panther system provided accurate results. Although less manual intervention steps were needed for the Panther system, improved turnaround time was obtained with the cobas 6800 system with less risk for contamination. The additional testing capacity on the cobas 6800 system would allow a growing service to deliver more results in a single shift.

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