Morphological and molecular characterisation of Bursaphelenchus andrassyi sp. n. (Nematoda: Aphelenchoididae) from Romania and Turkey

Bursaphelenchus andrassyi sp. n., found in conifer wood samples from Romania and Turkey, is characterised morphologically and genetically. Bursaphelenchus andrassyi sp. n. clearly belongs to the sexdentati group, having a terminal bursa, four lateral lines, a very small female ‘vulval flap’, strongly arcuate spicules and the typical position of caudal papillae of males. It is morphologically most similar to B. vallesianus and B. sexdentati. It can be differentiated from B. vallesianus by the usually subcylindrical female tail with rounded or wedge-shaped terminus vs conical female tail with a more or less rounded terminus, slightly different shape of spicules (low square condylus, lacking a distinct cucullus, pointed rostrum) and from B. sexdentati by lacking a distinct post-vulval constriction, shorter stylet and shorter spicules. The species status is supported by ITS-RFLP patterns and sequencing results of both partial 18S and 28S rDNA regions.

Download Full-text

Radopholoides japonicus n. sp. (Nematoda: Pratylenchidae) found in rhizosphere soil associated with Podocarpus macrophyllus from Japan

Nematology ◽

10.1163/15685411-00003109 ◽

2017 ◽

Vol 19 (9) ◽

pp. 1095-1105

Author(s):

Munawar Maria ◽

Jianfeng Gu ◽

Yiwu Fang ◽

Jie He ◽

Pablo Castillo ◽

...

Keyword(s):

Rhizosphere Soil ◽

Soil Samples ◽

Molecular Characterisation ◽

Maximum Likelihood Analysis ◽

Rhizospheric Soil ◽

Long Tail ◽

Likelihood Analysis ◽

Lateral Lines ◽

Podocarpus Macrophyllus ◽

Partial 18S

Radopholoides japonicus n. sp. isolated from rhizospheric soil samples associated with Podocarpus macrophyllus imported from Japan into Ningbo Port of China is described and illustrated. It is characterised by four lateral lines, lip region low, convex with three annuli. Stylet 16.5-18.5 μm long with anteriorly sloping rounded knobs. Excretory pore at the level of pharyngo-intestinal junction. Vulva posteriorly located, vulval lips not protruding, spermatheca squarish. Post-vulval uterine sac 35-44 μm long. Tail conoid with broadly rounded terminus, phasmid 6-8 annuli posterior to anus. Among five species of genus Radopholoides, the new species is close to R. antoni but can be differentiated from it by lip and tail morphology. This is the first Radopholoides species that provided detailed morphological and molecular characterisation. Maximum Likelihood analysis using the Shimodaira-Hasegawa test for the validity of Radopholoides was performed and showed the validity of the genus using the partial 18S and D2-D3 expansion segments of 28S.

Download Full-text

Molecular characterisation of two known species of Paratylenchus Micoletzky, 1922 from Iran with notes on the validity of Paratylenchus audriellus Brown, 1959

Nematology ◽

10.1163/15685411-00002979 ◽

2016 ◽

Vol 18 (5) ◽

pp. 591-604 ◽

Cited By ~ 4

Author(s):

Mehrab Esmaeili ◽

Ramin Heydari ◽

Pablo Castillo ◽

Mozhgan Ziaie Bidhendi ◽

Juan E. Palomares-Rius

Keyword(s):

18S Rrna ◽

First Record ◽

18S Rrna Gene ◽

Rrna Gene ◽

28S Rrna ◽

Molecular Characterisation ◽

Female Tail ◽

Valid Taxon ◽

Two Populations ◽

Partial 18S

During a survey on pin nematodes in western Iran, two populations of Paratylenchus audriellus and Paratylenchus tenuicaudatus were collected and subsequently analysed morphologically and molecularly. Paratylenchus audriellus is characterised by the long stylet (48-61 μm) and the typical female tail with a characteristic claw-like process with sharply pointed terminus. To our knowledge, the Iranian population of P. tenuicaudatus is the first record from Iran. The molecular characterisation of P. audriellus nematodes using the D2-D3 of 28S rRNA and the partial 18S rRNA gene sequences revealed that this species is clearly separated from P. straeleni and should be considered as a valid taxon.

