Key role of the expression of bone morphogenetic proteins in increasing the osteogenic activity of osteoblast-like cells exposed to shock waves and seeded on bioactive glass-ceramic scaffolds for bone tissue engineering

2014 ◽  
Vol 29 (5) ◽  
pp. 728-736 ◽  
Author(s):  
Giuliana Muzio ◽  
Germana Martinasso ◽  
Francesco Baino ◽  
Roberto Frairia ◽  
Chiara Vitale-Brovarone ◽  
...  
Keyword(s):  
Shock Wave ◽  
Alkaline Phosphatase ◽  
Shock Waves ◽  
Bioactive Glass ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Proteins ◽  
Glass Ceramic ◽  
Osteogenic Activity ◽  
Morphogenetic Protein

In this work, the role of shock wave-induced increase of bone morphogenetic proteins in modulating the osteogenic properties of osteoblast-like cells seeded on a bioactive scaffold was investigated using gremlin as a bone morphogenetic protein antagonist. Bone-like glass-ceramic scaffolds, based on a silicate experimental bioactive glass developed at the Politecnico di Torino, were produced by the sponge replication method and used as porous substrates for cell culture. Human MG-63 cells, exposed to shock waves and seeded on the scaffolds, were treated with gremlin every two days and analysed after 20 days for the expression of osteoblast differentiation markers. Shock waves have been shown to induce osteogenic activity mediated by increased expression of alkaline phosphatase, osteocalcin, type I collagen, BMP-4 and BMP-7. Cells exposed to shock waves plus gremlin showed increased growth in comparison with cells treated with shock waves alone and, conversely, mRNA contents of alkaline phosphatase and osteocalcin were significantly lower. Therefore, the shock wave-mediated increased expression of bone morphogenetic protein in MG-63 cells seeded on the scaffolds is essential in improving osteogenic activity; blocking bone morphogenetic protein via gremlin completely prevents the increase of alkaline phosphatase and osteocalcin. The results confirmed that the combination of glass-ceramic scaffolds and shock waves exposure could be used to significantly improve osteogenesis opening new perspectives for bone regenerative medicine.

scholarly journals Bone marrow-derived heparan sulfate potentiates the osteogenic activity of bone morphogenetic protein-2 (BMP-2)

Bone ◽  
2012 ◽  
Vol 50 (4) ◽  
pp. 954-964 ◽  
Author(s):  
Diah S. Bramono ◽  
Sadasivam Murali ◽  
Bina Rai ◽  
Ling Ling ◽  
Wei Theng Poh ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Heparan Sulfate ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Osteogenic Activity ◽  
Morphogenetic Protein

scholarly journals The role of bone morphogenetic protein(BMP)cytokine family in folliculogenesis

2017 ◽  
Vol 32 (0) ◽  
pp. 7-12
Author(s):  
Osamu Yoshino ◽  
Ikumi Akiyama ◽  
Yutaka Osuga ◽  
Shigeru Saito
Keyword(s):  
Bone Morphogenetic Protein ◽  
Morphogenetic Protein

scholarly journals Oocyte-Specific Overexpression of Mouse Bone Morphogenetic Protein-15 Leads to Accelerated Folliculogenesis and an Early Onset of Acyclicity in Transgenic Mice

Endocrinology ◽  
2008 ◽  
Vol 149 (6) ◽  
pp. 2807-2815 ◽  
Author(s):  
Heather E. McMahon ◽  
Osamu Hashimoto ◽  
Pamela L. Mellon ◽  
Shunichi Shimasaki
Keyword(s):  
Transgenic Mice ◽  
Bone Morphogenetic Protein ◽  
Early Onset ◽  
Ovarian Function ◽  
Chimeric Protein ◽  
Mature Protein ◽  
Follicle Growth ◽  
Morphogenetic Protein

Whereas mutations in the bmp15 gene cause infertility in ewes and women due to defects in folliculogenesis, most defects in female mice lacking bone morphogenetic protein (BMP)-15 are confined to the ovulation process, supportive of the observation that functional mouse BMP-15 is barely detected in oocytes in vivo until after the LH surge. In addition, the mouse BMP-15 proprotein is not processed into the functional mature protein in transfected cells. However, a chimeric protein consisting of the human proregion, human cleavage site, and mouse mature region (termed hhmBMP-15) is processed and the mature protein secreted. To study the role of BMP-15 in folliculogenesis, we generated transgenic mice overexpressing hhmBMP-15, exclusively in oocytes during folliculogenesis and confirmed the overexpression of mouse BMP-15 mature protein. Immature transgenic mice exhibited accelerated follicle growth with decreased primary follicles and an increase in secondary follicles. Granulosa cells of immature mice displayed an increased mitotic index and decreased FSH receptor mRNA expression. Adult mice had normal litter sizes but an increased number of atretic antral follicles. Interestingly, aging mice exhibited an early onset of acyclicity marked by increased diestrus length and early occurrence of constant diestrus. These findings indicate the role of BMP-15 in vivo in promoting follicle growth and preventing follicle maturation, resulting in an early decline in the ovarian reserve of transgenic mice. Therefore, the lack of mouse BMP-15 during early folliculogenesis in the wild-type mice may be relevant to their polyovulatory nature as well as the preservation of ovarian function as the mice age.

Sign in / Sign up

Export Citation Format

Share Document