Quantitative wear particle analysis for osteoarthritis assessment

2017 ◽  
Vol 231 (12) ◽  
pp. 1116-1126
Author(s):  
Meizhai Guo ◽  
Megan S Lord ◽  
Zhongxiao Peng
Keyword(s):  
Wear Debris ◽  
Wear Particle ◽  
Degradation Process ◽  
Cartilage Degradation ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Particle Analysis ◽  
Wear Particles ◽  
Wear Particle Analysis ◽  
Invasive Method

Osteoarthritis is a degenerative joint disease that affects millions of people worldwide. The aims of this study were (1) to quantitatively characterise the boundary and surface features of wear particles present in the synovial fluid of patients, (2) to select key numerical parameters that describe distinctive particle features and enable osteoarthritis assessment and (3) to develop a model to assess osteoarthritis conditions using comprehensive wear debris information. Discriminant analysis was used to statistically group particles based on differences in their numerical parameters. The analysis methods agreed with the clinical osteoarthritis grades in 63%, 50% and 61% of particles for no osteoarthritis, mild osteoarthritis and severe osteoarthritis, respectively. This study has revealed particle features specific to different osteoarthritis grades and provided further understanding of the cartilage degradation process through wear particle analysis – the technique that has the potential to be developed as an objective and minimally invasive method for osteoarthritis diagnosis.

Wear Particle Characterization and Severity Evaluation of Perodua Myvi 1.3L Automatic Transmission (AT) Fluid

2013 ◽  
Vol 393 ◽  
pp. 913-918
Author(s):  
Syazuan Abdul Latip ◽  
Salmiah Kasolang ◽  
Siti Khadijah Alias ◽  
Amirul Abd Rashid ◽  
Abdul Hakim Abdullah ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Automatic Transmission ◽  
Surface Characteristics ◽  
Wear Particle ◽  
Wear Behaviour ◽  
Particle Analysis ◽  
Wear Particles ◽  
Wear Particle Analysis ◽  
Roughness Analysis ◽  
Operating Distance

s. This paper investigates the characteristic and severity level of both wear and wear particles occurred in Perodua MyVi 1300cc automatic transmission (AT) mechanism via wear particle analysis approach. The analyses deployed were based on ferrographic and surface roughness analysis. The work of analysis strictly conducted on automatic transmission fluid (ATF) Perodua original equipment manufacturer (OEM) (ATF-3) series via continuous endurance dynamometer basis at the operating speed of 3000rpm. The operating mileage tested ranged from 0km up to 10,000km maximum operating distance. The wear particle generated at each operating mileage of 1,500km, 3,000km, 4,500km, 7,000km and 10,000km was accordingly analyzed morphologically and qualitatively. Ferrographic analysis is by principal has been recognized as one of the most reliable analysis incorporated with wear particle analysis (WPA) concern [1]. In concern of this study, it is applied to examine the morphology, mode and characteristic of wear particles generated. The surface roughness analysis meanwhile conducted to qualitatively evaluate and predict the wear condition of components within the AT mechanism via qualitative surface texture analysis of the wear particles. The outcome from the investigation done on the wear particles surface characteristics could interpret the wear behaviour and progress (stage/phase) as the surface characteristics of the wear particles do depict the surface characteristics of the wear components [2, 3].

Wear Particle Analysis, the Result of Tribological Phenomena of Biomaterials Couplings-Ultra Hight Molecular Weight Polyethylene and Magnesium Aluminosilicate Ceramic

2014 ◽  
Vol 216 ◽  
pp. 226-230
Author(s):  
Ciprian Stamate ◽  
Corneliu Munteanu ◽  
Monica Cretan Stamate
Keyword(s):  
Molecular Weight ◽  
Saline Solution ◽  
Wear Particle ◽  
Movement Speed ◽  
Particle Analysis ◽  
Wear Particles ◽  
Wear Particle Analysis ◽  
Biomaterial Surfaces ◽  
Circular Surface ◽  
Metal Polymer

Joint prosthesis involves the combination in the form of friction couplings of the different materials such as two metals, metal-polymer and polymer-ceramic. This paper aims to analyze the wear particles produced from the friction between two biomaterials: ultra hight molecular weight polyethylene( uhmwpe ) and magnesium aluminosilicate ceramic. To obtain the wear particles using a tribometer cetr umt-2 type that can reproduce the wear phenomena of different biomaterials in different humidity conditions and at various speeds of movement. The tests were performed in dry and lubricated friction conditions using saline solution, the best imitating biological conditions. The movement is similar to the friction torques of the hip joint: a semicircular motion of the polymer piece on the circular surface of the ceramic piece. Movement speed chosen is 0.25 m/s. Wear particles obtained were isolated from biomaterial surfaces and studied using scanning electron microscope. The images obtained are analyzed, particle's dimensions are extracted and then imported into autocad can provide information about the shape, size and surface of the particles. In light of these facts, we can say the inflammatory risk of biomaterial particles. In conclusion, the biomaterial particles are very small and the inflammatory rick is reducing to minimum in lubricated conditions.

