A Zeolite Nanoparticle-Modified Anionic Surface for Aptasensing Lipocalin-2 in Ulcerative Colitis by Dual-Electrodes

2021 ◽  
Vol 17 (12) ◽  
pp. 2495-2504
Author(s):  
Xiangchen Li ◽  
Subash C. B. Gopinath ◽  
Xin Peng ◽  
Jiudi Lv
Keyword(s):  
Ulcerative Colitis ◽  
Sodium Dodecyl ◽  
Spherical Shape ◽  
Lipocalin 2 ◽  
Linear Regression Equation ◽  
Specific Detection ◽  
Sodium Dodecyl Benzenesulfonate ◽  
Lower Detection ◽  
Interdigitated Microelectrode ◽  
Metalloproteinase 9

An aptasensor was developed on an interdigitated microelectrode (IDME) by current-volt sensing for the diagnosis of ulcerative colitis by detecting the biomarker lipocalin-2. Higher immobilization of the anti-lipocalin-2 aptamer as a probe was achieved by using sodium dodecyl benzenesulfonate-aided zeolite particles. FESEM and FETEM observations revealed that the size of the zeolite particles was <200 nm, and they displayed a uniform distribution and spherical shape. XPS analysis attested the occurrence of Si, Al, and O groups on the zeolite particles. Zeolite particles were immobilized on IDME by a (3-aminopropyl)-trimethoxysilane amine linker, and then, the aptamer as the probe was tethered on the zeolite particles through a biotin-streptavidin strategy assisted by a bifunctional aldehyde linker. Due to the high occupancy of the aptamer and the efficient electric transfer from zeolite particles, higher changes in current can be observed upon interaction of the aptamer with lipocalin-2. The lower detection of lipocalin-2 was noted as 10 pg/mL, with a linear range from 10 pg/mL to 1 μg/mL and a linear regression equation of y=8E−07x+8E−08; R2 = 0.991. Control experiments with complementary aptamer and matrix metalloproteinase-9 indicate the specific detection of lipocalin-2. Furthermore, spiking lipocalin-2 in human serum does not interfere with the identification.

scholarly journals Predicting disease course in ulcerative colitis using stool proteins identified through an aptamer-based screen

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sanam Soomro ◽  
Suresh Venkateswaran ◽  
Kamala Vanarsa ◽  
Marwa Kharboutli ◽  
Malavika Nidhi ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Fecal Calprotectin ◽  
Disease Monitoring ◽  
Disease Course ◽  
Lipocalin 2 ◽  
Time Points ◽  
Inflammatory Bowel ◽  
Stool Samples

AbstractIn the search for improved stool biomarkers for inflammatory bowel disease (IBD), an aptamer-based screen of 1129 stool proteins was conducted using stool samples from an IBD cohort. Here we report that of the 20 proteins subsequently validated by ELISA, stool Ferritin, Fibrinogen, Haptoglobin, Hemoglobin, Lipocalin-2, MMP-12, MMP-9, Myeloperoxidase, PGRP-S, Properdin, Resistin, Serpin A4, and TIMP-1 are significantly elevated in both ulcerative colitis (UC) and Crohn’s disease (CD) compared to controls. When tested in a longitudinal cohort of 50 UC patients at 4 time-points, fecal Fibrinogen, MMP-8, PGRP-S, and TIMP-2 show the strongest positive correlation with concurrent PUCAI and PGA scores and are superior to fecal calprotectin. Unlike fecal calprotectin, baseline stool Fibrinogen, MMP-12, PGRP-S, TIMP-1, and TIMP-2 can predict clinical remission at Week-4. Here we show that stool proteins identified using the comprehensive aptamer-based screen are superior to fecal calprotectin alone in disease monitoring and prediction in IBD.

Interaction of Dendrimer-Based Polymer with Sodium Dodecyl Benzenesulfonate: Characterization and Effect on Properties of Composites

2016 ◽  
Vol 30 (11) ◽  
pp. 9362-9371 ◽  
Author(s):  
Nanjun Lai ◽  
Yan Zhang ◽  
Qing Zhou ◽  
Zhongbin Ye ◽  
Chun Wei ◽  
...  
Keyword(s):  
Sodium Dodecyl ◽  
Sodium Dodecyl Benzenesulfonate ◽  
Properties Of Composites

Tu1927 Matrix Metalloproteinase 9 (MMP9) Expression in Ulcerative Colitis Is Associated With Histologic Score of Disease Severity

2016 ◽  
Vol 150 (4) ◽  
pp. S979-S980
Author(s):  
William Sandborn ◽  
Scott Lee ◽  
Erik G. Huntzicker ◽  
Guang Chen ◽  
Victoria Smith ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Matrix Metalloproteinase ◽  
Disease Severity ◽  
Matrix Metalloproteinase 9 ◽  
Mmp9 Expression ◽  
Metalloproteinase 9

scholarly journals Anti-inflammatory Bifidobacterium strains prevent dextran sodium sulfate induced colitis and associated gut microbial dysbiosis in mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shashank Singh ◽  
Ruchika Bhatia ◽  
Pragyanshu Khare ◽  
Shikha Sharma ◽  
Sivasubramanian Rajarammohan ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Sodium Sulfate ◽  
Activity Index ◽  
Disease Activity Index ◽  
Dextran Sodium Sulfate ◽  
Raw 264.7 Cells ◽  
Spleen Weight ◽  
Lipocalin 2 ◽  
Weight Percentage ◽  
Microbial Dysbiosis

