New records of Didymium inconspicuum , D. karstensii , and D. rugulosporum from China

Mycotaxon ◽  
2020 ◽  
Vol 134 (4) ◽  
pp. 643-648
Author(s):  
Chaofeng Yuan ◽  
Shu Li ◽  
Wan Wang ◽  
Shuwei Wei ◽  
Qi Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
New Records ◽  
Morphological Characteristics ◽  
Similar Species ◽  
Scanning Electron

Didymium inconspicuum, D. karstensii, and D. rugulosporum are reported as new records for China. Morphological characteristics were observed and described by light microscopy and scanning electron microscopy and are discussed and compared with similar species.

Five new Achnanthidiaceae species (Bacillariophyta) from Jiuzhai Valley, Sichuan Province, Southwestern China

Phytotaxa ◽  
2019 ◽  
Vol 405 (3) ◽  
pp. 147
Author(s):  
P. YU ◽  
Q-M. YOU ◽  
W-T. PANG ◽  
Y. CAO ◽  
Q-X. WANG
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Light Microscopy ◽  
Southwest China ◽  
Sichuan Province ◽  
Similar Species ◽  
Separate Species ◽  
Outline Shape ◽  
Scanning Electron

Five new species are described in this study: Achnanthidium jiuzhaienis sp. nov., Achnanthidium epilithica sp. nov., Achnanthidium limosua sp. nov., Achnanthidium subtilissimum sp. nov., and Kolbesia sichuanenis sp. nov. Classification was based on light microscopy (LM) and scanning electron microscopy (SEM). A. jiuzhaienis sp. nov. belongs to the “A. pyrenaicum complex” of the genus Achnanthidium, this classification was based on certain characteristics, such as the possession of transpically-elongated areolae and deflected external distal raphe fissures. A. epilithica sp. nov., A. limosua sp. nov., and A. subtilissimum sp. nov. belong to the “A. minutissimum complex” of the genus Achnanthidium, this classification was based on certain characteristics, such as the possession of straight external distal raphe fissures and round or elliptical areolae. Kolbesia sichuanenis sp. nov. was found to belongs to the Kolbesia genus, based on its elongated areolae and striae comprised of one macroareola. All five species were sufficiently different from other similar species to be considered unique separate species based on their valve outline, shape of the axial and center areas, and density of striae of each taxon. These new species derived from the epilithic and epiphytic collections of four alkaline lakes in Jiuzhai Valley, Sichuan Province, Southwest China.

Comparison of Larval Stored Product Beetle Mandibles by Light Microscope and Scanning Electron Microscope

1981 ◽  
Vol 64 (1) ◽  
pp. 199-224
Author(s):  
John E Kvenberg
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Morphological Characteristics ◽  
Scanning Electron ◽  
Conventional Light Microscopy

Abstract Larval stored product beetle mandibles were studied by comparing images made by scanning electron microscopy with those made by conventional light microscopy. Discussion of morphological characteristics is based on illustrations of 25 species

Achnanthidium sinense sp. nov. (Bacillariophyta) from the Wuling Mountains Area, China

Phytotaxa ◽  
2016 ◽  
Vol 284 (3) ◽  
pp. 194 ◽  
Author(s):  
BING LIU ◽  
SAÚL BLANCO ◽  
HUA LONG ◽  
JINGJING XU ◽  
XIAOYAN JIANG
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Middle Part ◽  
Central China ◽  
Similar Species ◽  
Large Species ◽  
Diatom Species ◽  
Freshwater Habitats ◽  
Scanning Electron

Achnanthidium sinense sp. nov., a new diatom species from the Wuling Mountains Area, central China, was studied by light and scanning electron microscopy. The valvar dimensions of this species are relatively large with respect to average Achnathidium taxa. The valves are narrowly lanceolate with round, acute apices. Only a few striae in the middle part of both valves can be resolved under light microscopy. The axial area of raphe valve is linear-lanceolate and the distal raphe fissures hook towards the same side of the valve. Striae are slightly radiate throughout both valves except at the apices of the raphe valve, where they become parallel or slightly convergent. The number of areolae in each stria on the rapheless valve is usually greater than that on the raphe valve (1–7 vs. 1–6). Around the central areas of both valves, sometimes short marginal striae composed of 1–3 areolae are present. Valve mantle possesses a row of slit-like areolae and 2–3 notches. A. sinense is compared with the similar species A. exile and other large species. A. sinense is epilithic and lives in freshwater habitats.

