Description of Rotylenchus urmiaensis n. sp. (Nematoda: Hoplolaimidae) from North-western Iran with a molecular phylogeny of the genus

Nematology ◽  
2015 ◽  
Vol 17 (5) ◽  
pp. 607-619 ◽  
Author(s):  
Elmira Noruzi ◽  
Ramezan Asghari ◽  
Mohammad Reza Atighi ◽  
Ali Eskandari ◽  
Carolina Cantalapiedra-Navarrete ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Species Differentiation ◽  
Bisexual Species ◽  
Lateral Field ◽  
Western Iran ◽  
Female Tail ◽  
Longitudinal Striation ◽  
Tail Shape ◽  
Stylet Length ◽  
North Western

A new bisexual species of Rotylenchus from North-western Iran is described and illustrated based on morphological, morphometric and molecular studies. Rotylenchus urmiaensis n. sp. is characterised by having a truncate lip region with irregular longitudinal striation, lateral field areolated only in pharynx region, stylet length 34-40 μm, vulva positioned at 53-61%, and female tail conoid-rounded to dorsally convex-conoid with 5-10 annuli. Rotylenchus urmiaensis n. sp. appears to be similar to R. striaticeps, from which it may be differentiated morphologically by a slightly shorter body length (870-1269 vs 1000-1723 μm), shorter stylet (34-40 vs 39-50 μm), female tail shape (conoid-rounded to dorsally convex-conoid vs rounded), frequency of males (rare vs common as abundant as females), shorter spicules (39-43 vs 41-50 μm) and phasmid position (varying from three annuli anterior or three annuli posterior to anus vs at level to seven annuli anterior to anus), and molecularly. The results of phylogenetic analyses based on sequences of D2-D3 expansion region of 28S, ITS-rDNA, 18S rDNA, and the partial cytochrome c oxidase subunit 1 (coxI) mtDNA, confirmed the species differentiation and the close molecular relationship between R. urmiaensis n. sp. and R. striaticeps.

Description of Uliginotylenchus changlingensis n. sp. (Tylenchida: Belonolaimidae) from potato in China

Nematology ◽  
2011 ◽  
Vol 13 (1) ◽  
pp. 45-49
Author(s):  
Hui Xie ◽  
Yongsan Zeng ◽  
Chunling Xu ◽  
Chun Chen ◽  
Chunna Zhou
Keyword(s):  
Jilin Province ◽  
Lateral Field ◽  
Female Tail ◽  
Tail Shape ◽  
Stylet Length

AbstractUliginotylenchus changlingensis n. sp. is described from the rhizosphere of potato roots in Changling county, Jilin province, China. The species is characterised by a long body (female: 846-952 μm, male: 785-934 μm), completely areolated lateral field with three incisures, high and rounded offset lip region bearing five or six annuli, long stylet (female: 24.0-26.0 μm, male: 23-28 μm), presence of vulval flaps, female tail subconoid with 28-39 annuli and terminus bluntly conoid without striations, distinct phasmids and gubernaculum distally crescent-shaped. It is closely related to U. cylindricaudatus and U. bifasciatus. It differs from U. cylindricaudatus mainly by the different number of labial annuli (5-6 vs 7-8), different tail shape (subconoid vs subcylindrical) and by vulval flaps present vs absent. It differs from U. bifasciatus mainly by different female stylet length (24-26 vs 19-20 μm), tail shape and annuli (subconoid with 28-39 annuli with smooth terminus vs subcylindrical with 42-48 annuli with annulated terminus). It further differs from other species of Uliginotylenchus by the offset vs continuous labial region.

Aphelenchoides iranicus n. sp. (Nematoda: Aphelenchoididae) from West Azerbaijan province, Iran

Nematology ◽  
2016 ◽  
Vol 18 (8) ◽  
pp. 973-985 ◽  
Author(s):  
Behrouz Golhasan ◽  
Ramin Heydari ◽  
Sergio Álvarez-Ortega ◽  
Mehrab Esmaeili ◽  
Pablo Castillo ◽  
...  
Keyword(s):  
New Species ◽  
Rrna Genes ◽  
Body Contour ◽  
Lateral Field ◽  
Female Tail ◽  
Oak Tree ◽  
Stylet Length ◽  
West Azerbaijan ◽  
18S Rrna Genes ◽  
North Western

