p53 Expression in Canine Liposarcoma Correlates With Myxoid Variant and Higher Proliferative Activity

Canine liposarcoma is classified as well differentiated (WDL), dedifferentiated (DDL), myxoid (ML), and pleomorphic (PL). Overexpression of the protooncogene MDM2 has been reported in WDL and DDL, but little is known regarding the role of p53 in their tumorigenesis. The aim of this study was to assess p53 expression in canine liposarcoma and compare it with subtype, grade, mitotic count (MC), Ki67 labeling index (LI), and MDM2 expression. Forty-seven cases were included (13 WDL, 3 DDL, 7 ML, and 24 PL); 17 were MDM2-positive (13 WDL, 3DDL, and 1ML). Five were p53-positive (4 ML and 1 WDL) but DDL and PL were consistently negative. p53 expression correlated with higher Ki67-LI, higher MC, and myxoid histotype. No correlation was found with grade and MDM2 expression. Based on these results canine liposarcoma seems to embody a group of neoplasms whose subtypes, especially ML, may represent distinct diseases rather than morphological variants of the same entity.

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Effect of Tumor Heterogeneity on the Assessment of Ki67 Labeling Index in Well-differentiated Neuroendocrine Tumors Metastatic to the Liver

The American Journal of Surgical Pathology ◽

10.1097/pas.0b013e31821a0696 ◽

2011 ◽

Vol 35 (6) ◽

pp. 853-860 ◽

Cited By ~ 188

Author(s):

Zhaohai Yang ◽

Laura H. Tang ◽

David S. Klimstra

Keyword(s):

Neuroendocrine Tumors ◽

Tumor Heterogeneity ◽

Labeling Index ◽

Ki67 Labeling Index ◽

Well Differentiated

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High Inter- and Intratumoral Variability of Ki67 Labeling Index in Newly Diagnosed Prostate Cancer with High Gleason Scores

Pathobiology ◽

10.1159/000519007 ◽

2021 ◽

pp. 1-7

Author(s):

Tatjana Vlajnic ◽

Patrik Brunner ◽

Serenella Eppenberger-Castori ◽

Cyrill A. Rentsch ◽

Tobias Zellweger ◽

...

Keyword(s):

Gleason Score ◽

Treatment Strategies ◽

Progression Free Survival ◽

Labeling Index ◽

High Grade ◽

Newly Diagnosed ◽

Ki67 Labeling Index ◽

The Status ◽

Underlying Mechanisms

Background: The majority of studies investigating the role of Ki67 labeling index (LI) in prostate carcinoma (PC) focused on localized PC treated radically, where Ki67 LI is regarded as a prognostic marker. The relevance of Ki67 in advanced PC remains largely unexplored. While Gleason score is still one of the best indicators of clinical outcomes in PC, differences in progression-free survival and overall survival in patients with high Gleason scores suggest that additional factors are involved in tumor progression. Understanding the underlying mechanisms could help to optimize treatment strategies for an individual patient. Here, we aimed to determine the inter- and intratumoral distribution of Ki67 LI in patients with PC with high Gleason scores and to correlate Ki67 LI with the status of ERG, PTEN, and Bcl-2. Methods: Immunohistochemistry for Ki67, ERG, PTEN, and Bcl-2 was performed on core needle biopsies from 112 patients with newly diagnosed PC Gleason score 8, 9, and 10. Results: Using a cutoff of ≥10%, 17/112 cases (15%) had a homogeneously low and 95/112 cases (85%) a high Ki67 LI. 41% of cases showed intratumoral heterogeneity containing areas with low and high proliferation. There was no association between Ki67 LI and ERG, PTEN, or Bcl-2 status. Conclusions: Our data demonstrate major inter- and intratumoral variability of Ki67 LI in high-grade PC with a surprisingly low Ki67 LI in a subset of cases. Further studies are necessary to explore the molecular basis and potential clinical implications of a paradoxically low proliferation rate in high-grade PC.

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Canine smooth muscle tumors: A clinicopathological study

Veterinary Pathology ◽

10.1177/03009858211066862 ◽

2021 ◽

pp. 030098582110668

Author(s):

Giancarlo Avallone ◽

Valeria Pellegrino ◽

Luisa Vera Muscatello ◽

Paola Roccabianca ◽

Gastone Castellani ◽

...