Vitamin E-infused highly cross-linked polyethylene did not reduce the number of in vivo wear particles in total knee arthroplasty

2020 ◽  
Vol 102-B (11) ◽  
pp. 1527-1534
Author(s):  
Kumi Orita ◽  
Yukihide Minoda ◽  
Ryo Sugama ◽  
Yoichi Ohta ◽  
Hideki Ueyama ◽  
...  
Keyword(s):  
Total Knee Arthroplasty ◽  
Vitamin E ◽  
Polyethylene Wear ◽  
Wear Particle ◽  
Particle Analysis ◽  
Wear Particles ◽  
Wear Particle Analysis ◽  
Total Knee

Aims Vitamin E-infused highly cross-linked polyethylene (E1) has recently been introduced in total knee arthroplasty (TKA). An in vitro wear simulator study showed that E1 reduced polyethylene wear. However there is no published information regarding in vivo wear. Previous reports suggest that newly introduced materials which reduce in vitro polyethylene wear do not necessarily reduce in vivo polyethylene wear. To assist in the evaluation of the newly introduced material before widespread use, we established an in vivo polyethylene wear particle analysis for TKA. The aim of this study was to compare in vivo polyethylene wear particle generation between E1 and conventional polyethylene (ArCom) in TKA. Methods A total of 34 knees undergoing TKA (17 each with ArCom or E1) were investigated. Except for the polyethylene insert material, the prostheses used for both groups were identical. Synovial fluid was obtained at a mean of 3.4 years (SD 1.3) postoperatively. The in vivo polyethylene wear particles were isolated from the synovial fluid using a previously validated method and examined by scanning electron microscopy. Results The total number of polyethylene wear particles obtained from the knees with E1 (mean 6.9, SD 4.0 × 107 counts/knee) was greater than that obtained from those with ArCom (mean 2.2, SD 2.6 × 107 counts/knee) (p = 0.001). The particle size (equivalent circle of diameter) from the knees with E1 was smaller (mean 0.5 μm, SD 0.1) than that of knees with ArCom (mean 1.5, SD 0.3 μm) (p = 0.001). The aspect ratio of particles from the knees with E1 (mean 1.3, SD 0.1) was smaller than that with ArCom (mean 1.4, SD 0.1) (p < 0.001 ). Conclusion This is the first report of in vivo wear particle analysis of E1. E1 polyethylene did not reduce the number of in vivo polyethylene wear particles compared with ArCom in early clinical stage. Further careful follow-up of newly introduced E1 for TKA should be carried out. Cite this article: Bone Joint J 2020;102-B(11):1527–1534.

Fault diagnosis through Ferrography (Wear Particle Analysis)

2021 ◽  
pp. 303-322
Author(s):  
Anadi Sinha
Keyword(s):  
Nuclear Power ◽  
Power Plants ◽  
Nuclear Power Plants ◽  
Wear Particle ◽  
Stage Ii ◽  
Lubricating Oil ◽  
Particle Analysis ◽  
Wear Particle Analysis ◽  
Analysis Technique ◽  
Detection And Diagnosis

The purpose of Plant Predictive Maintenance (PDM) programme is to improve Reliability of machineries through early detection and diagnosis of equipment problems, and degradation prior to equipment failure. Ferrography (Wear Particle Analysis) is one of the PDM techniques which allows detection, identification and evaluation of the degradation at the very incipient stage so that degradation is timely attended and mitigatory actions initiated. Ferrography is a Wear Particle Analysis technique based upon systematic collection and analysis of sample of lubricating oil from rotating and reciprocating machines. Ferrography analysis is conducted in 2 phases: Stage I – Quantitative, and Stage II – Qualitative. After Stage II analysis, recommendation is issued based on wear rating (Normal, Marginal, or Critical) so that operator can take timely action. Presently, 21 Nuclear Power Plants are operational in India and Forced Shutdown is a very costly affair. Lube oil of around 60 equipment from Indian Nuclear Power Plants is examined quarterly for Ferrography analysis, and failure of several equipment is avoided due to timely action. This paper will elaborate on the basic principles of Ferrography, and how systematic implementation of Ferrography has helped in avoiding forced failure of equipment, and hence prevent Forced Shutdown.

scholarly journals A simple method of suspending implant wear particles for more physiological modelling of cell-particle interactions in vitro

2021 ◽  
Author(s):  
Christine Poon
Keyword(s):  
Wear Debris ◽  
Culture Media ◽  
Polymeric Materials ◽  
Wear Particle ◽  
Physicochemical Characteristics ◽  
Wear Particles ◽  
Simple Method ◽  
Implant Wear

