Phylogeny and redescription of Laimaphelenchus pannocaudus Massey, 1966 (Rhabditida: Aphelenchoididae) intercepted from Canadian Picea glauca

Nematology ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 199-212
Author(s):  
Yiwu Fang ◽  
Munawar Maria ◽  
Alcides Sánchez-Monge ◽  
Jianfeng Gu
Keyword(s):  
Electron Microscopy ◽  
Picea Glauca ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
The Body ◽  
Sister Relationship ◽  
Lateral Field ◽  
Female Tail ◽  
Partial 18S ◽  
Scanning Electron

Summary Laimaphelenchus pannocaudus, isolated in Ningbo port from Picea gluaca, is redescribed based upon characteristic morphological details elucidated by light and scanning electron microscopy photographs. The recovered population is characterised by 907 (771-1024) μm long females with 11.2 (8.9-12.2) μm long stylet, four incisures in the lateral field, excretory pore located at the same level as, or slightly anterior to the nerve ring, vulva lacking a flap and located at 68.7 (63.9-73.4)% of the body, elongate post-vulval uterine sac 78.5 (55.6-101.1) μm long, and tail 40.6 (33.4-45.6) μm long. Males are common and characterised by 16.5 (15.7-17.8) μm long spicules (chord) with their condylus slightly recurved, squared to rounded in shape with a blunt rounded tip and rostrum triangular with a bluntly pointed tip, and six visible caudal papillae. The female tail is ventrally curved, conoid and bears a stalk-like terminus with 4-8 pedunculate projections. Phylogenetic analyses using partial 18S and 28S rDNA D2-D3 data revealed that L. pannocaudus formed a sister relationship with L. suberensis in both phylogenies.

Redescription of Ektaphelenchoides compsi Baujard, 1984 (Tylenchina: Aphelenchoididae) isolated from Pinus massoniana in Fujian Province, China

Nematology ◽  
2021 ◽  
pp. 1-11
Author(s):  
Hongmei Li ◽  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Xinxin Ma ◽  
Maria Munawar
Keyword(s):  
Phylogenetic Analyses ◽  
Posterior Part ◽  
Pinus Massoniana ◽  
Distal Region ◽  
Nerve Ring ◽  
Rrna Genes ◽  
Female Gonad ◽  
Fujian Province ◽  
Lateral Field ◽  
Female Tail

Summary Ektaphelenchoides compsi is redescribed morphologically with new molecular characterisation. It was isolated from a dead Pinus massoniana tree in Ningde City, Fujian Province, China. Detailed morphology of the spicule, female gonad, hemizonid position, arrangement of male caudal papillae and female tail terminus shape are documented. It is characterised by a lateral field with three lines (forming two bands), tripartite stylet 17.8 (17.0-19.4) μm long without basal thickenings, metacorpus rectangular with anterior 40% granular and posterior part weakly muscular, metacorpal valve slightly posterior to middle of metacorpus, excretory pore at level of nerve ring, vagina with thickened walls and strongly developed muscular bundles, vulval lips slightly protuberant, vulval flap absent, distal region of post-vulval uterine sac appearing as a weakly developed oogonia, anus and rectum indistinct, female posterior part (‘tail’) dorsally convex, conical, terminal region contracted into a bluntly pointed tip. The spicules are arcuate, 15.6 (14.3-16.3) μm along the chord, lamina smoothly curved to distal end, capitulum slightly concave, condylus well-developed with broadly rounded tip and slightly depressed at dorsal end, rostrum triangular with finely rounded tip, cucullus absent, and with seven caudal papillae present. The near full length 18S and 28S D2-D3 regions of rRNA genes sequences were characterised. The phylogenetic analyses revealed that the Fujian population of E. compsi grouped with the Zhejiang population of E. compsi, both being morphologically identical.

Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan

Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 621-630 ◽  
Author(s):  
Yiwu Fang ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Morphological Status ◽  
Group 3 ◽  
A New Species

Aphelenchoides stellatusn. sp. is described and figured. The new species was isolated from packaging wood from Japan imported to Ningbo harbour, China. The new species has a body length of 485-533 μm (males) and 547-699 μm (females). The cuticle is weakly annulated and there are four lines in the lateral field. The stylet is 9-11 μm long and has small basal swellings. The excretory pore is located posterior to the nerve ring. Spicules smoothly curved, rose-thorn shaped. Apex and rostrum round, only slightly offset, dorsal limb 19-21 μm long. Male tail bearing six (2 + 2 + 2) caudal papillae. The female spermatheca is axial and oblong with round sperm present in multiple rows. Both male and female tail pegs have 3-4 processes, appearing star-like under SEM. The new species belongs to the Group 3 category ofAphelenchoidesspecies. Phylogenetic analyses based on full length ITS and 28S D2/D3 region of rDNA confirmed its morphological status as a new species.