Aphelenchoidesiranicusn. sp. is described and illustrated from bark samples of an oak tree (Quercussp.) in West Azerbaijan province, north-western Iran. The new species is characterised by body length of 350 (330-383) μm in females and 343 (323-370) μm in males, lip region set off from body contour, lateral fields with three lines, stylet 8 (7-9) μm long with small basal swellings, excretory pore 59 (56-62) μm from anterior end, hemizonid invisible, pharyngeal glands overlapping intestine dorsally and extending for 48 (41-54) μm, female tail subcylindrical with many tiny finger-like appendages at the tip, and male with aphelenchoid type spicules 17.6 (17-18) μm long. The new species comes close to nine known species of the genus namely:A. brevicaudatus,A. cibolensis,A. conimucronatus,A. editocaputis,A. montanus,A. pusillus,A. rarus,A. sphaerocephalusandA. vaughani, but differs from them by the number of incisures in the lateral field, shape of tail mucron, stylet length, size of spicules and length of post-vulval uterine sac. Additionally, this species is characterised molecularly and phylogenetically by sequences of the D2-D3 expansion segments of the 28S and partial 18S rRNA genes that are unique in relation to the sequences deposited in GenBank.

Redescription of Ektaphelenchoides compsi Baujard, 1984 (Tylenchina: Aphelenchoididae) isolated from Pinus massoniana in Fujian Province, China

Nematology ◽  
2021 ◽  
pp. 1-11
Author(s):  
Hongmei Li ◽  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Xinxin Ma ◽  
Maria Munawar
Keyword(s):  
Phylogenetic Analyses ◽  
Posterior Part ◽  
Pinus Massoniana ◽  
Distal Region ◽  
Nerve Ring ◽  
Rrna Genes ◽  
Female Gonad ◽  
Fujian Province ◽  
Lateral Field ◽  
Female Tail

Summary Ektaphelenchoides compsi is redescribed morphologically with new molecular characterisation. It was isolated from a dead Pinus massoniana tree in Ningde City, Fujian Province, China. Detailed morphology of the spicule, female gonad, hemizonid position, arrangement of male caudal papillae and female tail terminus shape are documented. It is characterised by a lateral field with three lines (forming two bands), tripartite stylet 17.8 (17.0-19.4) μm long without basal thickenings, metacorpus rectangular with anterior 40% granular and posterior part weakly muscular, metacorpal valve slightly posterior to middle of metacorpus, excretory pore at level of nerve ring, vagina with thickened walls and strongly developed muscular bundles, vulval lips slightly protuberant, vulval flap absent, distal region of post-vulval uterine sac appearing as a weakly developed oogonia, anus and rectum indistinct, female posterior part (‘tail’) dorsally convex, conical, terminal region contracted into a bluntly pointed tip. The spicules are arcuate, 15.6 (14.3-16.3) μm along the chord, lamina smoothly curved to distal end, capitulum slightly concave, condylus well-developed with broadly rounded tip and slightly depressed at dorsal end, rostrum triangular with finely rounded tip, cucullus absent, and with seven caudal papillae present. The near full length 18S and 28S D2-D3 regions of rRNA genes sequences were characterised. The phylogenetic analyses revealed that the Fujian population of E. compsi grouped with the Zhejiang population of E. compsi, both being morphologically identical.

Phylogeny and redescription of Laimaphelenchus pannocaudus Massey, 1966 (Rhabditida: Aphelenchoididae) intercepted from Canadian Picea glauca

Nematology ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 199-212
Author(s):  
Yiwu Fang ◽  
Munawar Maria ◽  
Alcides Sánchez-Monge ◽  
Jianfeng Gu
Keyword(s):  
Electron Microscopy ◽  
Picea Glauca ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
The Body ◽  
Sister Relationship ◽  
Lateral Field ◽  
Female Tail ◽  
Partial 18S ◽  
Scanning Electron

Summary Laimaphelenchus pannocaudus, isolated in Ningbo port from Picea gluaca, is redescribed based upon characteristic morphological details elucidated by light and scanning electron microscopy photographs. The recovered population is characterised by 907 (771-1024) μm long females with 11.2 (8.9-12.2) μm long stylet, four incisures in the lateral field, excretory pore located at the same level as, or slightly anterior to the nerve ring, vulva lacking a flap and located at 68.7 (63.9-73.4)% of the body, elongate post-vulval uterine sac 78.5 (55.6-101.1) μm long, and tail 40.6 (33.4-45.6) μm long. Males are common and characterised by 16.5 (15.7-17.8) μm long spicules (chord) with their condylus slightly recurved, squared to rounded in shape with a blunt rounded tip and rostrum triangular with a bluntly pointed tip, and six visible caudal papillae. The female tail is ventrally curved, conoid and bears a stalk-like terminus with 4-8 pedunculate projections. Phylogenetic analyses using partial 18S and 28S rDNA D2-D3 data revealed that L. pannocaudus formed a sister relationship with L. suberensis in both phylogenies.

