Anatomy of Collapse in Eucalyptus Species

IAWA Journal ◽  
1987 ◽  
Vol 8 (3) ◽  
pp. 291-295 ◽  
Author(s):  
J. Wilkes ◽  
A.P. Wilkins
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wall Thickness ◽  
Cell Types ◽  
Tangential Direction ◽  
Lumen Diameter ◽  
Wall Structure ◽  
Eucalyptus Species ◽  
Scanning Electron

Scanning electron microscopy was employed to examine the anatomy of collapse in Eucalyptus bancroftii, E. macrorhyncha, E. nitens, E. oreades, and E. pilularis. Collapse appeared to be restricted to fibres, although other cell types sometimes distorted in response to the stresses developed. The propensity for individual fibres to collapse was not always related to the ratio of wall thickness to lumen diameter, and a complex of factors, e.g. proximity to other cell types, may be involved. Collapse, which was most prevalent in the tangential direction, was rarely accompanied by detectable damage to the wall structure, suggesting that the strength of affected timber should not be seriously diminished.

scholarly journals Synthesis and Evaluation of AlgNa-g-poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone

2021 ◽  
Vol 22 (11) ◽  
pp. 5730
Author(s):  
Jomarien García-Couce ◽  
Marioly Vernhes ◽  
Nancy Bada ◽  
Lissette Agüero ◽  
Oscar Valdés ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Controlled Release ◽  
Biomedical Applications ◽  
Release Kinetics ◽  
Cell Types ◽  
Tissue Engineering Scaffolds ◽  
Almost All ◽  
Scanning Electron

Hydrogels obtained from combining different polymers are an interesting strategy for developing controlled release system platforms and tissue engineering scaffolds. In this study, the applicability of sodium alginate-g-(QCL-co-HEMA) hydrogels for these biomedical applications was evaluated. Hydrogels were synthesized by free-radical polymerization using a different concentration of the components. The hydrogels were characterized by Fourier transform-infrared spectroscopy, scanning electron microscopy, and a swelling degree. Betamethasone release as well as the in vitro cytocompatibility with chondrocytes and fibroblast cells were also evaluated. Scanning electron microscopy confirmed the porous surface morphology of the hydrogels in all cases. The swelling percent was determined at a different pH and was observed to be pH-sensitive. The controlled release behavior of betamethasone from the matrices was investigated in PBS media (pH = 7.4) and the drug was released in a controlled manner for up to 8 h. Human chondrocytes and fibroblasts were cultured on the hydrogels. The MTS assay showed that almost all hydrogels are cytocompatibles and an increase of proliferation in both cell types after one week of incubation was observed by the Live/Dead® assay. These results demonstrate that these hydrogels are attractive materials for pharmaceutical and biomedical applications due to their characteristics, their release kinetics, and biocompatibility.

Pollen Wall Structure Using a New Stripping-Sputtering Device for Scanning Electron Microscopy

Grana ◽  
1995 ◽  
Vol 34 (5) ◽  
pp. 325-331 ◽  
Author(s):  
Shengyin Lan ◽  
Zhenxiu Xu ◽  
Tingdong Fu ◽  
Waheeb K. Heneen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Wall ◽  
Wall Structure ◽  
Scanning Electron

scholarly journals Morphological and physicochemical diversity of snow algae from Alaska

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Marta J. Fiołka ◽  
Nozomu Takeuchi ◽  
Weronika Sofińska-Chmiel ◽  
Sylwia Mieszawska ◽  
Izabela Treska
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Cell Types ◽  
Microscopy Imaging ◽  
Excitation Light ◽  
X Ray ◽  
Acridine Orange Staining ◽  
Snow Algae ◽  
Scanning Electron

