Histologic Study of Homograft Cartilages Implanted in the Middle Ear

We examined conditions of the micro-sliced homograft cartilages implanted in the middle ear, implanted cartilages removed at revision surgery or implanted cartilages removed at the second stage of staged tympanoplasty, both macroscopically and histologically. Macroscopically, the appearance and shape of the cartilages remained unchanged, with no evidence of erosion. There was no evidence of any foreign body reaction or rejection phenomenon. In general, no marked histologic changes of the matrix tissues were found, although chondrocytes showed degenerative changes. There was partial absorption of cartilage and replacement by fibrous connective tissue when inflammatory changes occurred in the middle ear. It is concluded that implanted homograft cartilage maintains its stiffness for more than 6 months in a healthy, aerated middle ear and appears to be clinically useful for tympanoplasty.

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Should we be concerned about histologic changes caused by novel submucosal injection agents used for EMR and ESD?

@Gijournal ◽

10.52697/gijournal.v1i1.20 ◽

2021 ◽

Vol 1 ◽

Author(s):

Daniyal Abbas ◽

Emma Furth ◽

Pallavi Patil ◽

Nirag Jhala ◽

Dmitriy Kedrin

Keyword(s):

Foreign Body ◽

Endoscopic Resection ◽

Normal Saline ◽

Foreign Body Reaction ◽

Novel Agents ◽

Pathologic Finding ◽

Submucosal Injection ◽

Histologic Appearance ◽

Histologic Changes ◽

Appearance Changes

Normal saline mixed with dyes is a widely used submucosal lifting agent for endoscopic resection of small gastrointestinal (GI) lesions. For larger GI lesions, novel agents are used to achieve optimal results. ORISE gel is a novel, premixed submucosal lifting solution for endoscopic resection procedures. The initial histologic appearance of endoscopic specimens with injection of ORISE gel is characterized by a pale, basophilic amorphous appearance which has the potential to be misinterpreted as a pathologic finding. The histologic appearance changes overtime to an eosinophilic appearance that is reflected by an inflammatory, foreign body reaction. We summarize the @GIJournal discussion held on May 12, 2021, about the article by Olivas et al “Histologic changes caused injection of a novel submucosal lifting agent for endoscopic resection in GI lesions” 1. The guest experts to discuss pathology included Drs. Emma Furth (EF), and Pallavi Patil (PP), the discussion was moderated by Dr. Dmitriy Kedrin (DK).

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Peritoneal Inflammation after Twenty-Week Exposure to Dialysis Solution: Effect of Solution versus Catheter–Foreign Body Reaction

Peritoneal Dialysis International ◽

10.3747/pdi.2009.00100 ◽

2010 ◽

Vol 30 (3) ◽

pp. 284-293 ◽

Cited By ~ 28

Author(s):

Michael F. Flessner ◽

Kimberly Credit ◽

Karla Richardson ◽

Rebecca Potter ◽

Xiaorong Li ◽

...

Keyword(s):

Foreign Body ◽

Inflammatory Markers ◽

Foreign Body Reaction ◽

Ethyl Pyruvate ◽

Vascular Density ◽

Hypertonic Solution ◽

Adherent Cells ◽

Peritoneal Dialysis Catheter ◽

Peritoneal Inflammation ◽

Inflammatory Changes

♦ BackgroundWe hypothesized that both sterile solutions and foreign body reaction to the peritoneal dialysis catheter are associated with inflammatory changes in rats exposed to hypertonic solution.♦ MethodsFour hypertonic solutions (30 – 40 mL) were injected daily via needle and syringe over 20 weeks in 4 groups of rats: 4.25% standard clinical solution (LAC), LAC plus pyridoxamine (PYR), LAC plus ethyl pyruvate (EP), and a biocompatible 4% dextrose solution (BIC). Two groups received catheters: a non-injected 4-week catheter group (C4) and a group injected for 20 weeks with the BIC solution (CI). Control animals (CON) were not injected. In the C4 group, adherent cells were separated from the catheter and examined by culture and electron microscopy to ensure that animals were bacteria free prior to exposure to solution. Animals underwent transport experiments to determine mass transfer coefficients of mannitol (MTCM) and albumin (MTCA), osmotic filtration flux (Josm), and hydrostatic pressure-driven flux (Jp). After euthanasia, tissues were examined for submesothelial thickness, vascular density, and immunohistochemistry for various cytokines.♦ ResultsThe catheter cell layer was free of bacteria and consisted of macrophages, lymphocytes, mesothelial cells, and fibroblastic cells. Marked differences in angiogenesis and submesothelial thickening were noted for the catheter groups. Transport differences were mixed: MTCMwas significantly less for the CI group and MTCAwas variable among the groups. There were no differences among groups for Josmor Jp. Inflammatory markers in the catheter-adherent cells correlated with inflammatory changes in the tissue. These data demonstrate significant changes in submesothelial thickness, angiogenesis, transport function, and inflammatory markers between animals injected with sterile solutions over 20 weeks with and without catheters.♦ ConclusionsAn indwelling catheter amplifies peritoneal inflammation from dialysis solutions through a foreign body reaction. Our data also suggest that additives to existing solutions may have limited the effect on inflammatory response to non-biocompatible solutions.