Keyword(s):

Smooth Muscle ◽

Soft Tissue ◽

Genital Tract ◽

Female Genital Tract ◽

Mitotic Count ◽

Labeling Index ◽

Ki67 Labeling Index ◽

Smooth Muscle Tumors ◽

Criteria Of Malignancy ◽

Female Genital

Canine smooth muscle tumors (SMTs) commonly develop in the alimentary and female genital tracts and less frequently in soft tissue. The definition of histological criteria of malignancy is less detailed for SMTs in dogs than in humans. This study evaluated the clinicopathologic features of canine SMTs and compared the veterinary and human medical criteria of malignancy. A total of 105 canine SMTs were evaluated histologically and classified according to both veterinary and human criteria. The Ki67 labeling index was assessed in all SMTs. Estrogen receptor (ER) and progesterone receptor (PR) expression was evaluated for soft tissue SMTs. Follow-up data were available in 25 cases. SMTs were diagnosed in the female genital tract (42%), alimentary tract (22%), and soft tissue (20%). Soft tissue SMTs frequently arose in the perigenital area, pelvic cavity, and retroperitoneum. A subset of soft tissue SMTs expressed ER and/or PR, resembling the gynecologic type of soft tissue SMT in humans. SMTs were less frequently malignant when assessed with human criteria than with veterinary criteria, better reflecting their benign behavior, especially in the genital tract where human criteria tolerate a higher mitotic count for leiomyoma. Decreased differentiation was correlated with increased proliferation, necrosis, and reduced desmin expression. Mitotic count, Ki67 labeling index, and necrosis were correlated with metastases and tumor-related death. Further prognostic studies are warranted to confirm the better performance of the human criteria when assessing SMT malignancy, especially genital cases, to confirm their usefulness in ER/PR-expressing soft tissue SMTs, and to better define the most useful prognostic parameters for canine SMTs.

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Role of Ki67 labeling index (LI) in subdividing homogeneous ER-positive grading groups of early breast cancer (EBC) in distinct biologic entities.

Journal of Clinical Oncology ◽

10.1200/jco.2011.29.15_suppl.539 ◽

2011 ◽

Vol 29 (15_suppl) ◽

pp. 539-539

Author(s):

A. Ferro ◽

M. Barbareschi ◽

C. Eccher ◽

A. Caldara ◽

D. Aldovini ◽

...

Keyword(s):

Breast Cancer ◽

Early Breast Cancer ◽

Labeling Index ◽

Ki67 Labeling Index ◽

Er Positive

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Role of Proliferative Activity Estimated by Bromodeoxyuridine Labeling Index in Determining Predictive Factors of Recurrence in Superficial Intermediately Malignant Bladder Tumors

The Journal of Urology ◽

10.1097/00005392-199607000-00018 ◽

1996 ◽

pp. 63-69 ◽

Cited By ~ 1

Author(s):

Masaaki Tachibana ◽

Ayako Miyakawa ◽

Kaoru Nakamura ◽

Shiro Baba ◽

Masaru Murai ◽

...

Keyword(s):

Predictive Factors ◽

Proliferative Activity ◽

Labeling Index ◽

Bladder Tumors

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Role of Proliferative Activity Estimated by Bromodeoxyuridine Labeling Index in Determining Predictive Factors of Recurrence in Superficial Intermediately Malignant Bladder Tumors

The Journal of Urology ◽

10.1016/s0022-5347(01)65940-8 ◽

1996 ◽

Vol 156 (1) ◽

pp. 63-69 ◽

Cited By ~ 6

Author(s):

Masaaki Tachibana ◽

Ayako Miyakawa ◽

Kaoru Nakamura ◽

Shiro Baba ◽

Masaru Murai ◽

...

Keyword(s):

Predictive Factors ◽

Proliferative Activity ◽

Labeling Index ◽

Bladder Tumors

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Correlation between p53 and Mdm2 expression with histopathological parameters in cattle squamous cell carcinomas

Veterinary World ◽

10.14202/vetworld.2022.10-15 ◽

2022 ◽

pp. 10-15

Author(s):

Waseem Al-Jameel ◽

S. S. Al-Mahmood ◽

A. M. Al-Saidya

Keyword(s):

Squamous Cell ◽

Histological Grading ◽

P53 Expression ◽

Grading Systems ◽

Mdm2 Expression ◽

Poorly Differentiated ◽

Mitotic Figures ◽

Degree Of Differentiation ◽

Histopathological Grading

Background and Aim: Squamous cell carcinoma (SCC) is the most common form of carcinoma in cattle. Histopathological grading systems have been utilized over several decades for estimating the malignancy of cattle SCCs. This study aimed to detect p53 and Mdm2 expression in different SCC cases in cattle and correlate their expression with the SCC histopathological grading. Materials and Methods: Cattle SCC cases were collected at the Veterinary Teaching Hospital in Nineveh. The SCC grading system categorized the cases histologically based on their differentiation grade into three groups: Well, moderately, and poorly differentiated. The SCC cases were subsequently verified for p53 and Mdm2 immunoexpression. Results: Fourteen of 16 examined cattle SCC samples tested positive for p53 expression. Moreover, 15 out of the 16 SCC samples tested positive for Mdm2 expression. The increased immunoreactivity of both p53 and Mdm2 was associated with a poor histological grading of the cattle SCC. There is a positive correlation between the nuclear expression of p53 and Mdm2, and the degree of differentiation and the number of mitotic figures in the examined cattle SCC samples. Conclusion: Our results demonstrate an increased p53 and Mdm2 expression in cattle SCC cases characterized by poor histopathological grading, thus suggesting an essential role of these molecules in the development of moderately and poorly differentiated SCC in cattle.

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