scholarly journals Correlation between p53 and Mdm2 expression with histopathological parameters in cattle squamous cell carcinomas

2022 ◽  
pp. 10-15
Author(s):  
Waseem Al-Jameel ◽  
S. S. Al-Mahmood ◽  
A. M. Al-Saidya
Keyword(s):  
Squamous Cell ◽  
Histological Grading ◽  
P53 Expression ◽  
Grading Systems ◽  
Mdm2 Expression ◽  
Poorly Differentiated ◽  
Mitotic Figures ◽  
Degree Of Differentiation ◽  
Histopathological Grading

Background and Aim: Squamous cell carcinoma (SCC) is the most common form of carcinoma in cattle. Histopathological grading systems have been utilized over several decades for estimating the malignancy of cattle SCCs. This study aimed to detect p53 and Mdm2 expression in different SCC cases in cattle and correlate their expression with the SCC histopathological grading. Materials and Methods: Cattle SCC cases were collected at the Veterinary Teaching Hospital in Nineveh. The SCC grading system categorized the cases histologically based on their differentiation grade into three groups: Well, moderately, and poorly differentiated. The SCC cases were subsequently verified for p53 and Mdm2 immunoexpression. Results: Fourteen of 16 examined cattle SCC samples tested positive for p53 expression. Moreover, 15 out of the 16 SCC samples tested positive for Mdm2 expression. The increased immunoreactivity of both p53 and Mdm2 was associated with a poor histological grading of the cattle SCC. There is a positive correlation between the nuclear expression of p53 and Mdm2, and the degree of differentiation and the number of mitotic figures in the examined cattle SCC samples. Conclusion: Our results demonstrate an increased p53 and Mdm2 expression in cattle SCC cases characterized by poor histopathological grading, thus suggesting an essential role of these molecules in the development of moderately and poorly differentiated SCC in cattle.

The prognostic value of histopathological grading systems in oral squamous cell carcinomas

Oral Diseases ◽  
2015 ◽  
Vol 21 (6) ◽  
pp. 755-761 ◽  
Author(s):  
I Sawazaki-Calone ◽  
ALCA Rangel ◽  
AG Bueno ◽  
CF Morais ◽  
HM Nagai ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Prognostic Value ◽  
Squamous Cell Carcinomas ◽  
Oral Squamous Cell Carcinomas ◽  
Grading Systems ◽  
Histopathological Grading

p53 Expression in Canine Liposarcoma Correlates With Myxoid Variant and Higher Proliferative Activity

2020 ◽  
Vol 57 (5) ◽  
pp. 620-622
Author(s):  
Giancarlo Avallone ◽  
Luisa V. Muscatello ◽  
Alessandro Leoni ◽  
Paola Roccabianca ◽  
Elvio Lepri ◽  
...  
Keyword(s):  
Proliferative Activity ◽  
Mitotic Count ◽  
Labeling Index ◽  
P53 Expression ◽  
Ki67 Labeling Index ◽  
Mdm2 Expression ◽  
Morphological Variants ◽  
Well Differentiated

Canine liposarcoma is classified as well differentiated (WDL), dedifferentiated (DDL), myxoid (ML), and pleomorphic (PL). Overexpression of the protooncogene MDM2 has been reported in WDL and DDL, but little is known regarding the role of p53 in their tumorigenesis. The aim of this study was to assess p53 expression in canine liposarcoma and compare it with subtype, grade, mitotic count (MC), Ki67 labeling index (LI), and MDM2 expression. Forty-seven cases were included (13 WDL, 3 DDL, 7 ML, and 24 PL); 17 were MDM2-positive (13 WDL, 3DDL, and 1ML). Five were p53-positive (4 ML and 1 WDL) but DDL and PL were consistently negative. p53 expression correlated with higher Ki67-LI, higher MC, and myxoid histotype. No correlation was found with grade and MDM2 expression. Based on these results canine liposarcoma seems to embody a group of neoplasms whose subtypes, especially ML, may represent distinct diseases rather than morphological variants of the same entity.

