Abstract 13039: Loss of Visceral Adipose Tissue Macrophage Subsets Induces Myocardial Infarction Induced Insulin Resistance: Role of Adiponectin

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Sathish Babu B Vasamsetti ◽  
xinyi zhang ◽  
Emillie M Coppin ◽  
Jonathan Florentin ◽  
Sasha Koul ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Insulin Resistance ◽  
Adipose Tissue ◽  
Blood Glucose ◽  
Visceral Adipose Tissue ◽  
Fasting Blood Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Systemic Insulin Resistance ◽  
Visceral Adipose

Introduction: Myocardial infarction (MI) is the major cause of morbidity and mortality in the western world. Insulin resistance is a major complication in patients with MI. Hypothesis: Loss of visceral adipose tissue (VAT) resident macrophages in MI results in diminished adiponectin production causing systemic insulin resistance. Methods: To understand if MI results in insulin resistance, we analyzed UPMC patient records and identified patients who had normal fasting blood glucose levels on average 15 days before ST elevation myocardial infarction (STEMI) and checked their fasting blood glucose levels 30 days after STEMI. To understand the mechanisms of MI-induced insulin resistance, we used a mouse model of coronary ligation in C57BL/6 mice and analyzed the features of insulin resistance by measuring serum insulin, serum adiponectin, AKT activation status in the liver and muscle. Results: We found that 50% of non-diabetic patients (fasting blood glucose levels 99±2.5 mg/ dl) developed hyperglycemia (141±13 mg/dl) after MI, suggesting that MI causes insulin resistance. Consistently, mice with MI had higher fasting blood insulin, and reduced p-Akt levels in the liver and skeletal muscles confirming insulin resistance. Concomitantly, mice and patients with MI had reduced number of visceral adipose tissue (VAT) resident macrophages. In line with this, MI resulted in marked reduction in the level of macrophage colony stimulating factor (M-CSF), a cytokine required for tissue resident macrophage survival. M-CSF supplementation in mice with MI improved insulin sensitivity and decreased inflammatory phenotype of VAT macrophages. Furthermore, the systemic level of adiponectin, which is reported to augment insulin sensitivity, was profoundly reduced in mice after MI. Specific depletion of VAT resident macrophages resulted in lower levels of adiponectin in the serum, indicating that this macrophage subset is necessary for adiponectin production by adipocytes. Conclusions: Our data demonstrate that diminished M-CSF levels after MI triggers apoptosis of VAT resident macrophages, resulting in reduced adiponectin secretion and systemic insulin resistance.

scholarly journals Effect of Insulin Resistance on Lipolytic activity of Adipose Tissue in Male Rats

2012 ◽  
Vol 1 (2) ◽  
pp. 68-73
Author(s):  
R Eldeeb ◽  
MH Gamal-Eldin ◽  
EA Khowailed ◽  
MM Fathy ◽  
N Shantakumari ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Blood Pressure ◽  
Body Weight ◽  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
Lipolytic Activity ◽  
Fasting Blood Glucose ◽  
Significant Rise ◽  
Male Rats ◽  
Visceral Adipose

Background: The excess usage of fructose as a sweetener has raised the incidence of insulin resistance among the population which is associated with dyslipedemia, hypertension and obesity. This work studied the effect of induced insulin resistance on body weight, blood pressure, lipid profile, glycemic state and lipolytic activity of adipose tissue in male rats. Methods: Twenty male rats of 129.4 g average body weight (BW) were divided equally into two groups. Both had free access to water. The control group had pure water; the experimental group had water mixed with 25% of fructose to induce insulin resistance. After 3 months body weight, blood pressure, fasting blood glucose, insulin levels, lipid profile of both groups were measured and lipolytic activity of adipose tissue was assessed. Results: Rats given fructose for 3 months showed significant increase in BW, systolic blood pressure, triglyceride, Cholesterol, low density lipoprotein, fasting blood glucose and insulin levels with a significant decline in highdensity lipoprotein. Lipolytic activity of subcutaneous (SC) and visceral adipose tissue in presence of adrenaline increased significantly which runs in parallel with the results obtained in presence of insulin as it showed a significant rise in both SC and visceral adipose tissue. Data were considered statistically significant at alpha level of 5%. Conclusion: Insulin resistance induced in male rat by high fructose consumption showed a significant rise in BW and is associated with hypertension and dyslipidemia with significant rise in lipolytic activity of both SC and visceral adipose tissue. DOI: http://dx.doi.org/10.3126/njms.v1i2.6602 Nepal Journal of Medical Sciences. 2012;1(2): 68-73

