DNA barcoding and integrative taxonomy of Macrobiotus hufelandi C.A.S. Schultze 1834, the first tardigrade species to be described, and some related species

Zootaxa ◽  
2011 ◽  
Vol 2997 (1) ◽  
pp. 19 ◽  
Author(s):  
ROBERTO BERTOLANI ◽  
LORENA REBECCHI ◽  
ILARIA GIOVANNINI ◽  
MICHELE CESARI
Keyword(s):  
Dna Barcoding ◽  
Integrative Taxonomy ◽  
Genetic Distances ◽  
Type Locality ◽  
Morphological Data ◽  
New Taxon ◽  
Black Forest ◽  
Mt Dna ◽  
Additional Material ◽  
Starting Point

Within the framework of a DNA barcoding project on tardigrade species, a study was carried out on Macrobiotus hufelandi C.A.S. Schultze 1834, the first formally described tardigrade species. We used samples collected from the type locality and additional material from other European sites containing species of the “M. hufelandi group”. The study was performed by integrating morphological, karyological and molecular (mt-DNA cox1) information and comparing these data with morphological data from the type material. Several species from this group were found in the type locality of M. hufelandi (near Freiburg, Black Forest, Germany) and these were all barcoded. One was M. hufelandi, the other two were: Macrobiotus sandrae Bertolani & Rebecchi 1993 (originally described from the same locality), and Macrobiotus vladimiri Bertolani, Biserov, Rebecchi & Cesari in press (type locality Andalo, Italy), all with interspecific genetic distances of more than 19%. A fourth cryptic species, which had the same morphology as M. hufelandi but a genetic distance of 6.7%, was not described as a new taxon but named M. cf. hufelandi sp.1 for this study. Macrobiotus sandrae and M. vladimiri were also present (and barcoded) in Italy (Alps). Additional individuals (animals and eggs) were also found, and barcoded, in Italy (Apennines) and Switzerland that belonged to the haplogroup Macrobiotus cf. hufelandi sp. 1. These data together with other recent studies on tardigrade DNA barcoding represent a starting point for further studies on tardigrade biogeography, phylogeography and diversity.

The middle lower Cambrian (Ovetian)Lunagraulosn. gen. from Spain and the oldest trilobite records

2015 ◽  
Vol 152 (6) ◽  
pp. 1123-1136 ◽  
Author(s):  
ELADIO LIÑÁN ◽  
JOSÉ ANTONIO GÁMEZ VINTANED ◽  
RODOLFO GOZALO
Keyword(s):  
Fossil Record ◽  
Lower Cambrian ◽  
New Genus ◽  
Type Locality ◽  
New Taxon ◽  
Northern Spain ◽  
Central Spain ◽  
Additional Material ◽  
The World ◽  
The Village

AbstractThe type material ofAgraulos antiquusSdzuy, 1961 from the La Herrería Formation, northern Spain, is revised together with additional material and included in the new genusLunagraulos. The stratigraphical range ofLunagraulos antiquus(Sdzuy, 1961) – occurring below that of the trilobite species of the generaLunolenus,MetadoxidesandDolerolenusin the type locality of Los Barrios de Luna in the province of León, northern Spain – and the accompanying ichnofossil assemblage demonstrate an Ovetian age (lower part of Cambrian Stage 3, currently being discussed by the International Subcommission on Cambrian Stratigraphy) for this species. Moreover, the trilobiteLunagraulos tamamensisn. gen. n. sp. is found in the Tamames Sandstone near the village of La Rinconada in the province of Salamanca, central Spain. The biostratigraphical position of this new taxon and its accompanying ichnoassemblage is also analysed and assigned to the lowermost Ovetian Stage. The genusLunagraulosis therefore the oldest agraulid found in the fossil record. The exceptional presence ofLunagraulosin a marine coarse siliciclastic succession – a facies rather typical for the ichnofossilsCruzianaandRusophycus, some of the oldest signs of trilobite activity – suggests that first trilobite representatives may have inhabited high- to middle-energy, marine environments. This hypothesis may also explain both the taxonomic and biostratigraphic heterogeneity of the first trilobite genera appearing across the world, due to preservation problems in this type of facies. Comparison of theLunagraulos biostratigraphy with other coeval Spanish fossil assemblages allows us to propose its intercontinental correlation with the oldest records of currently known trilobites.

