Rapid Biological Synthesis of Silver Nanoparticles and It's In Vitro Anti-Bacterial and Larvicidal Activities
The current investigation explains the Acacia nilotica (A. nilotica) leaf extract capped silver nanoparticle (AgNPs) synthesis. The instant formation of AgNPs was confirmed by altering the color golden yellow to dark brown and the characteristic SPR band determines by the UV-visible spectrometer. The structural specification was assessed using Fourier transform infrared spectrometry (FTIR), X-ray diffraction crystallography (XRD), High-resolution transmission electron microscopy, Atomic force microscopy (AFM), energy dispersive electron microscopy, zeta potential, dynamic light scattering. The particle size ranged 20–80 nm, the average crystalline size 35.26 nm were determined by HR-TEM and XRD. Further in vitro antimicrobial activity was tested against different bacteria by combining antibiotic with the AgNPs. Among all the tested bacteria, the Staphylococcus aureus (S. aureus) showed the highest (16.2 ± 0.6 mm) zone of inhibition. The Scanning electron microscope (SEM) analysis clearly showed the structural damage of S. aureus and Escherichia coli (E. coli) by increasing AgNPs concentration at 4 hr treatment. In addition, the larvicidal assay was performed against Culex quinquefasciatus (C. quinquefasciatus) and Aedes aegypti (A. aegypti). The higher efficiency of AgNPs was absorbed in A. aegypti (LC50 = 279 22 ppm) and C. quinque- fasciatus (LC50 = 168.98 ppm). The results proved that the An-AgNPs act as a multi-faceted effect on bacterial and controlling vectors.