Preparation of Alcohol-Preserved Larvae of Culicidae (Diptera) for Scanning Electron Microscopy

1972 ◽  
Vol 3 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Christine Dahl
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
The Body ◽  
Taxonomic Studies ◽  
Scanning Electron ◽  
Sem Studies

AbstractA method for preparation of alcohol-preserved culicid larvae for Scanning Electron Microscope (SEM) studies is described. It is based on dehydration by ethanol-xylol and fast evaporation of xylol in +8o° C. for ten minutes. For taxonomic studies such as examination of pecten teeth, comb scales and microtrichiae in magnifications up to 6oooX the method is suitable. For studies of receptor structures on hair-tufts and microstructures of the body integument alcohol preserved material is less satisfactory. The microstructure of the comb scales is figured and their function discussed. Differences in the ultrastructure of the abdominal hair-tufts are pointed out.

Phylogenetic and morphological analyses of species of the Entolomataceae (Agaricales, Basidiomycota) with cuboid basidiospores

Phytotaxa ◽  
2019 ◽  
Vol 391 (1) ◽  
pp. 1
Author(s):  
FERNANDA KARSTEDT ◽  
MARINA CAPELARI ◽  
TIMOTHY J. BARONI ◽  
DAVID L. LARGENT ◽  
SARAH E. BERGEMANN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analyses ◽  
Profile View ◽  
Pileus Surface ◽  
Scanning Electron

The generic or subgeneric delimitation by morphology of the Entolomataceae (Agaricales, Basidiomycota) is often based on the habit and external features of the basidiomata, the hyphal arrangement of the pileus surface and the shape of the basidiospores, which possess either bumps or undulate-pustules forming short ridges, or longitudinal ridges or are obviously angular with four to nine angles in profile view. Here, we examine the basidiospore shape of species in the /Entoloma clade described as cuboid to evaluate its importance in taxonomy using both phylogenetic and detailed analyses of the shape with Scanning Electron Microscopy. Our phylogenetic analyses support the placement of species with cuboid basidiospores into one of two clades. Based on this separation, two new subgenera of Entoloma are proposed: Cuboeccilia with an omphalinoid habit and fusoid cystidia and Cubospora which has a mycenoid to tricholomatoid habit and clavate, rarely fusoid cheilocystidia.

scholarly journals Congenital infection with Setaria digitata and Setaria marshalli in the thoracic cavity of a Korean calf: a case report

2010 ◽  
Vol 55 (No. 6) ◽  
pp. 275-280 ◽  
Author(s):  
NS Kim ◽  
HC Kim ◽  
C. Sim ◽  
JR Ji ◽  
NS Kim ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Case Report ◽  
Congenital Infection ◽  
The Body ◽  
Thoracic Cavity ◽  
Setaria Digitata ◽  
Scanning Electron

In March 2010, a 3.5-month-old Korean native calf was anatomized and two nematode worms were detected in the thoracic cavity. The worms were identified and classified by light and scanning electron microscopy on the basis of features at the anterior and posterior parts of the worms. The worms were female Setaria digitata and Setaria marshalli and numerous eggs which contained microfilaria were detected in the uterus of both species. The body lengths of the S. digitata and S. marshalli were 78 mm and 117 mm, respectively. Mosquitoes act as the vector for Setaria nematodes but these are inactivated in winter in Korea. Therefore, we concluded that this case represented setariasis with congenital infection occurring during the summer prenatal stage of life.

scholarly journals Speciation in the genera Anthericum and Chlorophytum (Asparagaceae) in Ethiopia—a molecular phylogenetic approach

Phytotaxa ◽  
2017 ◽  
Vol 297 (2) ◽  
pp. 139 ◽  
Author(s):  
Charlotte Sletten Bjorå ◽  
MARTE ELDEN ◽  
INGER NORDAL ◽  
ANNE K. BRYSTING ◽  
TESFAYE AWAS ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
Molecular Data ◽  
Group Relations ◽  
Variation Patterns ◽  
Molecular Phylogenetic ◽  
Lowland Area ◽  
A New Species ◽  
Scanning Electron