The bee Ruizanthedella mutabilis Spinola (Hymenoptera: Halictidae): a very common but poorly known species studied using integrative taxonomy

Zootaxa ◽  
2019 ◽  
Vol 4563 (1) ◽  
pp. 191
Author(s):  
ROCÍO A. GONZÁLEZ-VAQUERO ◽  
ARTURO ROIG-ALSINA
Keyword(s):  
Dna Barcoding ◽  
New Records ◽  
Atlantic Coast ◽  
Abundant Species ◽  
Integrative Taxonomy ◽  
Junior Synonym ◽  
Morphological Data ◽  
Valid Species ◽  
The Andes ◽  
Argentinean Patagonia

Ruizanthedella mutabilis (Spinola) is a very abundant species in Chile and the northwest of Argentinean Patagonia. In this contribution, Halictus nigrocaeruleus Spinola 1851 is established as a junior synonym of R. mutabilis (Spinola 1851), after considering morphological data, DNA barcoding results, and biological observations. The variability in the colouration of the metasoma has been incorrectly used to distinguish these colour forms as valid species. New records enlarge the distribution of the species in Argentina, from the Andes to the Atlantic coast. 

An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens

Zootaxa ◽  
2011 ◽  
Vol 3104 (1) ◽  
pp. 42 ◽  
Author(s):  
MICHELE CESARI ◽  
ILARIA GIOVANNINI ◽  
ROBERTO BERTOLANI ◽  
LORENA REBECCHI
Keyword(s):  
Dna Barcoding ◽  
Dna Barcode ◽  
Intraspecific Variability ◽  
Original Description ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Type Locality ◽  
Moss Sample ◽  
Voucher Specimens

We have in recent papers revealed that an integrative taxonomy approach helps to solve taxonomic problems in tardigrades. However, whole tardigrades are required for DNA work, which leaves no hologenophore voucher specimens with adult morphology. Using a novel methodology for the Tardigrada, we introduce the practice of collecting high quality maximum magnification light microscopy images of recently thawed animals to act as hologenophore voucher specimens of animals later used for DNA barcode sequencing. Within the framework of a DNA barcoding project on tardigrades, we collected a moss sample from the type locality of Macrobiotus terminalis Bertolani & Rebecchi, 1993 (Castelsantangelo, Central Apennines, Italy), a species of the “Macrobiotus hufelandi group”. Within the moss sample we found several animals and eggs with a morphology that corresponded to the original description of M. terminalis, while others were attributable to Macrobiotus macrocalix Bertolani & Rebecchi, 1993. In this study, molecular (cox1 mtDNA) analyses demonstrated no intraspecific variability in M. terminalis from the type locality but very large interspecific differences when compared with M. macrocalix and GenBank data for other species within the M. “hufelandi group”. There was also a large difference between our M. terminalis sequences and the GenBank data of a specimen attributed to the same species. The GenBank sequence originated from a population in the Northern Apennines, whose morphology appeared to be like that of the specimens of the locus typicus. This confirmed the importance in utilising material from the type locality for linking molecular data to the species’ morphological characters. Our paper underlines the importance of an integrative taxonomy in species diagnoses and demonstrates a scenario where morphological observations alone are not always sufficient. Lastly, this work adds reliable information to the sequence reference library that provides a useful building block for further studies on similar and related tardigrade taxa.

scholarly journals A new record of odd-scaled snake (Serpentes, Xenodermidae) from Vietnam: expanded description of Parafimbrios vietnamensis based on integrative taxonomy

