Malotilate Prevents Accumulation of Type III pN-Collagen, Type IV Collagen, and Laminin in Carbon Tetrachloride-induced Pulmonary Fibrosis in Rats

1989 ◽  
Vol 139 (5) ◽  
pp. 1105-1111 ◽  
Author(s):  
Paavo Pääkkö ◽  
Raija Sormunen ◽  
Leila Risteli ◽  
Juha Risteli ◽  
Leena Ala-Kokko ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Pulmonary Fibrosis ◽  
Type Iv Collagen ◽  
Collagen Type ◽  
Collagen Type Iv ◽  
Type Iii ◽  
Type Iv

scholarly journals Expression of collagen type IV in human kidney during prenatal development

2020 ◽  
pp. 111-111
Author(s):  
Vladimir Petrovic ◽  
Ivan Nikolic ◽  
Marko Jovic ◽  
Vladimir Zivkovic ◽  
Miodrag Jocic ◽  
...  
Keyword(s):  
Type Iv Collagen ◽  
Collagen Type ◽  
Kidney Tissue ◽  
Volume Density ◽  
Collagen Iv ◽  
Basement Membranes ◽  
Collagen Type Iv ◽  
Type Iv ◽  
Metanephric Kidney ◽  
Collecting System

Background / Aim. Type IV collagen belongs to the group of non-fibrillar collagens and is an important component of the basement membranes where it accounts for approximately 50% of its structural elements. The aim of the paper was to describe the expression and distribution of collagen type IV in embryonic and fetal metanephric kidney, and to determine the volume density of collagen type IV in kidney tissue in each trimester of development. Methods. The material consisted of 19 human embryos/fetuses, in the gestational age from 8th to 37th week. Kidney tissue specimens were routinely processed to paraffin molds and stained with hematoxylin and eosin and immunohistochemically using polyclonal anti-collagen IV antibody. Stained slides were examined using light microscope and images of the selected areas, under different lens magnification were captured with digital camera. Volume density of collagen type IV was determined by using ImageJ 1.48v and a plugin of the software which inserted a grid system with 336 points. For the data comparison One-Way Analysis of Variance was used. Results. Strong collagen IV immunopositivity was seen in all specimens, with a distribution in the basement membranes of urinary bud, parietal leaf of Bowman?s capsule, glomerular basement membrane, basement membrane of interstitial blood vessels, and basement membranes of nephron tubules and collecting ducts. No statistically significant difference in the volume density of type IV collagen was found between the different trimesters of development. Conclusion. The synthesis and secretion of collagen type IV simultaneously follows the development of nephron structures, collecting system and blood vessels. The volume density of collagen type IV remains constant throughout all the trimesters of metanephric kidney development, indicating that it plays a crucial role in normal development of nephron and collecting system structures, as well as in maintaining the normal kidney function.

Extracellular matrix components in the heart of the white bass, Morone chrysops (Rafinesque)

1994 ◽  
Vol 72 (3) ◽  
pp. 449-454
Author(s):  
Dominic S. Raso ◽  
Louis Terracio ◽  
Thomas K. Borg
Keyword(s):  
Type Iv Collagen ◽  
Collagen Type ◽  
Periodic Acid ◽  
Longitudinal Axis ◽  
Collagen Type Iv ◽  
Histological Techniques ◽  
White Bass ◽  
Morone Chrysops ◽  
Type Iv ◽  
Extracellular Matrix Components

The distribution of laminin, collagen type IV, collagen bundles, proteoglycans, elastin, and periodic acid–Schiff's moieties (glycoproteins) within the heart of the adult white bass, Morone chrysops (Rafinesque), was investigated by means of immunohistochemical and histological techniques. Laminin and collagen type IV were heavily expressed within the epimysium and the basal lamina of the lining epicardial epithelium and valvular endothelium, moderately expressed within the myocardium, and slightly expressed within the subendocardium. This co-localized distribution of laminin and collagen type IV corresponds to the biochemically unidentified basal and external lamina observed in the hearts of other fish by previous ultrastructural investigations and is similar to the distribution observed in the hearts of birds and mammals. Also demonstrated was an interesting division of connective tissue components along the longitudinal axis of the atrioventricular valve, which is most likely intimately involved with the effective functioning and durability of the valve.