AbstractArthroplasty implants e.g. hip, knee, spinal disc sustain relatively high compressive loading and friction wear, which lead to the formation of wear particles or debris between articulating surfaces. Despite advances in orthopaedic materials and surface treatments, the production of wear debris from any part of a joint arthroplasty implant is currently unavoidable. Implant wear debris induces host immune responses and inflammation, which causes patient pain and ultimately implant failure through progressive inflammation-mediated osteolysis and implant loosening, where the severity and rate of periprosthetic osteolysis depends on the material and physicochemical characteristics of the wear particles. Evaluating the cytotoxicity of implant wear particles is important for regulatory approved clinical application of arthroplasty implants, as is the study of cell-particle response pathways. However, the wear particles of polymeric materials commonly used for arthroplasty implants tend to float when placed in culture media, which limits their contact with cell cultures. This study reports a simple means of suspending wear particles in liquid medium using sodium carboxymethyl cellulose (NaCMC) to provide a more realistic proxy of the interaction between cells and tissues to wear particles in vivo, which are free-floating in synovial fluid within the joint cavity. Low concentrations of NaCMC dissolved in culture medium were found to be effective for suspending polymeric wear particles. Such suspensions may be used as more physiologically-relevant means for testing cellular responses to implant wear debris, as well as studying the combinative effects of shear and wear particle abrasion on cells in a dynamic culture environments such as perfused tissue-on-chip devices.

scholarly journals Selonsertib Alleviates the Progression of Rat Osteoarthritis: An in vitro and in vivo Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiyuan Yan ◽  
Yingchi Zhang ◽  
Gaohong Sheng ◽  
Bowei Ni ◽  
Yifan Xiao ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Cartilage Damage ◽  
Cartilage Degradation ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Therapeutic Effects ◽  
Exact Role

Osteoarthritis (OA) is a prevalent degenerative joint disease. Its development is highly associated with inflammatory response and apoptosis in chondrocytes. Selonsertib (Ser), the inhibitor of Apoptosis Signal-regulated kinase-1 (ASK1), has exhibited multiple therapeutic effects in several diseases. However, the exact role of Ser in OA remains unclear. Herein, we investigated the anti-arthritic effects as well as the potential mechanism of Ser on rat OA. Our results showed that Ser could markedly prevent the IL-1β-induced inflammatory reaction, cartilage degradation and cell apoptosis in rat chondrocytes. Meanwhile, the ASK1/P38/JNK and NFκB pathways were involved in the protective roles of Ser. Furthermore, intra-articular injection of Ser could significantly alleviate the surgery induced cartilage damage in rat OA model. In conclusion, our work provided insights into the therapeutic potential of Ser in OA, indicating that Ser might serve as a new avenue in OA treatment.

LncRNA MEG3 Inhibits the Degradation of the Extracellular Matrix of Chondrocytes in Osteoarthritis via Targeting miR-93/TGFBR2 Axis

Cartilage ◽  
2019 ◽  
pp. 194760351985575 ◽  
Author(s):  
Kang Chen ◽  
Hao Zhu ◽  
Min-Qian Zheng ◽  
Qi-Rong Dong
Keyword(s):  
Extracellular Matrix ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Cartilage Degradation ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Regulatory Function ◽  
Qrt Pcr ◽  
Ecm Degradation ◽  
Competitive Endogenous Rna

Background As a degenerative joint disease, osteoarthritis (OA) is characterized by articular cartilage degradation. Long noncoding RNAs (lncRNAs) act critical roles in the regulation of OA development, including affecting the proliferation, apoptosis, extracellular matrix (ECM) degradation, and inflammatory response of chondrocytes. The current study’s aim was to investigate the regulatory function and the underlying molecular mechanism of lncRNA MEG3 in ECM degradation of chondrocytes in OA. Methods In the current study, chondrocytes were induced by interleukin-1β (IL-1β) to simulate OA condition, and further assessed cell viability, lncRNA MEG3 and miR-93 expression levels. Overexpression or knockdown of lncRNA MEG3 in chondrocytes treated with IL-1β were performed to investigate the function of MEG3 in regulating cell proliferation, apoptosis and ECM degradation using EdU assay, flow cytometry, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western blot. The interaction between MEG3 and miR-93 was assessed using qRT-PCR. Furthermore, overexpression of miR-93 was performed as recovery experiment to explore the functional mechanism of MEG3. Results MEG3 was significantly downregulated in chondrocytes treated with IL-1β, whereas miR-93 was upregulated concomitantly. Overexpression of MEG3 induced the proliferation, suppressed the apoptosis, and relieved the degradation of ECM in IL-1β-induced chondrocytes. By contrast, knockdown of MEG3 suppressed the proliferation, promoted the apoptosis, and aggravated ECM degradation in IL-1β induced chondrocytes. In addition, MEG3 was found to relieve the inhibitive expression of TGFBR2 as a competitive endogenous RNA (ceRNA) of miR-93, and then activated transforming growth factor-β (TGF-β) signaling pathway, regulated chondrocytes ECM degradation in IL-1β induced chondrocytes subsequently. Conclusion LncRNA MEG3 targeted miR-93/TGFBR2 axis, regulated the proliferation, apoptosis and ECM degradation of chondrocytes in OA.

