Targeted mass spectrometry analysis of neutrophil-derived proteins released during sepsis progression

2014 ◽  
Vol 112 (12) ◽  
pp. 1230-1243 ◽  
Author(s):  
Alzbeta Davidova ◽  
Matthias Mörgelin ◽  
Adam Linder ◽  
Michael Larsen ◽  
Klaus Qvortrup ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Infectious Diseases ◽  
Polymorphonuclear Neutrophils ◽  
Mass Spectrometry Analysis ◽  
Selected Reaction Monitoring ◽  
Abundance Pattern ◽  
Spectrometry Analysis ◽  
Sepsis Diagnosis ◽  
Highly Sensitive ◽  
Novel Biomarkers

SummaryEarly diagnosis of severe infectious diseases is essential for timely implementation of lifesaving therapies. In a search for novel biomarkers in sepsis diagnosis we focused on polymorphonuclear neutrophils (PMNs). Notably, PMNs have their protein cargo readily stored in granules and following systemic stimulation, an immediate increase of neutrophil-borne proteins can be observed into the circulation of sepsis patients. We applied a combination of mass spectrometry (MS) based approaches, LC-MS/MS and selected reaction monitoring (SRM), to characterise and quantify the neutrophil proteome in healthy or disease conditions. With this approach we identified a neutrophil- derived protein abundance pattern in blood plasma consisting of 20 proteins that can be used as a protein signature for severe infectious diseases. Our results also show that SRM is highly sensitive, specific, and reproducible and, thus, a promising technology to study a complex, dynamic and multifactorial disease such as sepsis.

Diamond nanowires for highly sensitive matrix-free mass spectrometry analysis of small molecules

Nanoscale ◽  
2012 ◽  
Vol 4 (1) ◽  
pp. 231-238 ◽  
Author(s):  
Yannick Coffinier ◽  
Sabine Szunerits ◽  
Hervé Drobecq ◽  
Oleg Melnyk ◽  
Rabah Boukherroub
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Highly Sensitive ◽  
Matrix Free

scholarly journals The Cardiac Lipidome in Models of Cardiovascular Disease

Metabolites ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 254 ◽  
Author(s):  
Mateusz M. Tomczyk ◽  
Vernon W. Dolinsky
Keyword(s):  
Mass Spectrometry ◽  
Cardiovascular Disease ◽  
Molecular Mechanisms ◽  
Cell Structure ◽  
Myocardial Cell ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Cardiac Lipid ◽  
Lipid Species ◽  
Novel Biomarkers

Cardiovascular disease (CVD) is the leading cause of death worldwide. There are numerous factors involved in the development of CVD. Among these, lipids have an important role in maintaining the myocardial cell structure as well as cardiac function. Fatty acids (FA) are utilized for energy, but also contribute to the pathogenesis of CVD and heart failure. Advances in mass spectrometry methods have enabled the comprehensive analysis of a plethora of lipid species from a single sample comprised of a heterogeneous population of lipid molecules. Determining cardiac lipid alterations in different models of CVD identifies novel biomarkers as well as reveals molecular mechanisms that underlie disease development and progression. This information could inform the development of novel therapeutics in the treatment of CVD. Herein, we provide a review of recent studies of cardiac lipid profiles in myocardial infarction, obesity, and diabetic and dilated cardiomyopathy models of CVD by methods of mass spectrometry analysis.

Highly sensitive protein detection by combination of atomic force microscopy fishing with charge generation and mass spectrometry analysis

FEBS Journal ◽  
2014 ◽  
Vol 281 (20) ◽  
pp. 4705-4717 ◽  
Author(s):  
Yuri D. Ivanov ◽  
Tatyana Pleshakova ◽  
Krystina Malsagova ◽  
Andrey Kozlov ◽  
Anna Kaysheva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Protein Detection ◽  
Mass Spectrometry Analysis ◽  
Charge Generation ◽  
Spectrometry Analysis ◽  
Force Microscopy ◽  
Atomic Force ◽  
Highly Sensitive

scholarly journals Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Takashi Kanamoto ◽  
Takashi Tachibana ◽  
Yasushi Kitaoka ◽  
Toshio Hisatomi ◽  
Yasuhiro Ikeda ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ocular Hypertension ◽  
Aspartic Acid ◽  
Atp Synthase ◽  
Wistar Rats ◽  
Protein Band ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Sds Page

Purpose. To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins. Methods. Adult Wistar rats with ocular hypertension were used as an experimental model. D-β-aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D-β-aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis. The concentration of ATP was measured by ELISA. Results. D-β-aspartic acid was expressed in a protein band at around 44.5 kDa at much higher quantities in the retinas of rats with ocular hypertension than in those of normotensive rats. The 44.5 kDa protein band was mainly composed of α-enolase, S-arrestin, and ATP synthase subunits α and β, in both the ocular hypertensive and normotensive retinas. Moreover, increasing intraocular pressure was correlated with increasing ATP concentrations in the retinas of rats. Conclusion. Ocular hypertension affected the expression of proteins containing D-β-aspartic acid, including ATP synthase subunits, and up-regulation of ATP in the retinas of rats.

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