The bee Ruizanthedella mutabilis Spinola (Hymenoptera: Halictidae): a very common but poorly known species studied using integrative taxonomy

Zootaxa ◽  
2019 ◽  
Vol 4563 (1) ◽  
pp. 191
Author(s):  
ROCÍO A. GONZÁLEZ-VAQUERO ◽  
ARTURO ROIG-ALSINA
Keyword(s):  
Dna Barcoding ◽  
New Records ◽  
Atlantic Coast ◽  
Abundant Species ◽  
Integrative Taxonomy ◽  
Junior Synonym ◽  
Morphological Data ◽  
Valid Species ◽  
The Andes ◽  
Argentinean Patagonia

Ruizanthedella mutabilis (Spinola) is a very abundant species in Chile and the northwest of Argentinean Patagonia. In this contribution, Halictus nigrocaeruleus Spinola 1851 is established as a junior synonym of R. mutabilis (Spinola 1851), after considering morphological data, DNA barcoding results, and biological observations. The variability in the colouration of the metasoma has been incorrectly used to distinguish these colour forms as valid species. New records enlarge the distribution of the species in Argentina, from the Andes to the Atlantic coast. 

Uncovering Neotropical treefrog diversity: integrative taxonomy reveal paraphyly in Boana atlantica (Amphibia, Anura, Hylidae)

2019 ◽  
Vol 40 (4) ◽  
pp. 511-521
Author(s):  
Luana Rodrigues Lima ◽  
Marcos Jorge Matias Dubeux ◽  
Filipe Augusto Cavalcanti do Nascimento ◽  
Daniel Pacheco Bruschi ◽  
Tamí Mott
Keyword(s):  
Strong Support ◽  
Taxonomic Status ◽  
Nucleolar Organizer ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Valid Species ◽  
Nucleolar Organizer Regions ◽  
Candidate Species ◽  
Species Being

Abstract Boana atlantica was described based on morphological characters to acommodate populations from Bahia state, Brazil, previously referred to as B. punctata. However, its taxonomic validity is questionable due to inconsistencies in bioacoustic data. Here, we integrate molecular, cytogenetic and morphological data to re-evaluate the taxonomic status of B. atlantica. Molecular analyses provided strong support for the hypothesis that B. atlantica is a valid species, being, however, nominal populations from Bahia state paraphyletic with respect to individuals of B. atlantica collected in Alagoas and Pernambuco states. These populations shown high genetic divergence in the 16S rRNA mitochondrial fragment, and we consider populations from Alagoas and Pernambuco as a putative new candidate species of Boana punctata group. The external and internal morphology of the tadpoles of these lineages were similar, but Nucleolar Organizer Regions (NOR) was sited in different chromosomes. Our results indicate that B. atlantica is paraphyletic and may be a complex of species. Morphological of adult and/or bioacoustical data should be further assessed to find diagnostic characters to tease these lineages apart and name them properly.

DNA barcoding and integrative taxonomy of Macrobiotus hufelandi C.A.S. Schultze 1834, the first tardigrade species to be described, and some related species

Zootaxa ◽  
2011 ◽  
Vol 2997 (1) ◽  
pp. 19 ◽  
Author(s):  
ROBERTO BERTOLANI ◽  
LORENA REBECCHI ◽  
ILARIA GIOVANNINI ◽  
MICHELE CESARI
Keyword(s):  
Dna Barcoding ◽  
Integrative Taxonomy ◽  
Genetic Distances ◽  
Type Locality ◽  
Morphological Data ◽  
New Taxon ◽  
Black Forest ◽  
Mt Dna ◽  
Additional Material ◽  
Starting Point

Within the framework of a DNA barcoding project on tardigrade species, a study was carried out on Macrobiotus hufelandi C.A.S. Schultze 1834, the first formally described tardigrade species. We used samples collected from the type locality and additional material from other European sites containing species of the “M. hufelandi group”. The study was performed by integrating morphological, karyological and molecular (mt-DNA cox1) information and comparing these data with morphological data from the type material. Several species from this group were found in the type locality of M. hufelandi (near Freiburg, Black Forest, Germany) and these were all barcoded. One was M. hufelandi, the other two were: Macrobiotus sandrae Bertolani & Rebecchi 1993 (originally described from the same locality), and Macrobiotus vladimiri Bertolani, Biserov, Rebecchi & Cesari in press (type locality Andalo, Italy), all with interspecific genetic distances of more than 19%. A fourth cryptic species, which had the same morphology as M. hufelandi but a genetic distance of 6.7%, was not described as a new taxon but named M. cf. hufelandi sp.1 for this study. Macrobiotus sandrae and M. vladimiri were also present (and barcoded) in Italy (Alps). Additional individuals (animals and eggs) were also found, and barcoded, in Italy (Apennines) and Switzerland that belonged to the haplogroup Macrobiotus cf. hufelandi sp. 1. These data together with other recent studies on tardigrade DNA barcoding represent a starting point for further studies on tardigrade biogeography, phylogeography and diversity.

