Taxonomic revision of Algero-Tunisian Pleurodeles (Caudata: Salamandridae) using molecular and morphological data. Revalidation of the taxon Pleurodeles nebulosus (Guichenot, 1850)

Zootaxa ◽  
2004 ◽  
Vol 488 (1) ◽  
pp. 1 ◽  
Author(s):  
SALVADOR CARRANZA ◽  
EDWARD WADE
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome B ◽  
Morphometric Analysis ◽  
Dna Sequences ◽  
Taxonomic Status ◽  
Taxonomic Revision ◽  
Morphological Data ◽  
Morphological Evidence ◽  
12S Rrna

The taxonomic status of Algero-Tunisian Pleurodeles was reanalysed in the light of new molecular and morphological evidence. Mitochondrial DNA sequences (396 bp of the cytochrome b and 369 of the 12S rRNA) and the results of the morphometric analysis, indicate that Algero-Tunisian P. poireti consists of two genetically and morphologically distinct forms. One restricted to the Edough Peninsula, and another one covering all the rest of its distribution in Algeria and Tunisia. The name P. poireti (Gervais, 1835) is restricted to the population of the Edough Peninsula, while P. nebulous (Guichenot, 1850) correctly applies to all other populations in the distribution. P. poireti originated approximately 4.2 Myr ago, probably as a result of the Edough Peninsula being a Pliocene fossil island, allowing both forms of Algero-Tunisian Pleurodeles to diverge both genetically and morphologically.

A new subspecies of Short-toed Snake-eagle from Wallacea determined from morphological and DNA comparison

Zootaxa ◽  
2017 ◽  
Vol 4358 (2) ◽  
pp. 365
Author(s):  
NATHANIEL S.R. NG ◽  
LES CHRISTIDIS ◽  
JERRY OLSEN ◽  
JANETTE NORMAN ◽  
FRANK E. RHEINDT
Keyword(s):  
Cytochrome B ◽  
Dna Sequences ◽  
Conservation Status ◽  
Taxonomic Status ◽  
Morphological Data ◽  
Migrant Population ◽  
Pleistocene Glaciations ◽  
New Subspecies ◽  
The Past ◽  
Mitochondrial Cytochrome B

The taxonomic status of the geographically isolated population of Short-toed Snake-eagle Circaetus gallicus on the Lesser Sundas (=Nusa Tenggara) has been controversial. In the past they have been regarded as either a migrant population or a recently arrived resident population. Here we obtained DNA sequences from the mitochondrial cytochrome-b gene and combined genetic data with assessments of published and novel morphological data to clarify the taxonomic status of the Lesser Sundas population of C. gallicus. The cytochrome-b distance between the Lesser Sundas and Palearctic populations of C. gallicus is consistent with subspecific differentiation, indicating a possible divergence during one of the most recent Pleistocene glaciations. Although some of the morphological distinctions show overlap, the new subspecies can generally be diagnosed from nominate C. gallicus gallicus by being smaller and paler, and exhibiting less spotting and barring. Unlike nominate populations from mainland Eurasia, the new subspecies seems to lack pronounced sexual dimorphism. We here describe this new subspecies to science and discuss its biology and conservation status. 

Study on the origin and taxonomic status of yak (Poephagus) using cytochrome b gene of mitochondrial DNA

2007 ◽  
Vol 1 (3) ◽  
pp. 329-333 ◽  
Author(s):  
Qifa Li ◽  
Yinxia Li ◽  
Xingbo Zhao ◽  
Zhenshan Liu ◽  
Yefen Xu ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome B ◽  
Taxonomic Status ◽  
Cytochrome B Gene

Phylogeny of Snub-Nosed Monkeys Inferred from Mitochondrial DNA, Cytochrome B, and 12S rRNA Sequences

2004 ◽  
Vol 25 (4) ◽  
pp. 861-873 ◽  
Author(s):  
Ming Li ◽  
Fuwen Wei ◽  
Chengming Huang ◽  
Ruliang Pan ◽  
Johannes de Ruiter
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome B ◽  
12S Rrna ◽  
Rrna Sequences

An enigma no more: an integrated taxonomic revision of Aenigmetopia Malloch reveals novel phylogenetic placement and four new species (Diptera : Sarcophagidae : Miltogramminae)

