Morphology and description of infraciliary bands pattern in four Metadinium Awerinzew & Mutafowa, 1914 species (Ciliophora, Entodiniomorphida, Ophryoscolecidae) with taxonomic notes on the genus

Zootaxa ◽  
2018 ◽  
Vol 4500 (4) ◽  
pp. 574 ◽  
Author(s):  
FRANCIANE CEDROLA ◽  
MARIANA FONSECA ROSSI ◽  
ISABEL MARTINELE ◽  
MARTA D’AGOSTO ◽  
ROBERTO JÚNIO PEDROSO DIAS
Keyword(s):  
Host Species ◽  
Morphological Similarity ◽  
Type Pattern ◽  
Anterior Body ◽  
Contractile Vacuoles ◽  
Geographical Locations

The genus Metadinium includes ophryoscolecid ciliates, with two retractile ciliary zones in the anterior body portion, two broad skeletal plates, a lobed macronucleus and two contractile vacuoles. Species belonging to this genus were recorded in several geographical locations and in different host species. However, they were mostly described based on a single ciliatological technique, and until now, there is only one description of the infraciliary pattern in Metadinium ciliates (Metadinium medium). The present study performs the morphological and infraciliature characterization of four species belonging to Metadinium: M. esalqum, M. minorum, M. rotundatum and M. ypsilon; and presents taxonomic notes on the genus. The oral infraciliature in these four Metadinium species corresponds to the Diplodinium-type, pattern shared with genera Diplodinium, Diploplastron, Eudiplodinium, Eremoplastron and Polyplastron. A brief review conducted highlights the difficulty of delimiting Metadinium species due to the morphological similarity among them. 

scholarly journals Characterization of the Intergenic Spacer rDNAs of Two Pig Nodule Worms,Oesophagostomum dentatumandO. quadrispinulatum

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Rui-Qing Lin ◽  
Li Shu ◽  
Guang-Hui Zhao ◽  
Tian Cheng ◽  
Shang-Shu Zou ◽  
...  
Keyword(s):  
Mainland China ◽  
Intergenic Spacer ◽  
Oesophagostomum Dentatum ◽  
Rdna Sequences ◽  
Length Polymorphisms ◽  
Different Types ◽  
Different Origins ◽  
Geographical Locations ◽  
Independent Species

The characteristics of the intergenic spacer rDNAs (IGS rDNAs) ofOesophagostomum dentatumandO. quadrispinulatumisolated from pigs in different geographical locations in Mainland China were determined, and the phylogenetic relationships of the two species were reconstructed using the IGS rDNA sequences. The organization of the IGS rDNA sequences was similar to their organization in other eukaryotes. The 28S-18S IGS rDNA sequences of bothO. dentatumandO. quadrispinulatumwere found to have variable lengths, that is, 759–762 bp and 937–1128 bp, respectively. All of the sequences contained direct repeats and inverted repeats. The length polymorphisms were related to the different numbers and organization of repetitive elements. Different types and numbers of repeats were found between the two pig nodule species, and two IGS structures were found withinO. quadrispinulatum. Phylogenetic analysis showed that allO. dentatumisolates were clustered into one clade, butO. quadrispinulatumisolates from different origins were grouped into two distinct clusters. These results suggested independent species and the existence of genotypes or subspecies within pig nodule worms. Different types and numbers of repeats and IGS rDNA structures could serve as potential markers for differentiating these two species of pig nodule worms.

Characterization of Fasciola samples from different host species and geographical localities in Spain by sequences of internal transcribed spacers of rDNA

2007 ◽  
Vol 101 (5) ◽  
pp. 1245-1250 ◽  
Author(s):  
S. Alasaad ◽  
C. Q. Huang ◽  
Q. Y. Li ◽  
J. E. Granados ◽  
C. García-Romero ◽  
...  
Keyword(s):  
Host Species ◽  
Internal Transcribed Spacers

scholarly journals Comparative analysis and characterization of the gut microbiota of four farmed snakes from southern China

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6658 ◽  
Author(s):  
Bing Zhang ◽  
Jing Ren ◽  
Daode Yang ◽  
Shuoran Liu ◽  
Xinguo Gong
Keyword(s):  
Gut Microbiota ◽  
Host Species ◽  
High Throughput Sequencing ◽  
Southern China ◽  
Alpha Diversity ◽  
Operational Taxonomic Units ◽  
Close Relationship ◽  
Elaphe Carinata ◽  
Deinagkistrodon Acutus

