Biochemical Changes following Parathyroidectomy

1993 ◽  
Vol 16 (10) ◽  
pp. 700-703 ◽  
Author(s):  
P.K.G. Chandran ◽  
T.J. Ulahannan ◽  
M. Skiles
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Calcium ◽  
Serum Alkaline Phosphatase ◽  
Inorganic Phosphorus ◽  
Biochemical Changes ◽  
Postoperative Hypocalcemia ◽  
Subtotal Parathyroidectomy ◽  
Osteoblastic Activity ◽  
End Stage ◽  
Phosphorus Levels

Biochemical changes that had appeared after subtotal parathyroidectomy (PTx) in 26 patients with end-stage renal failure were observed. The volume of excised parathyroid glands was also measured. Serum calcium and inorganic phosphorus levels fell after PTx; only to rise in due course. Serum alkaline phosphatase levels rose after PTx, reaching a peak by the 14th postoperative day. These elevated levels returned to normal range at about three months after PTx. Strong correlation was noted among the degree of postoperative hypocalcemia, and increase in serum alkaline phosphatase, but not between absolute pre or postoperative alkaline phosphatase levels and changes in serum calcium or phosphorus concentrations. Nevertheless, significant correlation was seen between pre-PTx levels of alkaline phosphatase and its short-lived postoperative rise, indicating hastened osteoblastic activity.

Immunoradiometric method and electrophoretic system compared for quantifying bone alkaline phosphatase in serum

1995 ◽  
Vol 41 (6) ◽  
pp. 853-857 ◽  
Author(s):  
V O Van Hoof ◽  
M Martin ◽  
P Blockx ◽  
A Prove ◽  
A Van Oosterom ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Malignant Disease ◽  
Screening Method ◽  
Bone Alkaline Phosphatase ◽  
Cross Reactivity ◽  
Osteoblastic Activity ◽  
Alp Activity ◽  
Agarose Electrophoresis ◽  
End Stage ◽  
Rule Out

Abstract Agarose electrophoresis (Isopal, Beckman) and an immunoradiometric assay (IRMA) involving specific monoclonal antibodies (Ostase, Hybritech), two methods for the quantification of serum bone alkaline phosphatase (ALP, EC 3.1.3.1), a marker of osteoblastic activity, were compared in 293 patients: 79 with end-stage renal failure treated with hemodialysis and 214 with malignant disease. Overall correlation between the two methods was good (r = 0.92), except (a) for low values of bone ALP and (b) in some samples with high total liver ALP activity--both due to considerable cross-reactivity of the anti-bone ALP antibodies of the Ostase kit with liver ALP. This interference was not constant and was not evenly distributed across all concentrations of bone ALP. Low bone ALP determined with the IRMA (< or = 5 micrograms/L) was confirmed by electrophoresis (< or = 21 U/L), but bone ALP activity determined by electrophoresis to be low (< or = 21 U/L) was not correlated with the IRMA results. After standardizing our results by computing z-values for bone ALP, delta z (= zOstase - zIsopal) was significantly correlated with liver ALP activity (r = 0.73, P < 0.0001). We conclude that the IRMA for quantifying bone ALP is acceptable as a screening method. However, when high values for bone ALP are found with the Ostase method, confirmation by electrophoresis remains mandatory to rule out cross-reactivity with high amounts of liver ALP. For detecting low bone ALP activities, electrophoresis remains the method of choice.

BIOASSAY OF PARATHYROID HORMONE IN RATS BY DETERMINATION OF PLASMA CALCIUM, URINARY 32P EXCRETION AND SERUM ALKALINE PHOSPHATASE

1966 ◽  
Vol 35 (3) ◽  
pp. 229-238 ◽  
Author(s):  
R. J. TREACHER
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Serum Calcium ◽  
Calcium Concentration ◽  
Serum Alkaline Phosphatase ◽  
Plasma Calcium ◽  
Significant Alteration ◽  
Good Precision ◽  
Serum Calcium Concentration

