One vs two negative fungal cultures to confirm mycological cure in shelter cats treated for Microsporum canis dermatophytosis: a retrospective study

2019 ◽  
Vol 22 (6) ◽  
pp. 598-601
Author(s):  
Rebecca L Stuntebeck ◽  
Karen A Moriello
Keyword(s):  
Retrospective Study ◽  
Sampling Error ◽  
False Negative ◽  
Sampling Technique ◽  
Response To Treatment ◽  
Clinical Group ◽  
Microsporum Canis ◽  
Fungal Culture ◽  
Mycological Cure ◽  
Environmental Cleaning

Objectives The aim of this study was to determine how often one negative fungal culture (FC) was indicative of mycological cure (MC) when compared with two negative consecutive FCs in cats treated for Microsporum canis dermatophytosis. Methods In this retrospective study, weekly FC data from shelter cats treated for M canis dermatophytosis were reviewed. Results Complete records from 371 cats were reviewed. The first negative FC was indicative of MC in 335 (90.3%) cats. In this group, all cats were otherwise healthy and either had obvious lesions (n = 237) or no lesions or evidence of resolving lesions (n = 98). In the 36 cats in which the first negative culture was not indicative of MC, there were two clinical subgroups. The first consisted of healthy but lesional cats (n = 19) that had one negative FC within the first 3 weeks of treatment followed by one or more positive FCs. The most likely explanation was sampling error. These cats went on to cure and the next negative FC was indicative of MC. In the second clinical group, cats were lesional but had concurrent medical problems (n = 17). These cats showed an initial good response to treatment (lesion resolution and an initial negative FC). However, this negative FC was followed by at least one strongly positive FC (>10 colony-forming units/plate) before proceeding to cure. These cats took the longest time to cure (mean 11 weeks; range 8–28 weeks). MC occurred after resolution of the concurrent health issues. There was very good agreement between using one negative FC vs two negative FCs for the determination of MC in healthy cats (kappa = 0.903) Conclusions and relevance In cats where there has been high compliance with environmental cleaning, as well as topical and systemic treatment recommendations, two consecutive negative FCs may not be necessary to determine MC. The first negative FC in an otherwise healthy cat is likely indicative of MC. Good sampling technique is needed to avoid false-negative FC results.

Comparison of real-time PCR with fungal culture for the diagnosis of Microsporum canis dermatophytosis in shelter cats: a field study

2017 ◽  
Vol 20 (2) ◽  
pp. 103-107 ◽  
Author(s):  
Linda S Jacobson ◽  
Lauren McIntyre ◽  
Jenny Mykusz
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
False Positive ◽  
False Negative ◽  
Microsporum Canis ◽  
Fungal Culture ◽  
Mycological Cure ◽  
Hair Samples ◽  
Culture Positive ◽  
Culture Negative

Objectives Fungal culture requires at least 14 days for a final result, compared with 1–3 days for PCR. The study compared a commercial real-time dermatophyte PCR panel with fungal culture in cats in a shelter setting for: (1) diagnosis of Microsporum canis infection; and (2) determination of mycological cure. Methods This was a cross-sectional, observational study of cats with suspicious skin lesions or suspected exposure to dermatophytosis. Hair samples were collected for fungal culture and PCR prior to treatment and at weekly intervals until two negative culture results were obtained. Results One hundred and thirty-two cats were included, of which 28 (21.2%) were culture positive and 104 (78.8%) culture-negative for M canis. PCR correctly identified all culture-positive cats and 92/104 culture negative cats; there were 12 false-positive PCR results. The sensitivity and specificity of PCR were 100% (95% confidence interval [CI] 87.7–100) and 88.5% (95% CI 80.7–93.9), respectively. Data from 17 cats were available for assessment of mycological cure. At the time of the first and second negative fungal cultures, 14/17 (82.4%) and 11/17 (64.7%) tested PCR positive, respectively. Conclusions and relevance PCR showed high sensitivity and specificity for diagnosis of M canis dermatophytosis compared with fungal culture, but was unreliable for identifying mycological cure. False-positive results were relatively common. There were no false-negative PCR results and a negative PCR test was a reliable finding in this study. The ability to rapidly diagnose or rule out dermatophytosis could be a valuable tool to increase life-saving capacity in animal shelters.

scholarly journals Efficacy of itraconazole oral solution using an alternating-week pulse therapy regimen for treatment of cats with experimental Microsporum canis infection

