The Case of Fish Skin

2021 ◽  
pp. 185-199
Keyword(s):  
Fish Skin

An immunocytochemical study of maternal immunoglobulin localization in the suckling pig intestine

1990 ◽  
Vol 48 (3) ◽  
pp. 922-923
Author(s):  
László G. Kömüves ◽  
Donna S. Turner ◽  
Kathy S. McKee ◽  
Buford L. Nichols ◽  
Julian P. Heath
Keyword(s):  
Sodium Ethoxide ◽  
Intracellular Localization ◽  
Protein A ◽  
Tween 20 ◽  
Intestinal Epithelial ◽  
Immunocytochemical Study ◽  
Fish Skin ◽  
Newborn Piglets ◽  
Rabbit Igg ◽  
Fish Skin Gelatin

In this study we used colloidal gold probes to detect the intracellular localization of colostral immunoglobulins in intestinal epithelial cells of newborn piglets.Tissues were obtained from non-suckled newborn and suckled piglets aged between 1 hour to 1 month. Samples were fixed in 2.5 % glutaraldehyde, osmicated and embedded into Spurr’s resin. Thin (80 nm) sections were etched with 5% sodium ethoxide for 5 min, washed and treated with 4 % sodium-m-periodate in distilled water for 30 min. The sections were then first incubated with blocking buffer (2 % BSA, 0.25 % fish skin gelatin, 0.5 % Tween 20 in 10 mM Trizma buffer, pH=7.4 containing 500 mM NaCl) for 30 min followed by the immunoreagents diluted in the same buffer, 1 hr each. For the detection of pig immunoglobulins a rabbit anti-pig IgG antiserum was used followed by goat anti-rabbit IgG-Au10 or protein A-Au15 probes.

STUDY OF PROTEIN COMPONENTS OF FISH SKIN MUCUS WITH THROMBOGENIC ACTIVITY

2020 ◽  
pp. 61-64
Author(s):  
L.L. Fomina ◽  
◽  
Yu.L. Oshurkova ◽  
O.A. Junina ◽  
T.S. Kulakova ◽  
...  
Keyword(s):  
Fish Skin ◽  
Skin Mucus ◽  
Protein Components

The Improvement of Food Quality and Safety of Fish Skin Crackers in Serangan Bali

2017 ◽  
Vol 3 (6) ◽  
pp. 119
Author(s):  
Ni Made Darmadi ◽  
Dewa Gede Semara Edi ◽  
I Made Kawan
Keyword(s):  
Moisture Content ◽  
Protein Content ◽  
Fat Content ◽  
Ash Content ◽  
Fish Skin ◽  
Economic Sectors ◽  
Food Quality And Safety ◽  
Processed Products ◽  
Micro Analysis ◽  
Flavor Enhancer

Indonesia’s government is attempting to improve the leading economic sectors such as the fishing industry, together with its processed products that could strengthen the national economy. Therefore, small industries should be able to improve the quality and security of their products for consumers’ assurance. Fish Skin Cracker is one of the products from domestic industry in Serangan, Bali, which made from Tuna and Shark. Those kinds of crackers are processed by adding spices, in which the spices would be different among processors and result in different quality. This research was aimed to investigate the best-quality fish skin cracker among the processors, based on the microbiological, chemical, and organoleptic analyzes. This research used Descriptive Method. Based on the results of analyzes, it was discovered that the best Tuna Skin Crackers were those from processor number 2 with values of quality analyzes; Moisture Content 6.1%, Ash Content 0.04%, Protein Content 59.33%, Fat Content 25.98%, TVB 14.21mg N %, TMA 7.45mg N%, Micro Analysis 4.9 x 104 Colony/gr, Organoleptic rates: texture 6, taste 7, aroma 6, and color 6. Processor number 2 used garlic, salt, and flavor enhancer as the seasoning. Meanwhile, the best Shark Skin Crackers were those from Processor number 5 with values of quality analyzes; Moisture Content 9.3%, Ash Content 0.04%, Protein Content 86.94%, Fat Content 3.7%, TVB 20.08mg N%, TMA 10.64mg N%, Micro Analysis 2.1 x 104 colony/gr, Organoleptic rates: texture 6, taste 6, smell 6, and color 6. Processor number 5 used garlic, coriander, turmeric, flavor enhancer, and lime juice as the seasoning.

scholarly journals Anti-Salmonella Activity and Peptidomic Profiling of Peptide Fractions Produced from Sturgeon Fish Skin Collagen (Huso huso) Using Commercial Enzymes

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2657
Author(s):  
Maryam Atef ◽  
Yasmina Ait Chait ◽  
Seyed Mahdi Ojagh ◽  
Ali Mohammad Latifi ◽  
Mina Esmaeili ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Degree Of Hydrolysis ◽  
Fish Skin ◽  
Small Peptides ◽  
Skin Collagen ◽  
Dependent Inhibition ◽  
Acetone Fractionation ◽  
Chain Lengths ◽  
Peptide Fractions ◽  
Fish Skin Collagen

