Achnanthidium sinense sp. nov. (Bacillariophyta) from the Wuling Mountains Area, China

Phytotaxa ◽  
2016 ◽  
Vol 284 (3) ◽  
pp. 194 ◽  
Author(s):  
BING LIU ◽  
SAÚL BLANCO ◽  
HUA LONG ◽  
JINGJING XU ◽  
XIAOYAN JIANG

Achnanthidium sinense sp. nov., a new diatom species from the Wuling Mountains Area, central China, was studied by light and scanning electron microscopy. The valvar dimensions of this species are relatively large with respect to average Achnathidium taxa. The valves are narrowly lanceolate with round, acute apices. Only a few striae in the middle part of both valves can be resolved under light microscopy. The axial area of raphe valve is linear-lanceolate and the distal raphe fissures hook towards the same side of the valve. Striae are slightly radiate throughout both valves except at the apices of the raphe valve, where they become parallel or slightly convergent. The number of areolae in each stria on the rapheless valve is usually greater than that on the raphe valve (1–7 vs. 1–6). Around the central areas of both valves, sometimes short marginal striae composed of 1–3 areolae are present. Valve mantle possesses a row of slit-like areolae and 2–3 notches. A. sinense is compared with the similar species A. exile and other large species. A. sinense is epilithic and lives in freshwater habitats.

2019 ◽  
Vol 152 (2) ◽  
pp. 340-350
Author(s):  
Carlos E. Wetzel ◽  
Ingrid Jüttner ◽  
Smriti Gurung ◽  
Luc Ector

Background and aims – Two unknown benthic diatom species belonging to the genus Achnanthidium Kütz., and found in French and Nepalese freshwater habitats, were investigated. Both species are here described as new and compared with the original material of Achnanthes minutissima var. macrocephala Hust. [≡ Achnanthidium macrocephalum (Hust.) Round & Bukht.] from Indonesia. Methods – The morphology of three small and capitate Achnanthidium species was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Key results – Achnanthidium coxianum sp. nov. (from the Himalaya) belongs to the group of species with hooked terminal raphe endings, while Achnanthidium peetersianum sp. nov. (from France) has variable terminal raphe endings which are usually slightly bent. Achnanthidium macrocephalum is a much rarer species and illustrations concerning its identity in the literature do not conform to the type studied here. Conclusions – The three species are similar in LM but clearly distinct in SEM. The shape of the areolae and terminal raphe endings separate the species. Achnanthidium macrocephalum has often been misidentified in studies from many areas of the world. It is similar to the new species in valve outline and in its small dimensions.


Phytotaxa ◽  
2019 ◽  
Vol 405 (3) ◽  
pp. 147
Author(s):  
P. YU ◽  
Q-M. YOU ◽  
W-T. PANG ◽  
Y. CAO ◽  
Q-X. WANG

Five new species are described in this study: Achnanthidium jiuzhaienis sp. nov., Achnanthidium epilithica sp. nov., Achnanthidium limosua sp. nov., Achnanthidium subtilissimum sp. nov., and Kolbesia sichuanenis sp. nov. Classification was based on light microscopy (LM) and scanning electron microscopy (SEM). A. jiuzhaienis sp. nov. belongs to the “A. pyrenaicum complex” of the genus Achnanthidium, this classification was based on certain characteristics, such as the possession of transpically-elongated areolae and deflected external distal raphe fissures. A. epilithica sp. nov., A. limosua sp. nov., and A. subtilissimum sp. nov. belong to the “A. minutissimum complex” of the genus Achnanthidium, this classification was based on certain characteristics, such as the possession of straight external distal raphe fissures and round or elliptical areolae. Kolbesia sichuanenis sp. nov. was found to belongs to the Kolbesia genus, based on its elongated areolae and striae comprised of one macroareola. All five species were sufficiently different from other similar species to be considered unique separate species based on their valve outline, shape of the axial and center areas, and density of striae of each taxon. These new species derived from the epilithic and epiphytic collections of four alkaline lakes in Jiuzhai Valley, Sichuan Province, Southwest China.


Phytotaxa ◽  
2020 ◽  
Vol 450 (2) ◽  
pp. 209-218
Author(s):  
WEIWEI WU ◽  
HUINA LIN ◽  
VISHAL PATIL ◽  
JOHN PATRICK KOCIOLEK ◽  
LIN SUN ◽  
...  

Halamphora yundangensis sp. nov. is described as a new species epiphytic on the green seaweed Ulva lactuca, from Yundang Lake, Fujian Province, China. Morphological details of the new species with respect to shape, size and valve ultrastructure are presented, with observations based on light microscopy (LM) and scanning electron microscopy (SEM). The main features of Halamphora yundangensis are dorsiventral, semi-lanceolate valves with convex dorsal margin and straight ventral margin, as well as valve ends distinctly protracted and capitate. Externally, a prominent marginal ridge forming the image of a broad hyaline area is present. Internally, dorsal striae are regularly biseriate, and a row of areolae lies along raphe. The new species is compared with similar species in both Amphora and Halamphora.