SUMMARY Methods for assay of parathyroid hormone based on an increase in serum calcium concentration, urinary 32P excretion and serum alkaline phosphatase elevation in parathyroidectomized rats have been compared and modifications introduced to improve sensitivity, precision, speed and ease of manipulation. Both the serum calcium and urinary 32P assay gave good precision (mean λ = 0·23 and 0·29, respectively) but by the serum calcium method less than 10 USP units of parathyroid hormone could not be detected, whereas the phosphaturic assay detects as little as 0·5 USP unit. Both assays are simple to perform and each requires only 2 days to complete. They can be combined in a single design using the same animals. Assays based on serum alkaline phosphatase levels in parathyroidectomized rats were not successful since it was impossible to produce a significant alteration in serum alkaline phosphatase by the administration of parathyroid hormone.

scholarly journals Bone Density, Osteocalcin and Deoxypyridinoline for Early Detection of Osteoporosis in Obese Children

2015 ◽  
Vol 3 (3) ◽  
pp. 413-419 ◽  
Author(s):  
Ghada El-Dorry ◽  
Hala Ashry ◽  
Tarek Ibrahim ◽  
Tahany Elias ◽  
Fatma Alzaree
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Density ◽  
Serum Calcium ◽  
Negative Correlation ◽  
Total Mass ◽  
Serum Alkaline Phosphatase ◽  
Significant Negative Correlation ◽  
Whole Body ◽  
Serum Osteocalcin ◽  
Obese Children

AIM: This study aimed at comparing between bone density using DEXA, serum osteocalcin and urinary DPD in obese and non obese prepubertal children. METHODS: After taking the consent of eighty children they were subjected to: full examination, anthropometric measurements, blood samples were withdrawn to determine serum osteocalcin, Ca, Ph, alkaline phosphatase, and urinary DPD. Bone densities, body composition of the whole body were examined using DEXA. Data were analyzed using SPSS.RESULTS: All anthropometric variables showed significant increase in obese children except for height in comparison to control group. Total mass, lean + BMC, lean, fat, area, BMC, BMD and Z score of the whole body were significantly increased in obese children. Serum calcium showed significant increase while alkaline phosphatase was significantly decreased in obese children. DPD showed no significant difference between obese and non obese children. Significant negative correlation was found between ca, lean, lean + BMC and total mass. Serum alkaline phosphatase showed also a significant negative correlation with (lean + BMC and total mass). Serum osteocalcin showed negative significant correlation with area, BMC, BMD, lean and Z score.CONCLUSION: Obese children showed significant increase in anthropometric and DEXA parameters, increase in serum calcium and significant decrease in serum alkaline phosphatase. Osteocalcin was negatively correlated with most of DEXA results.

scholarly journals Variations in serum calcium, inorganic phosphorus and magnesium levels due to stage of lactation, season, and age in Ayrshire cows injected with vitamin D3 prior to calving

1970 ◽  
Vol 42 (1) ◽  
pp. 8-20
Author(s):  
Vappu Kossila ◽  
Paavo Niemelä ◽  
Erkki Koskenkorva
Keyword(s):  
Vitamin D ◽  
Serum Calcium ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Inorganic Phosphorus ◽  
Feeding Period ◽  
Phosphorus Level ◽  
Blood Samples ◽  
Grazing Period ◽  
Phosphorus Levels