Abstract Crohn’s and ulcerative colitis are common inflammatory conditions associated with Inflammatory bowel disease. Owing to the importance of diet based approaches for the prevention of inflammatory gut conditions, the present study was aimed to screen the human isolates of Bifidobacterium strains based on their ability to reduce LPS-induced inflammation in murine macrophage (RAW 264.7) cells and to evaluate prioritized strains for their preventive efficacy against ulcerative colitis in mice. Twelve out of 25 isolated strains reduced the production of LPS-induced nitric oxide and inflammatory cytokines. Furthermore, three strains, B. longum Bif10, B. breve Bif11, and B. longum Bif16 conferred protection against dextran sodium sulfate induced colitis in mice. The three strains prevented shortening of colon, spleen weight, percentage body weight change and disease activity index relative to colitis mice. Lower levels of Lipocalin-2, TNF-α, IL-1β and IL-6 and improved SCFA levels were observed in Bifidobacterium supplemented mice relative to DSS counterparts. Bacterial composition of B. longum Bif10 and B. breve Bif11 fed mice was partly similar to the normal mice, while DSS and B. longum Bif16 supplemented mice showed deleterious alterations. At the genus level, Bifidobacterium supplementation inhibited the abundances of pathobionts such as Haemophilus, Klebsiella and Lachnospira there by conferring protection.

Molecular Weight and the Dodecyl Sulphate Binding of a Thylakoid Membrane Polypeptide Involved in a Reaction on the Oxygen-Evolving Side of Photosystem II

1977 ◽  
Vol 32 (5-6) ◽  
pp. 384-391 ◽  
Author(s):  
Hans Craubner ◽  
Friederike Koenig
Keyword(s):  
Molecular Weight ◽  
Thylakoid Membrane ◽  
Phosphate Buffer ◽  
Equilibrium Dialysis ◽  
Sodium Dodecyl ◽  
Spherical Shape ◽  
Apparent Molecular Weight ◽  
Partial Specific Volume ◽  
Experimental Conditions ◽  
Sodium Phosphate Buffer

Abstract The molecular weight of a thylakoid membrane polypeptide with the apparent molecular weight 11 000 was determined by measurement of the sedimentation velocity, the diffusion and the ef­fective partial specific volume. The molecular weight was found to be 6300 and that of the poly-peptide-dodecyl sulphate micelle was found to be 11 200. The frictional ratio was 1.35. In ad­dition, we determined the binding of dodecyl sulphate onto the polypeptide by equilibrium dialysis. We found that 1 g polypeptide binds 0.77 g sodium dodecyl sulphate which corresponds to 17 molecules dodecyl sulphate bound per polypeptide chain. In the absence of dodecyl sulphate the polypeptide aggregates. The molecular weights of the aggregates are in 0.01 м sodium phosphate buffer pH 7.2 150 000 and in a 1 :1 mixture of 0.01 м phosphate buffer and 96% ethanol 365 000. The frictional ratios were 1.07 and 1.16 respectively which points at a spherical shape. The experimental conditions for the determination of the dodecyl sulphate binding were critically scrutinised.

Lipocalin-2 and matrix metalloproteinase-9 expression in high-grade endometrial cancer and their prognostic value

2015 ◽  
Vol 67 (2) ◽  
pp. 206-215 ◽  
Author(s):  
Sanja Srdelić Mihalj ◽  
Ivana Kuzmić-Prusac ◽  
Sandra Zekić-Tomaš ◽  
Ivana Šamija-Projić ◽  
Vesna Čapkun
Keyword(s):  
Endometrial Cancer ◽  
Matrix Metalloproteinase ◽  
Prognostic Value ◽  
Matrix Metalloproteinase 9 ◽  
High Grade ◽  
Lipocalin 2 ◽  
Metalloproteinase 9

scholarly journals Involvement of TSP1 and MMP9/NGAL in Angiogenesis during Orthodontic Periodontal Remodeling

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Petra Surlin ◽  
Isabela Silosi ◽  
Anne Marie Rauten ◽  
Manole Cojocaru ◽  
Lili Foia
Keyword(s):  
Matrix Metalloproteinase ◽  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Good Control ◽  
Matrix Metalloproteinase 9 ◽  
Lipocalin 2 ◽  
Orthodontic Appliance ◽  
Bacterial Plaque ◽  
Crevicular Fluid ◽  
Metalloproteinase 9

In the present study the aim was to measure the levels of Thrombospondin-1 (TSP1) and Lipocalin-2/matrix metalloproteinase 9 (MMP9/NGAL) complex in gingival crevicular fluid (GCF) at different time points of orthodontic treatment, to determine the relationship between these values and those of total-matrix metalloproteinase 9 (MMP9) and theirs implication in angiogenesis balance, in the situation of a good control of the bacterial plaque, emphasizing the role of TSP1 and MMP9/NGAL complex. GCF samples were collected from 16 young orthodontic patients requiring upper canine distalization (test tooth) with first premolar extraction. The contralateral canine (control tooth) was free from orthodontic force. For the orthodontic appliance, brackets Roth 0.018 inch with 0.012 inch NiTi archwire and a laceback were used. TSP1, MMP9/NGAL, and MMP9 increased from 1 hour before activation of orthodontic appliance to a maximum at 8 hours for MMP9 and 72 hours for MMP9/NGAL and TSP1. The results show a change in time of TSP1, MMP9/NGAL, and MMP9 levels in GCF of patients with this method of orthodontic treatment. The powerful correlation of MMP9/NGAL with TSP1 suggests their stronger involvement in angiogenesis processes in PDL during orthodontic periodontal remodeling, in the situation of a healthy periodontium and a good control of the bacterial plaque.

Sign in / Sign up

Export Citation Format

Share Document