Download Full-text

Redescription of Bursaphelenchus eucarpus Rühm, 1956 (Nematoda: Aphelenchoididae) associated with the apple bark beetle, Scolytus mali Bechstein, and the shothole borer, S. rugulosus Müller

Nematology ◽

10.1163/15685411-00003183 ◽

2018 ◽

Vol 20 (9) ◽

pp. 889-903 ◽

Cited By ~ 2

Author(s):

Jianfeng Gu ◽

Marek Tomalak ◽

Helen Braasch ◽

Yiwu Fang

Keyword(s):

Bark Beetle ◽

Related Species ◽

Original Description ◽

Morphological Features ◽

Prunus Padus ◽

Female Tail ◽

Lateral Lines ◽

The Common ◽

Tail Tip ◽

Partial 18S

Summary New morphological, molecular and bionomic data are added to update the description of Bursaphelenchus eucarpus. The nematode was isolated from larval galleries and adults of the apple bark beetle, Scolytus mali, and the shothole borer, S. rugulosus, present under the bark of the bird cherry, Prunus padus, and the common European plum, P. domestica, in Poland. Detailed male, female and dispersal juvenile morphology and morphometrics are documented and compared with the original description. From partial 18S, ITS1/2 and partial 28S D2-D3 sequences and morphological features (three lateral lines, position of caudal papillae, shape of the delicate spicules with dorsally bent condylus, lack of vulval flap, a long post-uterine branch and a hyaline region at the female tail tip) it can be concluded that B. eucarpus belongs to the eremus group of Bursaphelenchus. The differentiation from related species of the eremus group is discussed.

Download Full-text

Bursaphelenchus saudi n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from Saudi Arabia

Nematology ◽

10.1163/15685411-00002971 ◽

2016 ◽

Vol 18 (4) ◽

pp. 475-488 ◽

Cited By ~ 2

Author(s):

Jianfeng Gu ◽

Munawar Maria ◽

Yiwu Fang ◽

Jie He ◽

Helen Braasch ◽

...

Keyword(s):

Saudi Arabia ◽

Excretory Pore ◽

Gene Sequencing ◽

Nerve Ring ◽

Precloacal Papilla ◽

Rdna Gene ◽

Female Tail ◽

Lateral Lines ◽

Partial 18S

Bursaphelenchus saudi n. sp., isolated in Ningbo, P.R. China, from Pinus packaging wood imported from Saudi Arabia, is described. It is characterised by four lateral lines, excretory pore located between metacorpus and nerve ring (anterior to median bulb before cultured), presence of a thick vulval flap, post-uterine branch ca two-thirds of the vulva-anus distance long, female tail hook-like (slightly ventrally bent before cultured), long and conical, tapering to a bluntly rounded terminus, spicules transversely striated with weakly developed rostrum and condylus, a single precloacal papilla and three pairs of ventro-sublateral papillae (one pair precloacal, two pairs postcloacal just anterior to bursal flap and adjacent to each other). It belongs to the africanus-group of the genus Bursaphelenchus and is most close to B. paraburgeri, B. burgermeisteri and B. obeche. It is distinguished from other Bursaphelenchus species by morphology, ITS-RFLP patterns and partial 18S, ITS and 28S D2-D3 rDNA gene sequencing results.

Download Full-text

Redescription of Bursaphelenchus parapinasteri (Tylenchina: Aphelenchoididae) isolated from Pinus thunbergii in China with a key to the hofmanni-group

Nematology ◽

10.1163/15685411-00003006 ◽

2016 ◽

Vol 18 (8) ◽

pp. 933-947 ◽

Cited By ~ 4

Author(s):

Munawar Maria ◽

Yiwu Fang ◽

Jianfeng Gu ◽

Hongmei Li

Keyword(s):

Phylogenetic Analyses ◽

Pinus Thunbergii ◽

Zhejiang Province ◽

Female Gonad ◽

Molecular Characterisation ◽

Gene Sequences ◽

Key To Species ◽

Female Tail ◽

Diagnostic Key ◽

Partial 18S

Bursaphelenchus parapinasteriis redescribed morphologically and with new molecular characterisation. It was isolated from a deadPinus thunbergiion Changgang Mountain, Zhoushan Islands, Zhejiang Province, China. Detailed morphology of the spicule, female gonad, hemizonid position, arrangement of male caudal papillae, and female tail terminus shape are documented. The ITS-RFLP patterns and the ITS1/2, partial 28S D2-D3 and partial 18S gene sequences were characterised. The phylogenetic analyses revealed thatB. parapinasteribelongs to thehofmanni-groupsensuBraasch and is close toB. anamurius,B. hofmanni,B. mazandaranense,B. paracorneolus,B. pinasteriandB. ulmophilus. A diagnostic key to species of thehofmanni-group is presented.