Sister group relations of Ethiopian species of Anthericum and Chlorophytum and variation patterns in the C. gallabatense and C. comosum complexes were studied using molecular phylogenetic analyses, morphometrics, and scanning electron microscopy of seed surfaces. Results indicate that molecular data largely support previous morphological conclusions, and that speciation has occurred in Ethiopia at least three times in Anthericum and repeatedly within different subclades of Chlorophytum. Areas particularly rich in endemic species are the lowland area around Bale Mountains in SE Ethiopia and in the Beninshangul Gumuz regional state in W Ethiopia near the border to Sudan. A new species, Chlorophytum mamillatum Elden & Nordal, is described, and the names C. tordense and C. tetraphyllum are re-instated.

scholarly journals Balantidium grimi n. sp. (Ciliophora, Litostomatea), a new species inhabiting the rectum of the frog Quasipaa spinosa from Lishui, China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 29 ◽  
Author(s):  
Weishan Zhao ◽  
Can Li ◽  
Dong Zhang ◽  
Runqiu Wang ◽  
Yingzhen Zheng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
New Species ◽  
Body Shape ◽  
Ssu Rdna ◽  
The Body ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Scanning Electron

Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is “V”-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.

Morphology of the lateral fields of the infective juvenile of the entomopathogenic family Steinernematidae (Nematoda) using high pressure freezing (HPF)

Nematology ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 69-74
Author(s):  
Zdeněk Mráček ◽  
Jiří Nermut’ ◽  
Martina Tesařová ◽  
Vladimír Půža
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Pressure ◽  
Infective Juvenile ◽  
Field Pattern ◽  
Taxonomic Character ◽  
High Pressure Freezing ◽  
Lateral Field ◽  
Transmission Electron ◽  
Scanning Electron

Summary The lateral field pattern of infective juveniles of the nematode family Steinernematidae is an important taxonomic character. Scanning electron microscopy (SEM) shows the number of ridges and lines or incisures clearly, but does not provide other details. In the present study, ten species from six clades of Steinernematidae have been studied for their lateral field morphology using SEM and high pressure freezing (HPF) with transmission electron microscopy (TEM). Both methods indicated the same number of ridges and lines, although HPF/TEM resulted in a more detailed morphology with differences between the species. The tips of the ridges are either finely rounded or pointed and the lines are V-shaped or have a broadened bottom. These characters represent an additional pattern that may be characteristic for some species within the phylogenetic clades. Further studies of the lateral field morphology of other species is needed to ascertain whether each pattern is clade specific and phylogenetically valuable.

Composition and Microstructure of a Natural Nano-Structure Mineral: Six-Ring Rock

2011 ◽  
Vol 295-297 ◽  
pp. 869-872
Author(s):  
Qing Shan Li ◽  
Xin Wang ◽  
Jun Liu ◽  
Guang Zhong Xing
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Energy Dispersive Spectrometer ◽  
The Body ◽  
X Ray Diffraction ◽  
Nano Structure ◽  
X Ray ◽  
Transmission Electron ◽  
Nano Size ◽  
Scanning Electron

Six-ring Rock is widely used as containers of water and additives to produce health care products. In this paper, the composition and microstructure of Six-ring Rock have been investigated by using scanning electron microscopy, energy dispersive spectrometer, transmission electron microscopy, x-ray diffraction and other technologies. Results show that Six-ring Rock is composed of CaMg(CO3)2, SiO2 and KAlSi3O8. Fe atoms exist in CaMg(CO3)2 by replacing Mg atoms. Six-ring Rock shows nano-size lamellar and acerose microstructures on the surface, and nano-size monocrystals in the body. Six-ring Rock is a natural nano structure mineral.

Scanning electron microscopy of the cuticular armature of the nematode Gnathostoma spinigerum Owen, 1836 from cats in Laos

1990 ◽  
Vol 64 (3) ◽  
pp. 255-262 ◽  
Author(s):  
Tomáš Scholz ◽  
Oleg Ditrich
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Spatial Arrangement ◽  
Felis Catus ◽  
The Body ◽  
Immature Female ◽  
Gnathostoma Spinigerum ◽  
First Time ◽  
Scanning Electron

ABSTRACTThe body surface of an immature female Gnathostoma spinigerum found for the first time in the definitive host (Felis catus f. domestica) in Laos was studied using a scanning electron microscope. All types of cuticular spines, which are one of the most important features for species identification of gnathostomid nematodes, together with their spatial arrangement, are described and figured.

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