Panton-Valentine Leukocidin Gene Detected in a Staphylococcus Aureus Strain Isolated from a Knee Arthroprosthesis Infection

2009 ◽  
Vol 32 (9) ◽  
pp. 630-634 ◽  
Author(s):  
Lucio Montanaro ◽  
Lucilla Baldassarri ◽  
Tolmino Corazzari ◽  
Roberta Creti ◽  
Stefano Ravaioli ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Research Unit ◽  
Aureus Strain ◽  
Toxin Gene ◽  
Strain Collection ◽  
Rizzoli Orthopaedic Institute ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

This report focuses on the molecular characterization of a Staphylococcus aureus strain isolated from a knee arthroprosthesis infection and recognized retrospectively as a carrier of the Panton-Valentine leukocidin gene. The stored microbiological isolate, which belonged to the strain collection of the Research Unit on Implant Infections of the Rizzoli Orthopaedic Institute, was retrieved for molecular analysis. Genotyping was carried out, revealing an interesting profile. In addition to the positivity for the Panton-Valentine toxin gene, the results indicated that the isolate belonged to the agr III group and was endowed with bbp and cna genes, both encoding for staphylococcal adhesins that bind bone proteins. The strain had the mecA gene for methicillin resistance, even though it was unable to resist any of the β-lactam or other antibiotics. Its gene configuration matched that of other community-acquired methicillin-resistant and methicillin-susceptible Staphylococcus aureus (CA-MRSA and CA-MSSA) strains which have recently been reported worldwide. As far as we know, this is the first report on a PVL-positive S. aureus strain associated with an orthopedic implant (knee arthroprosthesis) infection.

scholarly journals Staphylococcus aureus carrying lukS/F Panton-Valentine Leukocidin (PVL) toxin genes in hospitals of Lahore city

2018 ◽  
Vol 12 (09) ◽  
pp. 720-725
Author(s):  
Muhammad Shaheen Iqbal ◽  
Yasar Saleem ◽  
Farheen Ansari ◽  
Muhammad Usman Qamar ◽  
Sania Mazhar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care ◽  
Meca Gene ◽  
Toxin Gene ◽  
Mannitol Salt Agar ◽  
Burn Wounds ◽  
High Level ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Introduction: Panton Valentine-Leukocidin (PVL) toxin is secreted by Staphylococcus aureus and is mostly associated with skin and soft tissue infections (SSTI). This study aims to find out the prevalence of lukS/F-PV gene, which encode PVL toxin from strains of SSTI, burn wounds and nasal colonizers of out-patients and to measure the antimicrobial susceptibility of S. aureus isolates. Methodology: This is an analytical observational cross-section study and was conducted from July 2014 to June 2015 at four tertiary care hospitals and PCSIR Laboratories Complex, Lahore, Pakistan. A total of 376 random clinical swabs were collected from SSTI (n = 179), nasal nares (n = 134) and burn wounds (n = 63) from out-patients’ departments (OPD). The specimens were cultured on nutrient and mannitol salt agar (MSA) and the organism was identified by catalase, coagulase, and DNase tests. Antimicrobial susceptibility, methicillin, inducible clindamycin, and high-level mupirocin (HLMR) resistance were determined as per CLSI guidelines. Molecular identification of mecA and lukS/F-PV genes was performed by PCR. Results: We isolated 127 S. aureus, where 41 (32.3%) were MRSA and 86 (67.7%) were MSSA. All MRSA carried mecA gene whereas lukS/F-PV gene was found in 21 MRSA and 31 MSSA strains. Overall, a high antimicrobial resistance was found against MRSA and lukS/F-PV positive MSSA. Inducible clindamycin and high-level mupirocin resistance (HLMR) was 23.6% and 19.5% respectively. Conclusions: A high rate of PVL toxin gene was detected among S. aureus strains and a high prevalence of antimicrobial resistant strains was observed.

