Morphological evidence from immature stages further suggests Lignyodina being close to Tychiina (Coleoptera, Curculionidae, Curculioninae, Tychiini)

The relationship between the subtribes Lignyodina and Tychiina of the Tychiini were tested on the basis of morphological characters from the immature stages. The mature larvae of Lignyodes bischoffi (Blatchley, 1916) and L. enucleator (Panzer, 1798) (Curculionidae, Curculioninae, Tychiini, Lignyodina) and the pupae of L. bischoffi are described in detail for the first time. To be sure about the correct taxonomic identification of the larvae, DNA sequences were obtained and compared for both the larvae and adults of these two species. Molecular data show that the two studied species of Lignyodes Dejean, 1835 strongly differ in mitochondrial COI and 16S rRNA. The immature stages (larva and pupa) of Lignyodes bischoffi and L. enucleator were compared with each other and with those known for other taxa representative of the tribe Tychiini and other tribes of Curculioninae. The larvae of Lignyodes bischoffi and L. enucleator differ in a few but important characters. The larvae and pupae of these two species belonging to Lignyodes s. str. are morphologically very similar to those of species in the other two subgenera of Lignyodes, Neotylopterus Clark, Whitehead & Warner, 1977 and Chionanthobius Pierce, 1912. On the basis of the detailed morphology of larvae and pupae, we agree that Lignyodes belong to Tychiini, sharing more features with species of this tribe than with those of other tribes of Curculioninae. New bionomic data concerning larval and pupal development and adult emergence are reported for both described species and confirm close relationships between the subtribes Lignyodina and Tychiina. On the other hand, strong molecular differences, corroborated by some relevant larval differences, between L. enucleator and L. bischoffi open up the issue whether Palaearctic and Nearctic species presently included in Lignyodes s. str. actually belong to the same subgenus.

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Male identification, generic classification and sexual dimorphism of Micronychus pardus (Kazantsev, 2018) comb. nov. (Coleoptera: Lycidae: Calochrominae)

Zootaxa ◽

10.11646/zootaxa.4657.1.9 ◽

2019 ◽

Vol 4657 (1) ◽

pp. 177-182 ◽

Cited By ~ 2

Author(s):

MICHAL MOTYKA

Keyword(s):

Mitochondrial Dna ◽

Sexual Dimorphism ◽

Species Identification ◽

Dna Sequences ◽

Morphological Characters ◽

Molecular Data ◽

Large Specimen ◽

Molecular Analyses ◽

Almost All ◽

First Time

Almost all net-winged beetles are members of Müllerian complexes and their similarity due to phenotypic coevolution sometimes complicates species identification and generic placement. Therefore, large specimen series, detailed exhaustive examination of morphological characters and molecular data are needed to clarify the taxonomic placement. Using mitochondrial DNA sequences, I investigated the sexual dimorphism and generic placement of the recently described species Calochromus pardus Kazantsev, 2018. I found that the species does not belong in Calochromus Guérin-Méneville, 1833 and all morphological characters and molecular analyses point to its placement in Micronychus Motschulsky, 1861. Therefore, Micronychus pardus (Kazantsev, 2018), comb. nov. is proposed. Additionally, the male is described here for the first time showing the sexual dimorphism in the species. Unlike the females, the males do not superficially resemble members of Xylobanus Waterhouse, 1879 with bright coloured elytral costae and black background, but mimics the sympatrically occurring yellow and black lycids in the genus Cautires Waterhouse, 1879.

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Immature stages of Palearctic Mecinus species (Coleoptera, Curculionidae, Curculioninae): morphological characters diagnostic at genus and species levels

ZooKeys ◽

10.3897/zookeys.939.50612 ◽

2020 ◽

Vol 939 ◽

pp. 87-165 ◽

Cited By ~ 1

Author(s):

Rafał Gosik ◽

Jiří Skuhrovec ◽

Roberto Caldara ◽

Ivo Toševski

Keyword(s):

Phylogenetic Analysis ◽

Morphological Characters ◽

Biological Data ◽

The Other ◽

Taxonomic Identification ◽

Immature Stages ◽

Differential Characters ◽

Species Groups ◽

Thoracic And Abdominal ◽

First Time

The immature stages of ten Mecinus species are described for the first time and those of two other species are redescribed, adding important chaetotaxy characters that were missing from previous descriptions. These species belong to six of the nine assemblages of Mecinus species previously established according to a phylogenetic analysis. All these groupings are confirmed on the basis of several characters of mature larvae and pupae. Moreover, all the species show several characters that are useful for distinguishing them from each other, including cryptic species that previously had few differential characters. Some characters that may be useful for separating Mecinus from other genera in the tribe are suggested. To confirm the taxonomic identification of some larvae, the mtCOII gene was obtained and compared with sequences from identified adult specimens. The most important characters for separating the immature stages of the genera and species groups in Mecinus are the number of palpomeres of the labial palpi (1 or 2), the number of air tubes of the thoracic and abdominal spiracles (unicameral or bicameral), and the number of epipharyngeal setae. The species studied herein were compared with those known from other genera in the tribe Mecinini. Two keys, one to the described larvae and the other to the pupae, are provided. Detailed biological data, several of which are new, on some species are reported.