The Significant Impact of Immunohistochemistry in the Classification of Lung Carcinoma on Small Biopsies

2021 ◽  
Vol 8 (3) ◽  
pp. 60-65
Author(s):  
Debjani Mallick ◽  
Sayan Kundu ◽  
Sudipta Chakrabarti ◽  
Prosun Gayen
Keyword(s):  
Cell Carcinoma ◽  
Squamous Cell ◽  
Lung Carcinoma ◽  
Clinical Findings ◽  
Small Cell ◽  
Napsin A ◽  
Morphological Diagnosis ◽  
Poorly Differentiated ◽  
Undifferentiated Tumors

Background : There are limitations of histomorphology in the appropriate categorization of lung carcinoma where immunohistochemistry can confirm the morphological diagnosis and may add value in the poorly differentiated and undifferentiated tumors. The aim of the study was to assess the role of immunohistochemistry in classifying lung carcinoma on small biopsy samples. Methods and Material: A retrospective hospital based, observational study was conducted on cases of lung carcinoma diagnosed by core needle or bronchoscopic biopsies over a 3- year period. After evaluation of clinical findings and H&E sections, all biopsies were evaluated by immunohistochemical staining. The immunohistochemistry panel included cytokeratin cocktail, CK7, CK 20, TTF1, Napsin A, CK5/6, p40, synaptophysin, chromogranin, CD 56. Result: Out of 78 cases, the mean age was 58 +/- 11 years. Most prevalent malignancy type was adenocarcinoma (30, 38.1%). Adenocarcinoma cases were positive for CK7 (25/26, 96%), Napsin A (24/26, 92%), TTF1 (15/30, 50%) and negative for CK 20. Squamous cell carcinoma cases showed positivity for p40(18/22, 82%) and CK 5/6 (17/22.71%).Small cell carcinoma showed positivity for neuroendocrine markers synaptophysin (5/7,71%) and chromogranin(4/7, 57%) and CD 56 (6/7cases (85%).88% of small cell carcinomas,75% of adenocarcinomas and 72 % of squamous cell carcinomas were accurately diagnosed by morphology. Morphologic prediction was poor in the NSCC NOS group (0%) and poorly differentiated carcinomas (64%), which were finally, diagnosed by immunohistochemistry.In the morphologically diagnosed cases, immunohistochemistry confirmed the diagnosis. Conclusion: Thus, morphology added with immunohisto chemistry provides a crisp diagnosis thereby improving therapeutic efficacy

Leiomyosarcoma of the urinary bladder: a clinicopathological study of 34 cases

2010 ◽  
Vol 63 (8) ◽  
pp. 708-713 ◽  
Author(s):  
Matthew R Lindberg ◽  
Cyril Fisher ◽  
Khin Thway ◽  
Dengfeng Cao ◽  
John C Cheville ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Adverse Outcome ◽  
Soft Tissue Sarcomas ◽  
Mitotic Rate ◽  
Low Grade ◽  
Grading Systems ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
Degree Of Differentiation

