Diversispora valentina (Diversisporaceae), a new species of arbuscular mycorrhizal fungi from the Mediterranean sand dunes of Spain

A new arbuscular mycorrhizal fungal species, Diversispora valentina, is described and illustrated. In the field, this species is associated with marine dunes located along the Mediterranean coast in eastern Spain. Spores of D. valentina occurred in sporocarps, in clusters, and singly in the soil or inside the roots of Ammophila arenaria (Poaceae), Elymus farctus (Poaceae), Otanthus maritimus (Asteraceae), and Echinophora spinosa (Apiaceae) in the six locations studied. A single-species culture of D. valentina was obtained using Trifolium repens as a host plant. The small subunit internal transcribed spacer and large subunit (SSU-ITS1-5.8S-ITS2-LSU) nrDNA sequences place the new species in the genus Diversispora and suggest that it differs from any previously described species. The novelty of this species is supported by morphological, molecular, and phylogenetic analyses.

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Glomus achrumandG. bistratum,two new species of arbuscular mycorrhizal fungi (Glomeromycota) found in maritime sand dunes

Botany ◽

10.1139/b08-138 ◽

2009 ◽

Vol 87 (3) ◽

pp. 260-271 ◽

Cited By ~ 9

Author(s):

Janusz Błaszkowski ◽

Przemysław Ryszka ◽

Fritz Oehl ◽

Sally Koegel ◽

Andres Wiemken ◽

...

Keyword(s):

Arbuscular Mycorrhiza ◽

Mycorrhizal Fungi ◽

Phylogenetic Analyses ◽

Plantago Lanceolata ◽

Sand Dunes ◽

Arbuscular Mycorrhizal ◽

Its Region ◽

Ammophila Arenaria ◽

Vesicular Arbuscular Mycorrhiza ◽

Vesicular Arbuscular

Two new arbuscular mycorrhizal fungal species, Glomus achrum sp. nov. and Glomus bistratum sp. nov. (Glomeromycota), are described and illustrated. Both species produce small, hyaline spores in aggregates formed in the soil and inside roots. Glomus achrum was associated with roots of Ammophila arenaria (L.) Link colonizing maritime dunes of the Vistula Bar in northern Poland, and G. bistratum occurred among vesicular-arbuscular mycorrhiza of Xanthium cf. spinosum growing in dunes of the Mediterranean Sea adjacent to Veriko, Greece. Spores of G. achrum are globose to subglobose, (25–)43(–55) µm in diameter, rarely egg-shaped, oblong to irregular, 15–45 µm × 55–65 µm. Their wall consists of three hyaline layers: a mucilaginous, short-lived outermost layer; a laminate middle layer composed of loose sublayers; and a flexible innermost layer. The outermost and the innermost layers stain deeply red in Melzer’s reagent. Spores of G. bistratum are globose to subglobose, (20–)29(–50) µm in diameter, and have a wall composed of two permanent, hyaline layers. The outer layer is unit, smooth, and the inner one laminate. Only the inner layer stains yellow in Melzer’s reagent. Both species formed vesicular-arbuscular mycorrhiza in single-species cultures with Plantago lanceolata L. as the host plant. Phylogenetic analyses of partial 18S rDNA subunit and internal transcribed spacer (ITS) region sequences placed G. achrum and G. bistratum into Glomus group A, but did not reveal any closely related described species. Environmental sequences from the public databases suggested that G. achrum occurred in at least two other plant species from geographically distant regions. No such evidence could be obtained for G. bistratum, which is currently known only from the type location.