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Morphological characters of immature stages of Palaearctic species of Cleopomiarus and Miarus and their systematic value in Mecinini (Coleoptera, Curculionidae, Curculioninae)

ZooKeys ◽

10.3897/zookeys.808.28172 ◽

2018 ◽

Vol 808 ◽

pp. 23-92 ◽

Cited By ~ 2

Author(s):

Jiří Skuhrovec ◽

Rafał Gosik ◽

Roberto Caldara ◽

Ivo Toševski ◽

Jacek Łętowski ◽

...

Keyword(s):

Morphological Characters ◽

Taxonomic Identification ◽

Immature Stages ◽

Specific Character ◽

Palaearctic Species ◽

Medium Length ◽

First Time ◽

The Relationship

The relationship between the generaCleopomiarusandMiarusof Mecinini (Curculionidae, Curculioninae) was tested on the basis of morphological characters from the immature stages. The mature larvae of fiveCleopomiarusspecies (C.distinctus(Boheman, 1845),C.graminis(Gyllenhal, 1813),C.longirostris(Gyllenhal, 1838),C.medius(Desbrochers des Loges, 1893), andC.meridionalis(H. Brisout de Barneville, 1863)), threeMiarusspecies (M.abnormisSolari, 1947,M.ajugae(Herbst, 1795), andM.campanulae(Linnaeus, 1767)), and the pupae of fourCleopomiarusspecies (C.distinctus,C.graminis,C.longirostris, andC.medius) and twoMiarusspecies (M.abnormisandM.ajugae) are described in detail for the first time. To confirm the taxonomic identification of some larvae, DNA COI barcode was obtained and compared with those of adults. The immature stages of the species herein studied were compared with those known from other genera in tribe Mecinini. It is suggested thatMiarusandCleopomiarusmay be monophyletic based on several shared distinctive characters. Larvae ofMiarushave a characteristic maxillary mala with six finger-likedmsof two sizes (one or twodmsvery long and the rest of medium length), this feature being apparently unique among weevils. Other genus-specific character states are observed in the pupae, such as the length of setae on the head, rostrum and pronotum, including the number ofrson the rostrum,dson pronotum, and finally the shape of the urogomphi. A key to the described larvae and pupae were respectively presented. New biological and distributional data on some species are reported.

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Mosquitoes of the Anopheles maculipennis group (Diptera: Culicidae) in Romania, with the discovery and formal recognition of a new species based on molecular and morphological evidence

Bulletin of Entomological Research ◽

10.1079/ber2004330 ◽

2004 ◽

Vol 94 (6) ◽

pp. 525-535 ◽

Cited By ~ 63

Author(s):

G. Nicolescu ◽

Y.-M. Linton ◽

A. Vladimirescu ◽

T.M. Howard ◽

R.E. Harbach

Keyword(s):

New Species ◽

Dna Sequences ◽

Coastal Region ◽

Distinct Species ◽

Morphological Characters ◽

Danube River ◽

The Black Sea ◽

Morphological Evidence ◽

Mitochondrial Coi ◽

Anopheles Maculipennis

AbstractMosquitoes of the Anopheles maculipennis group were collected in five districts of Romania (Constanta, Giurgiu, Ilfov, Mehedinti and Suceava) between March 2000 and June 2003. Two hundred and ninety-seven specimens were identified by molecular methods. Nuclear rDNA ITS2 sequences of 178 specimens were compared with GenBank sequences for nine known Palaearctic species of the group, and 119 specimens were identified using an ITS2 PCR–RFLP assay developed during the study. Five genetically distinct species of the group were identified: A. atroparvus van Thiel, A. maculipennis Meigen, A. melanoon Hackett and A. messeae Falleroni and a previously unrecognized species. The new species, herein formally described and named A. daciaesp. n., was collected in the Black Sea coastal region and plains adjacent to the Danube River in southern Romania. Anopheles daciae is most similar to and sympatric with A. messeae. It is contrasted with A. messeae and characterized on the basis of unique nuclear ITS2 and mitochondrial COI DNA sequences and morphological characters of the eggs. The larval, pupal and adults stages of the two species were also compared, but no reliable characters were found to distinguish them. It seems likely that A. daciae is more widespread in eastern Europe and the Balkan States, and could be responsible for malaria transmission in these regions that is currently attributed to A. messeae. Anopheles melanoon is reported from Romania for the first time.