scholarly journals The Role of Staphylococcus aureus in Mastitis

2019 ◽  
Vol 36 (3) ◽  
pp. 503-509 ◽  
Author(s):  
Sara Giordana Rimoldi ◽  
Paola Pileri ◽  
Martina Ilaria Mazzocco ◽  
Francesca Romeri ◽  
Giovanna Bestetti ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Median Number ◽  
Clinical Severity ◽  
Toxin Gene ◽  
Lactation Period ◽  
Breast Abscess ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Background Breastfeeding women are at risk of developing mastitis during the lactation period. Staphylococcus aureus has emerged as the community-acquired pathogen responsible for virulence (methicillin resistance and Panton-Valentine leukocidin toxin producing). Research aim The aim was to compare the microorganisms responsible for mastitis and breast abscesses during breastfeeding. Methods This observational study was conducted with a sample of women ( N = 60) admitted to our hospital between 2016 and 2018. Participants affected by mastitis and breast abscess were studied and cared for by a multidisciplinary working group. A diagnostic breast ultrasound identified the pathology. Results Twenty-six participants (43.3%) were affected by mastitis and 34 (56.7%) by breast abscess. The most common microorganism identified was Staphylococcus aureus ( S. aureus; mastitis, n = 13; abscesses, n = 24). Methicillin resistance was identified in 21 (44.7%) S. aureus strains: 17 (80.9%) cases of abscess and four (19.1%) cases of mastitis. The median number of months of breastfeeding was smaller in the methicillin-resistant S. aureus (MRSA) cases (median = 3, range = 1–20 months) than in the methicillin-sensitive S. aureus (MSSA) cases (median = 6.5, range = 3–21 months). The Panton-Valentine leukocidin toxin gene was detected in 12 (25.5%) cases (MRSA, n = 8, 66.7%; MSSA, n = 4, 33.3%). Hospitalization was required more frequently in MRSA ( n = 8, 38%; five Panton-Valentine leukocidin positive) than in MSSA cases ( n = 5, 19%; one Panton-Valentine leukocidin positive). Four women out of the eight MRSA cases (50%) that were Panton-Valentine leukocidin positive stopped breastfeeding during mammary pathologies, three (37.5%) participants continued breastfeeding until the follow-up recall, and one case was lost at follow-up. Conclusion Clinical severity was probably complicated by the presence of the Panton-Valentine leukocidin toxin, which required hospitalization more frequently.

scholarly journals Panton-Valentine Leukocidin-Producing Staphylococcus aureus Infection: A Case Series

2020 ◽  
Vol 12 (3) ◽  
pp. 61-69
Author(s):  
Beatriz Prista-Leão ◽  
Isabel Abreu ◽  
Raquel Duro ◽  
André Silva-Pinto ◽  
Filipa Ceia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Case Series ◽  
Adult Males ◽  
Gene Search ◽  
Physician Awareness ◽  
Travel Abroad ◽  
Adequate Management ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Panton-Valentine leukocidin-producing Staphylococcus aureus (PVL-SA) is associated with relapsing multifocal skin and soft tissue infections (SSTI), necrotizing pneumonia (NP) and severe musculoskeletal infections. Epidemiology is underknown and underdiagnosis is likely. Recent travel abroad, case clustering and relapsing disease are often reported. We reviewed all cases of PVL-SA infection diagnosed at our center, and found 21 cases over a 43-month period. Most patients were adult males, had relevant travel history, reported recurrent disease and presented with SSTI. Etiologic diagnosis took up to five years; meanwhile, 42% of patients had antibiotic treatments. Draining procedures were required in 43% of patients and intensive care support in 19%. All patients recovered. Methicillin-resistance prevalence was 24%. Only 2/13 decolonized patients had posterior relapsing SSTI, both with likely infected contacts. PVL-SA infection’s severity and impact are clear, even in small case series as ours. Physician awareness and active PVL-gene search are crucial for an adequate management.

scholarly journals Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje

2016 ◽  
Vol 73 (3) ◽  
pp. 256-260 ◽  
Author(s):  
Ljiljana Petrovic-Jeremic ◽  
Nada Kuljic-Kapulica ◽  
Elizabeta Ristanovic ◽  
Dragana Josic ◽  
Zorica Lepsanovic
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clinical Laboratory ◽  
Meca Gene ◽  
Latex Agglutination ◽  
The Other ◽  
Methicillin Resistant ◽  
Standard Polymerase Chain Reaction ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Background/Aim. Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains appear to have rapidly disseminated among population in the community without established risk factors for MRSA worldwide. Panton-Valentine leukocidin (PVL) is a cytolytic toxin, encoded by the lukF-PV and lukF-PV genes. PVL may be the key toxin responsible for enhanced virulence of CA-MRSA. The aim of this study was to detect the genes encoding PVL in CA-MRSA isolates from healthy people from the District of Pomoravlje. Methods. We took throat and nose swabs from healthy, employed persons with mandatory sanitary examinations and analyzed the presence of MRSA, between January 2011 and December 2012 in the District of Pomoravlje. Susceptibility of isolated strains to cefoxitin was investigated by using disc diffusion according to the recommendation of CLSI (Clinical Laboratory Standard Institute), and by E test. The presence of penicillin-binding protein 2a (PBP2a) in Staphylococci was detected using latex agglutination Slidex ?MRSA Detection test. The gold standard, polymerase chain reaction (PCR) assay, was used for detection of mecA gene and PVL gene, and typing of SCCmec region. Results. Our investigation showed that staphylococcal carrier state was present in 2.58% of 52,910 throat and nasal swabs, and in 50 of them (3.67%) MRSA was isolated. Among these MRSA, 2 (4%) isolates were PVL-positive. Conclusion. The prevalence of CAMRSA and the presence of PVL gene among healthy, employed population in the District of Pomoravlje were low. The values obtained in this study show that, our region is not significantly different from the other parts of our country, nor from the other European countries.

scholarly journals Inhibition of Staphylococcus aureus LC 554891 by Moringa oleifera Seed Extract either Singly or in Combination with Antibiotics