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Septoglomus deserticola emended and new combinations in the emended definition of the family Diversisporaceae

Acta Mycologica ◽

10.5586/am.2013.011 ◽

2013 ◽

Vol 48 (1) ◽

pp. 89-103 ◽

Cited By ~ 13

Author(s):

Janusz Błaszkowski ◽

Gerard Chwat

Keyword(s):

Phylogenetic Analyses ◽

Large Subunit ◽

Sand Dunes ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Original Description ◽

Small Subunit ◽

The Family ◽

Definition Of ◽

Lsu Nrdna

An updated morphology of spores of Septoglomus deserticola, an arbuscular mycorrhizal fungus of the phylum Glomeromycota, is presented based on the original description of the species, only one other its definition recently published and spores produced in pot cultures inoculated with the rhizosphere soil and root fragments of an unrecognized grass colonizing maritime sand dunes of the Hicacos Peninsula, Cuba. Phylogenetic analyses of sequences of the large subunit (LSU) nrDNA region of the Cuban fungus confirmed its affinity with S. deserticola deposited in the International Bank for the Glomeromycota (BEG) and indicated that its closest relatives are S. fuscum and S. xanthium. Phylogenetic analyses of sequences of the small subunit (SSU) nrDNA confirmed the Cuban fungus x S. fuscum x S. xanthium relationship revealed in analyses of the LSU sequences and thereby suggested the Cuban Septoglomus is S. deserticola. However, it was impossible to prove directly the identity of the Cuban fungus and S. deserticola from BEG based on SSU sequences due to the lack of S. deserticola SSU sequences in public databases. In addition, phylogenetic analyses of LSU and SSU sequences confirmed the uniqueness of the recently erected genus Corymbiglomus with the type species C. corymbiforme (formerly Glomus corymbiforme) in the family Diversisporaceae and proved that its LSU sequences group in a clade with LSU sequences of G. globiferum and G. tortuosum. Consequently, the two latter species were transferred to Corymbiglomus and named C. globiferum comb. nov. and C. tortuosum comb. nov., and the definitions of the family Diversisporaceae and the genus Diversispora were emended.

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Dominikia emiratia and Rhizoglomus dunense, two new species in the Glomeromycota

Botany ◽

10.1139/cjb-2016-0294 ◽

2017 ◽

Vol 95 (7) ◽

pp. 629-639 ◽

Cited By ~ 4

Author(s):

Mohamed N. Al-Yahya’ei ◽

Sangeeta Kutty Mullath ◽

Laila A. AlDhaheri ◽

Anna Kozłowska ◽

Janusz Błaszkowski

Keyword(s):

New Species ◽

United Arab Emirates ◽

Mycorrhizal Fungi ◽

Phylogenetic Analyses ◽

Sand Dunes ◽

Spore Wall ◽

Wall Layer ◽

Ammophila Arenaria ◽

Rpb1 Gene ◽

Two New Species

The morphological, histochemical, and molecular properties of two new species of arbuscular mycorrhizal fungi (AMF; Glomeromycota) have been characterized. The first species is distinguished by spores that are orange to brownish orange, small, and formed only in clusters and mainly by having two laminate layers in a three-layered spore wall, with layer three staining dark in Melzer’s reagent. Despite the morphological similarity to some Septoglomus spp., phylogenetic analyses of sequences of the SSU–ITS–LSU nrDNA region and the RPB1 gene accommodated the fungus in the genus Dominikia, hence it was named Dominika emiratia. Intact spores of the second species, named Rhizoglomus dunense, closely resemble colourless isolates of R. clarum, but their spore wall layer three never becomes coloured with age, as does that in most R. clarum spores, and most importantly, the two fungi are separated by a large molecular distance. Dominikia emiratia was originally extracted from the rhizosphere of three plant species cultivated in two fields in a sandy desert in the Emirate of Abu Dhabi of the United Arab Emirates. Rhizoglomus dunense was found in a trap culture inoculated with the rhizosphere soil and root fragments of Ammophila arenaria, which had colonized sand dunes of the Mediterranean Sea, located near Thessalonica, Greece.

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Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

Parasites & Vectors ◽

10.1186/s13071-020-04430-6 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Yi-Fan Cao ◽

Hui-Xia Chen ◽

Yang Li ◽

Dang-Wei Zhou ◽

Shi-Long Chen ◽

...