2017 ◽  
Vol 20 (10) ◽  
pp. 869-874 ◽  
Author(s):  
Christopher Puls ◽  
Aaron Johnson ◽  
Karrie Young ◽  
Jonathan Hare ◽  
Kelly Rosenkrans ◽  
...  
Keyword(s):  
Clinical Cure ◽  
Pulse Therapy ◽  
Oral Solution ◽  
Controlled Study ◽  
Microsporum Canis ◽  
Fungal Culture ◽  
Mycological Cure ◽  
Therapy Regimen ◽  
Treatment Groups ◽  
Cure Rates

Objectives The objective of this study was to evaluate itraconazole 10 mg/ml oral solution for the treatment of Microsporum canis infection using an alternating-week pulse therapy regimen in a controlled laboratory setting. Methods Eighty cats with experimentally induced infections were randomly assigned to treatment (itraconazole vs control [sterile water]), administered 5 mg/kg PO q24h for 1 week on alternate weeks for 5 weeks, followed by a 4 week follow-up period. Topical therapeutic treatment was not administered. Cats were individually housed in stainless steel cages that were cleaned and disinfected daily. Study measures included weekly fungal cultures, clinical lesion scores, Wood’s lamp examination and periodic laboratory monitoring. Mycological cure was defined as two consecutive negative cultures. Results Itraconazole-treated cats had significantly greater ( P = 0.0003) mycological cure compared with untreated controls (24/40 [60%] vs 1/40 [2.5%], respectively) and all of these reached clinical cure and had negative final Wood’s lamp examinations. Furthermore, 36/40 (90%) treated cats had at least one negative fungal culture by the end of the study vs only 3/40 (7.5%) control cats. For both treatment groups, prevalence of clinical cure peaked at the end of the study (week 9), with 39/40 (97.5%) itraconazole-treated cats and 6/40 (15%) control cats achieving clinical cure. Wood’s lamp negative examination rates were significantly greater ( P <0.0001) for itraconazole-treated cats compared with controls (39/40 cats [97.5%] vs 6/40 [15%], respectively) and followed the same pattern of improvement as primary clinical lesions. Conclusions and relevance In this controlled study, orally administered itraconazole using a 5 mg/kg pulse-dose treatment regimen reduced the time to mycological cure and increased both mycological and clinical cure rates compared with untreated controls.

Evaluation of incubation time for Microsporum canis dermatophyte cultures

2017 ◽  
Vol 20 (10) ◽  
pp. 997-1000 ◽  
Author(s):  
Rebecca Stuntebeck ◽  
Karen A Moriello ◽  
Maria Verbrugge
Keyword(s):  
Retrospective Study ◽  
Incubation Time ◽  
Culture Medium ◽  
Skin Lesions ◽  
Post Treatment ◽  
Antifungal Treatment ◽  
Microsporum Canis ◽  
Fungal Culture ◽  
Abnormal Morphology ◽  
Colony Forming Units

Objectives The goal of this study was to determine how frequently Microsporum canis was isolated after 1, 2 and 3 weeks of incubation on dermatophyte culture medium either from untreated cats or cats during treatment. Methods This was an observational retrospective study. Toothbrush fungal culture results were examined from two data pools: untreated cats with suspect skin lesions and weekly fungal cultures from cats being treated for dermatophytosis. Results Results from 13,772 fungal cultures were reviewed and 2876 (20.9%) were positive for M canis. Of these, 2800 were confirmed as positive within 14 days of incubation and only 76 (2.6%) required >14 days for confirmation of M canis. In pretreatment specimens, 98.2% (1057/1076) of M canis isolates were recovered within 14 days of incubation in specimens from cats not known to have received prior antifungal treatment. For cats receiving treatment, 96.8% (1743/1800) of M canis isolates were recovered within 14 days of incubation. Of the 57 cultures that required >14 days for finalization, 21 required extra incubation time because cultures were grossly abnormal, 12 had concurrent contaminant growth delaying microscopic confirmation and 24 had no growth in the first 14 days. Of these 24, 19 had 1–2 colony-forming units (cfu)/plate and the remaining five plates had 5 to >10 cfu/plate, all with abnormal morphology. Conclusions and relevance The findings of this study show that it is not necessary to hold pretreatment or post-treatment fungal cultures for 21 days before finalizing cultures for no growth. Growth requiring >14 days had grossly abnormal morphology.

scholarly journals Treatment of children and adolescents with hemangioma using propranolol: preliminary results from a retrospective study

