EXPRESS: Design Considerations for Discrete Frequency Infrared Microscopy Systems

2021 ◽  
pp. 000370282110133
Author(s):  
Rohit Bhargava ◽  
Yamuna Dilip Phal ◽  
Kevin Yeh
Keyword(s):  
Imaging System ◽  
Chemical Imaging ◽  
Optical Component ◽  
Imaging Systems ◽  
Imaging Data ◽  
Discrete Frequency ◽  
Figures Of Merit ◽  
Design Considerations ◽  
Hardware Implementations ◽  
Measurement Capabilities

Discrete frequency infrared (DFIR) chemical imaging is transforming the practice of microspectroscopy by enabling a diversity of instrumentation and new measurement capabilities. While a variety of hardware implementations have been realized, considerations in the design of all-IR microscopes have not yet been compiled. Here we describe the evolution of IR microscopes, provide rationales for design choices, and the major considerations for each optical component that together comprise an imaging system. We analyze design choices in illustrative examples that use these components to optimize performance, under their particular constraints. We then summarize a framework to assess the factors that determine an instrument’s performance mathematically. Finally, we summarize the design and analysis approach by enumerating performance figures of merit for spectroscopic imaging data that can be used to evaluate the capabilities of imaging systems or suitability for specific intended applications. Together, the presented concepts and examples should aid in understanding available instrument configurations, while guiding innovations in design of the next generation of IR chemical imaging spectrometers.

Correlation of Pore Structure and Permeability by SEM-Image Analysis

1990 ◽  
Vol 48 (1) ◽  
pp. 428-429
Author(s):  
C. A. Callender ◽  
Wm. C. Dawson ◽  
J. J. Funk
Keyword(s):  
Image Analysis ◽  
Pore Structure ◽  
Imaging System ◽  
Pore Space ◽  
Simulation Models ◽  
Image Analyzer ◽  
Imaging Data ◽  
Sem Images ◽  
Thin Sections ◽  
Rock Types

The geometric structure of pore space in some carbonate rocks can be correlated with petrophysical measurements by quantitatively analyzing binaries generated from SEM images. Reservoirs with similar porosities can have markedly different permeabilities. Image analysis identifies which characteristics of a rock are responsible for the permeability differences. Imaging data can explain unusual fluid flow patterns which, in turn, can improve production simulation models.Analytical SchemeOur sample suite consists of 30 Middle East carbonates having porosities ranging from 21 to 28% and permeabilities from 92 to 2153 md. Engineering tests reveal the lack of a consistent (predictable) relationship between porosity and permeability (Fig. 1). Finely polished thin sections were studied petrographically to determine rock texture. The studied thin sections represent four petrographically distinct carbonate rock types ranging from compacted, poorly-sorted, dolomitized, intraclastic grainstones to well-sorted, foraminiferal,ooid, peloidal grainstones. The samples were analyzed for pore structure by a Tracor Northern 5500 IPP 5B/80 image analyzer and a 80386 microprocessor-based imaging system. Between 30 and 50 SEM-generated backscattered electron images (frames) were collected per thin section. Binaries were created from the gray level that represents the pore space. Calculated values were averaged and the data analyzed to determine which geological pore structure characteristics actually affect permeability.

scholarly journals Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO2 treatment

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ghazal Azarfar ◽  
Ebrahim Aboualizadeh ◽  
Simona Ratti ◽  
Camilla Olivieri ◽  
Alessandra Norici ◽  
...  
Keyword(s):  
Algal Cell ◽  
Principal Component ◽  
External Perturbation ◽  
Time Lapse ◽  
Chemical Imaging ◽  
Hyperspectral Data ◽  
Aqueous Environment ◽  
Ir Absorption ◽  
Imaging Data ◽  
Mid Infrared

AbstractAlgae are the main primary producers in aquatic environments and therefore of fundamental importance for the global ecosystem. Mid-infrared (IR) microspectroscopy is a non-invasive tool that allows in principle studying chemical composition on a single-cell level. For a long time, however, mid-infrared (IR) imaging of living algal cells in an aqueous environment has been a challenge due to the strong IR absorption of water. In this study, we employed multi-beam synchrotron radiation to measure time-resolved IR hyperspectral images of individual Thalassiosira weissflogii cells in water in the course of acclimation to an abrupt change of CO2 availability (from 390 to 5000 ppm and vice versa) over 75 min. We used a previously developed algorithm to correct sinusoidal interference fringes from IR hyperspectral imaging data. After preprocessing and fringe correction of the hyperspectral data, principal component analysis (PCA) was performed to assess the spatial distribution of organic pools within the algal cells. Through the analysis of 200,000 spectra, we were able to identify compositional modifications associated with CO2 treatment. PCA revealed changes in the carbohydrate pool (1200–950 cm$$^{-1}$$ - 1 ), lipids (1740, 2852, 2922 cm$$^{-1}$$ - 1 ), and nucleic acid (1160 and 1201 cm$$^{-1}$$ - 1 ) as the major response of exposure to elevated CO2 concentrations. Our results show a local metabolism response to this external perturbation.

