scholarly journals Malignant Melanocytic Neural Crest Tumor in a Young Chicken (Gallus domesticus)

1995 ◽  
Vol 32 (5) ◽  
pp. 535-537 ◽  
Author(s):  
S. Hafner ◽  
K. Latimer ◽  
L. C. Kelley ◽  
K. Wortham ◽  
M. Puette
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ganglion Cells ◽  
Neuron Specific Enolase ◽  
Gallus Domesticus ◽  
Specific Staining ◽  
Melanocytic Tumor ◽  
Transmission Electron ◽  
S 100 ◽  
Young Chicken

A malignant melanocytic tumor was found in an 8-week-old chicken. The tumor, which was composed of melanocytes, ganglion cells, nerves, and primitive pressure receptors, was examined by light microscopy, transmission electron microscopy, and immunohistochemistry. Antibodies to neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP), and S-100 protein variably stained nerves, but melanocytes were only rarely immunolabelled by NSE antibodies and there was no specific staining of these cells for S-100 or GFAP. Ultrastructurally, neoplastic melanocytes contained melanin within melanosomes and premelanosomes and did not resemble Schwann cells.

Functional morphology of lenticular accommodation in the young chicken (Gallus domesticus)

1991 ◽  
Vol 69 (8) ◽  
pp. 2183-2193 ◽  
Author(s):  
J. A. West ◽  
J. G. Sivak ◽  
M. J. Doughty
Keyword(s):  
Electron Microscopy ◽  
Sarcomere Length ◽  
Muscle Fibers ◽  
Gallus Domesticus ◽  
Ciliary Muscle ◽  
Eye Muscle ◽  
Ciliary Process ◽  
Transmission Electron ◽  
Ciliary Processes ◽  
Young Chicken

The morphology of the accommodative apparatus in chicks has not been fully characterized, and the accommodative mechanism is not well understood. A detailed study of the ciliary muscle and ciliary process morphology in newly hatched and 2-week-old chicks has been carried out in this investigation. The methods include light microscopy and scanning and transmission electron microscopy. Accommodation is induced with nicotine sulphate in young chicks to compare the morphology of the accommodative apparatus in a relaxed and accommodated state. This study confirms the striated nature of the ciliary muscle in the developing chick eye and the direct articulation of the ciliary processes with the annular pad of the lens. The muscle fibers of the nicotine-treated eyes are shorter than those of the control eyes and appear contracted. Quantitatively, the fibers of the nicotine-treated eyes have a significantly shorter sarcomere length than control eye muscle fibers. The ciliary folds of the nicotine-treated eyes are very convoluted and are significantly shorter in length than control eye folds. Evidence from this investigation suggests that the contraction of the ciliary muscle causes the ciliary folds to exert direct force onto the annular pad of the lens during accommodation.

Orbital (Retrobulbar) Meningioma in a Simmental Cow

2007 ◽  
Vol 44 (4) ◽  
pp. 504-507 ◽  
Author(s):  
J. L. Reis ◽  
C. T. Kanamura ◽  
G. M. Machado ◽  
R. O. França ◽  
J. R. J. Borges ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Epithelial Membrane Antigen ◽  
Smooth Muscle Actin ◽  
Neuron Specific Enolase ◽  
Muscle Actin ◽  
Transmission Electron ◽  
S 100 ◽  
Poorly Differentiated ◽  
The Right ◽  
Mib 1

A 12-year-old Simmental cow was presented with a moderately firm irregular whitish mass of approximately 5 cm in diameter, occupying the right orbit. Microscopically, a poorly differentiated neoplasm was observed. The immunohistochemical panel included cytokeratins, vimentin, epithelial membrane antigen, Factor VIII, CD34, Mart-1, Melan A, smooth muscle actin, desmin, chromogranin, neuron-specific enolase, S-100 protein, and MIB-1. The neoplasm was negative for all of them, with the exception of vimentin and S-100 protein. Transmission electron microscopy revealed abundant desmosomes. These findings support the diagnosis of orbital (retrobulbar) meningioma.

Psammomatous duodenal carcinoid producing somatostatin: Ultrastructural investigation

1987 ◽  
Vol 45 ◽  
pp. 850-851
Author(s):  
G. Ilse ◽  
K. Kovacs ◽  
G. Gardiner ◽  
T. Moore
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Neuron Specific Enolase ◽  
Psammoma Bodies ◽  
Biliary Pain ◽  
Alizarin Red ◽  
Α Subunit ◽  
X Ray ◽  
Transmission Electron ◽  
Duodenal Carcinoid

Somatostatin producing duodenal psammomatous carcinoids, described first by Kaneko et al. in 1979, are rare. We have studied such a tumor by histology, immunohistochemistry, transmission electron microscopy, immunoelectron microscopy and energy dispersive x-ray microanalysis.A 60-year-old woman with attacks of biliary pain underwent cholecystectomy. At surgery, a tumor measuring 3 cm in diameter was discovered in the wall of the duodenum. Transduodenal biopsy revealed a psammomatous carcinoid exhibiting strong immunopositivity for somatostatin. The tumor was removed 4 months later. By histology, the tumor showed a tubular pattern as well as a cribriform arrangement, contained amorphous hematoxylinophil deposits and psammoma bodies which were positive with the von Kossa method and alizarin red. The avidin-biotin-peroxidase complex technique demonstrated positivity for somatostatin, mAB lu-5 (a monoclonal panepithelial antibody), keratin, neuron specific enolase and chromogranin. No immunoreactivity was obtained for ACTH, calcitonin, endorphin, FSH, gastrin, glucagon, GH, insulin, LH, neurophysin, PP, PRL, serotonin, α-subunit, VIP and vasopressin.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

Direct Observation of Heat Exchanger Deposits by Cross Sectioning

1967 ◽  
Vol 25 ◽  
pp. 364-365
Author(s):  
R. W. Anderson ◽  
D. L. Senecal
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Heat Exchanger ◽  
Direct Observation ◽  
Cross Sections ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Flowing Water ◽  
Transmission Electron ◽  
Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

Phase Transformations in Ti-7w/oMo-3w/oMe Alloy

1974 ◽  
Vol 32 ◽  
pp. 514-515
Author(s):  
S. Fujishiro
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Transmission Electron Microscopy ◽  
Tensile Strength ◽  
Mechanical Processing ◽  
Ray Method ◽  
X Ray ◽  
Transmission Electron ◽  
Water Quench ◽  
Alpha Beta

The mechanical properties of three titanium alloys (Ti-7Mo-3Al, Ti-7Mo- 3Cu and Ti-7Mo-3Ta) were evaluated as function of: 1) Solutionizing in the beta field and aging, 2) Thermal Mechanical Processing in the beta field and aging, 3) Solutionizing in the alpha + beta field and aging. The samples were isothermally aged in the temperature range 300° to 700*C for 4 to 24 hours, followed by a water quench. Transmission electron microscopy and X-ray method were used to identify the phase formed. All three alloys solutionized at 1050°C (beta field) transformed to martensitic alpha (alpha prime) upon being water quenched. Despite this heavily strained alpha prime, which is characterized by microtwins the tensile strength of the as-quenched alloys is relatively low and the elongation is as high as 30%.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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