Abstract Snow algae are photosynthetic microbes growing in thawing snow. They usually show various morphological cell types. The aim of this study was to carry out microscopic and spectroscopic analysis of different forms of cells of snow algae collected on glaciers in Alaska. Four different shapes of algal cells were observed with the use of bright field LM (Light Microscopy), DIC (Differential Interference Contrast), EDF (Extended Depth Focus), fluorescence microscopy, and SEM (Scanning Electron Microscopy). The cells exhibited the strongest autofluorescence after the exposure to 365-nm excitation light, and the intensity differed among the cell types. Zygotes (cysts) showed the most intense fluorescence. Acridine orange staining revealed the acid nature of the algal cells. The use of Congo red and Calcofluor white fluorochromes indicated differences in the structure of polysaccharides in the cell wall in the individual types of algal cells. FTIR (Fourier-Transform Infrared Spectroscopy) analyses showed the presence of polysaccharides not only in the algal cells but also in the fixative solution. The presence of polysaccharides in the extracellular algal fraction was confirmed by X-ray dispersion spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy imaging (SEM). The differences observed in the structure of the cell wall of the different forms of red snow algae prompt further analysis of this structure.

scholarly journals Investigations on the postnatal development of the foliate papillae using light and scanning electron microscopy in the porcupine (Hystrix cristata)

2013 ◽  
Vol 58 (No. 6) ◽  
pp. 318-321 ◽  
Author(s):  
S. Yilmaz ◽  
A. Aydin ◽  
G. Dinc ◽  
B. Toprak ◽  
M. Karan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Types ◽  
Taste Buds ◽  
The Other ◽  
Average Depth ◽  
Hystrix Cristata ◽  
Basal Half ◽  
Different Cell Types ◽  
Scanning Electron

In this study SEM and light microscopy were used to investigate the structure of the foliate papillae in the porcupine. The foliate papillae consisted of about 10 or 11 clefts. The length of the foliate papillae averaged 2.79 mm and its width averaged 863 µm. Taste buds were located intraepithelial in the basal half of the papilla grooves (sulcus papillae). Every wall on each fold harboured from five to nine taste buds. There were two different cell types of taste buds: one stained light (epitheliocytus sensorius gustatorius), and the other dark (epitheliocytus sustentans). The length and width of the taste buds averaged 190.5 µm and 86 µm, respectively. The ratio of the length to the width of taste buds was 2.21. The average depth of the papilla groves was 1763 µm and its epithelial thickness was 235.5 µm. In scanning electron microscopy, the thickness of the epithelial cell borders was apparent at higher magnifications and there micro-ridges and micro-pits were apparent on the surfaces of these cells.  

Chemical and enzymatic changes in the cell walls of Candida albicans and Saccharomyces cerevisiae by scanning electron microscopy

1980 ◽  
Vol 26 (8) ◽  
pp. 965-970 ◽  
Author(s):  
Yvonne Koch ◽  
K. H. Rademacher
Keyword(s):  
Electron Microscopy ◽  
Saccharomyces Cerevisiae ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Candida Albicans ◽  
Cell Walls ◽  
Wall Structure ◽  
Before And After ◽  
Enzymatic Changes ◽  
Scanning Electron

Candida albicans and Saccharomyces cerevisiae cells were examined by scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area of the scars. Helicase digested irregular holes through the cell wall within 20 min; these increased in size during an additional 40 min of digestion. These holes were not localized in or on the bud scars, which remained intact even after the long digestion period. The results were used to construct a model for yeast cell wall structure.

scholarly journals Macroscopic and Microscopic Characteristics of 2- and 4-Year-Old Schizostachyum brachycladum

2019 ◽  
pp. 62-69
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Vascular Bundle ◽  
Middle Lamella ◽  
Cell Wall Structure ◽  
Wall Structure ◽  
Transmission Electron ◽  
Scanning Electron

Anatomical of cell wall structure on Schizostachyum brachycladum examined. The harvested two-year-old and four-year-old bamboo culms segregated into the bottom, middle and top portions. The samples then undergo the Light Microscopy (LM), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) to determine their structure such as a vascular bundle, parenchyma, and sclerenchyma. Results show the surface of bamboo was visualized by LM to decide on their structural figure. In this part, 2-year age indicated that higher numbers of vascular bundle and average of mean compared to the 4-year S. brachycladum. Followed by a specific study of cell wall structure using SEM with highlighted 4-year S. brachycladum had more complex of morphology structure compared to the 2-year-old. Later on, TEM illustrated to shows most depth anatomically structure of bamboo such as middle lamella, primary and secondary walls.

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