scholarly journals Effect of Celery Extract on Fructose Induced Insulin Resistance Rattus norvegicus

2020 ◽  
Vol 56 (4) ◽  
pp. 269
Author(s):  
Devitya Angielevi Sukarno ◽  
Arifa Mustika ◽  
Purwo Sri Rejeki
Keyword(s):  
Insulin Resistance ◽  
Blood Glucose ◽  
Treatment Group ◽  
Rattus Norvegicus ◽  
Therapy Group ◽  
Fasting Blood Glucose ◽  
Apium Graveolens ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus (DM) is one of the global health problems and in the top 4th ranks as the main cause of death in developing countries (IDF, 2015). The pathogenesis of type II DM involves abnormalities in insulin secretion and activity that leads to insulin resistance. This research aims to study the efficacy of celery (Apium graveolens) as a prevention of insulin resistance. In this study, the samples were 45 Wistar rats (Rattus norvegicus), male sex, aged 4-6 weeks, weight 150-175 grams, and had normal fasting blood glucose levels by tested before treatment. The experimental animals were divided into 5 groups, K1 was negative control group (insulin resistance by given 20% fructose 1,86 g/kg BW PO qDay); K2 was positive control group (those given 20% fructose 1,86 g/kg BW PO qDay and standard insulin resistance therapy metformin 500 mg/kg BW PO qDay); the K3 treatment group was given 20% fructose 1,86 g/kg BW PO qDay and celery extract 200 mg/kg BW PO qDay; the K4 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 400 mg/kg BW PO qDay; and the K5 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 600 mg/kg BW PO qDay. The treatment had been given every day for 60 days. Fasting blood glucose levels were measured using a Glucometer. Fasting blood insulin levels were measured using ELISA, HOMA-IR was calculated using a standardized formula, and GLUT4 protein expression was measured using immunohistochemistry. It the end of the intervention, there was a significant decreased in fasting blood glucose (FBG) in K4 group compared with K1 (p <0.05), insulin resistance in K1 was characterized by a higher HOMA-IR value compared to the therapy group, especially K4 and K5 (p <0.05). There was an increase in GLUT-4 expression on K4 and K5 compared with K1 (p<0.05). It can be concluded that celery extract has antihyperglycemia effect and furthermore it can prevent insulin resistance condition.

scholarly journals Effect of Celery Extract on Fructose Induced Insulin Resistance Rattus norvegicus