Rotylenchus castilloi n. sp. (Nematoda: Hoplolaimidae), a new species with long stylet from northern Iran

Zootaxa ◽  
2015 ◽  
Vol 3931 (1) ◽  
pp. 88 ◽  
Author(s):  
ATEFEH TALEZARI ◽  
EBRAHIM POURJAM ◽  
AHMAD KHEIRI ◽  
GRACIA LIÉBANAS ◽  
FARZAD ALIRAMAJI ◽  
...  
Keyword(s):  
New Species ◽  
Body Length ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Rrna Genes ◽  
Bisexual Species ◽  
Northern Iran ◽  
Stylet Length ◽  
A New Species ◽  
Specific Sequences

Rotylenchus castilloi n. sp., a new bisexual species is described and illustrated based on morphological, morphometric and molecular data. The new species is characterised by having a hemispherical, continuous lip region with an irregular corncob-like appearance under SEM, very long stylet (62–68 µm), vulva located at 49.7–62.2% of body length from anterior end, with a protruding double epiptygma, a rounded to convex-conoid (rarely bi-lobed) tail with 8–12 annuli and specific sequences of D2-D3 segments of 28S and ITS1-rRNA genes. Differences between the new species and four other species of the genus (R. mesorobustus, R. cazorlaensis, R. magnus and R. jaeni) are discussed. Morphologically, the new species can be separated from these species mostly by its body length, lip region characters, stylet length and location of phasmid. Phylogenetic analyses using 721 bp partial sequences of D2-D3 expansion segments of the 28S and 590 bp ITS1-rRNA genes revealed the new species forming a clade with two isolates of R. eximius and two isolates of R. unisexus, two morphologically unrelated species. 

Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan

Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 621-630 ◽  
Author(s):  
Yiwu Fang ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Morphological Status ◽  
Group 3 ◽  
A New Species

Aphelenchoides stellatusn. sp. is described and figured. The new species was isolated from packaging wood from Japan imported to Ningbo harbour, China. The new species has a body length of 485-533 μm (males) and 547-699 μm (females). The cuticle is weakly annulated and there are four lines in the lateral field. The stylet is 9-11 μm long and has small basal swellings. The excretory pore is located posterior to the nerve ring. Spicules smoothly curved, rose-thorn shaped. Apex and rostrum round, only slightly offset, dorsal limb 19-21 μm long. Male tail bearing six (2 + 2 + 2) caudal papillae. The female spermatheca is axial and oblong with round sperm present in multiple rows. Both male and female tail pegs have 3-4 processes, appearing star-like under SEM. The new species belongs to the Group 3 category ofAphelenchoidesspecies. Phylogenetic analyses based on full length ITS and 28S D2/D3 region of rDNA confirmed its morphological status as a new species.

Morphological and molecular characterisation of Coslenchus persicus n. sp. (Nematoda: Tylenchidae) from Iran

Nematology ◽  
2020 ◽  
pp. 1-10
Author(s):  
Manouchehr Hosseinvand ◽  
Ali Eskandari ◽  
Reza Ghaderi
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Ribosomal Genes ◽  
28S Rrna ◽  
Lateral Field ◽  
Rrna Tree ◽  
Basal Bulb ◽  
Stylet Length ◽  
North Western ◽  
A New Species

Summary A new species of Coslenchus from north-western Iran is illustrated and described using morphological, morphometric and molecular data. Coslenchus persicus n. sp. is characterised by a medium-sized body of 694-810 μm, often 18 (seldom 20-22) longitudinal ridges excluding lateral field, head with four distinct annuli, stylet 9.6-10.8 μm long, pharyngeal basal bulb large and cylindrical, spermatheca offset and ovate, without sperm, vulval flaps extending for two annuli, 5-7 μm in length, tail striated with finely to bluntly rounded tip, 110-153 μm long and without males. The new species comes close to C. assamensis, C. diversus, C. japonicus, C. leiocephalus, C. maritus, C. multigyrus, C. paramaritus, C. pastor, and C. polonicus, but can be distinguished from all by differences in body length, stylet length and shape of basal bulb, post-vulval uterine sac, tail and certain morphometric data. Molecular data are provided for the characterisation of the new species using ribosomal genes (18S and 28S rRNA). In the 18S rRNA tree, the new species was placed in a clade including one isolate of Coslenchus sp. from Iran. In the reconstructed 28S rRNA phylogenetic tree, C. persicus n. sp. clustered with other known Coslenchus species.