scholarly journals Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

2021 ◽  
Vol 19 (2) ◽  
Author(s):  
Karen L. A. Guimarães ◽  
Juan J. Rosso ◽  
Mendelsohn F. B. Souza ◽  
Juan M. Díaz de Astarloa ◽  
Luís R. R. Rodrigues
Keyword(s):  
Dna Barcoding ◽  
Amazon Basin ◽  
First Record ◽  
Morphological Data ◽  
Disjunct Distribution ◽  
Valid Species ◽  
Integrative Approach ◽  
Molecular Evidence ◽  
La Plata ◽  
La Plata Basin

Abstract The Hoplias malabaricus group encompasses six valid species and still is believed to harbors cryptic diversity. In this work, an integrative approach including morphological, DNA barcoding, and cytogenetic considerations was conducted to characterize a population of H. malabaricus from the Amazon basin that was recently allocated in the same mitochondrial lineage with H. misionera, a species originally described from La Plata basin. The DNA barcoding analysis revealed that the Amazon population nested together with H. misionera specimens from the La Plata basin (BIN AAB1732) in the same cluster. The intragroup distance (0.5%) was 12 times lower than the nearest neighbor (6%) distance. The morphometric analysis demonstrated slightly variation between Amazon and La Plata populations, being the former composed by larger specimens. Further morphological data supported the molecular evidence of H. misionera inhabiting Amazon basin. The karyotype characterization of H. misionera in the Amazon population showed 2n=40 and karyotypic formulae 20m+20sm, that added to C-banding, Ag-NOR and 18S results are suggestive of the similarity to karyomorph C of H. malabaricus. This work reveals the first record of H. misionera outside of La Plata basin and expands the species distribution for 2500 km northward until the Marajó Island, estuary of Amazonas River.

Contribution to the Ophioninae (Hymenoptera: Ichneumonidae) of Iran with the description of 16 new species and an illustrated key to the Eremotylus of the Western Palaearctic

Zootaxa ◽  
2021 ◽  
Vol 5023 (2) ◽  
pp. 151-206
Author(s):  
NIKLAS JOHANSSON ◽  
ALI AMERI ◽  
MATTHIAS RIEDEL ◽  
ALI ASGHAR TALEBI ◽  
EBRAHIM EBRAHIMI
Keyword(s):  
New Species ◽  
New Records ◽  
Junior Synonym ◽  
Valid Species ◽  
Additional Species ◽  
Study Material ◽  
Western Palaearctic