ZooKeys ◽  
2021 ◽  
Vol 1048 ◽  
pp. 79-89
Author(s):  
Nikolai L. Orlov ◽  
Oleg A. Ermakov ◽  
Tao Thien Nguyen ◽  
Natalia B. Ananjeva
Keyword(s):  
Genetic Divergence ◽  
New Record ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Type Locality ◽  
Morphological Data ◽  
Snake Species ◽  
Male Individual

Based on the combination of molecular and morphological data, we herein report the second known finding of the xenodermid snake species Parafimbrios vietnamensis Ziegler, Ngo, Pham, Nguyen, Le & Nguyen, 2018. The male individual was found in the Yen Bai Province of northwestern Vietnam, more than 200 km from the type locality in Lai Chau Province. Genetic divergence between the newly-collected male and the holotype was low (1.7%), and is in agreement with morphological data that supports that they are conspecific. We give a detailed description of the morphological characters and coloration of the new record and provide an expanded diagnosis of P. vietnamensis. Parafimbrios is a poorly-understood genus, and our recent discovery brings the total number of known specimens of the genus to nine, 1/3 of them having been found in Vietnam (one specimen of P. lao and now two specimens of P. vietnamensis).

scholarly journals Accelerating the knowledge of Peruvian Chalcididae (Insecta,  Hymenoptera, Chalcidoidea) with integrative taxonomy

2019 ◽  
Vol 7 ◽  
Author(s):  
Bruno Cancian de Araujo ◽  
Marcelo Tavares ◽  
Thales Renan Brotto ◽  
Juliana Silva-Freitas ◽  
Max Estefani Santos ◽  
...  
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Dna Sequences ◽  
Fold Increase ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Peruvian Amazon ◽  
Insect Biodiversity ◽  
Canopy Fogging ◽  
Molecular Dataset

We present the first regional inventory of the fauna of Chalcididae in the Peruvian Amazon, with a nearly 6-fold increase in the number of species recorded for the country. A total of 418 specimens of Chalcididae were collected between 2000 and 2017 at the Panguana Reserve, Peruvian Amazon, 400 of which were obtained using Malaise traps and the remaining 18 specimens by canopy fogging. The morphological analyses indicated that these specimens represent 183 species of Chalcididae in 10 different genera, with 173 new to Peru and 134 potentially new species. We submitted 268 specimens, representing 167 species, to DNA barcoding. Of these, 141 specimens yielded sequences, 136 of them with a minimum of 300 bp. Sixty specimens were assigned a BIN by the Barcode of Life Database System (BOLD), resulting in 50 BINs. A cluster analysis of 138 individuals that yielded DNA sequences longer than 100 bp revealed 118 MOTUs (molecular operative taxonomic units), all of them highly congruent with the morphological data. Prior to the present study, 37 species in 9 genera of Chalcididae were known from Peru. With our results, this number was increased to 210 species in 13 genera. The present study is the result of a joint effort between the SNSB - Zoologische Staatssammlung München, Germany (ZSM) and the Insect Biodiversity Laboratory of the Universidade Federal do Espírito Santo, Vitória, Brazil (LaBI-UFES), intending to apply an accelerated taxonomic treatment of the Chalcididae of the Panguana reserve using traditional morphological approaches in combination with DNA barcoding. The complete molecular dataset and associated voucher information is publicly available through BOLD. The new species that were discovered as part of the study are being formally described elsewhere as part of taxonomic treatments of Neotropical and world generic revisions at LaBI-UFES.

DNA Barcoding of Chinese species of the genus Eurydema Laporte, 1833 (Hemiptera: Pentatomidae)

Zootaxa ◽  
2017 ◽  
Vol 4286 (2) ◽  
pp. 151 ◽  
Author(s):  
WANQING ZHAO ◽  
QING ZHAO ◽  
MIN LI ◽  
JIUFENG WEI ◽  
XIANHONG ZHANG ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Genetic Distances ◽  
Morphological Data ◽  
New Combination ◽  
Coi Gene ◽  
Distinct Cluster ◽  
Variation Patterns ◽  
Nj Tree ◽  
Interspecific Distance ◽  
Coi Sequences

