scholarly journals Overexpression of miR-10b in colorectal cancer patients: Correlation with TWIST-1 and E-cadherin expression

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769591 ◽  
Author(s):  
Rania Abdelmaksoud-Dammak ◽  
Nour Chamtouri ◽  
Mouna Triki ◽  
Amena Saadallah-Kallel ◽  
Wajdi Ayadi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Messenger Rna ◽  
Protein Levels ◽  
Adjacent Mucosa ◽  
Cellular Pathways ◽  
Aberrant Dna Methylation ◽  
Twist 1 ◽  
Colorectal Cancer Patients ◽  
Membranous Protein ◽  
E Cadherin

MicroRNAs are emergent players of epigenetics that function as oncogenes or tumor suppressors and that have been implicated in regulating diverse cellular pathways. MiR-10b is an oncogenic microRNA involved in tumor invasion and metastasis in various cancers. Our data have shown that miR-10b is overexpressed in colorectal cancer samples in comparison with non-tumorous adjacent mucosa (p = 0.0025) and that it is associated with severe features such as tumor size >5 cm (p = 0.023), distant metastasis (p = 0.0022), non-differentiated tumors (p = 0.016), and vascular invasion (p = 0.01). Regarding the regulation of its expression, positive correlation between the loss of miR-10b and aberrant DNA methylation (p = 0.02) as well as a loss of TWIST-1 messenger RNA (p = 0.018) have been observed. Furthermore, expression analysis of the downstream miR-10b targets has shown that there are associations between low HOXD10 messenger RNA and E-cadherin protein levels (p < 0.0001, p = 0.0008, respectively) and overexpression of miR-10b. Our data suggests that overexpression of miR-10b results from high levels of TWIST-1 and may induce a decrease of E-cadherin membranous protein levels, thus contributing to the acquisition of metastatic phenotypes in colorectal cancer.

Soluble serum E-cadherin as a marker of tumour progression in colorectal cancer patients

2004 ◽  
Vol 21 (1) ◽  
pp. 75-78 ◽  
Author(s):  
C. Wilmanns ◽  
J. Grossmann ◽  
S. Steinhauer ◽  
G. Manthey ◽  
B. Weinhold ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Tumour Progression ◽  
Colorectal Cancer Patients ◽  
E Cadherin

Microsomal prostaglandin E synthase protein levels correlate with prognosis in colorectal cancer patients

2009 ◽  
Vol 454 (6) ◽  
pp. 667-676 ◽  
Author(s):  
Tsuguhiko Seo ◽  
Atsushi Tatsuguchi ◽  
Seiichi Shinji ◽  
Masaoki Yonezawa ◽  
Keigo Mitsui ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Prostaglandin E ◽  
Prostaglandin E Synthase ◽  
Protein Levels ◽  
Colorectal Cancer Patients ◽  
Microsomal Prostaglandin E Synthase

scholarly journals LRIG1 Expression and Colorectal Cancer Prognosis

2020 ◽  
Author(s):  
Maryam Bakherad ◽  
Mahdieh Salimi ◽  
Seyed Abdolhamid Angaji ◽  
Frouzandeh Mahjoubi ◽  
Tayebeh Majidizadeh
Keyword(s):  
Colorectal Cancer ◽  
Prognostic Significance ◽  
Prognostic Indicator ◽  
Cancer Prognosis ◽  
Protein Levels ◽  
Tumor Tissues ◽  
The Right ◽  
Colorectal Cancer Patients ◽  
Leucine Rich Repeats ◽  
And Control

Abstract BackgroundTo make the right treatment decisions about colorectal cancer (CRC) patients reliable predictive and prognostic data are needed. However, in many cases this data is not enough. Some studies suggest that LRIG1 gene (leucine-rich repeats and immunoglobulin-like domains1) has prognostic implications in different kinds of cancers. MethodsOne hundred and two patients with colorectal cancer were retrospectively analyzed for LRIG1 expression at both mRNA and protein levels. SYBR Green Real-Time RT-PCR technique was used for mRNA expression analyses and Glyceraldehyde-3-Phosphate Dehydrogenase gene (GAPDH) was considered as a reference gene for data normalization. LRIG1 protein expression was analyzed using Immunohistochemistry (IHC). Additionally, appropriate statistic analyses were used to assess the expression of LRIG1 in test and control groups. The prognostic significance of LRIG1 expression was analyzed using the univariate and multivariate analyses.ResultsThe data revealed that the expression of LRIG1 in both mRNA and protein levels was down regulated in colorectal tumor tissues (P<0.01) but is not clinically relevant prognostic indicator in CRC. ConclusionsTherefore, it is suggested that LRIG1 expression analyses may not be considered as an important issue when making informed and individualized clinical decisions regarding the management of colorectal cancer patients.