BackgroundLeiomyosarcomas of the urinary bladder (LMS-UB) are rare, usually aggressive neoplasms. Owing to their rarity, only a limited number of cases with clinical follow-up information have been published. There is no current consensus on LMS-UB grading, and it is unknown whether the widely accepted Fédération Nationale des Centres de Lutte Contre le Cancer (FNCLCC) and National Cancer Institute (NCI) grading systems of soft-tissue sarcomas are applicable to LMS-UB.MethodsThe authors studied 34 well-characterised LMS-UB and compared the prognostic power of the FNCLCC and NCI systems with that of one published grading scheme for LMS-UB (Mayo). All available slides from 34 LMS-UB were retrieved and evaluated with regards to degree of differentiation, mitotic rate/10 high-powered fields (HPF), and % necrosis. Cases were graded using published criteria for the FNCLCC, NCI and Mayo schemes. Follow-up information was obtained.ResultsThe tumours occurred in 17 females and 17 males, ranging from 31 to 91 years (median 65), and measured 2–12 cm in size. One tumour was well differentiated, 17 tumours were moderately differentiated, and 16 tumours were poorly differentiated. Mitotic rates ranged from 1 to >30/10 HPF (median 12/10 HPF), and tumours showed 0–60% necrosis (median 25%). FNCLCC grades were 1 (3), 2 (12) and 3 (19). NCI grades were 1 (2), 2 (11) and 3 (21). Mayo grades were low (7) and high (27). FNCLCC and NCI grades were identical in 23/34 cases (68%). Four cases were FNCLCC/NCI grade 2 or 3 and Mayo low-grade. Clinical follow-up was available for 25 of 34 patients (74%). Clinical follow-up of ≥12 months was available for 17 of these 25 cases (68%) with a median follow-up duration of 52 months (range 12–120 months). Adverse outcome was seen in nine of these 17 patients (53%). Seven of the eight cases (88%) with a clinical follow-up duration of <12 months died of their disease. Overall, adverse outcome was documented in 16 of 25 (64%) cases. Metastatic disease was seen in 13 of 25 (52%) cases, with the lungs being the most common site of metastasis (62%). Adverse outcome was noted in 15 of 23 (65%) of FNCLCC grade 2 or 3 LMS-UB, as compared with zero of two (0%) FNCLCC grade 1 tumours (p=0.15), in 15 of 23 (65%) NCI grade 2 or 3 LMS-UB, versus zero of two (0%) NCI grade 1 sarcomas (p=0.17) and in 13 of 20 (65%) Mayo high grade LMS-UB, as opposed to two of five (40%) low-grade lesions (all results not statistically significant).ConclusionsThe authors conclude that LMS-UB occurs in older adults of either sex and is characterised by aggressive behaviour, with adverse outcome in >60% of cases. Certain advantages of the FNCLCC system may support its more widespread adoption for future studies.

Histopathological grading systems and their relationship with clinical parameters in lower lip squamous cell carcinoma

2014 ◽  
Vol 43 (5) ◽  
pp. 539-545 ◽  
Author(s):  
H.B. de P. Santos ◽  
A.L. do Ó Silva ◽  
L.H. de A. Cavalcante ◽  
P. M. Alves ◽  
G. P. Godoy ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Clinical Parameters ◽  
Lower Lip ◽  
Grading Systems ◽  
Histopathological Grading

scholarly journals TO ESTABLISH THE CORRELATION BETWEEN P53 AND HISTOPATHOLOGICAL GRADING OF GLIAL TUMORS

2021 ◽  
Vol 10 (4) ◽  
pp. 3421-3424
Author(s):  
Tabish Hassan
Keyword(s):  
Histopathological Examination ◽  
Histological Grade ◽  
Grading System ◽  
High Grade ◽  
Histological Grading ◽  
Biological Behaviour ◽  
P53 Expression ◽  
Glial Tumors ◽  
P53 Status ◽  
Histopathological Grading

Glial tumors occupy approximately 70% of the spectrum of all brain tumors with astrocytoma’s being the most common primary. High grade glial tumors have a poor outcome with limited survival rate. To establish the correlation between p53 status and histological grading of glial tumors. Objectives: To diagnose glial tumors on histopathological examination, to evaluate histological grade, to evaluate p53 expression and to assess the correlation between p53 expression and histological grade of gliomas. The study investigated 52 cases of gliomas. Histological grade was determined by WHO Grading System. Nuclear expression of p53 was evaluated by immunohistochemistry. A direct correlation between the histological grade and the p53 expression was observed. High grade gliomas exhibit high p53 expression. Thus, p53 as an adjunct to histological grade can provide a supportive clue to the clinicians, to predict the biological behaviour of gliomas.

scholarly journals Degree of differentiation of cutaneous squamous cell carcinoma: a comparison between a Swedish cohort of organ transplant recipients and immunocompetent patients

2018 ◽  
pp. 330-336 ◽  
Author(s):  
Carolina Stenman ◽  
Helena Gonzalez ◽  
Martin Gillstedt ◽  
Göran Dellgren ◽  
Bengt Hasséus ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Organ Transplant ◽  
Cutaneous Squamous Cell Carcinoma ◽  
University Hospital ◽  
Transplant Recipients ◽  
Organ Transplant Recipients ◽  
Poorly Differentiated ◽  
Degree Of Differentiation