Keyword(s):

New Species ◽

Ribosomal Dna ◽

Dna Sequences ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Small Subunit ◽

Sister Relationship ◽

Tibetan Antelope ◽

A New Species

Abstract Background The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites of the Tibetan antelope remains limited. Methods Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences, i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1), were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes. Results Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum n. sp. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions A new species of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum n. sp. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

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Dominikia duoreactiva sp. nov. and Dominikia difficilevidera sp. nov., two new species in the Glomeromycota

Botany ◽

10.1139/cjb-2015-0016 ◽

2015 ◽

Vol 93 (7) ◽

pp. 389-396 ◽

Cited By ~ 6

Author(s):

Janusz Błaszkowski ◽

Gerard Chwat ◽

Sarah Symanczik ◽

Anna Góralska

Keyword(s):

New Species ◽

Mycorrhizal Fungi ◽

National Park ◽

Phylogenetic Analyses ◽

Arbuscular Mycorrhizal ◽

Morphological Characters ◽

Spore Wall ◽

Worldwide Distribution ◽

Two New Species ◽

Dune Plants

Two new species of arbuscular mycorrhizal fungi (AMF) of the recently erected genus Dominikia (Glomeromycota) are described based on their morphology and phylogenetic analyses of SSU–ITS–LSU sequences. The distinctive morphological characters of the first species, Dominikia duoreactiva sp. nov., is the formation of loose clusters with yellow-coloured, 30–70 μm diameter spores having a three-layered spore wall, of which layers 1 and 3 stain in Melzer’s reagent. The second species, Dominikia difficilevidera sp. nov., is distinguished by its hyaline, 31–45 μm diameter spores, which arise mainly singly and have a three-layered spore wall, of which layer 1 is thicker than the structural laminate layer 2, and layer 3 is flexible to semi-flexible. Both species were originally associated with maritime dune plants; D. duoreactiva comes from the Giftun Island, Egypt, Africa, and D. difficilevidera from the Słowiński National Park, Poland. Based on available data, we suggest D. duoreactiva occurs rarely in the world, and D. difficilevidera has a worldwide distribution, but it either occurs infrequently or has been overlooked or lost during spore extraction from soils of many sites because of its extremely small and hyaline spores. A method allowing the extraction of even the smallest spores of AMF, but observable under a dissecting microscope, is described.

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Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota)

MycoKeys ◽

10.3897/mycokeys.82.63241 ◽

2021 ◽

Vol 82 ◽

pp. 33-56

Author(s):

Long-Fei Fan ◽

Renato Lúcio Mendes Alvarenga ◽

Tatiana Baptista Gibertoni ◽

Fang Wu ◽

Yu-Cheng Dai

Keyword(s):

New Species ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Molecular Data ◽

Small Subunit ◽

Molecular Evidence ◽

Rrna Gene

Samples of species close to Tremella fibulifera from China and Brazil are studied, and T. fibulifera is confirmed as a species complex including nine species. Five known species (T. cheejenii, T. fibulifera s.s., T. “neofibulifera”, T. lloydiae-candidae and T. olens) and four new species (T. australe, T. guangxiensis, T. latispora and T. subfibulifera) in the complex are recognized based on morphological characteristics, molecular evidence, and geographic distribution. Sequences of eight species of the complex were included in the phylogenetic analyses because T. olens lacks molecular data. The phylogenetic analyses were performed by a combined sequence dataset of the internal transcribed spacer (ITS) and the partial nuclear large subunit rDNA (nLSU), and a combined sequence dataset of the ITS, partial nLSU, the small subunit mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α (TEF1), the largest and second largest subunits of RNA polymerase II (RPB1 and RPB2). The eight species formed eight independent lineages with robust support in phylogenies based on both datasets. Illustrated description of the six species including Tremella fibulifera s.s., T. “neofibulifera” and four new species, and discussions with their related species, are provided. A table of the comparison of the important characteristics of nine species in the T. fibulifera complex and a key to the whitish species in Tremella s.s. are provided.

