Radopholoides japonicus n. sp. (Nematoda: Pratylenchidae) found in rhizosphere soil associated with Podocarpus macrophyllus from Japan

Nematology ◽  
2017 ◽  
Vol 19 (9) ◽  
pp. 1095-1105
Author(s):  
Munawar Maria ◽  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Jie He ◽  
Pablo Castillo ◽  
...  
Keyword(s):  
Rhizosphere Soil ◽  
Soil Samples ◽  
Molecular Characterisation ◽  
Maximum Likelihood Analysis ◽  
Rhizospheric Soil ◽  
Long Tail ◽  
Likelihood Analysis ◽  
Lateral Lines ◽  
Podocarpus Macrophyllus ◽  
Partial 18S

Radopholoides japonicus n. sp. isolated from rhizospheric soil samples associated with Podocarpus macrophyllus imported from Japan into Ningbo Port of China is described and illustrated. It is characterised by four lateral lines, lip region low, convex with three annuli. Stylet 16.5-18.5 μm long with anteriorly sloping rounded knobs. Excretory pore at the level of pharyngo-intestinal junction. Vulva posteriorly located, vulval lips not protruding, spermatheca squarish. Post-vulval uterine sac 35-44 μm long. Tail conoid with broadly rounded terminus, phasmid 6-8 annuli posterior to anus. Among five species of genus Radopholoides, the new species is close to R. antoni but can be differentiated from it by lip and tail morphology. This is the first Radopholoides species that provided detailed morphological and molecular characterisation. Maximum Likelihood analysis using the Shimodaira-Hasegawa test for the validity of Radopholoides was performed and showed the validity of the genus using the partial 18S and D2-D3 expansion segments of 28S.

scholarly journals Molecular Study of Selected Taxonomically Critical Taxa of the Genus Iris L. from the Broader Alpine-Dinaric Area

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1229 ◽  
Author(s):  
Tim Weber ◽  
Jernej Jakše ◽  
Barbara Sladonja ◽  
Dario Hruševar ◽  
Nediljko Landeka ◽  
...  
Keyword(s):  
Maximum Likelihood ◽  
Genome Size ◽  
Molecular Study ◽  
Molecular Data ◽  
Molecular Characterisation ◽  
Maximum Likelihood Analysis ◽  
Narrow Endemic ◽  
Likelihood Analysis ◽  
Plastid Markers ◽  
Taxonomic Categories

Some wild, morphologically diverse taxa of the genus Iris in the broad Alpine-Dinaric area have never been explored molecularly, and/or have ambiguous systematic status. The main aims of our research were to perform a molecular study of critical Iris taxa from that area (especially a narrow endemic accepted species I. adriatica, for which we also analysed genome size) and to explore the contribution of eight microsatellites and highly variable chloroplast DNA (ndhJ, rpoC1) markers to the understanding of the Iris taxa taxonomy and phylogeny. Both the microsatellite-based UPGMA and plastid markers-based maximum likelihood analysis discriminated three main clusters in the set of 32 analysed samples, which correspond well to the lower taxonomic categories of the genus, and support separate status of ambiguous regional taxa (e.g., I. sibirica subsp. erirrhiza, I. x croatica and I. x rotschildii). The first molecular data on I. adriatica revealed its genome size (2C = 12.639 ± 0.202 pg) and indicated the existence of ecotypes. For future molecular characterisation of the genus we recommend the utilisation of microsatellite markers supplemented with a combination of plastid markers.

Morphological and molecular characterisation of Bursaphelenchus andrassyi sp. n. (Nematoda: Aphelenchoididae) from Romania and Turkey

Nematology ◽  
2014 ◽  
Vol 16 (2) ◽  
pp. 207-218 ◽  
Author(s):  
Mehmet Dayi ◽  
Monica Calin ◽  
Süleyman Akbulut ◽  
Jianfeng Gu ◽  
Thomas Schröder ◽  
...  
Keyword(s):  
28S Rdna ◽  
Molecular Characterisation ◽  
Species Status ◽  
Small Female ◽  
Female Tail ◽  
Lateral Lines ◽  
Conifer Wood ◽  
Partial 18S

Bursaphelenchus andrassyi sp. n., found in conifer wood samples from Romania and Turkey, is characterised morphologically and genetically. Bursaphelenchus andrassyi sp. n. clearly belongs to the sexdentati group, having a terminal bursa, four lateral lines, a very small female ‘vulval flap’, strongly arcuate spicules and the typical position of caudal papillae of males. It is morphologically most similar to B. vallesianus and B. sexdentati. It can be differentiated from B. vallesianus by the usually subcylindrical female tail with rounded or wedge-shaped terminus vs conical female tail with a more or less rounded terminus, slightly different shape of spicules (low square condylus, lacking a distinct cucullus, pointed rostrum) and from B. sexdentati by lacking a distinct post-vulval constriction, shorter stylet and shorter spicules. The species status is supported by ITS-RFLP patterns and sequencing results of both partial 18S and 28S rDNA regions.