Background: Organ transplant recipients (OTRs) have a very high risk of developing cutaneous squamous cell carcinoma (cSCC). Immunosuppressed OTRs may have a higher proportion of poorly differentiated cSCC than non-OTRs. Objectives: The aim of this study was to investigate the degree of differentiation of cSCCs in OTRs compared with immunocompetent individuals. Patients/Methods: Data from the Swedish Cancer Registry were crosschecked with data from the Transplant registry of the Transplant Institute at Sahlgrenska University Hospital in Gothenburg, Sweden. All OTRs with a diagnosis of cSCC, basosquamous carcinoma, and/or cSCC in situ established at the Department of Dermatology, Sahlgrenska University Hospital, during 2002-2015 were included. The control group consisted of non-OTRs with the same diagnoses during the same time period. Results: During 2002-2015, 82 OTRs diagnosed with 515 tumors and 883 non-OTRs with 1,247 tumorswere included. OTRs developed 0.47 tumors/year vs 0.10 tumors/year for non-OTRs, but no significant differences were observed in the degree of tumor differentiation of invasive cSCCs between OTRs and non-OTRs (P = 0.4). The distribution of poorly, moderately, and well-differentiated invasive cSCCs among OTRs and non-OTRs were 8.5% vs 12.5%, 22.1% vs 29.9%, and 69.4% vs 57.6%, respectively. Conclusions: OTRs do not develop a higher proportion of poorly differentiated cSCCs than non-OTRs.

scholarly journals Role of p53 expression in the diagnosis of oral squamous cell carcinoma

2019 ◽  
Vol 10 ◽  
Author(s):  
Mara Falla ◽  
Andrea Gabusi ◽  
Achille Tarsitano ◽  
Roberto Rossi ◽  
Chiara Amadasi ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
P53 Expression

scholarly journals Corellation of p53 expressions and histopathological grading in oral cavity squamous cell carcinoma

2016 ◽  
Vol 49 (3) ◽  
pp. 120
Author(s):  
Silvi Kintawati
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cavity ◽  
Cell Carcinoma ◽  
Cancer Progression ◽  
Squamous Cell ◽  
P53 Mutation ◽  
Clinical Staging ◽  
P53 Expression ◽  
P53 Antibodies ◽  
Histopathological Grading

Background: Squamous cell carcinoma is a malignancy of oral cavity mostly occurred and can also metastasize. p53 gene is a tumor suppressor gene that plays an important role in carcinogenesis. The role of wild-type p53 is very important in suppressing the formation of a malignancy. p53 also has many other important functions. p53 is a suppressor of tumor/ cancer progression through the response of cell cycle to DNA damage and by giving time to repair DNA prior to replication of genes. p53 mutation, mostly occurs in a malignancy, so earlier histopathological transformation can be detected by observing p53 mutation. The prognosis of squamous cell carcinoma in oral cavity, therefore, depends on histopathological grading and clinical staging of the tumor. To enforce the histopathological grading, in addition based on histopathology differentiation, the earlier histopathological transformation can also be assessed. Purpose: This study aimed to determine the correlation of p53 expressions and histopathological grading in oral cavity squamous cell carcinoma. Method: This study was a retrospective study on 20 cases of oral cavity squamous cell carcinoma examined at Department of Pathology Anatomy in Hasan Sadikin Hospital in Bandung. Immunohistochemical examination was then performed using p53 antibodies to determine the correlation of p53 expression and histopathological grading in oral cavity squamous cell carcinoma to predict prognosis. Result: The overall results showed that there was no correlation between p53 expression and histopathological grading in oral cavity squamous cell carcinoma of the oral cavity although there was a very strong correlation between p53 expression and histopathological grading I (p<0.01). Conclusion: It can be concluded that there was no correlation between p53 expression and histopathological grading in oral cavity squamous cell carcinoma. Thus, p53 expression cannot be used to predict a prognosis.

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