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Are Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara (Acari: Tetranychidae) one species? Morphological and molecular evidences for synonymy between these two spider mite species and a note on invasiveness of T. macfarlanei on okra and eggplant in India

Systematic and Applied Acarology ◽

10.11158/saa.22.4.3 ◽

2017 ◽

Vol 22 (4) ◽

pp. 467

Author(s):

Mahran Zeity ◽

Nagappa Srinivas ◽

Chinnamade Channegowde Gowda

Keyword(s):

Spider Mite ◽

Morphological Characters ◽

Molecular Data ◽

Mite Species ◽

Coi Gene ◽

High Similarity ◽

Mitochondrial Coi ◽

Mitochondrial Coi Gene ◽

High Degree ◽

Degree Of Similarity

Study of morphological characters of Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara revealed high similarity by comparing all the important characters in addition to the characters pointed out by Ehara to separate those two species. Molecular phylogeny of seven Indian populations of T. macfarlanei and one population of T. malaysiensis from Philippines along with few distantly related species of Tetranychus was attempted. High degree of similarity between these two species at mitochondrial COI gene (96%) as well as ITS2 (rDNA) (96–99%) region was evident. Based on both morphological features and molecular data, T. malaysiensis is proposed as a junior synonym of T. macfarlanei based on ICZN’s law of priority. Also more female characters are prompted in this study to distinctly discriminate T. macfarlanei from its most resembling species, Tetranychus ludeni Zacher. Tetranychus macfarlanei has emerged as a pest of several cultivated crop plants in India.

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Sinalliaria, a new genus of Brassicaceae from eastern China, based on morphological and molecular data

Phytotaxa ◽

10.11646/phytotaxa.186.4.2 ◽

2014 ◽

Vol 186 (4) ◽

pp. 188 ◽

Cited By ~ 6

Author(s):

Ying-Ying Zhou ◽

HONG-WEI ZHANG ◽

JIANG-QIN HU ◽

Xiao-Feng Jin

Keyword(s):

Dna Sequences ◽

New Genus ◽

Eastern China ◽

Distinct Group ◽

Morphological Characters ◽

Molecular Data ◽

New Combinations ◽

The Family ◽

Morphological And Molecular Data ◽

Seed Testa

Sinalliaria is described here as a new genus of the family Brassicaceae from eastern China, based on the morphological characters and molecular sequences. Sinalliaria differs from the related genus Orychophragmus in having basal leaves petiolate, simple or rarely with 1‒3 lateral lobes (not pinnatisect); cauline leaves petiolate, cordate at base (not sessile, auriculate or amplexicaul at base); petals obovate to narrowly obovate, claw inconspicuous (not broadly obovate, with a claw as along as sepal); siliques truncate (not long-beaked) at apex. The microscopic characters of seed testa also show significant differences between Sinalliaria and Orychophragmus. Phylogenetic evidence from DNA sequences of nuclear ribosomal ITS and plastid region trnL-trnF indicates that Sinalliaria is a distinct group related to Orychophragmus and Raphanus, but these three genera do not form a clade. The new genus Sinalliaria is endemic to eastern China and has only one species and one variety. The new combinations, S. limprichtiana (Pax) X. F. Jin, Y. Y. Zhou & H. W. Zhang and S. limprichtiana var. grandifolia (Z. X. An) X. F. Jin, Y. Y. Zhou & H. W. Zhang are proposed here.

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Entomophilic nematodes, Diploscapter coronatus and Oscheius tipulae from Afghanistan

Zootaxa ◽

10.11646/zootaxa.4926.3.5 ◽

2021 ◽

Vol 4926 (3) ◽

pp. 401-416

Author(s):

MOHAMMAD HUSSAIN FALAHZADAH ◽

EBRAHIM SHOKOOHI ◽

GHOLAM HOSSEIN MORAVEJ ◽

PHATU WILLIAM MASHELA ◽

ABDUL KHALID MADADI ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Genetic Distance ◽

