Aggregation Behavior of Acylated Pepsin-Solubilized Collagen Based on Fluorescence Spectrum Technology

2020 ◽  
Vol 74 (4) ◽  
pp. 391-399
Author(s):  
Conghu Li ◽  
Yan Wu ◽  
Guoying Li ◽  
Ying Zhang ◽  
Xinghong Ma ◽  
...  
Keyword(s):  
Fluorescence Spectrum ◽  
Hydrophobic Interaction ◽  
Ph Value ◽  
Correlation Spectroscopy ◽  
Aggregation Behavior ◽  
Temperature Dependent ◽  
Aggregation State ◽  
Molecular Collision ◽  
Tyrosine Fluorescence ◽  
Collagen Molecules

The aggregation behavior of collagen-based materials plays an important role in their processing because it could affect their physicochemical properties. Based on the intrinsic fluorescence characteristic of tyrosine, fluorescence spectrum technology was used to investigate the aggregation state of the acylated collagen molecules in aqueous solution. The results showed that the aggregate degree of the acylated collagen was higher than that of the native collagen due to the hydrophobic interaction. With the increase of concentrations of the acylated collagen or at NaCl higher than 40 mmol/L, the aggregate degree of the acylated collagen molecules increased. When the pH was close to the isoelectric point of the acylated collagen, the hydrophobic interaction and the hydrogen bond helped to increase the aggregation degree. However, with the increase of temperature (10–70 ℃), the aggregation state of the acylated collagen decreased gradually due to the quenching, the molecular collision, and the broken of hydrogen bonds. Furthermore, two-dimensional correlation spectroscopy (2D-COS) showed that the response order was 360 > 305 nm at various acylated collagen and NaCl (>40 mmol/L) concentrations, while the response order was 305 > 360 nm when the pH value was increased from 5.0 to 9.0. Temperature-dependent 2D-COS showed there were four bands that occurred and the response order was listed as follows: 293 > 305 > 360 > 420 nm. In brief, the results might provide an important guide for molding processes of the acylated collagen.

Temperature-Dependent Aggregation Behavior of Symmetric Long-Chain Bolaamphiphiles at the Air−Water Interface

Langmuir ◽  
2006 ◽  
Vol 22 (6) ◽  
pp. 2668-2675 ◽  
Author(s):  
Karen Köhler ◽  
Annette Meister ◽  
Bodo Dobner ◽  
Simon Drescher ◽  
Friederike Ziethe ◽  
...  
Keyword(s):  
Aggregation Behavior ◽  
Water Interface ◽  
Temperature Dependent ◽  
Air Water Interface ◽  
Long Chain

Two-Dimensional Infrared Spectroscopy and Principal Component Analysis Studies on a New Azobenzene Derivative Supramolecular System Based on Hydrogen Bonds

2003 ◽  
Vol 57 (8) ◽  
pp. 933-942 ◽  
Author(s):  
Yuqing Wu ◽  
Ya-Qiong Hao ◽  
Min Li ◽  
Chaowei Guo ◽  
Yukihiro Ozaki
Keyword(s):  
Principal Component Analysis ◽  
Hydrogen Bonds ◽  
Supramolecular Assembly ◽  
Principal Component ◽  
Correlation Spectroscopy ◽  
Supramolecular System ◽  
Two Dimensional ◽  
Temperature Dependent ◽  
2D Correlation Spectroscopy ◽  
2D Correlation Analysis