scholarly journals Globocephalus urosubulatus (Alessandrini, 1909) (Nematoda: Ancylostomatidae) in Brazil: a morphological revisitation

2021 ◽  
Vol 30 (3) ◽  
Author(s):  
Raul Henrique da Silva Pinheiro ◽  
Sérgio Melo ◽  
Raimundo Nonato Moraes Benigno ◽  
Elane Guerreiro Giese
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Sus Scrofa ◽  
Production Systems ◽  
Morphological Characteristics ◽  
Morphological Data ◽  
Domestic Pigs ◽  
Taxonomic Studies ◽  
Scanning Electron

Abstract Helminthiasis occurs frequently in wild or domestic pigs in the most varied environments or production systems; however, the literature includes few taxonomic studies for the purpose of expanding this knowledge about the parasitic diversity in these animals. Thus, in order to expand this knowledge regarding parasitic diversity in these animals, the present study reports the occurrence and redescribes Globocephalus urosubulatus infecting domestic pigs in the state of Pará, Brazil, using microscopy. Four hundred and ten specimens of Sus scrofa intestinal nematodes were collected extensively in the municipality of Moju, northeastern mesoregion of Pará and observed under light and scanning electron microscopy. Based on morphological characteristics, the nematodes were identified as G. urosubulatus, and new morphological data were added by light microscopy and scanning electron microscopy for the diagnosis of this parasite.

scholarly journals Morphology and infection process of Olivea neotectonae in teak leaves

2019 ◽  
Vol 86 ◽  
Author(s):  
Pedro Raymundo Argüelles Osorio ◽  
Evelynne Urzêdo Leão ◽  
Talita Pereira de Souza Ferreira ◽  
Marcos Vinicius Giongo Alves ◽  
Renato de Almeida Sarmento ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Morphological Characteristics ◽  
Infection Process ◽  
Basic Information ◽  
Teak Plantations ◽  
Microscopy Techniques ◽  
Important Disease ◽  
Scanning Electron

ABSTRACT: Rust, caused by the biotrophic fungus Olivea neotectonae, stands out as an important disease in teak plantations in Brazil. Considering the current importance of this rust and the need to obtain additional basic information about its causal agent to better disease management, the present study aimed to analyze the morphology of O. neotectonae using light microscopy and demonstrate the infection process in teak leaves using scanning electron microscopy techniques (SEM). The morphological characteristics observed using light microscopy were similar to those described in the literature for this species. SEM revealed that the infection structures of the pathogen penetrated the teak leaf through the stomata complex. This study provides the first microscopic evidence of the infection process of Olivea neotectonae in leaves of teak plants.

Early-stage microvascular alterations of a new model of controlled cortical traumatic brain injury: 3D morphological analysis using scanning electron microscopy and corrosion casting

2013 ◽  
Vol 118 (4) ◽  
pp. 763-774 ◽  
Author(s):  
Simone Sangiorgi ◽  
Alessandro De Benedictis ◽  
Marina Protasoni ◽  
Alessandro Manelli ◽  
Marcella Reguzzoni ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Traumatic Brain Injury ◽  
Scanning Electron Microscopy ◽  
Brain Injury ◽  
Light Microscopy ◽  
Morphological Characteristics ◽  
Corrosion Casting ◽  
Brain Swelling ◽  
Microvascular Alterations ◽  
Scanning Electron