Galleria Mellonella ◽

Morphological Characters ◽

Molecular Data ◽

Soil Samples ◽

The Body ◽

Morphometric Data ◽

Molecular Approaches ◽

The Relationship

Several soil samples from different habitats in Badakhshan province of Afghanistan were collected to isolate and characterize bacteria feeding nematodes. The Galleria mellonella-baiting method was used for the isolation of the Afghan insect-associated nematodes. The nematodes were studied using morphological and morphometric data. The Oscheius specimen was characterized by a longer body (630–820 µm) and shorter pharynx (125–145 µm), whereas other morphological characters were not unusual. The Diploscapter specimen had an annulated cuticle, with lip region width 1.5 times shorter than the stoma, and had separated pharyngeal corpus from the isthmus and vulva located in the middle of the body. The molecular data were derived using three loci; 18S, 28S (D2/D3 segment), and ITS rRNA region, which were utilized to measure the genetic distance. The phylogenetic analysis was conducted to reconstruct the relationship tree. Both morphological and molecular approaches confirmed the identity of nematode isolates as Oscheius tipulae and Diploscapter coronatus. This is the first report of insect-associated nematodes from the soil of Afghanistan. Both species were capable of infecting and killing G. mellonella larvae in less than 96 h.

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Three new species of the genus Coddingtonia from Asia (Araneae, Theridiosomatidae)

ZooKeys ◽

10.3897/zookeys.886.35492 ◽

2019 ◽

Vol 886 ◽

pp. 113-126

Author(s):

Chengcheng Feng ◽

Yucheng Lin

Keyword(s):

New Species ◽

Dna Sequences ◽

Morphological Characters ◽

Molecular Data ◽

Current Paper ◽

Distribution Map ◽

Three New Species

The current paper expands knowledge of the genus Coddingtonia Miller, Griswold & Yin, 2009. Based on morphological characters and molecular data, three species are documented as new to science: C. erhuan Feng & Lin, sp. nov. (♀) from China, C. lizu Feng & Lin, sp. nov. (♀) from China, and C. huifengi Feng & Lin, sp. nov. (♂♀) from Indonesia. The type of C. euryopoides Miller, Griswold & Yin, 2009 is also reexamined. DNA sequences (COI), detailed illustrations of habitus, male palp and epigyne are provided for these four species, as well as a key and a distribution map for Coddingtonia species.

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Amanita subjunquillea and its ectomycorrhizal association, reported as new for Pakistan

Mycotaxon ◽

10.5248/134.413 ◽

2019 ◽

Vol 134 (3) ◽

pp. 413-423

Author(s):

Muhammad Ishaq ◽

Arooj Naseer ◽

Munazza Kiran ◽

Muhammad Fiaz ◽

Abdul Nasir Khalid

Keyword(s):

Its Region ◽

Morphological Characters ◽

Molecular Data ◽

Sequence Analyses ◽

Dark Orange ◽

Ectomycorrhizal Roots ◽

Morphological And Molecular Data ◽

Quercus Floribunda ◽

First Time ◽

Ectomycorrhizal Association

Amanita subjunquillea and its ectomycorrhizal association are reported for the first time from moist temperate Himalayan forests of Pakistan. The sample was studied based on morphological characters and nucleotide sequence analyses of the ITS region generated from basidiomata and ectomycorrhizal roots of Quercus floribunda. Our collection differs from the type in its dark orange pileus disc and pale yellow margins. Remaining morphological and molecular data are consistent with previously reported specimens. This represents the first report of A. subjunquillea from Pakistan.

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An integrated approach for synonymization of Rotylenchus rhomboides with R. goodeyi (Nematoda: Hoplolaimidae) reveals high intraspecific mitogenomic variation

Phytopathology ◽

10.1094/phyto-08-21-0363-r ◽

2021 ◽

Author(s):

Phougeishangbam Rolish Singh ◽

Bart van de Vossenberg ◽

Katarzynar Rybarczyk-Mydłowska3 ◽

Magdalena Kowalewska-Groszkowska ◽

Wim Bert ◽

...

Keyword(s):

Dna Sequences ◽

Integrated Approach ◽

Morphological Characters ◽

Molecular Data ◽

Cox1 Gene ◽

Protein Coding ◽

Genome Shotgun Sequence ◽

Important Character ◽

Rna Genes ◽

Level Identification

Rotylenchus is a widely-distributed economically important plant-parasitic nematode group whose species-level identification relies largely on limited morphological characters including character-based tabular keys and molecular data of ribosomal and mitochondrial genes. In this study, a combined morphological and molecular analysis of three populations of R. goodeyi from Belgium, Poland and the Netherlands revealed important character variations of this species leading to synonymisation of R. rhomboides with R. goodeyi, and a high nucleotide variation within cox1 gene sequences in these populations. Additional Illumina sequencing of DNA from individuals of the Dutch population revealed two variants of mitogenomes each of approximately 23 Kb in size, differing by about 9% and containing eleven protein coding genes, two ribosomal RNA genes and up to 29 transfer RNA genes. In addition to the first representative whole genome shotgun sequence datasets of the genus Rotylenchus, this study also provides the full length mitogenome and the ribosomal DNA sequences of R. goodeyi.

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