In this study, material of Iranian Ophioninae was examined. In total, 63 species are recognized as occurring in Iran. Sixteen species are described as new to science: Enicospilus amerii Johansson sp. nov., E. apocalypticus Johansson sp. nov., E. haladai Johansson sp. nov., Enicospilus peri sp. nov., Ophion al Johansson sp. nov., Ophion angustigena Johansson sp. nov., Ophion breviflagellator Johansson sp. nov., Ophion golestanicus Johansson sp. nov., Ophion klimenkoi Johansson sp. nov., Ophion persicus Johansson sp. nov., Ophion schmidti Johansson sp. nov., Ophion sinuosus Johansson sp. nov., Ophion sistanicus Johansson sp. nov., Ophion transcarinatus Johansson sp. nov., Ophion vasi Johansson sp. nov. and Ophion xrafstra Johansson sp. nov. Sixteen species: Enicospilus combustus (Gravenhorst, 1829); E. intermedius (Johansson, 2018); E. undulatus (Gravenhorst, 1829); Ophion angularis Johansson & Cederberg, 2019; Ophion bipictor Aubert, 1980; Ophion crassicornis Brock, 1982; Ophion confusus Johansson, 2019; Ophion ellenae Johansson, 2019; Ophion eremita Kokujev, 1906; Ophion kallanderi Johansson, 2019; Ophion rostralis Meyer, 1935; Ophion rufoniger Kohl, 1905; Ophion semipullatus Kokujev, 1909; Ophion tenuicornis Johansson, 2019; Hellwigiella dichromoptera (Costa, 1886) and Eremotylus curvinervis (Kriechbaumer, 1878) are new records for the fauna of Iran. Enicospilus flavocephalus (Kirby, 1900), Enicospilus monostigma (Vollenhoven, 1879) and Eremotylus sibiricus Szépligeti, 1905 are excluded from the Iranian checklist due to previous misidentifications. Enicospilus tarsalis Hedwig, 1957 syn. nov. is excluded from synonymy with E. ahngeri and instead introduced as a junior synonym of E. cruciator Viktorov, 1957. Enicospilus affinis Hedwig, 1957 stat. rev. is excluded from synonymy with E. ocellatus Shestakov, 1926 and reinstated as a valid species. Enicospilus splendidus Rousse, Soliman & Gadallah, 2017 syn. nov. is a junior synonym of Enicospilus stenopsis Kohl, 1905. Enicospilus grandiflavus Townes & Townes, 1973 (= E. grandis Morley) syn. nov. is synonymized with Enicospilus unicallosus (Vollenhoven, 1878). Enicospilus contributus Shestakov, 1926 is confirmed as a junior synonym of E. merdarius (Gravenhorst, 1829). Ophion fossulatus Hedwig, 1957 stat. rev. is excluded from synonymy with Ophion mocsaryi Brauns, 1899 and reinstated as a valid species close to Ophion parvulus Kriechbaumer. Ophion mocsaryi var. decedens Hedwig, 1961 syn. nov. is a synonym of Ophion bipictor Aubert, 1980. Ophion obscuratus var. heratensis Hedwig, 1961 syn. nov. is treated as a synonym of Ophion turcomanicus Szépligeti, 1905. Ophion pravinervis Kokujev, 1906, previously only known from the lost type collected in Georgia, is redescribed. Despite the relatively high number of new species here presented, the Iranian fauna most likely still harbours additional species awaiting discovery.  

A new species of Pothea, with new records and taxonomic notes on other species of the genus (Hemiptera: Heteroptera: Reduviidae)

Zootaxa ◽  
2020 ◽  
Vol 4778 (3) ◽  
pp. 439-470
Author(s):  
HÉLCIO R. GIL-SANTANA
Keyword(s):  
New Species ◽  
French Guiana ◽  
New Records ◽  
Junior Synonym ◽  
Color Variation ◽  
Valid Species ◽  
Type Specimens ◽  
Males And Females ◽  
First Time ◽  
A New Species

Pothea carpinteroi sp. nov. (Hemiptera: Heteroptera: Reduviidae: Ectrichodiinae) is described based on two male specimens from Mexico. Pothea aeneonitens Stål, 1864 and P. lugens (Fabricius, 1803) are recorded from French Guiana for the first time. Taxonomical notes on these latter two species and P. haematogaster Breddin, 1903 and P. sanguiniventris Miller, 1956 are also presented. A lectotype is designated for P. lugens. Pothea centralis Walker, 1873, treated as a junior synonym of P. lugens by previous authors, is regarded as a potentially valid species. Color variation in the sternites of males and females of P. frontalis (Lepeletier & Serville, 1825) are recorded for the first time. Photographs of the type specimens of P. aeneonitens, P. bivittata Champion, 1899, P. centralis, P. frontalis, P. haematogaster, P. halffteri Carpintero, 1980, P. lugens, P. maculata Champion, 1899, P. reyesi Carpintero, 1980, P. sanguiniventris and P. venatrix Hussey, 1953, are presented. 

scholarly journals Accelerating the knowledge of Peruvian Chalcididae (Insecta,  Hymenoptera, Chalcidoidea) with integrative taxonomy

2019 ◽  
Vol 7 ◽  
Author(s):  
Bruno Cancian de Araujo ◽  
Marcelo Tavares ◽  
Thales Renan Brotto ◽  
Juliana Silva-Freitas ◽  
Max Estefani Santos ◽  
...  
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Dna Sequences ◽  
Fold Increase ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Peruvian Amazon ◽  
Insect Biodiversity ◽  
Canopy Fogging ◽  
Molecular Dataset