2020 ◽  
Author(s):  
Nikolas P. Johnston ◽  
James F. Wallman ◽  
Krzysztof Szpila ◽  
Thomas Pape
Keyword(s):  
New Species ◽  
Taxonomic Revision ◽  
Single Species ◽  
Species Level ◽  
Morphological Data ◽  
Morphological Evidence ◽  
Phylogenetic Placement ◽  
Molecular Phylogenetic ◽  
First Time ◽  
Flesh Flies

Aenigmetopia Malloch is the only endemic genus of miltogrammine flesh flies (Diptera:Sarcophagidae) in Australia and, until now it has been known from a single species, A. fergusoni Malloch. This study constitutes the first comprehensive taxonomic revision of Aenigmetopia. Four new species, Aenigmetopia amissa, sp. nov., A. corona, sp. nov., A. kryptos, sp. nov. and A. pagoni, sp. nov., are described through the integration of molecular and morphological data and characters for genus- and species-level diagnoses are given. Aenigmetopia is included in a molecular phylogenetic analysis for the first time and the genus emerges as the sister taxon to Metopia Meigen, in agreement with morphological evidence.

The mitochondrial DNA haplotypes of snails of the estuarine hydrobiid genus Tatea cross species and biogeographic boundaries

2009 ◽  
Vol 60 (8) ◽  
pp. 861 ◽  
Author(s):  
D. J. Colgan ◽  
P. da Costa
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequences ◽  
Marine Species ◽  
Habitat Diversity ◽  
12S Rrna ◽  
Genetic Structuring ◽  
Dna Variation ◽  
Eastern Australia ◽  
Mitochondrial 12S Rrna ◽  
Marine Littoral

Investigations of estuarine taxa can provide a perspective on phylogeography that complements studies of marine littoral organisms. For example, reductions in gene flow between populations and increased genetic structuring would be expected in estuarine species. The substantial amount of information about marine species and the habitat diversity along long latitudinal spans makes south-eastern Australia an excellent potential location for comparing marine and estuarine taxa. To investigate this potential, we studied the phylogeography of the two species in the estuarine gastropod genus Tatea. These have extensive and broadly overlapping distributions that encompass known marine phylogeographic boundaries. Against expectation, both Tatea species showed a remarkable lack of geographic and inter-specific variability in mitochondrial 12S rRNA (107 specimens) and cytochrome c oxidase subunit I (39) DNA sequences. No major phylogeographic discontinuities were revealed in either species and there was minimal haplotype divergence between them for either 12S rRNA or COI. The patterns of mitochondrial DNA variation discovered in Tatea may be due to a recent selective sweep or range expansion from a population in which there was little variability. Both possibilities are complicated by having to explain the similarity of the patterns in the two species.

Description of a new genus and two new species of killifish (Cyprinodontiformes: Nothobranchiidae) from West Africa, with a discussion of the taxonomic status of Aphyosemion maeseni Poll, 1941

Zootaxa ◽  
2009 ◽  
Vol 2294 (1) ◽  
pp. 1-22 ◽  
Author(s):  
RAINER SONNENBERG ◽  
ECKHARD BUSCH
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
New Genus ◽  
Nuclear Dna ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Color Pattern ◽  
Monophyletic Group ◽  
Morphological Data ◽  
Adult Males

The phylogeny of the West African genus Archiaphyosemion was studied with mitochondrial and nuclear DNA sequences. The results of the combined dataset presented here did not support a monophyletic group. After the exclusion of the type species of the genus, A. guineense, the remaining species form a well-supported monophyletic group. Based on these molecular results and supported by morphological data, we suggest a new name for this group, Nimbapanchax, new genus. Additionally, based on a recent collection in Guinea, two new Nimbapanchax species were described. The taxon Nimbapanchax leucopterygius, new species, is described for a nothobranchiid fish formerly misidentified as Archiaphyosemion maeseni (Poll, 1941). Nimbapanchax melanopterygius, new species, is described from the Mount Nimba region in southeastern Guinea. Both new Nimbapanchax species are clearly distinguished from their congeners by the coloration pattern of adult males. The results of the DNA data support the assumption based on color pattern and morphological characters that the new described species are sister taxa. The type of Aphyosemion maeseni Poll, 1941 was reexamined and transferred to the genus Epiplatys, a decision based on diagnostic morphological characters.