scholarly journals Effective processing and evaluation of chemical imaging data with respect to morphological features of the zebrafish embryo

2021 ◽  
Author(s):  
Katharina Halbach ◽  
Timothy Holbrook ◽  
Thorsten Reemtsma ◽  
Stephan Wagner
Keyword(s):  
Inductively Coupled Plasma ◽  
Zebrafish Embryo ◽  
Statistical Significance ◽  
Software Tool ◽  
Chemical Imaging ◽  
Imaging Data ◽  
Inductively Coupled ◽  
Spatially Resolved ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

AbstractA workflow was developed and implemented in a software tool for the automated combination of spatially resolved laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) data and data on the morphology of the biological tissue. Making use of a recently published biological annotation software, FishImager automatically assigns the biological feature as regions of interest (ROIs) and overlays them with the quantitative LA-ICP-MS data. Furthermore, statistical tools including cluster algorithms can be applied to the elemental intensity data and directly compared with the ROIs. This is effectively visualized in heatmaps. This allows gaining statistical significance on distribution and co-localization patterns. Finally, the biological functions of the assigned ROIs can then be easily linked with elemental distributions. We demonstrate the versatility of FishImager with quantitative LA-ICP-MS data of the zebrafish embryo tissue. The distribution of natural elements and xenobiotics is analyzed and discussed. With the help of FishImager, it was possible to identify compartments affected by toxicity effects or biological mechanisms to eliminate the xenobiotic. The presented workflow can be used for clinical and ecotoxicological testing, for example. Ultimately, it is a tool to simplify and reproduce interpretations of imaging LA-ICP-MS data in many applications. Graphical abstract

scholarly journals Матричное радиовидение на основе гетеродинного приема с применением радиолокации непрерывным излучением

2022 ◽  
Vol 48 (1) ◽  
pp. 47
Author(s):  
С.А. Королев ◽  
А.В. Горюнов ◽  
В.В. Паршин
Keyword(s):  
Continuous Wave ◽  
Angular Resolution ◽  
Imaging System ◽  
Imaging Systems ◽  
New Approach ◽  
Wave Radio ◽  
Radio Imaging ◽  
Wave Radar ◽  
Previous Level ◽  
Array Receiver

A new approach to the creation of millimeter-wave radio imaging systems is proposed. This approach is based on the use of an array receiver consisting of a densely packed (pixel size - 4 mm) array of planar mixers located in the focal plane of a quasi-optical objective, with application of the frequency-modulated continuous-wave radar technique. It has been demonstrated that the implementation of the heterodyne type of reception makes it possible to increase the distance range of the array radio imaging system up to ~ 100 m while maintaining the angular resolution at the previous level.

scholarly journals Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy

2019 ◽  
Author(s):  
Hesam Mazidi ◽  
Tianben Ding ◽  
Arye Nehorai ◽  
Matthew D. Lew
Keyword(s):  
Single Molecule ◽  
Computational Models ◽  
Amyloid Fibrils ◽  
Imaging System ◽  
Simulated Data ◽  
Super Resolution ◽  
Ground Truth ◽  
Localization Algorithm ◽  
Reconstruction Algorithms ◽  
Imaging Data

The resolution and accuracy of single-molecule localization micro-scopes (SMLMs) are routinely benchmarked using simulated data, calibration “rulers,” or comparisons to secondary imaging modalities. However, these methods cannot quantify the nanoscale accuracy of an arbitrary SMLM dataset. Here, we show that by computing localization stability under a well-chosen perturbation with accurate knowledge of the imaging system, we can robustly measure the confidence of individual localizations without ground-truth knowledge of the sample. We demonstrate that our method, termed Wasserstein-induced flux (WIF), measures the accuracy of various reconstruction algorithms directly on experimental 2D and 3D data of microtubules and amyloid fibrils. We further show that WIF confidences can be used to evaluate the mismatch between computational models and imaging data, enhance the accuracy and resolution of recon-structed structures, and discover hidden molecular heterogeneities. As a computational methodology, WIF is broadly applicable to any SMLM dataset, imaging system, and localization algorithm.

scholarly journals Spatial Chemometrics and Comprehensive Chemical Imaging based Molecular Histopathology Delineates Anatomical Heterogeneity at Single Pixel Resolution