2014 ◽  
Vol 132 (1) ◽  
pp. 48-54 ◽  
Author(s):  
Juliana Costa Albuquerque ◽  
Rosane Aline Magalhaes ◽  
Jamille Araujo Felix ◽  
Maria Vilani Rodrigues Bastos ◽  
Juvenia Bezerra Fontenele ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Children And Adolescents ◽  
Previous Treatment ◽  
Capillary Hemangioma ◽  
Response To Treatment ◽  
Vascular Tumors ◽  
Lesion Volume ◽  
Chi Square ◽  
One Year ◽  
Almost All

CONTEXT AND OBJECTIVE: Hemangiomas are the commonest vascular tumors during childhood. In 2008, the effect of propranolol for treating capillary hemangiomas was demonstrated. Other similar results followed, showing that it rapidly reduces lesion volume. The objective here was to evaluate children and adolescents with hemangiomas that were treated with propranolol. DESIGN AND SETTING: Retrospective study, conducted in a children's hospital. METHODS : Patients aged 0-19 years with or without previous treatment, who were treated between January 2009 and December 2010, were included. The response was assessed by comparing the lesion appearance between the start of treatment and the last consultation. We considered partial or complete responses as the response to treatment. RESULTS : Sixty-nine patients with a median follow-up of 11 months (mean age: 31 months) were included. Of these, 58 patients were recently diagnosed and 11 had had previous treatment. A response (partial or complete) was seen in 60 patients (87%). Among the capillary hemangioma cases, responses were seen in 50 out of 53 (94%), while in other lesion types, it was 10 out of 16 (63%) (P = 0.3; chi-square). Responses in patients less than one year of age were seen in 37 out of 38 (97%), whereas in those over one year of age, in 23 out of 31 (74%) (P = 0.4; chi-square). Side effects were uncommon and mild. CONCLUSIONS: Propranolol seemed to be effective for treatment of hemangiomas in children and adolescents, and not just in the proliferative stage, with responses in almost all the patients.

scholarly journals A retrospective study of the health profile of neonates of mothers with anemia in pregnancy and pregnancy induced hypertension in Lagos, Nigeria

2014 ◽  
Vol 5 (2) ◽  
Author(s):  
Olusola Funmilayo Sotunde ◽  
Silifat Ajoke Sanni ◽  
Oluseye Olusegun Onabanjo ◽  
Ibiyemi O. Olayiwola ◽  
Mure Agbonlahor
Keyword(s):  
Retrospective Study ◽  
Birth Weight ◽  
Sampling Technique ◽  
Neonatal Health ◽  
Pregnancy Induced Hypertension ◽  
Health Profile ◽  
Maternal Complications ◽  
Induced Hypertension ◽  
Anemia In Pregnancy ◽  
In Pregnancy

Our study assessed the health profile of neonates in relation to anemia in pregnancy and pregnancy induced hypertension (PIH). This was a retrospective study where a systematic random sampling technique was used to select a total of 1046 case records of pregnant women registered for ante-natal care at Lagos Island Maternity Hospital, Lagos, Nigeria, between 2005 and 2009. Socio-demographic characteristics of the mothers, prevalence of anemia and PIH, and neonatal health profile were obtained from the case records and were analyzed using both descriptive and inferential statistics. Pearson product moment correlation was used to show the relationship (P≤0.05) between maternal complications and neonatal health profile. Majority (68.8%) of the mothers had anemia and 6.7 % had PIH. Majority (97.12%) of the neonates were live births and 2.88% of the neonates were still births, 65.4% of the women with still birth pregnancy outcome had anemia, and 34.6% had PIH. Majority (74%) of the neonates had birth weight within normal range (2.5-4.0 kg) and majority (68%) had normal Apgar score at 5 min of birth (7- 10). A positive correlation existed between the packed cell volume of the mother and the birth weight of the neonates (r=0.740, P≤0.05). A negative correlation existed between the incidence of PIH and the birth weight of the neonates (r=

scholarly journals PENGARUH PEMBERIAN ASI TERHADAP PERKEMBANGAN ANAK USIA 6 – 12 BULAN DI RW 04 DESA SAMBIBULU KECAMATAN TAMAN SIDOARJO

2015 ◽  
Vol 6 (2) ◽  
Author(s):  
F Firdaus ◽  
Ayun Nif’ah
Keyword(s):  
Retrospective Study ◽  
Child Development ◽  
Random Sampling ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Screening Questionnaire ◽  
Significance Level ◽  
Independent Variable