Description of Aphelenchoides roulingae n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from Xylocopa collaris sauteri collected from Yilan, Taiwan, with some notes on A. xylocopae Kanzaki, 2006

Nematology ◽  
2021 ◽  
pp. 1-15
Author(s):  
Natsumi Kanzaki ◽  
Hou-Feng Li ◽  
Taisuke Ekino ◽  
Yuko Takeuchi-Kaneko ◽  
Noritoshi Maehara ◽  
...  
Keyword(s):  
New Species ◽  
The Other ◽  
Lateral Field ◽  
Taxonomic Description ◽  
Female Tail ◽  
Tail Shape ◽  
Tail Tip ◽  
Associated Species

Summary Aphelenchoides roulingae n. sp. is described and illustrated. This new species was isolated from Xylocopa collaris sauteri collected at Fushan, Yilan county, Taiwan. Typologically, the new species is characterised by a three-lined lateral field, conical female tail with various and not pointed termini, male spicule with well-developed and slightly dorsally truncate condylus, blunt triangular-shaped rostrum and unevenly ventrally curved dorsal contour of the spicule blade (calomus-lamina complex). The combination of typological characters of the new species is unique. Aphelenchoides roulingae n. sp. shares the three-lined lateral field and female tail shape with ‘A. helicus’, which was previously transferred to Robustodorus from Aphelenchoides, but can be differentiated from that species by its longer and more slender female tail, narrower female tail tip, and longer female stylet. In addition, the spicule morphology of A. roulingae n. sp. is unique and not similar to that of any other Aphelenchoides species. Phylogenetically, the new species forms a well-supported clade with the other Xylocopa bee-associated species, A. xylocopae, but can be typologically and molecularly distinguished from the species. In addition to the taxonomic description, some typological characters of the genus Aphelenchoides are discussed.

Paratylenchus jasmineae sp. n. (Nematoda: Paratylenchinae) from rhizosphere of Jasminum sambac in India

Nematology ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. 469-478 ◽  
Author(s):  
Victor Phani ◽  
Vishal Singh Somvanshi ◽  
Uma Rao ◽  
Matiyar Rahaman Khan
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
28S Rrna ◽  
Lateral Field ◽  
Lateral Lines ◽  
Stylet Length ◽  
Jasminum Sambac ◽  
A New Species

Summary A new species of pin nematode, Paratylenchus jasmineae sp. n., was isolated and described from the rhizosphere of jasmine in India. The new species can be diagnosed by having a relatively larger body length of 248-355 μm, stylet length of 24.6-28.5 μm, lateral field with four lateral lines, the presence of small post-vulval uterine sac and absence of advulval flaps in the females. Additional morphological characters included continuous lip region with small projected submedian lobes, large and oblong-shaped spermatheca, the presence of prevulval swelling, and arcuate conoid tail with finely rounded terminus. Molecular characterisation of the species was carried out based on internal transcribed spacer sequence (ITS rRNA) and D2-D3 expansion segment of 28S rRNA subunit. Phylogenetic analyses based on ITS and D2-D3 molecular markers resulted in clear separation of P. jasmineae sp. n. from other Paratylenchus species.

Phylogenetic relationships of Cacopaurus pestis Thorne, 1943 within representatives of the Tylenchulidae Skarbilovich, 1947 as inferred from ITS and D2-D3 expansion segments of 28S-rRNA sequences

Nematology ◽  
2019 ◽  
Vol 21 (9) ◽  
pp. 971-994 ◽  
Author(s):  
Abas Mokaram Hesar ◽  
Akbar Karegar ◽  
Reza Ghaderi
Keyword(s):  
Plant Species ◽  
Phylogenetic Relationships ◽  
Phylogenetic Trees ◽  
Phylogenetic Analyses ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Stylet Length ◽  
Ectoparasitic Nematodes ◽  
North Western ◽  
Rrna Sequences

Summary The Persian sessile nematode (Cacopaurus pestis) and pin nematodes (Paratylenchus spp.) are sedentary ectoparasitic nematodes associated with many plant species worldwide. In this study, we provide morphological and molecular characterisation of seven populations of C. pestis and eight species of Paratylenchus recovered from north-western Iran. A total of 26 new sequences of D2-D3 expansion segments of 28S rRNA and ITS rRNA were obtained and used to reconstruct phylogenetic trees. Results of phylogenetic analyses revealed that the subfamilies of Tylenchulidae form well-separated clades, but that the genera Cacopaurus and Paratylenchus (= Gracilacus) in the subfamily Paratylenchinae are clustered in one clade. It appears that the previously used character of “stylet length greater than 40 μm” is not homologous and evolved more than once within the Paratylenchinae.

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