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Multi-Gene Phylogeny and Taxonomy of Hydnellum (Bankeraceae, Basidiomycota) from China

Journal of Fungi ◽

10.3390/jof7100818 ◽

2021 ◽

Vol 7 (10) ◽

pp. 818

Author(s):

Yan-Hong Mu ◽

Jia-Rui Yu ◽

Ting Cao ◽

Xiang-Hua Wang ◽

Hai-Sheng Yuan

Keyword(s):

New Species ◽

Rna Polymerase Ii ◽

Ribosomal Rna ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Morphological Characteristics ◽

Small Subunit ◽

Phylogenetic Position ◽

Ribosomal Rna Gene ◽

Morphological Studies

The genus Hydnellum is an important group of stipitate hydnaceous fungi which can form ectomycorrhiza with many species of woody plants. In recent decades, the frequency and number of basidiocarps observed in China have been declining significantly. So far, however, we know little about the species diversity of Hydnellum in China. In this study, we conducted molecular phylogenetic analyses based on sections of multiple loci, including the large subunit of nuclear ribosomal RNA gene (nLSU), the internal transcribed spacer regions (ITS), the small subunit of nuclear ribosomal RNA gene (SSU) and the second-largest subunit of RNA polymerase II gene (RPB2), as well as morphological studies, of collected samples of Hydnellum from China. We also inferred Maximum Likelihood and Bayesian phylogenies for the order Thelephorales from the dataset of the combined nLSU and ITS. This study has revealed the phylogenetic position of Hydnellum in the order Thelephorales, and phylogenetically confirmed ten major clades in Thelephorales; Twenty-nine taxa are proposed, described or reported, including 10 new subgenera (Hydnellum subgenus Hydnellum, subg. Caesispinosum, subg. Croceum, subg. Inflatum, subg. Rhizomorphum, subg. Scabrosum, subg. Spongiosum, subg. Subindufibulatum, subg. Violaceum and subg. Zonatum), 11 new species (Hydnellum atrorubrum, H. atrospinosum, H. bomiense, H. brunneorubrum, H. fibulatum, H. granulosum, H. inflatum, H. rubidofuscum, H. squamulosum, H. sulcatum and H. yunnanense), 3 newly recorded species (H. caeruleum, H. peckii and H. spongiosipes) and 5 notable specimens (Hydnellum sp 1, H. sp 2, H. sp 3, H. sp 4 and H. sp 5). A classification system based on the morphological characteristics (especially the hyphal structure types) and molecular analyses is proposed to accommodate most species in Hydnellum. The distinguishing characters of the subgenera and the new species with their closely related taxa are discussed. A key to the species of Hydnellum from China is provided.

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Morphological and molecular data on a new species of Plagiorhynchus Lühe, 1911 (Acanthocephala: Plagiorhynchidae) from the long-billed curlew (Numenius americanus) from northern Mexico

Journal of Helminthology ◽

10.1017/s0022149x19000543 ◽

2019 ◽

Vol 94 ◽

Cited By ~ 3

Author(s):

M. García-Varela ◽

J.-K. Park ◽

J.S. Hernández-Orts ◽

C.D. Pinacho-Pinacho

Keyword(s):

New Species ◽

Marine Mammals ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Molecular Data ◽

Small Subunit ◽

Nuclear Ribosomal Dna ◽

Northern Mexico ◽

Numenius Americanus ◽

A New Species

Abstract A new species of the genus Plagiorhynchus Lühe, 1911 from the intestine of the long-billed curlew (Numenius americanus) from northern Mexico is described. Plagiorhynchus (Plagiorhynchus) aznari n. sp. is morphologically distinguished from other congeneric species from the Americas by having a trunk expanded anteriorly and a cylindrical proboscis, armed with 19 longitudinal rows of hooks, with 14–15 hooks each row. Nearly complete sequences of the small subunit and large subunit of the nuclear ribosomal DNA of the new species were determined and compared with available sequences from GenBank. Phylogenetic analyses inferred from the two molecular markers consistently showed that P. (Plagiorhynchus) aznari n. sp. is closely related to P. (Plagiorhynchus) allisonae, and this clade is sister to a clade formed by P. (Prosthorhynchus) transversus and P. (Prosthorhynchus) cylindraceus from Plagiorhynchidae. The new species represents the second record of the genus in Mexico and the fourth species in the Americas. The phylogenetic relationships among the members of the order Polymorphida in this study provide significant insights into the evolution of ecological associations between parasites and their definitive hosts. Our analyses suggest that the colonization of marine mammals, fish-eating birds and waterfowl in Polymorphidae might have occurred independently, from a common ancestor of Centrorhynchidae and Plagiorhynchidae that colonized terrestrial birds and mammals.