Blood mineral studies were carried out in the dairy herd of the Viik Experimental Farm, in which parturient paresis has been successfully prevented by intramuscular injections of vitamin D3 (2 X 5 million I.U.). The material consisted of 41 cows from which 207 blood samples had been taken in 1963 and 12 cows (6 controls, 6 vitamin D injected cows) from which 72 blood samples had been taken in 1969. All cows were either dry and pregnant or lactating and nonpregnant. Compared to values obtained during the dry period, the serum calcium level was only slightly lower shortly after calving. The calcium level increased in the vitamin D treated cows during the ascending phase of lactation, whereas both the calcium and inorganic phosphorus levels remained relatively low in the control cows, respectively. The highest mean magnesium levels occurred during the 2nd and 3rd weeks of lactation. The serum calcium level was higher during the grazing period than during the indoor feeding period. Inorganic phosphorus remained at a relatively high level in younger cows during the grazing period, whereas a significant decrease was noted in the phosphorus level of older cows, respectively. The lowest magnesium values were noted in May, i.e. already before the beginning of the pasture season, the concentration increasing towards the end of the grazing period especially in younger cows. As a whole, younger cows had slightly higher mean serum calcium and magnesium levels and significantly higher inorganic phosphorus levels compared to older cows. The effect of age on the inorg. phosphorus level appeared to be more marked in dry cows and during the grazing period than in lactating cows and during the winter feeding period. Special attention has been paid to the blood mineral values of four individual cows suffering from various illnesses during the study period.

Comparison between CEA, TPA, CA 15/3 and Hydroxyproline, Alkaline Phosphatase, Whole Body Retention of 99mTc MDP in the follow-up of Bone Metastases in Breast Cancer

1990 ◽  
Vol 5 (2) ◽  
pp. 65-72 ◽  
Author(s):  
G. Francini ◽  
M. Montagnani ◽  
R. Petrioli ◽  
P. Paffetti ◽  
S. Marsili ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Alkaline Phosphatase ◽  
Bone Metastases ◽  
Serum Alkaline Phosphatase ◽  
Whole Body ◽  
Lower Sensitivity ◽  
Osteoblastic Activity ◽  
Body Retention ◽  
Hydroxyproline Excretion

The development of bone metastases in cancer can be monitored easily using three markers: 24 h urinary hydroxyproline excretion (HOP) (an index of osteoclastic activity), serum alkaline phosphatase (Alk.Ph.) (an index of osteoblastic activity) and 24 h whole body retention of 99mTc-methylene diphosphonate (WBR%) (an index of bone turnover). To evaluate the effectiveness of this group of bone tumor markers in breast cancer we compared it with the following group of three markers which are commonly used in the monitoring of breast cancer and in the follow-up of advanced disease with or without bone metastases: carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA) and breast carcinoma antigen (CA 15/3). In 48 patients with bone metastases CEA, TPA and CA 15/3 were shown to be sensitive (79%, 85%, 90% respectively), while HOP, Alk.Ph. and WBR%, which are commonly accepted as reliable markers of bone activity, showed a lower sensitivity (67%, 46%, 75% respectively). These results may be explained by the lack of osteoclastic or osteoblastic (or both) activity at the time of diagnosis. This explanation is supported by the fact that the bone markers HOP, Alk.Ph. and WBR% were found to be more sensitive than the others in the subsequent follow-up study. We conclude that in our study, CEA, TPA and CA 15/3 are at first more sensitive than Alk. Ph., HOP and WBR% but during the follow-up Alk.Ph., HOP and WBR% are possibly both more specific and more sensitive

Rickets Associated With Long-Term Anticonvulsant Therapy

PEDIATRICS ◽  
1977 ◽  
Vol 60 (2) ◽  
pp. 261-262
Author(s):  
Francesca Severi ◽  
Maria Clara Gasparoni ◽  
Carla Martini
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Anticonvulsant Therapy ◽  
Control Population ◽  
Calcium And Phosphorus ◽  
Phosphorus Levels ◽  
At Home

The study presented by Crosley et al. in the July 1975 issue of Pediatrics (page 52) is the first dealing with noninstitutionalized children. A significantly higher value of serum alkaline phosphatase was observed in treated children than in the control population, while calcium and phosphorus abnormalities were minimal in both groups. We concluded a study on the serum alkaline phosphatase, calcium, and phosphorus levels in noninstitutionalized children, including 50 who were from 3 to 14 years old and who were receiving long-term anticonvulsant therapy (either diphenylhydantoin, phenobarbital, or both for more than 12 months) and 57 age-matched controls, all living at home.