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Inflammatory Reaction as Determinant of Foreign Body Reaction Is an Early and Susceptible Event after Mesh Implantation

BioMed Research International ◽

10.1155/2014/510807 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 14

Author(s):

Holger Gerullis ◽

Evangelos Georgas ◽

Mihaly Borós ◽

Bernd Klosterhalfen ◽

Christoph Eimer ◽

...

Keyword(s):

Foreign Body ◽

Connective Tissue ◽

Inflammatory Reaction ◽

Inflammatory Infiltrate ◽

Foreign Body Reaction ◽

Plasma Coating ◽

Mesh Implantation ◽

Long Term Follow Up ◽

Infiltrate Macrophage

Purpose.To investigate and relate the ultrashort-term and long-term courses of determinants for foreign body reaction as biocompatibility predictors for meshes in an animal model.Materials and Methods.Three different meshes (TVT, UltraPro, and PVDF) were implanted in sheep. Native and plasma coated meshes were placed bilaterally: (a) interaperitoneally, (b) as fascia onlay, and (c) as muscle onlay (fascia sublay). At 5 min, 20 min, 60 min, and 120 min meshes were explanted and histochemically investigated for inflammatory infiltrate, macrophage infiltration, vessel formation, myofibroblast invasion, and connective tissue accumulation. The results were related to long-term values over 24 months.Results.Macrophage invasion reached highest extents with up to 60% in short-term and decreased within 24 months to about 30%. Inflammatory infiltrate increased within the first 2 hours, the reached levels and the different extents and ranking among the investigated meshes remained stable during long-term follow up. For myofibroblasts, connective tissue, and CD31+ cells, no activity was detected during the first 120 min.Conclusion.The local inflammatory reaction is an early and susceptible event after mesh implantation. It cannot be influenced by prior plasma coating and does not depend on the localisation of implantation.

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Modulation of MNC Phenotype and Macrophage Differentiation by the Matrix Elasticity in the Foreign Body Reaction

SSRN Electronic Journal ◽

10.2139/ssrn.3674114 ◽

2020 ◽

Author(s):

Josephine Y. Fang ◽

Zhi Yang ◽

Wei Hu ◽

Ba Xuan Hoang ◽

Bo Han

Keyword(s):

Foreign Body ◽

Foreign Body Reaction ◽

Macrophage Differentiation ◽

Matrix Elasticity ◽

The Matrix

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AN EXPERIMENTAL STUDY OF FOREIGN BODY REACTION IN THE MIDDLE EAR

Nippon Jibiinkoka Gakkai Kaiho ◽

10.3950/jibiinkoka.65.723 ◽

1962 ◽

Vol 65 (6) ◽

pp. 723-729

Author(s):

T. GOTO ◽

H. KUMAGAMI

Keyword(s):

Experimental Study ◽

Foreign Body ◽

Middle Ear ◽

Foreign Body Reaction

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Atelectatic Tympanic Membrane: Histologic Study

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348949310200912 ◽

1993 ◽

Vol 102 (9) ◽

pp. 712-716 ◽

Cited By ~ 52

Author(s):

Jacob Sadé

Keyword(s):

Tympanic Membrane ◽

Middle Ear ◽

Atmospheric Pressure ◽

Morphological Difference ◽

Histologic Examination ◽

Histologic Study ◽

Pars Tensa ◽

The Difference ◽

Inflammatory Changes

This study aims to elucidate the morphological difference between the noncollapsible physiologic tympanic membrane (TM) and the collapsible atelectatic TM. Histologic examination of atelectatic TMs obtained at surgery from 16 ears revealed inflammatory changes and destruction of the pars tensa collagenous “backbone.” The disappearance of the organized collagenous layer seen in atelectatic TMs explains their conversion from the stiff physiologic membrane to a flexible one. This in turn will convert the middle ear from a noncollapsible gas pocket to a partially collapsible gas pocket, in which minimal pressure of only a few millimeters of water can cause retraction or ballooning of the atrophic TM. These pressure differences are too small to equilibrate automatically the difference created with the atmospheric pressure. Integrity of the pars tensa collagenous layer thus appears to be essential for the proper mechanical functioning of the physiologic TM.