scholarly journals New Therapeutic Strategies for Osteoarthritis by Targeting Sialic Acid Receptors

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 637 ◽  
Author(s):  
Paula Carpintero-Fernandez ◽  
Marta Varela-Eirin ◽  
Alessandra Lacetera ◽  
Raquel Gago-Fuentes ◽  
Eduardo Fonseca ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Ex Vivo ◽  
Molecular Model ◽  
Cartilage Degradation ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Novel Therapy ◽  
Human Cartilage ◽  
Sialic Acid Receptors ◽  
Cartilage Breakdown

Osteoarthritis (OA) is the most common degenerative joint disease characterized by articular cartilage degradation and joint degeneration. The articular cartilage is mainly formed by chondrocytes and a collagen-proteoglycan extracellular matrix that contains high levels of glycosylated proteins. It was reported that the shift from glycoproteins containing α-2,6-linked sialic acids to those that contain α-2,3 was associated with the onset of common types of arthritis. However, the pathophysiology of α-2,3-sialylation in cartilage has not been yet elucidated. We show that cartilage from osteoarthritic patients expresses high levels of the α-2,3-sialylated transmembrane mucin receptor, known as podoplanin (PDPN). Additionally, the Maackia amurensis seed lectin (MASL), that can be utilized to target PDPN, attenuates the inflammatory response mediated by NF-kB activation in primary chondrocytes and protects human cartilage breakdown ex vivo and in an animal model of arthritis. These findings reveal that specific lectins targeting α-2,3-sialylated receptors on chondrocytes might effectively inhibit cartilage breakdown. We also present a computational 3D molecular model for this interaction. These findings provide mechanistic information on how a specific lectin could be used as a novel therapy to treat degenerative joint diseases such as osteoarthritis.

The Effect of the Prethanol Extract of Trifolium pratense Leaves on Interleukin-1β-Induced Cartilage Matrix Degradation in Primary Rat Chondrocytes

2018 ◽  
Vol 206 (1-2) ◽  
pp. 95-105 ◽  
Author(s):  
Seul Ah Lee ◽  
Sung-Min Moon ◽  
Seul Hee Han ◽  
Jae-Sung Kim ◽  
Do Kyung Kim ◽  
...  
Keyword(s):  
Trifolium Pratense ◽  
Nuclear Translocation ◽  
Red Clover ◽  
Cartilage Degradation ◽  
Degenerative Joint Disease ◽  
Mitogen Activated Protein Kinase ◽  
Joint Disease ◽  
Griess Reagent ◽  
Mitogen Activated Protein ◽  
Cox 2

Background: Osteoarthritis (OA) is a degenerative joint disease, characterized by cartilage degradation and inflammation. The proinflammatory cytokine, interleukin (IL)-1β, plays a crucial role in the pathogenesis of OA by inducing the release of other catabolic factors that contribute to cartilage degradation. Trifolium pratense L. (red clover) has been used as a medicinal plant in many countries and as a source of nutraceuticals to alleviate the symptoms of menopause. Ob­jectives: In this study, we aimed to evaluate the anticatabolic effect of 40% prethanol extract of T. pratense (40% PeTP) on IL-1β-stimulated chondrocytes. Methods: Primary rat chondrocytes were pretreated with 40% PeTP for 1 h before stimulation with IL-1β (20 ng/mL). The production of nitrite, prostaglandin E2 (PGE2), and aggrecan was measured by using Griess reagent and ELISA. Protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, A disintegrin and metalloproteinase with thrombospondin motif (ADAMTS)-4, mitogen-activated protein kinase (MAPK), and the nuclear factor (NF)-κB p65 subunit was measured by using Western blotting. Results: PeTP (40%) significantly inhibited the IL-1β-induced expression of nitrite, iNOS, PGE2, COX-2, MMP-1, MMP-3, MMP-13, and ADAMTS-4 in isolated primary rat chondrocytes. Furthermore, 40% PeTP decreased the IL-1β-induced degradation of aggrecan, the phosphorylation of MAPKs, and the nuclear translocation of the NF-κB p65 subunit. Conclusion: These results suggested that 40% PeTP has a chondroprotective effect on inflammation and may be a potential preventative agent for OA progression.

Sign in / Sign up

Export Citation Format

Share Document