Mycotaxon ◽  
2020 ◽  
Vol 134 (4) ◽  
pp. 643-648
Author(s):  
Chaofeng Yuan ◽  
Shu Li ◽  
Wan Wang ◽  
Shuwei Wei ◽  
Qi Wang ◽  
...  

Didymium inconspicuum, D. karstensii, and D. rugulosporum are reported as new records for China. Morphological characteristics were observed and described by light microscopy and scanning electron microscopy and are discussed and compared with similar species.


Phytotaxa ◽  
2012 ◽  
Vol 66 (1) ◽  
pp. 43
Author(s):  
BART VAN DE VIJVER ◽  
GEURT L. VERWEIJ ◽  
JAKO VAN DER WAL ◽  
ADRIENNE MERTENS

A new cymbelloid diatom species, Encyonopsis neerlandica sp. nov., is described from several moorland pools from The Netherlands. The new species belongs to the complex of species around E. microcephala. Based on light and detailed scanning electron microscopy, the morphology of Encyonopsis neerlandica is discussed and compared to similar species from this complex. Encyonopsis neerlandica is characterized by its rather large valve dimensions, the narrowly to clearly lanceolate valve outline with convex to weakly convex margins and rostrate to subcapitate apices. The distal raphe fissures are ventrally deflected. There is a clear difference between the rounded areolae near the valve margin and the transapically elongated areolae near the axial area.Notes on the ecology of the species are included.


Phytotaxa ◽  
2017 ◽  
Vol 298 (1) ◽  
pp. 43 ◽  
Author(s):  
BING LIU ◽  
DAVID M. WILLIAMS ◽  
YONGDA OU

Adlafia sinensis sp. nov., a new diatom species from the Wuling Mountains Area, China, has been studied using light and scanning electron microscopy. The valves are lanceolate with rostrate to subcapitate apices; the areolae are occluded by hymens outside and the both terminal raphe fissures hook towards the secondary side; the mantle is deep with c. 5–7 isolated striae not continuous from the valve face. Only one girdle band (valvocopula) occurs between the epivalve and hypovalve except at the apices of each frustule. The nature of the valvocopula is open because the two parts are not fused together. A. sinensis is compared with the all species in the genus Adlafia. A. sinensis is epilithic and live in freshwater habitats.


Phytotaxa ◽  
2018 ◽  
Vol 357 (1) ◽  
pp. 41 ◽  
Author(s):  
JULIEN MARQUIE ◽  
RENE LE COHU ◽  
MICHEL COSTE

During a recent survey of epilithic diatoms in running waters of New Caledonia, two unknown Adlafia Moser, Lange-Bertalot & Metzeltin species were found. Both species are described as new based on light and scanning electron microscopy (LM and SEM). Adlafia moseri sp. nov., is characterised by its margins morphology, its large size and its low stria density, clearly visible in LM. Adlafia tjibaoui sp. nov. shows a very long internal intermissio with curved proximal raphe endings and a very high stria density. Since the generitype Adlafia muscora (Kociolek & de Reviers) Moser, Lange-Bertalot & Metzeltin was poorly described, its diagnosis is emended based on more detailed scanning electron microscopy observations. Kobayasiella saxicola (Manguin) Lange-Bertalot, only described in light microscopy, occurs frequently in some running waters in New Caledonia. An emended description of this taxon is likewise proposed based on additional SEM data.


Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).


Author(s):  
D. Johnson ◽  
P. Moriearty

Since several species of Schistosoma, or blood fluke, parasitize man, these trematodes have been subjected to extensive study. Light microscopy and conventional electron microscopy have yielded much information about the morphology of the various stages; however, scanning electron microscopy has been little utilized for this purpose. As the figures demonstrate, scanning microscopy is particularly helpful in studying at high resolution characteristics of surface structure, which are important in determining host-parasite relationships.


Author(s):  
D.R. Hill ◽  
J.R. McCurry ◽  
L.P. Elliott ◽  
G. Howard

Germination of Euonymous americanus in the laboratory has previously been unsuccessful. Ability to germinate Euonymous americanus. commonly known as the american strawberry bush, is important in that it represents a valuable food source for the white-tailed deer. Utilizing the knowledge that its seeds spend a period of time in the rumin fluid of deer during their dormant stage, we were successful in initiating germination. After a three month drying period, the seeds were placed in 25 ml of buffered rumin fluid, pH 8 at 40°C for 48 hrs anaerobically. They were then allowed to dry at room temperature for 24 hrs, placed on moistened filter paper and enclosed within an environmental chamber. Approximately four weeks later germination was detected and verified by scanning electron microscopy; light microscopy provided inadequate resolution. An important point to note in this procedure is that scarification, which was thought to be vital for germination, proved to be unnecessary for successful germination to occur. It is believed that germination was propagated by the secretion of enzymes or prescence of acids produced by microorganisms found in the rumin fluid since sterilized rumin failed to bring about germination.


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