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4583
Author(s):  
Gamal Enan ◽  
Abdul-Raouf Al-Mohammadi ◽  
Samir Mahgoub ◽  
Seham Abdel-Shafi ◽  
Eman Askar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oils ◽  
Inhibitory Activity ◽  
Moringa Oleifera ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Resistance Index ◽  
Aureus Strain ◽  
Rrna Gene ◽  
Bacterial Strains

Bacterial outbreaks caused by Staphylococcus aureus (S. aureus) are interesting due to the existence of multidrug resistant (MDR) isolates. Therefore, there is a need to develop novel ways to control such MDR S. aureus. In this study, some natural agents such as honey bee (HB), extracts of either Moringa oleifera seeds (MSE), or leaves (MLE) and essential oils of garlic, clove, and moringa were studied for their inhibitory activity against this S. aureus pathogen. About 100 food samples including beef luncheon (n = 25), potato chips (n = 50), and corn flakes (n = 25) were investigated for possible pollution with the S. aureus bacteria. The isolated bacteria suspected to belong S. aureus that grew well onto Baird–Parker agar (Oxoid) and shiny halo zones and positive coagulase reaction were selected and identified by API-Kits; all of them that were approved belong to S. aureus (18 strains). The sensitivity of the obtained 18 S. aureus bacterial strains to 12 antibiotics were evaluated; all of them were resistant to ofloxacin; however, other antibiotics tested showed variable results. Interestingly, the S. aureus No. B3 isolated from beef luncheon was resistant to 10 antibiotics out of 12 ones tested. Multiple antibiotic resistance index (MAR) of this S. aureus strain was about 83.3%. Therefore, its identification was confirmed by sequencing of a 16S rRNA gene which approved a successful biochemical identification carried out by API Kits and such strain was designated S. aureus LC 554891. The genome of such strain appeared to contain mecA gene encoding methicillin resistance; it was found to contain hla, hlb, tsst-1, and finbA that encode α-blood hemolysis, β-blood hemolysis, toxic shock syndrome gene, and fibrinogen-binding protein gene, respectively. In addition, the virulence factors viz. sea; seb; sec encoding enterotoxins were detected in the DNA extracted from S. aureus B3 strain. Aqueous extract of Moringa oleifera seeds (MSE) showed inhibitory activity against S. aureus LC 554891 better than that obtained by tetracycline, essential oils or HB. Minimum inhibitory concentration (MIC) of MSE was 20µg/mL. Instrumental analysis of MSE showed 14 bioactive chemical compounds. Combinations of both MSE and tetracycline showed distinctive inhibitory activity against S. aureus LC 554891 than that obtained by either tetracycline or MSE singly.

#82: Osteomyelitis with Panton-Valentine Leukocidin Producing Staphylococcus aureus Strains, Experience in a Tertiary Pediatric Hospital in Mexico City

2021 ◽  
Vol 10 (Supplement_1) ◽  
pp. S9-S9
Author(s):  
Nancy Aguilar ◽  
Alejandra Aquino ◽  
Guillermo Vázquez ◽  
Jocelin Mérida ◽  
Oscar Isunza-Alonso ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mexico City ◽  
Clinical Features ◽  
Methicillin Resistance ◽  
Tertiary Hospital ◽  
Antimicrobial Treatment ◽  
High Rate ◽  
Critical Event ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Abstract Background S. aureus is the most common cause of osteomyelitis in children. Panton-Valentine leucocidin (PVL) is an exotoxin produced by certain S. aureus strains, which can be detected in both methicillin-sensitive and -resistant strains of staphylococci. Pediatric osteomyelitis by PVL producing S. aureus constitute a rare, but highly critical event. They are characterized by a rapid course of marked inflammation, worsening under conservative therapy, and a high rate of recurrence. No information is available on osteomyelitis and Panton-Valentine leukocidin producing S. aureus in Mexico. Objective The aim of this study was to determine the molecular characterization of S. aureus strains isolated in pediatric patients with osteomyelitis and their clinical features in a tertiary hospital in Mexico City. Methods We conducted a prospective study of children admitted for osteomyelitis, between December 2018 and November 2019, at Instituto Nacional de Pediatría. We obtained an informed consent in children under 12 years of age and an informed assent in children over 12. The confirmation of the S. aureus isolates was performed by amplification and analysis of 16S rRNA and nuc genes. The mecA and pvl genes were detected by PCR, the clinical features were obtained at the admission. Results Fifty patients were included and 26 cases of osteomyelitis were diagnosed, 13 were due to staphylococcus aureus genus and 6 due to S. aureus; all of them were methicillin susceptible and two were PVL producers. Both patients had a severe initial presentation, with extensive local abscesses, and one required three surgical procedures and developed septic pulmonary embolism. The two patients received double antimicrobial treatment and required a long therapy interval. Conclusions Osteomyelitis with Panton-Valentine Leukocidin producing S. aureus seems to be more severe. In patients with severe osteomyelitis, it is essential to detect the PVL toxin because they require early surgical intervention and prolonged intravenous therapy. Our findings suggest that the severity of the osteomyelitis is linked with PVL production more than with methicillin resistance due to that all our isolates were methicillin susceptible.

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