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COMPARATIVE HISTOLOGIC STUDY IN ENDOMETRIUM OF PREGNANT AND COWS WITH SYMPTOM FREE STERILITY

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.20136 ◽

1974 ◽

Vol 25 (1) ◽

pp. 19

Author(s):

Κ. ΣΕΪΤΑΡΙΔΗΣ ◽

Α. ΡΑΝΤΣΙΟΣ ◽

Β. ΕΛΕΖΟΓΛΟΥ

Keyword(s):

Connective Tissue ◽

Plasma Cell ◽

Luteal Phase ◽

Tissue Development ◽

Histologic Study ◽

Inflammatory Changes ◽

Lymphocyte Cell

Histologic examinate of the endometrium of 37 cows with symptom free sterility (21 during folicular and 16 during luteal phase) and the endometrium of 26 pregnant cows (1st - 4th month of pregnancy) demonstrated that 94.6 % of the former and 80,8 % of the latter show various degrees of inflammatory changes. These changes were mainly plasma cell and lymphocyte cell infiltrations particularly during the folicular phase (P<0,001 versus pregnant cows endometrium), inflammatory perivascular infiltrations and of the glands of endometrium, particularly during the luteal phase (P<0,01 versus pregnant cows) and connective tissue development during pregnancy (P<0,01).

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Traumatic Exogenous Uveitis

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.1055/s-0038-1633160 ◽

1988 ◽

Vol 01 (01) ◽

pp. 35-37 ◽

Cited By ~ 1

Author(s):

B. P. Wilcock ◽

Melanie M. Williams

Keyword(s):

Weight Loss ◽

Foreign Body ◽

Ciliary Body ◽

Foreign Body Reaction ◽

Anterior Segment ◽

Tissue Formation ◽

Ocular Tissues ◽

Granulation Tissue Formation ◽

Orbital Muscle ◽

Inflammatory Changes

A young dog was presented to the veterinary ophthalmology service with marked unilateral anterior uveitis and blindness, extreme pain, anorexia and weight loss. Porcupine quill migration into the orbital and ocular tissues was suspected due to an encounter with a porcupine three weeks previously. Surgical orbital extenteration and exploration revealed two porcupine quill segments penetrating the oculus bulbi and extending into the orbital tissues. The location and movement of the quills within the globe and orbital muscle cone during mastication accounted for the deep pain, anorexia and weight loss. The quills incited a sterile foreign body reaction with fibroplasia and granulation tissue formation in the sclera and ciliary body. The eye was avisual due to the anterior segment structural and inflammatory changes.

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Meconium aspiration pneumonia and otitis media in two goat kids

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638719834602 ◽

2019 ◽

Vol 31 (3) ◽

pp. 463-466

Author(s):

Shelley J. Newman ◽

Olufemi O. Fasina

Keyword(s):

Foreign Body ◽

Otitis Media ◽

Aspiration Pneumonia ◽

Eustachian Tube ◽

Middle Ear ◽

Foreign Body Reaction ◽

Plasma Cells ◽

Meconium Aspiration ◽

Squamous Cells ◽

Rare Lesion

We report meconium aspiration in 2 sibling goat kids, and characterize the expected lesions of aspiration pneumonia in conjunction with the rare lesion of otitis media. Grossly, the lungs were multifocally consolidated, and there was yellow-green exudate within the middle ear. Histologically, the lung was characterized by pyogranulomatous pneumonia and foreign-body reaction around aspirated debris. Within the lumen of the middle ear, aspirated squamous cells, keratin, meconium debris, and neutrophils, without evidence of bacteria, were accompanied by a subepithelial accumulation of lymphocytes, plasma cells, and fewer macrophages. This is an especially rare phenomenon, which is thought to result from transport of meconium from the oropharynx through the auditory tube (Eustachian tube) to the middle ear.

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