Distribution of laminin, collagen type IV, collagen type I, and fibronectin in chicken cardiac jelly/basement membrane

1989 ◽  
Vol 224 (3) ◽  
pp. 417-425 ◽  
Author(s):  
Charles D. Little ◽  
Dominique M. Piquet ◽  
Lynn A. Davis ◽  
Luanne Walters ◽  
Christopher J. Drake
Keyword(s):  
Basement Membrane ◽  
Type Iv Collagen ◽  
Collagen Type ◽  
Collagen Type I ◽  
Collagen Type Iv ◽  
Type I ◽  
Type Iv

scholarly journals Intracellular signaling of transcription and secretion of type IV collagen after angiotensin II-induced cellular hypertrophy in cultured proximal tubular cells.

1991 ◽  
Vol 2 (3) ◽  
pp. 219-227 ◽  
Author(s):  
G Wolf ◽  
P D Killen ◽  
E G Neilson
Keyword(s):  
Angiotensin Ii ◽  
Type Iv Collagen ◽  
Collagen Type ◽  
Regulatory Elements ◽  
Collagen Type Iv ◽  
Intracellular Camp ◽  
Tubular Epithelium ◽  
Cellular Hypertrophy ◽  
Type Iv ◽  
Proximal Tubular

Physiologic concentrations of angiotensin II (AII) can induce cellular hypertrophy in murine proximal tubular epithelium (MCT cells). This response is characterized by an increase in cell size, new protein synthesis, and by the secretion of new basement membrane type IV collagen in the absence of cellular proliferation. The present study was undertaken to evaluate the second messengers of these AII-induced cellular events with special reference to the increase in type IV collagen secretion. In initial experiments we observed that pretreatment of MCT cells with agents that increase concentrations of intracellular cAMP, like forskolin, dibutyryl cAMP, and isobutyl-methyl-xanthine abolish AII-induced amino acid incorporation, but have no effect on control cells or on their proliferation. In addition, 10(-8) M AII significantly decreased the concentration of intracellular cAMP. Phorbolesters were without significant effect on the hypertrophy or proliferation of AII-stimulated MCT cells or their rested controls. The transfection of MCT cells with reporter genes containing regulatory elements for type IV collagen revealed that the stimulatory effects of AII on collagen type IV depend, at least to some extent, on an increase in gene transcription. Agents increasing intracellular cAMP concentrations inhibited the AII-induced increase in transcription and secretion of collagen type IV, but had no effect on MCT cells grown in media without AII. Our findings provide evidence that AII-induced changes in tubular epithelium leading to the secretion of type IV collagen are mediated by a decrease in intracellular cAMP.

Role of collagen type IV in the pathogenesis of increased prenasal thickness in Down syndrome fetuses: sonographic and immunohistological findings

2017 ◽  
Vol 45 (2) ◽  
Author(s):  
Ron Maymon ◽  
Sonia Mendlovic ◽  
Yaakov Melcer ◽  
Tal Sarig-Meth ◽  
Lilian Habler ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Blood Vessels ◽  
Type Iv Collagen ◽  
Collagen Type ◽  
Control Group ◽  
Collagen Type Iv ◽  
Type Iv ◽  
Products Of Conception ◽  
Tissue Specimens

AbstractObjective:The present study aims to compare the presence and localization of collagen type IV in the prenasal tissue of fetuses with and without Down syndrome (DS).Methods:Products of conception were obtained from mid-gestation uterine evacuations of 14 DS fetuses and 15 unaffected controls. Microdissection of the prenasal area and an analysis of the prenasal tissue specimens were performed by a single pathologist, blinded to the karyotype results. Immunohistological presence and localization of type IV collagen were analyzed in the basement membrane (BM), blood vessels, and stroma of the tissues.Results:There were no statistically significant differences in the presence and localization of antibodies for collagen IV in the blood vessels and stroma between the two groups. However, the presence and localization of type IV collagen in the BM of the prenasal skin were significantly higher in DS specimens compared to the control group (P=0.023). When combining both groups altogether, a significant correlation was found between the increased prenasal thickness (PT) and the high presence and location of collagen type IV, irrespective of the karyotype results (Spearman’s correlation; R=0.459; P=0.012).Conclusion:Using the immunohistochemistry technique, we were able to confirm the overexpression of collagen type IV in the BM of the prenasal area. This may explain the sonographic finding of increased PT seen mainly in DS fetuses.