Download Full-text

Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P.convallariae and P.fallax (Nematoda: Pratylenchidae)

Nematology ◽

10.1163/156854101753389220 ◽

2001 ◽

Vol 3 (6) ◽

pp. 607-618 ◽

Cited By ~ 16

Author(s):

Lynn Carta ◽

Zafar Handoo ◽

Andrea Skantar

Keyword(s):

New Species ◽

Festuca Arundinacea ◽

Poa Pratensis ◽

28S Rdna ◽

Molecular Characterisation ◽

Lateral Field ◽

Molecular Sequences ◽

Lesion Nematodes ◽

Lateral Lines

AbstractPratylenchus arlingtoni n. sp. from the rhizosphere of grasses Poa pratensis and Festuca arundinacea at Arlington National Cemetery, VA, USA is characterised by six to eight lines in the lateral field, and pyriform to slightly overlapping pharyngeal glands. Morphological comparisons are made with lesion nematodes having similar morphometrics, six lateral lines, or crenate tail tips. Molecular sequences of the LS 28S rDNA were generated for the new species as well as P.fallax and P.convallariae. The new species differs by only 1% from identical sequences found in P.fallax and P.convallariae.

Download Full-text

Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan

Nematology ◽

10.1163/156854108783900294 ◽

2008 ◽

Vol 10 (3) ◽

pp. 335-346 ◽

Cited By ~ 30

Author(s):

Hongmei Li ◽

Phap Quang Trinh ◽

Lieven Waeyenberge ◽

Maurice Moens

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Body Size ◽

Posterior Margin ◽

Petri Dish ◽

Packaging Materials ◽

Lateral Field ◽

Female Tail ◽

Domain Sequence ◽

Lateral Lines

Abstract Bursaphelenchus chengi sp. n. is described and illustrated. Dauer juveniles were isolated from imported wood packaging materials from Taiwan to Nanjing Port, China. Bursaphelenchus chengi sp. n. was reared and maintained on Petri dish cultures of the fungus Botrytis cinerea. The new species is characterised by the medium body size in both sexes, the presence of only two incisures in the lateral field and the robust and strongly curved spicules. The spicule lamina is angular distally, the rostrum digitate and the condylus rounded. The tail is arcuate with a pointed terminus. The bursa is usually truncate with the posterior margin indented in some specimens or rounded with a fine axial point. Females have a small vulval flap formed by a short extension of the cuticle of the anterior lip, and a conical tail that gradually tapers to an almost straight or slightly recurved, pointed or rounded terminus. Because of the presence of two lateral lines, similar spicule shape, tapering female tail and the presence of a small vulval flap, B. chengi sp. n. should be grouped in the abietinus-group sensu Braasch. together with B. abietinus, B. antoniae, B. hellenicus, B. hylobianum and B. rainulfi. ITS-RFLP profiles support the proposal of the new species, and phylogenetic analysis of the 28S rDNA D2/D3 domain sequence places it close to B. antoniae and other species of the abietinus-group.

Download Full-text

Morphological and molecular characterisation of Helicotylenchus ciceri n. sp. and H. scoticus Boag & Jairajpuri, 1985 (Nematoda: Hoplolaimidae) from Iran

Nematology ◽

10.1163/15685411-00003326 ◽

2020 ◽

Vol 22 (6) ◽

pp. 611-626

Author(s):

Fariba Mohammadi Zameleh ◽

Akbar Karegar ◽

Reza Ghaderi ◽

Abbas Mokaram Hesar

Keyword(s):

New Species ◽

Excretory Pore ◽

18S Rrna ◽

Phylogenetic Analyses ◽

Rrna Genes ◽

28S Rrna ◽

Molecular Characterisation ◽

Closely Related Species ◽

Partial 18S ◽

Molecular Characters

Summary Helicotylenchus ciceri n. sp. and H. scoticus are described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by a conical and truncated lip region with five or six distinct annuli, stylet 32-37 μm long with anteriorly concave knobs, secretory-excretory pore posterior to the pharyngo-intestinal valve, dorsally convex-conoid tail with a terminal projection, phasmids 14 (7-20) annuli anterior to the level of anus, empty spermatheca and absence of males. Intraspecific variation of 16 populations of H. scoticus, collected from chickpea and lentil fields in Kermanshah province, western Iran, is discussed. The results of the phylogenetic analyses based on the sequences of the partial 18S rRNA, D2-D3 expansion segments of 28S rRNA and ITS rRNA genes are provided for the studied species, confirming their differences from each other and determining the position of them and their relationships with closely related species.