Copro-PCR based detection of bovine schistosome infection in India

2015 ◽  
Vol 90 (1) ◽  
pp. 102-107 ◽  
Author(s):  
B. Lakshmanan ◽  
K. Devada ◽  
S. Joseph ◽  
T.V. Aravindakshan ◽  
L. Sabu
Keyword(s):  
Mitochondrial Dna ◽  
Ribosomal Rna ◽  
Dna Sequences ◽  
Rrna Genes ◽  
12S Rrna ◽  
Gene Sequences ◽  
Schistosome Infection ◽  
Faecal Samples ◽  
Polymerase Chain ◽  
Trematode Infections

AbstractSchistosomosis and amphistomosis are the two economically important and widely prevalent snail-borne trematode infections in grazing cattle of southern India. Acute infections are symptomatically similar and difficult to detect by routine microscopy for eggs. The present study was directed towards the development of a copro-polymerase chain reaction (copro-PCR) for detection of bovine schistosome species, using custom-designed primers targeting 18S and 28S ribosomal RNA as well as mitochondrial DNA. The study demonstrated the enhanced diagnostic specificity of mitochondrial DNA markers over ribosomal RNA genes as genus-specific probes to detect schistosomes. We developed a sensitive PCR assay using primers designed from mitochondrial DNA sequences targeting the partialrrnl(16S rRNA), tCys (transfer RNA for cysteine) and partialrrnS(12S rRNA) genes ofSchistosoma spindaleto specifically detect schistosome infection from faecal samples of naturally infected bovines. The salient findings of the work also throw light on to the high similarity of the ribosomal RNA gene sequences of schistosomes with those ofGastrothylax crumeniferandFischoederius elongatus,the most prevalent pouched amphistomes of the region. Further investigation has to be directed towards unravelling the complete gene sequences of 18S and 28S ribosomal RNA as well as mitochondrial DNA sequences of amphistome isolates from India.

The cosmopolitan spider Cryptachaea blattea (Urquhart 1886) (Araneae: Theridiidae): Redescription, including COI sequence, and new synonymy

Zootaxa ◽  
2009 ◽  
Vol 2133 (1) ◽  
pp. 55-63 ◽  
Author(s):  
COR J. VINK ◽  
NADINE DUPÉRRÉ ◽  
PIERRE PAQUIN ◽  
BRIAN M. FITZGERALD ◽  
PHIL J. SIRVID
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Junior Synonym ◽  
New Combination ◽  
Morphological Evidence ◽  
Cosmopolitan Species ◽  
New Synonymy

Cryptachaea blattea (Urquhart 1886) new combination, a cosmopolitan species of the spider family Theridiidae, is redescribed, including notes on its distribution and DNA sequences from the mitochondrial gene cytochrome c oxidase subunit 1. Based on morphological evidence and mitochondrial DNA sequences, Cryptachaea acoreensis Berland 1932 is a junior synonym of Cryptachaea blattea.

Phylogeographic structure within Phascogale (Marsupialia : Dasyuridae) based on partial cytochrome b sequence

2001 ◽  
Vol 49 (4) ◽  
pp. 369 ◽  
Author(s):  
P. B. S. Spencer ◽  
S. G. Rhind ◽  
M. D. B. Eldridge
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Differentiation ◽  
Cytochrome B ◽  
Sequence Divergence ◽  
Taxonomic Status ◽  
Phylogeographic Structure ◽  
Northern Australia ◽  
Allopatric Populations ◽  
Taxonomic Research

The brush-tailed phascogale (Phascogale tapoatafa) is considered locally rare and vulnerable, despite being found in all mainland states of Australia. It is rarely detected in faunal surveys and the two most immediate conservation requirements are a determination of its current range and clarification of its taxonomic status. Measures of genetic differentiation amongst Phascogale tapoatafa populations in eastern, western and northern Australia were estimated using a partial (348 bp) sequence of mitochondrial DNA (cytochrome b gene). Observed sequence divergence within P. tapoatafa was substantial, with an average of 13% separating the allopatric populations in south-eastern, south-western and northern Australia. In comparison, an average of 16% sequence divergence separated the two currently recognised Phascogale species (P. tapoatafa andP. calura). Thus, Phascogale comprises four highly divergent lineages, suggesting that the genus is more diverse than previously thought. These data indicate that further taxonomic research is warranted.

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