We present the first regional inventory of the fauna of Chalcididae in the Peruvian Amazon, with a nearly 6-fold increase in the number of species recorded for the country. A total of 418 specimens of Chalcididae were collected between 2000 and 2017 at the Panguana Reserve, Peruvian Amazon, 400 of which were obtained using Malaise traps and the remaining 18 specimens by canopy fogging. The morphological analyses indicated that these specimens represent 183 species of Chalcididae in 10 different genera, with 173 new to Peru and 134 potentially new species. We submitted 268 specimens, representing 167 species, to DNA barcoding. Of these, 141 specimens yielded sequences, 136 of them with a minimum of 300 bp. Sixty specimens were assigned a BIN by the Barcode of Life Database System (BOLD), resulting in 50 BINs. A cluster analysis of 138 individuals that yielded DNA sequences longer than 100 bp revealed 118 MOTUs (molecular operative taxonomic units), all of them highly congruent with the morphological data. Prior to the present study, 37 species in 9 genera of Chalcididae were known from Peru. With our results, this number was increased to 210 species in 13 genera. The present study is the result of a joint effort between the SNSB - Zoologische Staatssammlung München, Germany (ZSM) and the Insect Biodiversity Laboratory of the Universidade Federal do Espírito Santo, Vitória, Brazil (LaBI-UFES), intending to apply an accelerated taxonomic treatment of the Chalcididae of the Panguana reserve using traditional morphological approaches in combination with DNA barcoding. The complete molecular dataset and associated voucher information is publicly available through BOLD. The new species that were discovered as part of the study are being formally described elsewhere as part of taxonomic treatments of Neotropical and world generic revisions at LaBI-UFES.

Redescription of Orthopristis ruber and Orthopristis scapularis (Haemulidae: Perciformes), with a hybridization zone off the Atlantic coast of South America

Zootaxa ◽  
2019 ◽  
Vol 4576 (1) ◽  
pp. 109 ◽  
Author(s):  
ALEXANDRE PIRES MARCENIUK ◽  
RODRIGO ANTUNES CAIRES ◽  
LEONARDO MACHADO ◽  
NAJILA NOLIE CATARINE DANTAS CERQUEIRA ◽  
RAYLA ROBERTA M. DE S. SERRA ◽  
...  
Keyword(s):  
South America ◽  
Nuclear Dna ◽  
Atlantic Coast ◽  
Morphological Characteristics ◽  
Distinct Species ◽  
Valid Species ◽  
Brazilian Coast ◽  
Northern Coast ◽  
Western Atlantic ◽  
Coloration Patterns

The genus Orthopristis includes seven valid species, three from the western Atlantic and five from eastern Pacific, while the available identification guides and taxonomic keys incorrectly recognize Orthopristis ruber as the only valid species found on the Atlantic coast of South America. Efforts to expand the inventory of fish species from the northern coast of Brazil led to the identification of two distinct species of Orthopristis from Atlantic South America, based on the analysis of coloration patterns and meristic data, as well as DNA. In the present study, the limits of Orthopristis ruber are reviewed, while Orthopristis scapularis is recognized as a valid species for the northern and northeastern coasts of South America. Based on intermediate morphological characteristics and nuclear DNA markers, a hybrid zone was identified off the state of Espírito Santo, on the eastern Brazilian coast. Additionally, considerations are made on the diversity and biogeography of the coastal marine and estuarine fishes found on the Brazilian coast. 

scholarly journals Tardigrades of Kristianstads Vattenrike Biosphere Reserve with description of four new species from Sweden

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Edoardo Massa ◽  
Roberto Guidetti ◽  
Michele Cesari ◽  
Lorena Rebecchi ◽  
K. Ingemar Jönsson
Keyword(s):  
New Species ◽  
Laser Scanning ◽  
New Records ◽  
Biosphere Reserve ◽  
Morphological Characters ◽  
Sem Analysis ◽  
Morphological Data ◽  
Integrative Approach ◽  
Confocal Laser ◽  
Biological Value

AbstractKristianstads Vattenrike Biosphere Reserve [KVBR] is a UNESCO designated area of Sweden possessing high biological value. Although several studies on tardigrades inhabiting Sweden have been performed, the KVBR area has been neglected. The current study investigates the tardigrade fauna of five areas of the biosphere reserve and includes 34 samples of different substrates analysed quantitatively and qualitatively. In total, 33 species of tardigrades were found in the samples, including 22 new records for the Skåne region, 15 new records for Sweden, and four species new to science. Mesobiotus emiliae sp. nov., Xerobiotus gretae sp. nov., Itaquascon magnussoni sp. nov., and Thulinius gustavi sp. nov. were described with an integrative approach (when possible) using morphological characters (light, electron scanning, and confocal laser scanning microscopies) and molecular markers (ITS2, 18S, 28S, cox1). A new protocol to increase morphological data was developed recovering mounted specimens within old slides for SEM analysis. Emended diagnoses for the genus Itaquascon and the transfer of Platicrista itaquasconoide to the genus Meplitumen are proposed. This study enriches the knowledge of the tardigrade biodiversity both within the KVBR and in Sweden and contributes to the rapidly increasing number of tardigrade species reported worldwide. The 33 species identified in the KVBR area represents 28% of all water bear species found in Sweden so far. The restricted study areas and limited number of samples collected suggests that the KVBR is very rich of tardigrades.