Changes in Serum Calcium, Albumin and Alkaline Phosphatase Levels of Postmenopausal Women

2019 ◽  
Vol 8 (2) ◽  
pp. 30-35
Author(s):  
Kamana Rani Saha ◽  
Susmita Das ◽  
Shahidul Islam ◽  
AHM Hasan ◽  
Rafika Afrose ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Postmenopausal Women ◽  
Serum Calcium ◽  
Serum Alkaline Phosphatase ◽  
Colorimetric Method ◽  
Medical College Hospital ◽  
Reproductive Age ◽  
College Hospital ◽  
Medical College ◽  
Increased Risk

A case-control cross-sectional study was carried out to observe the changes in serum calcium, albumin and alkaline phosphatase levels of postmenopausal women compared to their premenopausal counterparts. This is to enable us ascertain the relative risk of developing osteoporosis in postmenopausal women in Bangladesh. One hundred and eighteen (118) apparently healthy females (59 premenopausal=control and 59 postmenopausal=case) were selected for the study. The study was carried out in the Department of Biochemistry, Mymensingh Medical College in cooperation with the Department of Gynecology, Mymensingh Medical College Hospital and Community Based Medical College Hospital Bangladesh during the period from January to December 2009. Data were collected through clinical evaluation from pre-selected questionnaires. Fasting venous blood samples were taken for laboratory investigations. Serum calcium and albumin was determined using colorimetric method and enzymatic end point method was used to determine serum alkaline phosphatase levels. Statistical significance of difference between two groups were evaluated by using Student's unpaired 't' test with the help of SPSS software package. The results showed statistically significant decrease in serum calcium and albumin (p<0.01) of menopausal women compared to reproductive age group. The postmenopausal women had significantly higher (p<0.01) concentrations of serum alkaline phosphatase than the premenopausal women with regular menstruation. Therefore, it can be concluded that menopause leads to the increased risk of osteoporosis by decreasing serum calcium and albumin and by increasing alkaline phosphatase levels. The decreased serum calcium and increased alkaline phosphatase levels indicate that menopause is an independent risk factor for developing osteoporosis in women of Bangladesh. CBMJ 2019 July: Vol. 08 No. 02 P: 30-35

STUDIES ON THE PERFORMANCE, DEVELOPMENT AND CARCASS COMPOSITION OF THE GROWING PIG: EFFECTS OF SEX, FEEDING REGIME AND AGE ON BLOOD SERUM PARAMETERS

1983 ◽  
Vol 63 (4) ◽  
pp. 977-984 ◽  
Author(s):  
H. DOORNENBAL ◽  
A. K. W. TONG ◽  
A. H. MARTIN ◽  
A. P. SATHER
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Serum Alkaline Phosphatase ◽  
Age Groups ◽  
Inorganic Phosphorus ◽  
Carcass Composition ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Parameters ◽  
Feeding Regime

Blood samples from 120 pigs serially slaughtered at five age groups, starting at 85 days of age with intervals of 2 wk, were used to study the effects of sex, feeding regime and age on a 12 component metabolic blood serum profile. The differences between barrows and gilts were small and only significant (P < 0.05) for blood urea. Pigs fed ad libitum had a significantly (P < 0.05) higher level of blood urea, calcium, inorganic phosphorus and alkaline phosphatase but a significantly (P < 0.01) lower level of creatinine than restricted fed pigs. Age had the most pronounced effects on the serum parameters studied, with blood urea, calcium, bilirubin, creatinine, total serum protein and albumin showing a significant (P < 0.01) increase per unit of serum with increasing age. The serum glucose, inorganic phosphorus, glutamic oxaloacetic transaminase and lactate dehydrogenase were also significantly (P < 0.01, P < 0.05 for glucose) influenced by age but did not show a consistent trend across the age groups. Serum alkaline phosphatase was the only component which significantly (P < 0.01) decreased with age. The results indicate that when these serum components are to be used for diagnostic or comparison purposes, age adjustment must be applied. Key words: Blood parameters, pigs, age, feeding regime, sex

Sign in / Sign up

Export Citation Format

Share Document