The phenotype, as a significant identification character for the bug species Eurydema Laporte, 1833, possesses many variation patterns, making this species difficult to determine. In this context, DNA barcoding provides an efficient method for species identification. In our study, we used distance-based and tree-based methods to assess the effectiveness of COI gene as an additional taxonomic method. We examined COI sequences of 203 specimens collected in China. Intra- and interspecific genetic distances were calculated, and a phylogenetic tree was constructed using the neighbor-joining (NJ) method. The program TaxonDNA was used to calculate the distribution of distances and investigated the barcode gap. DNA barcodes revealed 100% (ABGD), 99.5% (TaxonDNA) and 97.5% (Barcode Index Numbers) successful identification rates. In the NJ tree, all taxonomic species showed monophyletic clusters that were separated from each other by large genetic distances (> 4.3%). Low divergence (< 0.6%) and non-monophyly clusters that included different phenotype variations were found in E. dominulus and E. oleracea. Compared with other Eurydema species, E. sp. had an average interspecific distance of 7.4% that was congeneric distance, and formed a distinct cluster among Eurydema tree clades. We also confirmed a new combination of Eurydema qinlingensis (Zheng, 1982) comb. nov. based on DNA barcoding and morphology data. Overall, our data showed that, despite the little barcode overlap between intra- and interspecific genetic distances, DNA barcoding is a useful and effective method for enhancing morphological data and resolving taxonomic problems. 

Molecular phylogeny and taxonomic evaluation of the genus Asaccus Dixon and Anderson, 1973 (Reptilia: Phyllodactylidae) in Iran

2020 ◽  
pp. 207-214
Author(s):  
Akbar Fattahi
Keyword(s):  
Molecular Phylogeny ◽  
Nuclear Gene ◽  
Genetic Distances ◽  
Genetic Lineage ◽  
New Taxon ◽  
Zagros Mountains ◽  
Relict Species ◽  
Taxonomical Revision ◽  
Two Populations ◽  
Taxonomic Evaluation

The Iranian species of the phyllodactylid geckos of the genus Asaccus are found only in the valleys of the Zagros Mountains, a region which represents an important area of endemism in western Iran. Recently, many relict species have been described from the central and southern parts of the Zagros Mountains, which were previously known as A. elisae. The recent descriptions of species within this complex suggest that diversity within the genus may be higher than expected and that its taxonomy and systematics should be revised. In the present study, phylogenetic relationships within the genus Asaccus were evaluated using two mitochondrial and one nuclear gene. Genetically, the genus shows high levels of variability. The molecular phylogeny of the genus suggests the presence of three main clades along the Zagros Mountains with the southern population (from the Hormozgan province) and one clade (A. sp8 and A. sp9) being sister taxon to A. montanus from UAE. The remaining samples are separated into two reciprocally monophyletic groups: the northern (Kurdistan, Kermanshah and Ilam provinces) and the central (Lorestan, Khuzestan, Kohgilouye-Bouyer Ahmad and Fars provinces) Zagros groups. The results of the present study suggest that populations attributed to A. elisae in Iran correspond to distinct lineages with high genetic distances. In brief, our results suggest that the genus needs a major taxonomical revision The Arabian origin of the genus has not been confirmed, because two populations from Zagros were located within the A. montanus, A. gallagheri and A. platyrhynchus clade. Further morphological analyses are needed to systematically define each genetic lineage as a new taxon.

scholarly journals Unexpected larger distribution of paleogene stem-rollers (AVES, CORACII): new evidence from the Eocene of Patagonia, Argentina

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Federico J. Degrange ◽  
Diego Pol ◽  
Pablo Puerta ◽  
Peter Wilf
Keyword(s):  
Hind Limb ◽  
Divergence Time ◽  
First Record ◽  
Morphological Data ◽  
New Taxon ◽  
Early Eocene ◽  
Important Data ◽  
New Material ◽  
Holarctic Region ◽  
New Evidence

