Molecular phylogenetic study of Bealius Massey & Hinds, 1970 (Nematoda: Hexatylina: Sphaerulariidae), with description of B. pinus n. sp. from Iran

Nematology ◽  
2019 ◽  
Vol 21 (4) ◽  
pp. 435-444
Author(s):  
Mehrab Esmaeili ◽  
Ramin Heydari ◽  
Behrouz Golhasan ◽  
Weimin Ye
Keyword(s):  
New Species ◽  
Nematode Species ◽  
Small Subunit ◽  
Phylogenetic Study ◽  
Pharyngeal Bulb ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Posterior Extension ◽  
Stylet Length

Summary Bealius pinus n. sp. is described and illustrated based on its morphological, morphometric, and molecular characters. The new species is characterised by a body length of 1040 (920-1124) μm (females) and 985 (990-1043) μm (males), lip region continuous, 7.2 (7.0-8.0) μm broad, 3.3 (3.0-4.0) μm high, stylet length 8.4 (7.0-9.0) μm or ca 1.0-1.3 times the lip region diam., lateral fields with five or six incisures, the outer incisures crenate and inner incisures weakly crenate, and excretory pore situated 156 (141-170) μm from the anterior end. The basal pharyngeal bulb has a short posterior extension projecting dorsally into the intestine. Post-uterine sac absent. Functional males common in the population, spicules 29 (28-30) μm long. Tail cylindrical with broadly rounded terminus in the female and conical, arcuate, completely enveloped by a well-developed bursa in the male. The new species is compared with two known species of the genus, B. bisulcus and B. pissodi. Molecular phylogenetic studies of the new species using partial sequences of small subunit (SSU) rDNA revealed that it forms a clade with several nematode species belonging to genera in the Sphaerularioidea, namely: Sphaerularia, Paurodontella, Nothotylenchus, and Deladenus.

Description of Ditylenchus acantholimonis n. sp. (Rhabditida: Anguinidae) from Iran, a morphological and molecular phylogenetic study

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Samira Aliverdi ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Rhizosphere Soil ◽  
Phylogenetic Analyses ◽  
Phylogenetic Study ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Golestan Province ◽  
Tail Tip

Summary Ditylenchus acantholimonis n. sp. is described based on morphological, morphometric and molecular characters. It was isolated from the rhizosphere soil of Acantholimon sp. in Golestan province, Iran, and is mainly characterised by having four lines in the lateral field, a pyriform to bottle-shaped offset pharyngeal bulb, post-vulval uterine sac 36.6-56.1% of the vulva to anus distance long, and a subcylindrical to conical tail with widely rounded tip. It is further characterised by short to medium-sized females, 480-617 μm long, with a fine stylet having small rounded knobs, V = 80.8-83.6, c = 11.0-13.8, c′ = 3.3-4.6, and males with 16.0-17.0 μm long spicules. The new species was morphologically compared with six species having four lines in their lateral field, rounded tail tip and comparable morphometric data namely: D. dipsacoideus, D. emus, D. exilis, D. paraparvus, D. sturhani, and D. solani. It was also compared with two species, D. ferepolitor and D. angustus, forming a maximally supported clade in the 18S tree. The phylogenetic analyses using the maximal number of Anguinidae and several Sphaerularioidea genera based upon partial 18S and 28S rDNA D2-D3 sequences revealed that Ditylenchus is polyphyletic. In the 18S tree, the new species formed a clade with D. ferepolitor (KJ636374) and D. angustus (AJ966483); in the 28S tree it formed a poorly supported clade with D. phyllobios (KT192618) and Ditylenchus sp. (MG865719).

Proposal of a new subfamily of Sciaridae (Diptera: Sciaridae), with description of one new species from South Korea

Zootaxa ◽  
2019 ◽  
Vol 4543 (1) ◽  
pp. 127 ◽  
Author(s):  
SEUNGGWAN SHIN ◽  
HEUNGSIK LEE ◽  
SEUNGHWAN LEE
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Phylogenetic Study ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Unidentified Species ◽  
Definition Of ◽  
One New Species ◽  
Key Characters

Here we propose a new monophyletic subfamily, the Chaetosciarinae, based on previous morphological cladistic and molecular phylogenetic studies. This new subfamily includes the genera Chaetosciara Frey, Mouffetina Frey, Schwenckfeldina Frey, and Scythropochroa Enderlein. We also provide a definition of the new subfamily Chaetosciarinae and describe common morphological key characters. Three Korean Chaetosciarinae species in three genera are reported, all of which are new to Korea and one (Scythropochroa pseudoquercicola sp. nov.) of which is new to science. A previous molecular phylogenetic study designated Scy. pseudoquercicola as an unidentified species. Our study provides examined species information for members of this new subfamily to accompany the GenBank accession numbers published by a previous molecular phylogenetic study. Furthermore, we present a multigene molecular phylogenetic analysis for the Chaetosciarinae. 