This research is to observe the purpose of this study was to analyze the influence of breastfeeding toward the development of children aged 6 - 12 months living in RW 04 Sambibulu Village, Taman Sub-district, Sidoarjo. The design of  research was analytic  retrospective  study. The population involved 29 couples with their children aged 6  -  12 months living in the above mentioned region. The simple random sampling technique was used to take 27 couples as the samples of research. The independent variable of this research was breastfeeding, whereas the dependent one was  the child’s development. Questionnaires of breast and checklist of KPSP (Pre Screening Questionnaire for Child Development) were used to collect the data which analyzed by using Mann-Whitney’s statistic test with the significance level α = 0.05, it means that there was a relationship between breastfeeding and  the development of children p (0.000) < α (0.05)

scholarly journals Gestational trophoblastic neoplasia: A 6 year retrospective study

2014 ◽  
Vol 03 (01) ◽  
pp. 033-037 ◽  
Author(s):  
Sushruta Shrivastava ◽  
Amal Chandra Kataki ◽  
Debabrata Barmon ◽  
Pankaj Deka ◽  
Chidananda Bhuyan ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Complete Remission ◽  
Risk Score ◽  
Single Agent ◽  
Response To Treatment ◽  
Gestational Trophoblastic Neoplasia ◽  
Response To Chemotherapy ◽  
Chemotherapy Patients ◽  
Line Chemotherapy

Abstract Aims and Objectives: To study the clinical presentations of gestational trophoblastic neoplasia and its response to chemotherapy. Materials and Methods: This is a retrospective study of 28 women of gestational trophoblastic neoplasia evaluated over a period of 6 years from January 2004 to December 2009. Patients were evaluated on the basis of their age, number of deliveries, history of abortion or molar pregnancy, and the treatment received. All patients were scored on the basis of WHO scoring system. Patients with low risk (score </=6) received single agent chemotherapy with methotrexate or actinomycin D. Patients with high risk (score >/=7) received multiple agent chemotherapy with EMACO regimen. After completion of chemotherapy patients were followed for a minimum of 2 years. The response to treatment was evaluated during follow-up by clinical examination, beta hCG levels and imaging as and when required. Results: Out of 28 women only 27 could be evaluated, because 1 patient was lost to follow-up. Out of 27 patients, 18 patients (66.67%) achieved complete remission with the first-line chemotherapy and additional 25.92% (7/27) achieved complete remission with second line chemotherapy resulting in complete remission of 92.5% (25/27). Conclusion: Gestational trophoblastic neoplasia is curable if patient is properly evaluated and scored. It shows good response to chemotherapy.

scholarly journals Cervical Cytology–Histology Correlation Based on the American Society of Cytopathology Guideline (2017) at the Russian National Medical Research Center for Obstetrics, Gynecology, and Perinatology

Diagnostics ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 210
Author(s):  
Aleksandra Asaturova ◽  
Darya Dobrovolskaya ◽  
Alina Magnaeva ◽  
Anna Tregubova ◽  
Guldana Bayramova ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Medical Research ◽  
Sampling Error ◽  
Pap Test ◽  
Research Center ◽  
National Medical ◽  
American Society ◽  
Screening Errors ◽  
Discrepancy Detection ◽  
Self Learning

Recent evidence suggests that a cytology–histology correlation (CHC) with discrepancy detection can both evaluate errors and improve the sensitivity and specificity of the cytologic method. We aimed to analyze the errors in cytologic–histologic discrepancies according to the CHC protocol guideline of the American Society of Cytopathology (2017). This retrospective study included 273 patients seen at the National Medical Research Center of Obstetrics, Gynecology and Perinatology (Moscow, Russia) between January 2019 and September 2021. The patients’ mean age was 34 ± 8.1 years. The cytology–histology agreement was noted in 158 cases (57.9%). Major discrepancies were found in 21 cases (7.6%), while minor discrepancies were noted in 93 cases (34.1%). The reason for 13 (4.8%) discrepancies was a colposcopy sampling error and, in 46 (16.8%) cases, the reason was a Papanicolaou (PAP) test sampling error. The discrepancy between primary and reviewed cytology was due interpretive errors in 13 (4.8%) cases and screening errors in 42 (15.4%) cases. We demonstrated that the ASC guidelines facilitate cervical CHC. A uniform application of these guidelines would standardize cervical CHCs internationally, provide a scope for the inter-laboratory comparison of data, and enhance self-learning and peer learning.

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