AbstractHere we present the first record of a stem-Coracii outside the Holarctic region, found in the early Eocene of Patagonia at the Laguna del Hunco locality. Ueekenkcoracias tambussiae gen. et sp. nov. consists of an incomplete right hind limb that presents the following combination of characters, characteristic of Coracii: relatively short and stout tibiotarsus, poorly developed crista cnemialis cranialis, short and wide tarsometatarsus, with the tuberositas m. tibialis cranialis located medially on the shaft, and curved and stout ungual phalanges. Although the presence of a rounded and conspicuous foramen vasculare distale and the trochlea metatarsi II strongly deflected medially resemble Primobucconidae, a fossil group only found in the Eocene of Europe and North America, our phylogenetic analysis indicates the new taxon is the basalmost known Coracii. The unexpected presence of a stem-Coracii in the Eocene of South America indicates that this clade had a more widespread distribution than previously hypothesized, already extending into the Southern Hemisphere by the early Eocene. Ueekenkcoracias tambussiae represents new evidence of the increasing diversity of stem lineages of birds in the Eocene. The new material provides novel morphological data for understanding the evolutionary origin and radiation of rollers and important data for estimates of the divergence time of the group.

DNA barcoding of bats (Chiroptera) from the Colombian northern region

Mammalia ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Álvaro J. Benítez ◽  
Dina Ricardo-Caldera ◽  
María Atencia-Pineda ◽  
Jesús Ballesteros-Correa ◽  
Julio Chacón-Pacheco ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Dna Sequences ◽  
Genetic Distances ◽  
Northern Region ◽  
Coi Gene ◽  
Individual Species ◽  
Species Variation ◽  
Molossus Molossus ◽  
Artibeus Lituratus

Abstract Bats are mammals of great ecological and medical importance, which have associations with different pathogenic microorganisms. DNA barcoding is a tool that can expedite species identification using short DNA sequences. In this study, we assess the DNA barcoding methodology in bats from the Colombian Northern region, specifically in the Córdoba department. Cytochrome oxidase subunit I (COI) gene sequences of nine bat species were typified, and their comparison with other Neotropic samples revealed that this marker is suitable for individual species identification, with ranges of intra-species variation from 0.1 to 0.9%. Bat species clusters are well supported and differentiated, showing average genetic distances ranging from 3% between Artibeus lituratus and Artibeus planirostris, up to 27% between Carollia castanea and Molossus molossus. C. castanea and Glossophaga soricina show geographical structuring in the Neotropic. The findings reported in this study confirm DNA barcoding usefulness for fast species identification of bats in the region.

Euphorbia mongoliensis (Euphorbiaceae), a new species from Inner Mongolia, China

Phytotaxa ◽  
2021 ◽  
Vol 501 (1) ◽  
pp. 151-161
Author(s):  
ER-HUAN ZANG ◽  
MING-XU ZHANG ◽  
WEN-LE WANG ◽  
CHUN-HONG ZHANG ◽  
MIN-HUI LI
Keyword(s):  
New Species ◽  
Geographical Distribution ◽  
Inner Mongolia ◽  
Pollen Morphology ◽  
Morphological Characteristics ◽  
Euphorbia Esula ◽  
Morphological Data ◽  
New Taxon ◽  
Flora Of China ◽  
A New Species

In May 2020, a new taxon of Euphorbia, Euphorbiaceae was collected from a dry hillside of Dongsheng District, Ordos City, Inner Mongolia. The morphological characteristics of the specimens analyzed differ from those of the known Euphorbia species from this region; therefore, we suspected this may be a new species, and we set to analyze the ITS2 sequences of some Euphorbia species. The results show that the new taxon belongs to the sect. Esula of Euphorbia subg. Esula. It is similar to Euphorbia esula (description from Flora of China) but does not belong to the same species. Concomitantly, plant morphological data and pollen morphology results show significant differences between the new taxon, E. esula and E. caesia, a finding that supports the delimitation of this new taxon, which is named Euphorbia mongoliensis in accordance with its geographical distribution.

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