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Dothidea kunmingensis, a novel asexual species of Dothideaceae on Jasminum nudiflorum (winter jasmine) from Southwestern China

Phytotaxa ◽

10.11646/phytotaxa.529.1.3 ◽

2021 ◽

Vol 529 (1) ◽

pp. 43-56

Author(s):

YING GAO ◽

JUTAMART MONKAI ◽

ELENI GENTEKAKI ◽

GUANG-CONG REN ◽

DHANUSHKA N. WANASINGHE ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Small Subunit ◽

Morphological Characterization ◽

Small Subunit Rrna ◽

Sister Relationship ◽

Large Subunit Rrna

During a survey of saprobic microfungi in Southwest China, a coelomycetous fungus was found on dead twigs of Jasminum nudiflorum in Kunming, Yunnan Province. Based on a detailed morphological characterization coupled with multi-locus phylogenetic analyses, the fungus was identified as a new species in the genus Dothidea. Phylogenetic analyses using a combined matrix consisting of internal transcribed spacer (ITS), large subunit rRNA (LSU), small subunit rRNA (SSU), beta tubulin (tub2) and translation elongation factor-1 alpha (tef1-α) confirmed its placement in Dothideaceae and revealed a sister relationship to Dothidea eucalypti. The new species is characterized by pycnidial conidiomata, ampulliform or doliiform conidiogenous cells as well as aseptate, subglobose to ovoid, hyaline to pale-brown conidia. Comprehensive descriptions and illustrations are provided. Morphological characteristics of asexual morph taxa in Dothideaceae are also summarized and discussed.

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Unveiling the Hidden Diversity of Rock-Inhabiting Fungi: Chaetothyriales from China

Journal of Fungi ◽

10.3390/jof6040187 ◽

2020 ◽

Vol 6 (4) ◽

pp. 187

Author(s):

Wei Sun ◽

Lei Su ◽

Shun Yang ◽

Jingzu Sun ◽

Bingjie Liu ◽

...

Keyword(s):

New Species ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Morphological Characteristics ◽

Small Subunit ◽

Internal Transcribed Spacer Region ◽

Natural Rock ◽

Rock Inhabiting Fungi

Rock-inhabiting fungi (RIF) are nonlichenized fungi that naturally colonize rock surfaces and subsurfaces. The extremely slow growth rate and lack of distinguishing morphological characteristics of RIF resulted in a poor understanding on their biodiversity. In this study, we surveyed RIF colonizing historical stone monuments and natural rock formations from throughout China. Among over 1000 isolates, after preliminary delimitation using the internal transcribed spacer region (ITS) sequences, representative isolates belonging to Trichomeriaceae and Herpotrichiellaceae were selected for a combined analysis of ITS and the nuclear ribosomal large subunit (nucLSU) to determine the generic placements. Eight clades representing seven known genera and one new genus herein named as Anthracina were placed in Trichomeriaceae. While, for Herpotrichiellaceae, two clades corresponded to two genera: Cladophialophora and Exophiala. Fine-scale phylogenetic analyses using combined sequences of the partial actin gene (ACT), ITS, mitochondrial small subunit ribosomal DNA (mtSSU), nucLSU, the largest subunit of RNA polymerase II (RPB1), small subunit of nuclear ribosomal RNA gene (SSU), translation elongation factor (TEF), and β-tubulin gene (TUB) revealed that these strains represented 11 and 6 new species, respectively, in Trichomeriaceae and Herpotrichiellaceae. The 17 new species were described, illustrated for their morphologies and compared with similar taxa. Our study demonstrated that the diversity of RIF is surprisingly high and still poorly understood. In addition, a rapid strategy for classifying RIF was proposed to determine the generic and familial placements through preliminary ITS and nucLSU analyses, followed by combined analyses of five loci selected from ACT, ITS, mtSSU, nucLSU, RPB1, and/or the second subunit of RNA polymerase II gene (RPB2), SSU, TEF, and TUB regions to classify RIF to the species level.

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