2020 ◽  
Author(s):  
Patrick Wehrli ◽  
Wojciech Michno ◽  
Laurent Guerard ◽  
Julia Fernandez-Rodriguez ◽  
Anders Bergh ◽  
...  
Keyword(s):  
Image Registration ◽  
Biological Tissues ◽  
Chemical Imaging ◽  
Chemical Information ◽  
Imaging Data ◽  
Pixel Resolution ◽  
Registration Method ◽  
Analysis Methods ◽  
Multiple Imaging ◽  
Single Pixel

<p>Imaging mass spectrometry (IMS) is a powerful tool for spatially-resolved chemical analysis and thereby offers novel perspectives for applications in biology and medicine. The understanding of chemically complex systems, such as biological tissues, benefits from the combination of multiple imaging modalities contributing with complementary molecular information. Effective analysis and interpretation of multimodal IMS data is challenging and requires both, precise alignment and combination of the imaging data as well as suitable statistical analysis methods to identify cross-modal correlations. Commonly applied IMS data analysis methods include qualitative comparative analysis where cross-modal interpretation is subject to human judgement; Workflows that incorporate image registration procedures are usually applied for co-representing data rather than to mine data across modalities. </p><p>Here, we present an IMS-based, histology-driven strategy for comprehensive interrogation of biological tissues by spatial chemometrics. Our workflow implements a 1+1-evolutionary image registration method enabling direct correlation of chemical information across multiple modalities at single pixel resolution. Comprehensive multimodal imaging data were evaluated using a novel approach based on orthogonal multiblock component analysis (OnPLS). Finally, we present a novel image fusion method by implementing consecutively acquired pathological staining data to enhance histological interpretation.</p><p>We demonstrate the method’s potential in two biomedical applications where trimodal matrix-assisted laser desorption/ionization (MALDI) IMS delineates pathology associated co-localization patterns of lipids and proteins in (1) a transgenic Alzheimer’s disease (AD) mouse model, and in (2) a human xenograft rat model of prostate cancer. The presented image analysis paradigm allows to comprehensively interrogate complex biological systems with single pixel resolution at cellular length scales.</p>

Performance Enhancement of Gain-Modulated 3D Imaging System by Multi-Pulse Accumulation

2013 ◽  
Vol 475-476 ◽  
pp. 259-262
Author(s):  
Fei Wang
Keyword(s):  
3D Imaging ◽  
Performance Enhancement ◽  
Imaging System ◽  
Imaging Systems ◽  
Laser Imaging ◽  
Range Error ◽  
Accumulation Method ◽  
Accuracy Performance

Range accuracy is one of the key parameters for 3D laser imaging systems. A gain-modulated 3D imaging system employing multi-pulse accumulation method is proposed to improve the range accuracy performance. Experiments results show that the range error decreases exponentially with the number of accumulated laser echoes.

Imaging Systems in Search and Rescue: Implications for Geographic Orientation

2002 ◽  
Vol 46 (1) ◽  
pp. 170-174
Author(s):  
Jocelyn Keillor ◽  
Karen J. Hodges ◽  
Michael Perlin ◽  
Nada Ivanovic ◽  
J.G. Hollands
Keyword(s):  
Optical Imaging ◽  
Imaging System ◽  
Frame Of Reference ◽  
Search And Rescue ◽  
Imaging Systems ◽  
Terrain Model ◽  
Optical Imaging System ◽  
Traditional Process

Optical imaging systems have the potential to dramatically change the task of a search and rescue technician. An important difference between the traditional process of “looking out the window” and search conducted with the aid of an optical imaging system is that in the case of imaging systems the frame of reference for the viewed display is de-coupled from the technician's frame of reference. We examined the ability of a moving-map display that recorded the locations of designated targets to support geographic orientation in operators with and without knowledge of the modeled terrain. Participants who did not have knowledge of the terrain benefited from the moving map, as when it was present they were less likely to re-identify targets that they had already viewed, whereas those who were already familiar with the terrain model showed no benefit from this manipulation. Both groups had difficulty localizing targets on a map following flight, and the two groups did not differ in their ability to initially detect targets using the system.

Development of Real Time High Energy X-Ray Imaging System for Use in Dynamic Fluoroscopy of Aero Gas Turbines

1975 ◽  
Author(s):  
A. E. Stewart
Keyword(s):  
Real Time ◽  
Gas Turbines ◽  
Imaging System ◽  
High Energy ◽  
Cesium Iodide ◽  
Imaging Systems ◽  
X Ray ◽  
X Ray Imaging ◽  
Different Types

This paper discusses the development of a real-time high energy x-ray imaging system for use in dynamic fluoroscopy of aero gas turbines. In order to cover the range of subjects on gas turbines, over ten combinations of film and screen types are used. Three different types of x-ray imaging systems were considered for use: direct type intensifiers (cesium iodide phosphors), and indirect type intensifiers — Marconi “Marionette” and the Oude Delft “Delcalix.”

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