Molecular and morphological characterization of Paurodontoides siddiqii n. sp. (Nematoda: Hexatylina, Sphaerulariidae) associated with bark samples of Pinus eldarica from western Iran

2018 ◽  
Vol 94 ◽  
Author(s):  
M. Esmaeili ◽  
B. Golhasan ◽  
W. Ye ◽  
R. Heydari
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Morphological Characterization ◽  
Body Contour ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Molecular Phylogenetic ◽  
Posterior Extension ◽  
Stylet Length

Abstract A new species of Paurodontoides, P. siddiqii n. sp., is described and illustrated based on its morphological, morphometric, and molecular characters. The new species is characterized by a female 550–729 μm long, lip region continuous with body contour, stylet length 7.0–8.0 μm long or c. 1.0–1.2 times the lip region diameter, lateral fields with four smooth incisures, excretory pore at 85–125 μm from anterior end located at the base of the pharyngeal bulb or posterior to it, basal pharyngeal bulb with a short posterior extension projecting into the intestine, monodelphic–prodelphic reproductive system with prominent 19–22 μm long post-uterine sac, and elongate conoid tail with a filiform terminus. The new species is compared with two known species of the genus. It differs from the type species of the genus, P. linfordi, by having slightly shorter stylet, lateral field with smooth incisures, different position of the excretory pore, and absence of male. Compared to P. latus, the new species has a shorter body, shorter stylet, different position of the excretory pore, female tail shape and absence of male. The new species was also compared with close species of the genus Paurodontus because of lateral field marked with four lines, asymmetrical stylet knobs and absence of male. Molecular phylogenetic studies of the new species using partial sequences of 18S rDNA revealed that it forms a clade with a species of the genus Ficotylus. In phylogenetic analyses using partial sequences of the 28S rDNA D2-D3 domain, the new species formed a monophyletic group with a species of the genus Veleshkinema and Sphaerularia spp. (Sphaerulariinae).

Observations on Malenchus geraerti n. sp. (Rhabditida: Tylenchidae), a morphological and molecular phylogenetic study

Zootaxa ◽  
2018 ◽  
Vol 4369 (3) ◽  
pp. 406 ◽  
Author(s):  
MAJID PEDRAM ◽  
MAHYA SOLEYMANZADEH ◽  
EBRAHIM POURJAM ◽  
MAHYAR MOBASSERI
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Phylogenetic Study ◽  
Molecular Characteristics ◽  
Lsu Rdna ◽  
Northern Iran ◽  
Lateral Field ◽  
Molecular Phylogenetic ◽  
Short Body

Malenchus geraerti n. sp., recovered from natural regions of northern Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having females with a short body, an anteriorly wide S-shaped amphidial opening narrowing posteriorly, cuticle with prominent annuli, lateral field a plain band with smooth margins, muscular metacorpus with well-developed valve and corresponding plates, spermatheca filled with small spheroid sperm cells, vulva sunken in body with large epiptygma and no flap, and conical tail tapering gradually to a more or less pointed tip. Males of the new species are characterized by having a short body, tylenchoid spicules, adcloacal bursa with smooth margin and tail similar to that of the female. Morphologically, the new species is similar to five known species of the genus: M. fusiformis, M. machadoi, M. pachycephalus, M. solovjovae and M. undulatus. It most closely resembles M. pachycephalus, but as a cryptic species it can be differentiated using morphological and molecular characteristics. Comparisons with the four other aforementioned species are also discussed. Molecular phylogenetic studies using partial sequences of small and large subunit ribosomal DNA fragments reveal that the new species forms a clade with the species M. neosulcus in the small subunit (SSU) rDNA, and two species of Lelenchus in the large subunit (LSU) rDNA tree. 

Molecular and morphological characterisation of Paurodontella persica n. sp. (Hexatylina: Sphaerulariidae) from soil in Iran

Nematology ◽  
2017 ◽  
Vol 19 (1) ◽  
pp. 57-68 ◽  
Author(s):  
Mehrab Esmaeili ◽  
Ramin Heydari ◽  
Weimin Ye
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Body Contour ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Rdna Gene ◽  
Posterior Extension ◽  
Large Subunit Rdna ◽  
A New Species