scholarly journals Colorectal cancer patients with different C-reactive protein levels and 5-year survival times can be differentiated with quantitative serum proteomics

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0195354 ◽  
Author(s):  
Matilda Holm ◽  
Mayank Saraswat ◽  
Sakari Joenväärä ◽  
Ari Ristimäki ◽  
Caj Haglund ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Survival Times ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein ◽  
Serum Proteomics ◽  
Colorectal Cancer Patients

scholarly journals Cyclooxygenase (COX)-1, Cyclooxygenase (COX)-2 and E-cadherin Expression in Colorectal Cancer Patients with Hepatic Metastasis

2010 ◽  
Vol 78 (3) ◽  
pp. 177
Author(s):  
Sun Hyung Joo ◽  
Bum Soo Kim ◽  
Sung Il Choi ◽  
Jeong Yoon Song ◽  
Kil Yeon Lee ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Hepatic Metastasis ◽  
Cox 2 ◽  
Colorectal Cancer Patients ◽  
Cox 1 ◽  
E Cadherin

Abstract 1470: Relative telomere length in tumor tissue and adjacent mucosa of colorectal cancer patients

2018 ◽  
Author(s):  
Michal Kroupa ◽  
Vaclav Liska ◽  
Krishna Rachakonda ◽  
Marketa Urbanova ◽  
Michaela Schneiderova ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Telomere Length ◽  
Cancer Patients ◽  
Tumor Tissue ◽  
Adjacent Mucosa ◽  
Relative Telomere Length ◽  
Colorectal Cancer Patients

scholarly journals The Differential DNA Hypermethylation Patterns of microRNA-137 and microRNA-342 Locus in Early Colorectal Lesions and Tumours

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 519
Author(s):  
Elham Kashani ◽  
Mahrooyeh Hadizadeh ◽  
Vahid Chaleshi ◽  
Reza Mirfakhraie ◽  
Chris Young ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Methylation Status ◽  
Dna Hypermethylation ◽  
Normal Colon Mucosa ◽  
Normal Tissues ◽  
Diagnosis And Prognosis ◽  
Adjacent Mucosa ◽  
Prediction And Prevention ◽  
Colorectal Cancer Patients

Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide, representing 13% of all cancers. The role of epigenetics in cancer diagnosis and prognosis is well established. MicroRNAs in particular influence numerous cancer associated processes including apoptosis, proliferation, differentiation, cell-cycle controls, migration/invasion and metabolism. MiRNAs-137 and 342 are exon- and intron-embedded, respectively, acting as tumour-suppressive microRNA via hypermethylation events. Levels of miRNAs 137 and 342 have been investigated here as potential prognostic markers for colorectal cancer patients. The methylation status of miRNA-137 and miRNA-342 was evaluated using methylation-specific (MSP) polymerase chain reaction (PCR) on freshly frozen tissue derived from 51 polyps, 8 tumours and 14 normal colon mucosa specimens. Methylation status of miRNA-137 and miRNA-342 was significantly higher in tumour lesions compared to normal adjacent mucosa. Surprisingly, the methylation frequency of miR-342 (76.3%) among colorectal cancer patients was significantly higher compared to miR-137 (18.6%). Furthermore, normal tissues, adjacent to the lesions (N-Cs), displayed no observable methylation for miRNA-137, whereas 27.2% of these N-Cs showed miRNA-342 hypermethylation. MiRNA-137 hypermethylation was significantly higher in male patients and miR-342 hypermethylation correlated with patient age. Methylation status of miRNA-137 and miRNA-342 has both diagnostic and prognostic value in CRC prediction and prevention.

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