Proteins Involved in the Induction of Procoagulant Activity and Autoimmune Response in Patients With Primary Antiphospholipid Syndrome

The aim of this study was to determine the plasma protein profile of patients with primary antiphospholipid syndrome (PAPS) compared to healthy controls and identify proteins that might be used in the evaluation, diagnosis, and prognosis of this condition. The sample consisted of 14 patients with PAPS and 17 sex- and age-matched controls. Plasma samples were submitted to proteomic analysis (albumin and immunoglobulin G depletion, concentration, digestion, and label-free data-independent mass spectrometry). The software ExpressionE was used to quantify intergroup differences in protein expression. The analysis yielded 65 plasma proteins of which 11 were differentially expressed (9 upregulated and 2 downregulated) in relation to controls. Four of these are known to play a role in pathophysiological mechanisms of thrombosis: fibrinogen α chain, fibrinogen α chain, apolipoprotein C-III, and α-1-glycoprotein-1. Our analysis revealed autoimmune response and the presence of proteins believed to be functionally involved in the induction of procoagulant activity in patients with PAPS. Further studies are necessary to confirm our findings and may eventually lead to the development of significantly more accurate diagnostic tools.

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ANTIPHOSPHATIDYLSERINE/PROTHROMBIN ANTIBODIES AS A RISK FACTOR OF DISEASE SEVERITY IN PRIMARY ANTIPHOSPHOLIPID SYNDROME

10.26226/morressier.56e174cfd462b8028d88a378 ◽

2016 ◽

Author(s):

Ariela Hoxha

Keyword(s):

Risk Factor ◽

Disease Severity ◽

Antiphospholipid Syndrome ◽

Primary Antiphospholipid Syndrome

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Preeclampsia in primary antiphospholipid syndrome is associated with fetal losses and limb ischemia

Clinical Rheumatology ◽

10.1007/s10067-021-05626-6 ◽

2021 ◽

Author(s):

Jozélio Freire de Carvalho ◽

Maria Betania Pereira Toralles ◽

Maria Isabel Figueiredo Sousa ◽

Thelma Larocca Skare

Keyword(s):

Antiphospholipid Syndrome ◽

Limb Ischemia ◽

Primary Antiphospholipid Syndrome

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In Vitro Spectroscopy-Based Profiling of Urothelial Carcinoma: A Fourier Transform Infrared and Raman Imaging Study

Cancers ◽

10.3390/cancers13010123 ◽

2021 ◽

Vol 13 (1) ◽

pp. 123

Author(s):

Monika Kujdowicz ◽

Wojciech Placha ◽

Brygida Mech ◽

Karolina Chrabaszcz ◽

Krzysztof Okoń ◽

...

Keyword(s):

Bladder Cancer ◽

Fourier Transform ◽

Cancer Cells ◽

Cell Line ◽

Cell Lines ◽

Malignant Neoplasm ◽

Fourier Transform Infrared ◽

Diagnostic Tools ◽

Label Free ◽

Bladder Cancer Cells

Markers of bladder cancer cells remain elusive, which is a major cause of the low recognition of this malignant neoplasm and its recurrence. This implies an urgent need for additional diagnostic tools which are based on the identification of the chemism of bladder cancer. In this study, we employed label-free techniques of molecular imaging—Fourier Transform Infrared and Raman spectroscopic imaging—to investigate bladder cancer cell lines of various invasiveness (T24a, T24p, HT-1376, and J82). The urothelial HCV-29 cell line was the healthy control. Specific biomolecules discriminated spatial distribution of the nucleus and cytoplasm and indicated the presence of lipid bodies and graininess in some cell lines. The most prominent discriminators are the total content of lipids and sugar moieties as well as the presence of glycogen and other carbohydrates, un/saturated lipids, cytochromes, and a level of S-S bridges in proteins. The combination of the obtained hyperspectral database and chemometric methods showed a clear differentiation of each cell line at the level of the nuclei and cytoplasm and pointed out spectral signals which differentiated bladder cancer cells. Registered spectral markers correlated with biochemical composition changes can be associated with pathogenesis and potentially used for the diagnosis of bladder cancer and response to experimental therapies.

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Cold Agglutinin-Mediated Autoimmune Hemolytic Anemia in Association with Antiphospholipid Syndrome

Acta Haematologica ◽

10.1159/000516295 ◽

2021 ◽

pp. 1-4

Author(s):

Ram Gelman ◽

Fadi Kharouf ◽

Yuval Ishay ◽

Alexander Gural

Keyword(s):

Hemolytic Anemia ◽

Antiphospholipid Syndrome ◽

Complement Activation ◽

Autoimmune Hemolytic Anemia ◽

Cold Agglutinin ◽

Primary Antiphospholipid Syndrome ◽

Unique Case ◽

Hematologic Disorders ◽

Clear Association ◽

Immune Mediated

Antiphospholipid syndrome and cold agglutinin-mediated autoimmune hemolytic anemia are 2 distinct immune-mediated hematologic disorders. While no clear association exists between these 2 entities, complement activation is known to occur in both of them. Herein, we report a unique case of cold agglutinin hemolytic anemia in a patient with a known primary antiphospholipid syndrome.

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Case of an unusual diagnosis of primary antiphospholipid syndrome with multiple clinical complications

Oxford Medical Case Reports ◽

10.1093/omcr/omaa117 ◽

2020 ◽

Vol 2020 (12) ◽

Author(s):

Stathis Tsiakas ◽

Chrysanthi Skalioti ◽

Paraskevi Kotsi ◽

Ioannis Boletis ◽

Smaragdi Marinaki

Keyword(s):

Antiphospholipid Syndrome ◽

Thrombotic Event ◽

Renal Involvement ◽

Clinical Manifestations ◽

Young Male ◽

Multiple Organ ◽

Systemic Autoimmune Disease ◽

Antiphospholipid Antibody ◽

Primary Antiphospholipid Syndrome ◽

Wide Range

ABSTRACT Antiphospholipid syndrome (APS) is a systemic autoimmune disease defined by the presence of antiphospholipid antibodies in association with thrombotic events and/or obstetric complications. Renal involvement is not infrequent in both primary and secondary APS. Kidney manifestations comprise a wide range of clinical features, including hypertension, major renal vessel thrombosis or microvascular endothelial injury, also described as APS nephropathy. In the absence of a thrombotic event, clinical manifestations of APS are often non-specific. We recently encountered a case of primary APS in a young male with newly diagnosed hypertension and renal impairment. The diagnosis of APS was initially suspected by his kidney biopsy findings, when electron microscopy examination showed the features of chronic microangiopathy, and was later confirmed by a triple positive antiphospholipid antibody profile and multiple organ involvement.

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