Download Full-text

Recharacterisation and photo-documentation of five North American Bursaphelenchus species from C.L. Massey’s type material

Nematology ◽

10.1163/15685411-00002961 ◽

2016 ◽

Vol 18 (3) ◽

pp. 311-336 ◽

Cited By ~ 2

Author(s):

Natsumi Kanzaki ◽

Robin M. Giblin-Davis ◽

Barbara J. Center

Keyword(s):

Species Complex ◽

Morphological Traits ◽

Morphological Characters ◽

Female Characters ◽

Species Status ◽

Type Specimens ◽

Lateral Field ◽

Female Tail ◽

Photo Documentation ◽

The Status

The type specimens of fiveBursaphelenchusspecies described by Massey,i.e.,B. bestiolus,B. corneolus,B. elytrus,B. newmexicanusandB. wilfordi, were re-examined and photo-documented to update their descriptions in light of a contemporary understanding of the genus. The presence of a lateral field, basal swelling of stylet, P1 ventral single papilla of males and a pair of three-celled structures in females, which were not described or mentioned in the original descriptions, were observed, although the number of lateral incisures were not clarified because of sample condition. Within these five species, the intrageneric groupings ofB. bestiolusandB. wilfordihad been unclear. In the present study, because of newly observed morphological characters,B. bestiolusis considered to belong to thehofmanni-group, and is close toB. talonusandB. rufipennis. These three species form a species complex and are distinguished from each other only by female tail morphology. The species status ofB. wilfordiis still unclear, although, based on spicule morphology and structure and arrangement of genital papillae, we consider it to belong to thehofmanni-group, close toB. parvispicularis,B. paraparvispicularisandB. osumiana. However, female characters ofB. wilfordiare also similar to members of thecocophilus-group andParasitaphelenchus. Re-isolation and molecular identification are necessary to confirm the status of these species. By contrast, the affiliations ofB. corneoluswith thehofmanni-group andB. elytrusandB. newmexicanuswith theeggersi-group were confirmed. Based upon its morphological traits,B. corneolusis close toB. gerberaeandB. paracorneolus, and is distinguished from these species by its spicule morphology, female tail morphology and female post-uterine sac length. However, male specimens ofB. corneoluswere not available for the present study and confirmation is necessary.Bursaphelenchus elytrusis similar toB. tusciaeand is distinguished from it by its spicule (condylus) morphology and excretory pore position.Bursaphelenchus newmexicanusis close toB. glochisand is distinguished from it mostly by morphometrics and spicule (condylus) morphology. However, morphometrics usually vary among individuals and populations of a species, necessitating morphometric and molecular comparisons of more isolates (strains) in order to clarify specific status.

Download Full-text

Myxidium shedkoae Sokolov, 2013 (Myxozoa: Myxidiidae), a parasite of the gallbladder of Perccottus glenii Dybowski, 1877 (Actinoptrygii: Odontobutidae): Supplementary data on morphology and phylogenetic position based on 18s rDNA sequence analysis

Acta Veterinaria Hungarica ◽

10.1556/004.2018.024 ◽

2018 ◽

Vol 66 (2) ◽

pp. 258-268

Author(s):

Sergey Sokolov ◽

Daria Lebedeva

Keyword(s):

Sequence Analysis ◽

18S Rdna ◽

Rdna Sequence ◽

Phylogenetic Position ◽

Amur Sleeper ◽

Molecular Characterisation ◽

Perccottus Glenii ◽

Supplementary Data ◽

18S Rdna Sequence ◽

Partial 18S

This paper is the first report on the molecular characterisation of myxozoan parasites from the odontobutid fish Chinese (Amur) sleeper (Perccottus glenii Dybowski, 1877). The authors determined the partial 18S rDNA sequence of Myxidium shedkoae Sokolov, 2013 from the gallbladder of the fish. Phylogenies reconstructed using maximum likelihood and Bayesian inference analysis revealed that M. shedkoae belongs to the hepatic biliary group of myxozoans (after Kristmundsson and Freeman, 2013) as a member of the clade consisting of Zschokkella sp. KLT-2014, Myxidium truttae and Zschokkella nova. Some new morphological features of the parasite are also presented.

Download Full-text