This study investigated peptide fractions from fish skin collagen for antibacterial activity against Escherichia coli and Salmonella strains. The collagen was hydrolyzed with six commercial proteases, including trypsin, Alcalase, Neutrase, Flavourzyme, pepsin and papain. Hydrolyzed samples obtained with trypsin and Alcalase had the largest number of small peptides (molecular weight < 10 kDa), while the hydrolysate produced with papain showed the lowest degree of hydrolysis and highest number of large peptides. Four hydrolysates were found to inhibit the growth of the Gram-negative bacteria, with papain hydrolysate showing the best activity against E. coli, and Neutrase and papain hydrolysates showing the best activity against S. abony; hydrolysates produced with trypsin and pepsin did not show detectable antibacterial activity. After acetone fractionation of the latter hydrolysates, the peptide fractions demonstrated enhanced dose-dependent inhibition of the growth (colony-forming units) of four Salmonella strains, including S. abony (NCTC 6017), S. typhimurium (ATCC 13311), S. typhimurium (ATCC 14028) and S. chol (ATCC 10708). Shotgun peptidomics analysis of the acetone fractions of Neutrase and papain hydrolysates resulted in the identification of 71 and 103 peptides, respectively, with chain lengths of 6–22 and 6–24, respectively. This work provided an array of peptide sequences from fish skin collagen for pharmacophore identification, structure–activity relationship studies, and further investigation as food-based antibacterial agents against pathogenic microorganisms.

scholarly journals Fish and Chaps: Some Ethnoarchaeological Thoughts on Fish Skin Use in European Prehistory

2020 ◽  
Vol 6 (1) ◽  
pp. 329-347
Author(s):  
Roman Vávra
Keyword(s):  
Cultural Norms ◽  
Museum Collections ◽  
Fish Skin ◽  
Subsistence Strategies ◽  
Factors Affecting ◽  
Archaeological Data ◽  
The World

AbstractThe following study summarizes the available ethnographical, historical, and archaeological data about the use of fish skin as a material. Fish skin and the methods of its processing are described. The fish skin utilisation accounts from around the world are collected in an organized matter. Afterwards the various factors affecting the use of this material are discussed and some concluding remarks about the further research possibilities are proposed. The use of fish skin does not appear to be based purely on climate, subsistence strategies, or the availability of fish, but also on cultural norms and traditions. Careful hypotheses can be made by using relational analogies, but ultimately a re-examination of museum collections or new archaeological discoveries are fundamental in furthering this field of research.

scholarly journals Modulation of the Tissue Expression Pattern of Zebrafish CRP-Like Molecules Suggests a Relevant Antiviral Role in Fish Skin

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 78
Author(s):  
Melissa Bello-Perez ◽  
Mikolaj Adamek ◽  
Julio Coll ◽  
Antonio Figueras ◽  
Beatriz Novoa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacterial Infections ◽  
Quantitative Polymerase Chain Reaction ◽  
Tissue Expression ◽  
Interferon Response ◽  
Fish Skin ◽  
Tissue Expression Pattern ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Antiviral Role

Recent studies suggest that short pentraxins in fish might serve as biomarkers for not only bacterial infections, as in higher vertebrates including humans, but also for viral ones. These fish orthologs of mammalian short pentraxins are currently attracting interest because of their newly discovered antiviral activity. In the present work, the modulation of the gene expression of all zebrafish short pentraxins (CRP-like proteins, CRP1-7) was extensively analyzed by quantitative polymerase chain reaction. Initially, the tissue distribution of crp1-7 transcripts and how the transcripts varied in response to a bath infection with the spring viremia of carp virus, were determined. The expression of crp1-7 was widely distributed and generally increased after infection (mostly at 5 days post infection), except for crp1 (downregulated). Interestingly, several crp transcription levels significantly increased in skin. Further assays in mutant zebrafish of recombinant activation gene 1 (rag1) showed that all crps (except for crp2, downregulated) were already constitutively highly expressed in skin from rag1 knockouts and only increased moderately after viral infection. Similar results were obtained for most mx isoforms (a reporter gene of the interferon response), suggesting a general overcompensation of the innate immunity in the absence of the adaptive one.

scholarly journals Resistance reduction on trimaran ship model by biopolymer of eel slime

2015 ◽  
Vol 12 (2) ◽  
pp. 95-102
Author(s):  
Y. Yanuar ◽  
G. Gunawan ◽  
M. A. Talahatu ◽  
R. T. Indrawati ◽  
A. Jamaluddin
Keyword(s):  
Drag Reduction ◽  
Frictional Resistance ◽  
Skin Surface ◽  
Fish Skin ◽  
Total Drag ◽  
Towing Tank ◽  
Ship Model ◽  
Resistance Reduction ◽  
Towing Tank Test ◽  
Tank Test

Resistance reduction in ship becomes an important issue to be investigated. Energy consumption and its efficiency are related toward drag reduction. Drag reduction in fluid flow can be obtained by providing polymer additives, coating, surfactants, fiber and special roughness on the surface hull. Fish skin surface coated with biopolymers viscous fluid (slime) is one method in frictional resistance reduction. The aim of this is to understanding the effect of drag reduction using eel slime biopolymer in unsymmetrical trimaran ship model. The Investigation was conducted using towing tank test with variation of velocity. The dimension of trimaran model are L = 2 m, B = 0.20 m and T = 0.065 m. The ship model resistance was precisely measured by a load cell transducer. The comparison of resistance on trimaran ship model coated and uncoated by eel slime are shown on the graph as a function of the total drag coefficient and Froude number. It is discovered the trimaran ship model by eel slime has higher drag reduction compared to trimaran with no eel slime at similar displacement. The result shows the drag reduction about 11 % at Fr 0.35.

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