Background The gut microbiota plays an important role in host immunity and metabolic homeostasis. Although analyses of gut microbiotas have been used to assess host health and foster disease prevention and treatment, no comparative comprehensive study, assessing gut microbiotas among several species of farmed snake, is yet available. In this study, we characterized and compared the gut microbiotas of four species of farmed snakes (Naja atra, Ptyas mucosa, Elaphe carinata, and Deinagkistrodon acutus) using high-throughput sequencing of the 16S rDNA gene in southern China and tested whether there was a relationship between gut microbiotal composition and host species. Results A total of 629 operational taxonomic units across 22 samples were detected. The five most abundant phyla were Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, and Actinobacteria, while the five most abundant genera were Bacteroides, Cetobacterium, Clostridium, Plesiomonas, and Paeniclostridium. This was the first report of the dominance of Fusobacteria and Cetobacterium in the snake gut. Our phylogenetic analysis recovered a relatively close relationship between Fusobacteria and Bacteroidetes. Alpha diversity analysis indicated that species richness and diversity were highest in the gut microbiota of D. acutus and lowest in that of E. carinata. Significant differences in alpha diversity were detected among the four farmed snake species. The gut microbiotas of conspecifics were more similar to each other than to those of heterospecifics. Conclusion This study provides the first comparative study of gut microbiotas among several species of farmed snakes, and provides valuable data for the management of farmed snakes. In farmed snakes, host species affected the species composition and diversity of the gut microbiota.

scholarly journals Characterization of the β-lactamase specified by the resistance factor R-1818 in Escherichia coli K12 and other Gram-negative bacteria

1971 ◽  
Vol 123 (4) ◽  
pp. 501-505 ◽  
Author(s):  
J. W. Dale
Keyword(s):  
Escherichia Coli ◽  
Host Species ◽  
Host Bacterium ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
R Factor ◽  
Relationship Of ◽  
The Relationship

1. The amino acid composition of the β-lactamase from E. coli (R-1818) was determined. 2. The R-1818 β-lactamase is inhibited by formaldehyde, hydroxylamine, sodium azide, iodoacetamide, iodine and sodium chloride. 3. The Km values for benzylpenicillin, ampicillin and oxacillin have been determined by using the R-factor enzyme from different host species. The same values were obtained, irrespective of the host bacterium. 4. The molecular weight of the enzyme was found to be 44600, and was the same for all host species. 5. The relationship of R-1818 and R-GN238 β-lactamases is discussed.

Unexpected conservation and global transmission of agrobacterial virulence plasmids

Science ◽  
2020 ◽  
Vol 368 (6495) ◽  
pp. eaba5256 ◽  
Author(s):  
Alexandra J. Weisberg ◽  
Edward W. Davis ◽  
Javier Tabima ◽  
Michael S. Belcher ◽  
Marilyn Miller ◽  
...  
Keyword(s):  
Host Species ◽  
Evolutionary History ◽  
Virulence Plasmids ◽  
Ri Plasmid ◽  
Accelerated Evolution ◽  
Transfer Genes ◽  
Global Spread

The accelerated evolution and spread of pathogens are threats to host species. Agrobacteria require an oncogenic Ti or Ri plasmid to transfer genes into plants and cause disease. We developed a strategy to characterize virulence plasmids and applied it to analyze hundreds of strains collected between 1927 and 2017, on six continents and from more than 50 host species. In consideration of prior evidence for prolific recombination, it was surprising that oncogenic plasmids are descended from a few conserved lineages. Characterization of a hierarchy of features that promote or constrain plasticity allowed inference of the evolutionary history across the plasmid lineages. We uncovered epidemiological patterns that highlight the importance of plasmid transmission in pathogen diversification as well as in long-term persistence and the global spread of disease.

scholarly journals Molecular Characterization of Glycopeptide-Resistant Enterococcus faecium Isolates from Portuguese Hospitals

2005 ◽  
Vol 49 (7) ◽  
pp. 3073-3079 ◽  
Author(s):  
Carla Novais ◽  
João C. Sousa ◽  
Teresa M. Coque ◽  
Luísa V. Peixe
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant ◽  
Geographical Locations

ABSTRACT Fifty-one pulsed-field gel electrophoresis types and 17 Tn1546 variants were identified among 101 Enterococcus faecium isolates recovered in three distant Portuguese hospitals. Intra- and interhospital dissemination of specific strains and Tn1546 types was detected, which might largely contribute to the endemicity of vancomycin-resistant E. faecium in Portuguese hospitals, as happened previously in other geographical locations.

scholarly journals Identification, cloning, and characterization of PWL2, a gene for host species specificity in the rice blast fungus.

1995 ◽  
Vol 7 (8) ◽  
pp. 1221-1233 ◽  
Author(s):  
J A Sweigard ◽  
A M Carroll ◽  
S Kang ◽  
L Farrall ◽  
F G Chumley ◽  
...  
Keyword(s):  
Host Species ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Species Specificity ◽  
Blast Fungus

scholarly journals Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland

2017 ◽  
Vol 66 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Beata Zimowska ◽  
Ewa Dorota Zalewska ◽  
Ewa Dorota Król ◽  
Agnieszka Furmańczyk
Keyword(s):  
Culture Media ◽  
Culture Collection ◽  
Morphological Similarity ◽  
Reference Isolate ◽  
Angelica Archangelica ◽  
Its Regions ◽  
Standard Culture ◽  
First Time ◽  
Morphological And Molecular Characterization

The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of the fungus to the species.

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