A new species ofPaurodontella,P. persican. sp., collected from the Alborz rangelands of Iran, is described and illustrated. The new species is characterised by its body length of 506 (416-599) μm and 424 (388-461) μm in female and male, respectively, four incisures in lateral field, lip region annulated and continuous with body contour, total stylet 6.2 (5.5-7.0) μm long. Basal pharyngeal bulb with small posterior extension projecting reaching to the intestine. Excretory pore situated at the level of basal pharyngeal bulb region. Post-uterine sac short, 4.5 (3.0-6.0) μm long and uterus with an offset diverticulum. Tail of both sexes similar, conoid pointed, terminus tapering into a long pointed mucron. Male with adanal bursa and spicules 16.5 (16.0-17.0) μm long. The new species comes close in morphology and morphometrics to four known species of the genus, namelyP. auriculata,P. myceliophaga,P. minutaandP. niger. Molecular analyses of the partial small subunit rDNA gene (SSU), D2-D3 expansion segments of the large subunit rDNA gene (LSU) and internal transcribed spacer (ITS) revealed this to be a new species.

scholarly journals Microcos magnifica (Sparrmanniaceae) a new species of cloudforest tree from Cameroon

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e4137 ◽  
Author(s):  
Martin Cheek
Keyword(s):  
New Species ◽  
Conservation Status ◽  
Phylogenetic Study ◽  
Herbarium Specimens ◽  
Additional Species ◽  
Systematic Revision ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Binocular Microscope ◽  
Made In

Background Although many new species to science have been discovered from thousands of specimens resulting from botanical inventories to support conservation management in Cameroon in recent years, additional species remain to be formally evaluated taxonomically and described. These include species from genera which have been taxonomically neglected for many decades in Africa, such as Microcos. Methods This study is based mainly on herbarium specimens and field observations made in Cameroon during a series of botanical surveys. Herbarium material was examined with a Leica Wild M8 dissecting binocular microscope fitted with an eyepiece graticule. Principal Findings Microcos magnifica Cheek (Malvaceae-Grewioideae or Sparrmanniaceae) is described as an Endangered (EN B2 ab(iii)) new tree species from the submontane forests of Cameroon. It is illustrated and described, and its conservation status and taxonomic affinities are assessed. It is the first new Microcos described from Africa in more than 90 years and is unique on the continent in having sculptured fruits. Discussion A systematic revision, with a molecular phylogenetic study, of Microcos Burm. ex L. in Africa is necessary if the affinities of the species, including M. magnifica, are to be reliably established.

scholarly journals A New Species of Cryptopone Emery (Hymenoptera: Formicidae: Ponerinae) from Brazil with Observations of this Genus and a Key for New World Species

Sociobiology ◽  
2019 ◽  
Vol 66 (3) ◽  
pp. 408
Author(s):  
Itanna Oliveira Fernandes ◽  
Jacques Hubert Charles Delabie
Keyword(s):  
New Species ◽  
New World ◽  
First Record ◽  
Neotropical Region ◽  
The State ◽  
Phylogenetic Study ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Large Genus ◽  
East And Southeast Asia

The monophyly of Ponerinae was strongly supported by a detailed molecular phylogenetic study. Within the subfamily, substantial changes were yet done to the taxonomy of several genera, such as Cryptopone Emery, and after phylogenetic and morphological considerations, the genus Cryptopone was revived. Cryptopone is a moderately large genus of pantropical distribution, with 25 described species and subspecies, with its diversity centered mostly in East and Southeast Asia. In the New World, only four species were known until now, Cryptopone gilva (Roger), Cryptopone guianensis (Weber), Cryptopone holmgreni (Wheeler) and Cryptopone mirabilis (Mackay & Mackay). Since the Mackay and Mackay’s revision of 2010 of the genus Pachycondyla which included the species currently attributed to Cryptopone, no new species was added to Cryptopone genus in the New World. Recently an unidentified Cryptopone species was collected in the state of Rondônia, Brazil. The material here described was sampled by manual collection of soil in the Floresta Nacional do Jamari (FLONA Jamari). Four individuals belonging to the worker caste are hereafter described under the name of Cryptopone pauli sp. nov. Currently this ant is known only to a single locality in the state of Rondônia, Brazil. This is also the first record of this genus for that state. We present new records of Cryptopone for the Neotropical Region with some comments on its biology and an updated key to workers of the five species of Cryptopone currently known in the New World.

Molecular phylogenetic study in Spirocercidae (Nematoda) with description of a new species Spirobakerus sagittalis sp. nov. in wild canid Cerdocyon thous from Brazil

2021 ◽  
Author(s):  
Ana Paula Nascimento Gomes ◽  
Michele Maria dos Santos ◽  
Natalie Olifiers ◽  
Roberto do Val Vilela ◽  
Mayara Guimarães Beltrão ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Study ◽  
Molecular Phylogenetic Study ◽  
Wild Canid ◽  
Molecular Phylogenetic ◽  
Cerdocyon Thous ◽  
A New Species
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