A new species of Dichelyne (Nematoda, Cucullanidae) parasitizing sciaenid fishes from off the South American Atlantic coast

2009 ◽  
Vol 54 (1) ◽  
Author(s):  
Juan Timi ◽  
Ana Lanfranchi ◽  
Luiz Tavares ◽  
José Luque
Keyword(s):  
New Species ◽  
Atlantic Coast ◽  
Length Distribution ◽  
Distribution Patterns ◽  
Nematode Species ◽  
Valid Species ◽  
South American ◽  
Type Specimens ◽  
Pacific Waters ◽  
A New Species

AbstractA new nematode species Dichelyne (Cucullanellus) sciaenidicola sp. nov. is described based on specimens collected from the Whitemouth croaker Micropogonias furnieri (Desmarest) and the Argentine croaker Umbrina canosai Berg, from coastal waters of Argentina and Brazil. These nematodes were firstly identified as D. (C.) elongatus (Törnquist, 1931), a commonly reported species from M. furnieri in South American Atlantic waters. However, other species of Dichelyne have so far been reported from this host in the same area, namely D. (C.) rodriguesi (Pinto, Fábio et Noronha, 1970), D. (C.) amaruincai (Freitas, Vicente et Ibañez, 1969) and D. (Dichelyne) micropogonii Pereira et Costa, 1996. A careful re-examination of these parasites, as well as of type specimens of all species reported from M. furnieri, revealed that these nematodes represented a new species. The new species is distinguished from most of its congeners by having papillae 5–7 and 9 forming a subventral line close to cloaca, this feature is shared with other 6 species [D. (C.) dichelyneformis (Szidat, 1950), D. (C.) fraseri (Baylis, 1929), D. (C.) abbreviatus (Rudolphi, 1819), D. (C.) adriaticus (Törnquist, 1931), D. (C.) minutus (Rudolphi, 1819) and D. (C.) mariajuliae Alarcos, Timi, Etchegoin et Sardella, 2006)], which are readily distinguished by their body size, spicules length, distribution patterns of other papillae and position of the excretory pore and deirids. Also, D. (C.) elongatus from Umbrina canariensis (Valenciennes) from West Africa is established as a new species Dichelyne (Cucullanellus) yvonnecampanae sp. nov.; D. (C.) amaruincai from Pacific waters is considered as a valid species, D. (D.) micropogonii is regarded as species inquirendae and D. (C.) rodriguesi is identified as Cucullanus sp.

DNA barcoding and phylogenetic relationship of shrimps (Crustacea: Decapoda) of Bangladesh

2021 ◽  
Vol 7 (1) ◽  
pp. 941-946
Author(s):  
Md Sagir Ahmed ◽  
Sumaiya Salam ◽  
Sayeda Sabrina Sarwar Rumana ◽  
Anindita Barua
Keyword(s):  
Dna Barcoding ◽  
Genetic Divergence ◽  
New Records ◽  
Coi Gene ◽  
Identification System ◽  
Taxonomic Rank ◽  
Macrobrachium Nipponense ◽  
Shrimp Species ◽  
The Mean ◽  
Relationship Of

We adopted DNA barcoding technique using a 658-bp fragment of the mitochondrial cytochrome c oxidase I (COI) gene to identify shrimp species collected from the different areas of Bangladesh. A total of 24 sequences were generated belonging to 14 species including four new records- Macrobrachium nipponense, Macrobrachium kistnense, Exopalaemon carinicauda and Alpheus malleator. Genetic distance measured with Kimura 2 parameter showed that genetic divergence increased with higher taxonomic rank. The mean genetic divergence was evaluated and found to be 0.935%, 22.67% and 30.92% within species, genus and family, respectively. In addition to the barcode-based species identification system, phylogenetic relationships were established where individuals belonging to the same species were grouped under the same clade. Maximum likelihood (ML) was preferred as the statistical method and as expected, the phylogenetic tree complemented and ensured the conventional taxonomy. The present study evidently showed that DNA barcoding can be served as an effective tool to discriminate the shrimp species and this will enhance the understanding on evolution and conservation biology. Bioresearch Commu. 7(1): 941-946, 2021 (January)

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