Object This study was performed to study the microvascular changes that occur during the first 12 hours after traumatic brain injury (TBI) using the corrosion casting technique. Methods The authors performed a qualitative and quantitative morphological study of the changes in cerebral vessels at acute (3 hours) and subacute (12 hours) stages after experimental TBI. They used a model of controlled cortical impact (CCI) injury induced by a recently developed electromagnetic device (impactor), focusing their observations mainly on the microvascular alterations responsible for the formation and maintenance of tissue edema and consequent brain swelling during the first hours after TBI. They used corrosion casting, scanning electron microscopy (SEM), light microscopy, and transmission electron microscopy (TEM) to obtain a morphological qualitative map with both 2D and 3D details. Results Scanning electron microscopy analysis of vascular casts documented in 3 dimensions the typical injuries occurring after a TBI: subdural, subarachnoid, and intraparenchymal hemorrhages, along with alterations of the morphological characteristics and architecture of both medium-sized and capillary vessels, including ectasia of pial vessels, sphincter constrictions at the origin of the perforating vessels, focal swelling of perforating vessels, widening of intercellular junctions, and some indirect evidence of structural impairment of endothelial cells. All of these vascular alterations were confirmed in 2D analyses using light microscopy and TEM. Conclusions The corrosion casting–SEM technique applied to a CCI experimental model proved to be a reliable method for studying the pathophysiology of the vascular alterations occurring at acute and subacute stages after CCI injury. It was also possible to obtain topographical localization of the vascular and cellular events that usually lead to hyperemia, edema, and brain swelling. Moreover, by applying informatic software to anatomical images it was possible to perform quantification and statistical analysis of the observed events.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Examination of Schistosome Cercariae and Schistosomules by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 50-51
Author(s):  
D. Johnson ◽  
P. Moriearty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Surface Structure ◽  
Extensive Study ◽  
Scanning Microscopy ◽  
Host Parasite ◽  
Conventional Electron Microscopy ◽  
Scanning Electron

Since several species of Schistosoma, or blood fluke, parasitize man, these trematodes have been subjected to extensive study. Light microscopy and conventional electron microscopy have yielded much information about the morphology of the various stages; however, scanning electron microscopy has been little utilized for this purpose. As the figures demonstrate, scanning microscopy is particularly helpful in studying at high resolution characteristics of surface structure, which are important in determining host-parasite relationships.

SEM Observations on the Germination of Euonymous Americanus

1985 ◽  
Vol 43 ◽  
pp. 508-509
Author(s):  
D.R. Hill ◽  
J.R. McCurry ◽  
L.P. Elliott ◽  
G. Howard
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Filter Paper ◽  
Room Temperature ◽  
Food Source ◽  
Environmental Chamber ◽  
Dormant Stage ◽  
Scanning Electron

Germination of Euonymous americanus in the laboratory has previously been unsuccessful. Ability to germinate Euonymous americanus. commonly known as the american strawberry bush, is important in that it represents a valuable food source for the white-tailed deer. Utilizing the knowledge that its seeds spend a period of time in the rumin fluid of deer during their dormant stage, we were successful in initiating germination. After a three month drying period, the seeds were placed in 25 ml of buffered rumin fluid, pH 8 at 40°C for 48 hrs anaerobically. They were then allowed to dry at room temperature for 24 hrs, placed on moistened filter paper and enclosed within an environmental chamber. Approximately four weeks later germination was detected and verified by scanning electron microscopy; light microscopy provided inadequate resolution. An important point to note in this procedure is that scarification, which was thought to be vital for germination, proved to be unnecessary for successful germination to occur. It is believed that germination was propagated by the secretion of enzymes or prescence of acids produced by microorganisms found in the rumin fluid since sterilized rumin failed to bring about germination.

New diagnostic characters of Kolhymorbis angarensis (Dybowski & Grochmalicki, 1925) (Gastropoda: Pulmonata: Planorbidae)

2009 ◽  
Vol 18 (2) ◽  
pp. 191-195
Author(s):  
E.V. Soldatenko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Diagnostic Characters ◽  
Copulatory Apparatus ◽  
Scanning Electron

The radula morphology and the anatomy of the copulatory apparatus in Kolhymorbis angarensis were examined using light microscopy, scanning electron microscopy (SEM) and histological methods. Kolhymorbis angarensis was shown to have the stylet and the penial sac with a glandular appendage (flagellum), the characteristics, previously unknown for any species of this genus. The significance of these findings for the taxonomy of the genus is discussed.

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