A new record of Xiphinema dentatum Sturhan, 1978 and description of X. paradentatum sp. n. (Nematoda: Dorylaimida) from Serbia

Nematology ◽  
2017 ◽  
Vol 19 (8) ◽  
pp. 925-949 ◽  
Author(s):  
László Barsi ◽  
Elena Fanelli ◽  
Francesca De Luca
Keyword(s):  
Maximum Likelihood ◽  
New Record ◽  
Developmental Stages ◽  
The Body ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Maximum Likelihood Analysis ◽  
Likelihood Analysis ◽  
Guide Ring

A population of Xiphinema dentatum, found in a new locality in Serbia, is briefly described and illustrated. Xiphinema paradentatum sp. n. is described and illustrated. It is a parthenogenetic species characterised by its medium to moderately long body (3.71-5.02 mm), open C-shaped when heat-relaxed, lip region frontally almost flattened, laterally rounded and separated from the rest of the body by a weak depression; a moderately long odontostyle (147-166 μm), guide ring located at 137-153 μm from anterior end; two equally developed genital tracts having a pseudo-Z-organ in uterus in the form of several irregular angular sclerotised bodies – apophyses located in the vicinity of the pars dilatata uteri and devoid of spines; tail broadly and symmetrically rounded, and four juvenile developmental stages. Molecular characterisation using the ribosomal ITS and the D2-D3 expansion domains of the 28S rRNA gene was done and maximum likelihood analysis was used to reconstruct the phylogenetic relationships of X. dentatum and X. paradentatum sp. n. from Serbia with other longidorids.

Screening and antimicrobial activity of actinomycetes isolated from the rhizosphere of Clitoria ternatea

2016 ◽  
Vol 1 (01) ◽  
Author(s):  
Vemavarapu Bhaskara Rao ◽  
Kandlagunta Guru Prasad ◽  
Krishna Naragani ◽  
Vijayalakshmi Muvva
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Calcium Carbonate ◽  
Rhizosphere Soil ◽  
Soil Samples ◽  
Antimicrobial Spectrum ◽  
Clitoria Ternatea ◽  
Primary Screening

The air dried rhizosphere soil samples pretreated with calcium carbonate was employed for the isolation of actinomycete strains. Serial dilution plate technique was used for the isolation of actinomycetes. A total of 20 actinomycete strains designated as BS1-BS20 were isolated from the rhizosphere of medicinal plant Clitoria ternatea. All the 20 strains were subjected to primary screening for antimicrobial activity. Among the 20 strains screened, 10 strains exhibited high antimicrobial spectrum against Staphylococcus aureus, Escherichia coli and Candida albicans.

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

scholarly journals Trace elements in aerial parts and rhizosphere of Thymus pannonicus All.

2011 ◽  
Vol 6 (4) ◽  
pp. 616-623 ◽  
Author(s):  
Jelena Arsenijević ◽  
Slavica Ražić ◽  
Zoran Maksimović ◽  
Svetlana Đogo
Keyword(s):  
Trace Elements ◽  
Soil Acidity ◽  
Rhizosphere Soil ◽  
Absorption Spectrometry ◽  
Soil Samples ◽  
Acid Digestion ◽  
Aerial Parts ◽  
Physiological Properties ◽  
Flameless Atomic Absorption Spectrometry ◽  
Zinc Levels

AbstractThis paper brings out the results of the study on the levels of selected trace elements (Cu, Fe, Mn, Zn and Cr) in aerial parts of Thymus pannonicus All. (Lamiaceae) and rhizosphere soil from twelve locations in Serbia. Prior to assays by flame and flameless atomic absorption spectrometry, samples were subjected to microwave-assisted acid digestion. Real and potential acidity of soil samples were also measured. Obtained results for soil samples, although slightly higher for some elements (Cu: 12.38–45.18 mg/kg; Fe: 22102–46193 mg/kg; Mn: 776.95–4901.27 mg/kg; Zn: 62.27–214.02 mg/kg; Cr: 48.86–69.13 mg/kg), were found to fit into biogeochemical background. Element contents in plant samples differed depending on collecting site (Cu: 5.26–14.07 mg/kg; Fe: 25.92–1454.07 mg/kg; Mn: 89.29–278.25 mg/kg; Zn: 1.81–10.64 mg/kg; Cr: 1.11–3.51 mg/kg), which can be partly explainable by different nutrient availability influenced by soil acidity. Zinc levels in T. pannonicus were below expected and seem to be strongly influenced by plant physiological properties.

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