2021 ◽  
Vol 56 (4) ◽  
pp. 269
Author(s):  
Devitya Angielevi Sukarno ◽  
Arifa Mustika ◽  
Purwo Sri Rejeki
Keyword(s):  
Insulin Resistance ◽  
Blood Glucose ◽  
Treatment Group ◽  
Rattus Norvegicus ◽  
Therapy Group ◽  
Fasting Blood Glucose ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus (DM) is one of the global health problems and in the top 4th ranks as the main cause of death in developing countries (IDF, 2015). The pathogenesis of type II DM involves abnormalities in insulin secretion and activity that leads to insulin resistance. This research aims to study the efficacy of celery (Apiumgraveolens) as a prevention of insulin resistance. In this study, the samples were 45 Wistar rats (Rattus norvegicus), male sex, aged 4-6 weeks, weight 150-175 grams,and had normal fasting blood glucose levels by tested before treatment. The experimental animals were divided into 5 groups, K1 was negative control group (insulin resistance by given 20% fructose 1,86 g/kg BW PO qDay);K2 was positive control group (those given 20% fructose 1,86 g/kg BW PO qDayand standard insulin resistance therapy metformin 500 mg/kg BW PO qDay); the K3 treatment group was given 20% fructose 1,86 g/kg BW PO qDay and celery extract 200 mg/kg BW PO qDay; the K4 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 400 mg/kg BW PO qDay; and the K5 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 600 mg/kg BW PO qDay. The treatment had been given every day for 60 days.Fasting blood glucose levels were measured using a Glucometer. Fasting blood insulin levels were measured using ELISA, HOMA-IR was calculated using a standardized formula, and GLUT4 protein expression was measured using immunohistochemistry. It the end of the intervention, there was a significant decreased in fasting blood glucose (FBG) in K4 group compared with K1 (p <0.05), insulin resistance in K1 was characterized by a higher HOMA-IR value compared to the therapy group, especially K4 and K5 (p <0.05). There was an increase in GLUT-4 expression on K4 and K5 compared with K1 (p <0.05). It can be concluded that celery extract has antihyperglycemia effect and furthermore it can prevent insulin resistance condition.

scholarly journals CORRELATION BETWEEN VISCERAL ADIPOSE TISSUE-DERIVED SERPIN WITH FASTING BLOOD GLUCOSE LEVEL IN OBESITY

2018 ◽  
Vol 24 (1) ◽  
pp. 19
Author(s):  
Novi Khila Firani ◽  
Agustin Iskandar ◽  
Anik Widijanti ◽  
Nonong Eriani
Keyword(s):  
Adipose Tissue ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Visceral Adipose Tissue ◽  
Fasting Blood Glucose ◽  
Sandwich Elisa ◽  
Fasting Blood Glucose Level ◽  
Visceral Adipose

Abnormalitas jaringan lemak pada kegemukan berhubungan dengan timbulnya berbagai masalah kesehatan, antara lain terjadinyaresistensi insulin. Adipositokin merupakan protein yang dihasilkan jaringan lemak, salah satunya adalah Visceral Adipose Tissue-DerivedSerpin (Vaspin). Beberapa penelitian menunjukkan vaspin berhubungan dengan kepekaan insulin. Belum diketahui apakah dalam setiappeningkatan derajat kegemukan terdapat perubahan hasilan vaspin, yang berhubungan dengan perubahan kadar glukosa darah, sebagaimanifestasi gangguan kepekaan insulin. Rancangan penelitian adalah potong silang, dengan jumlah sampel 60 orang usia dewasa,yang terbagi berdasarkan patokan WPRO (2000), yaitu 10 orang non-kegemukan, 10 orang kegemukan I dan 40 orang tergolongkegemukan II. Pemeriksaan kadar glukosa darah puasa menggunakan metode heksokinase. Pemeriksaan kadar vaspin menggunakanmetode sandwich ELISA. Telitian menunjukkan kadar vaspin di kelompok kegemukan II dan I lebih tinggi dibandingkan kadar vaspinpada non-kegemukan (p=0,00). Kadar vaspin di kegemukan II dan I tidak berbeda bermakna. Kadar glukosa darah puasa di kelompokkegemukan II dan I lebih tinggi dibandingkan kadar glukosa darah puasa di non-kegemukan (p=0,017), namun kadar glukosa darahsebagian besar subjek penelitian masih dalam taraf normal. Hasil uji kenasaban Spearman menunjukkan ada kenasaban positif yangbermakna kadar vaspin terhadap kadar glukosa darah, namun kekuatannya rendah (r=0,384, p=0,001). Terdapat hubungan yangbermakna kadar vaspin dengan kadar glukosa darah puasa di kegemukan. Perlu dilakukan kajian lebih lanjut menggunakan subjekpenelitian kegemukan dengan mengukur indeks kepekaan insulin, untuk memperjelas hubungan antara vaspin, sebagai adipositokinyang berperan dalam kepekaan insulin, terhadap kadar glukosa darah.

Sign in / Sign up

Export Citation Format

Share Document