scholarly journals Type IV Collagen, Type IV Collagenase Activity and Ability of Cell Proliferation in Human Thyroid Tumours

2002 ◽  
Vol 25 (4) ◽  
pp. 304-308 ◽  
Author(s):  
Takeshi Kusunoki ◽  
Shozo Nishida ◽  
Saori Kimoto-Kinoshita ◽  
Kiyotaka Murata ◽  
Takao Satou ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Type Iv Collagen ◽  
Collagen Type ◽  
Human Thyroid ◽  
Collagen Type Iv ◽  
Collagenase Activity ◽  
Type Iv Collagenase ◽  
Type Iv

scholarly journals Extracellular matrix proteins produced by stromal cells in idiopathic pulmonary fibrosis and lung adenocarcinoma

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250109
Author(s):  
Mervi Kreus ◽  
Siri Lehtonen ◽  
Sini Skarp ◽  
Riitta Kaarteenaho
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Lung Adenocarcinoma ◽  
Stromal Cells ◽  
Collagen Type ◽  
Normal Lung ◽  
Collagen Type Iv ◽  
Type Iv

Idiopathic pulmonary fibrosis (IPF) and lung cancer share common risk factors, epigenetic and genetic alterations, the activation of similar signaling pathways and poor survival. The aim of this study was to examine the gene expression profiles of stromal cells from patients with IPF and lung adenocarcinoma (ADC) as well as from normal lung. The gene expression levels of cultured stromal cells derived from non-smoking patients with ADC from the tumor (n = 4) and the corresponding normal lung (n = 4) as well as from patients with IPF (n = 4) were investigated with Affymetrix microarrays. The expression of collagen type IV alpha 1 chain, periostin as well as matrix metalloproteinase-1 and -3 in stromal cells and lung tissues were examined with quantitative real-time reverse transcriptase polymerase chain reaction and immunohistochemistry, respectively. Twenty genes were similarly up- or down-regulated in IPF and ADC compared to control, while most of the altered genes in IPF and ADC were differently expressed, including several extracellular matrix genes. Collagen type IV alpha 1 chain as well as matrix metalloproteinases-1 and -3 were differentially expressed in IPF compared to ADC. Periostin was up-regulated in both IPF and ADC in comparison to control. All studied factors were localized by immunohistochemistry in stromal cells within fibroblast foci in IPF and stroma of ADC. Despite the similarities found in gene expressions of IPF and ADC, several differences were also detected, suggesting that the molecular changes occurring in these two lung illnesses are somewhat different.

Use of a Biodegradable Neuroprosthesis Containing Laminin for Avulsive Peripheral Nerve Injury Results in Early Endoneurial and Perineurial Collagen Type III Formation

1990 ◽  
Vol 48 (3) ◽  
pp. 158-159
Author(s):  
B. Giammara ◽  
E. Anderson ◽  
P. Yates ◽  
J. Zuniga ◽  
J. Hanker
Keyword(s):  
Basement Membrane ◽  
Peripheral Nerve ◽  
Collagen Type ◽  
Collagen Type Iv ◽  
Type Iii Collagen ◽  
Distal Stump ◽  
Type Iii ◽  
Type Iv ◽  
Direct Anastomosis ◽  
Leg Movement

The direct anastomosis of nerve stumps is most desirable after peripheral nerve transection. There are many avulsive nerve injuries, however, where a nerve segment is destroyed or must be removed -- and the proximal and distal stumps are separated by a gap requiring bridging. Earlier studies showed that a biodegradable polyester mesh (polyglactin 910, Vicryl™) sleeve can act as a conduit and guide regenerating axons into the distal stump across an 11 mm gap created in rat sciatic nerve. This is facilitated by filling the sleeve with Matrigel™, an extracellular basement membrane gel containing 80% laminin, smaller amounts of collagen type IV, heparan sulfate proteoglycan and entaction. The avulsed sciatic was reconnected in animals examined after 30 and 45 days and sensation and leg movement were much improved.The PATS variation of the PAS reaction can be used to demonstrate all glycomacromolecules containing alpha-diglycol groups. Although it also stains basement membrane or type IV collagen brown, it stains type III collagen or reticulin intensely black.

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