Infrared (IR) spectra of a supramolecular assembly with an azobenzene derivative and intermolecular hydrogen bonds have been measured in the temperature range from 30 to 200 °C to investigate heat-induced structural changes and thermal stability. Principal component analysis (PCA) and two kinds of two-dimensional (2D) correlation spectroscopy, variable–variable (VV) 2D and sample–sample (SS) 2D spectroscopy, have been employed to analyze the observed temperature-dependent spectral variations. The PCA and SS 2D correlation analyses have demonstrated that the complete decoupling of hydrogen bonds in the supramolecular assembly occurs between 110 and 115 °C, which is in good agreement with the results of a differential scanning calorimetry (DSC) study for the heating process. The PCA of the IR spectra in the region of 3600–3100 cm−1 has illustrated that there are at least four principal components for the different NH2 and CONH species in the present supramolecular system. The VV 2D correlation spectroscopy study has provided information about the structure and strength of hydrogen bonds of NH2 and CONH groups and their temperature-dependent variations. The different species of hydrogen-bonded NH2 and CONH groups in the supramolecular system can be clarified by the VV 2D correlation analysis. The VV 2D correlation analysis has also revealed the specific order of the temperature-induced changes in the hydrogen bonds of NH2 and CONH groups.

Controlled Hydrothermal Growth of CuO-Based Nanostructures from Copper Alginate

2011 ◽  
Vol 688 ◽  
pp. 19-22 ◽  
Author(s):  
Bing Bing Wang ◽  
Qing Shan Kong ◽  
Jian Ping Sun ◽  
Quan Feng Yu ◽  
Quan Ji ◽  
...  
Keyword(s):  
Hydrothermal Method ◽  
Growth Mechanism ◽  
Hydrothermal System ◽  
Ph Value ◽  
Structure Evolution ◽  
Structure Directing Agent ◽  
Aggregation State ◽  
Air Atmosphere ◽  
Morphology And Structure ◽  
Different Temperatures

Schistose and aciculate CuO nanostructures have been synthesized by a novel ammonia assisted hydrothermal method of copper alginate. The conversion processes of copper alginate are investigated by thermogravimetrics (TG) analyses under N2and air atmosphere. The morphology, structure, and composition of the obtained CuO are investigated using SEM,TEM and XRD. It is found that different temperature and pH value resulted in the morphology and structure evolution of CuO. Ammonia was used as structure-directing agent in the hydrothermal system. The aggregation state of the nanostructures was controlled by the temperature. Dispersive schistose structures about 1μm in diameter were synthesized with 0.5mL ammonia at different temperatures. Dispersive microspheres of about 4 μm in diameter were also synthesized with 1 mL ammonia. Microspheres composed of nanoneedles and nanoplates were synthesized at 120°C and 160°C, respectively. Moreover, a possible growth mechanism governing the formation of such a nanomicrostructure was primarily discussed.

Synthesis, Structure and Fluorescence Spectrum Studies of Bromocoumarin Derivants: C19H22BrNO6

2011 ◽  
Vol 396-398 ◽  
pp. 1864-1870 ◽  
Author(s):  
Ying Wang ◽  
Xiao Dong Liu ◽  
Zhuo Liu
Keyword(s):  
Fluorescence Spectrum ◽  
Title Compound ◽  
Ph Value ◽  
Cooling Water ◽  
Structural Data ◽  
Molecular Structures ◽  
Ammonium Bromide ◽  
Monoclinic System ◽  
Fluorescent Intensity ◽  
Ft Ir

The title compound has been prepared and characterized by P1PHNMR, FT-IR, fluorescence spectrum and thermal analysis. The crystal and molecular structures of dimethyl-(4-(7-acetoxyl-2H -chromen-2-one))-methyl-(2-acrylate)-ethyl-ammonium bromide were determined by X-ray diffraction methods. The compound crystallizes in Monoclinic system, space group C2/c, with lattice parameters a = 22.799(5), b =12.122(2), c =14.580(3) Å, β = 90.76(3)°, Mr = 440.29, V = 4029.1(14) ÅP3P, Z = 8, Dc= 1.452 g/cmP3P, F(000) = 1808, R = 0.0679 and wR = 0.1450. In the structure, The four N-C bond distances are in the range of 1.473(6) ~ 1.531(6) Å. The P1PHNMR and FT-IR data are in agreement with the structural data. The title compound also shows good fluorescent behaviors. The fluorescent intensity changed little at pH ranging from 7.5 to 9.5. The pH value of the circulation cooling water is in this range so that the title compound can be used in tracer detection.

Sign in / Sign up

Export Citation Format

Share Document