scholarly journals Evaluation of the Function of Wild Animal Gut Microbiomes Using Next-Generation Sequencing and Bioinformatics and its Relevance to Animal Conservation

2019 ◽  
Vol 15 ◽  
pp. 117693431984843 ◽  
Author(s):  
Ran Yao ◽  
Lianglaing Xu ◽  
Guoqing Lu ◽  
Lifeng Zhu
Keyword(s):  
Next Generation Sequencing ◽  
Gut Microbiome ◽  
Environmental Changes ◽  
Next Generation ◽  
Evolutionary Potential ◽  
Animal Conservation ◽  
Père David’S Deer ◽  
Fresh Feces ◽  
Gut Microbial Community ◽  
Generation Sequencing

The relationship between animal conservation and the animal gut microbiome is a hot topic in current microbial ecology research. Our group has recently revealed that the occurrence of diverse combinations of gut microbial compositions and functions (metagenomics) in Père David’s deer ( Elaphurus davidianus) populations is likely to lead to increased evolutionary potential and resilience in response to environmental changes. Thus, considering the effects of diet on the gut microbiome and the importance of a stable gut microbial community to host health, we suggest that a transitional buffer period (with feeding on a regular diet and a diet from the translocation habitat) is needed before animal translocation. When the gut microbiome enters into relatively stable stages and adapts to the new diet from the translocation site, the time is suitable for translocation. Long-term monitoring of the gut microbiomes of translocated animals (by collecting fresh feces and carrying out next-generation sequencing) is still necessary after their translocation.

MICROBIOMA: AMIGO O ENEMIGO EN PACIENTES CON COVID - 19

SCIENTIARVM ◽  
2015 ◽  
Vol 1 (1) ◽  
pp. 15-21
Author(s):  
Katherine Milagros Quispe Medina ◽  
◽  
Angel Sixto Mamani Ruelas ◽  
Brenda Jasmin Alvarez Vera ◽  
Yasmin Yessenia Silvestre Gutierrez ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Gastrointestinal Tract ◽  
Molecular Biology ◽  
Gut Microbiome ◽  
Next Generation ◽  
Multiple Factors ◽  
Lung Microbiome ◽  
The Relationship ◽  
Generation Sequencing ◽  
Clinical Cases

The research of the microbiome concerning various diseases has grown in the last ten years due to the advances in molecular biology and next-generation sequencing, finding interactions with various pathologies. The new coronavirus 19 (SARS-COV 2) pandemic has aroused interest in the study of multiple factors that could influence in the development of symptoms mainly due to the interaction of the microbiome whether of the respiratory or gastrointestinal tract finally in the prognosis. Therefore, in this study, we focus on reviewing and analyzing the current bibliography of research and clinical cases about the relationship between the lung and gut microbiome and COVID-19, highlighting its effect on infected patients, aiming to contribute to this new line of research. Keywords: Microbiome, COVID-19, SARS-COV 2, gut microbiome, lung microbiome.

scholarly journals Advances and Limitations of Next Generation Sequencing in Animal Diet Analysis

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1854
Author(s):  
Gang Liu ◽  
Shumiao Zhang ◽  
Xinsheng Zhao ◽  
Chao Li ◽  
Minghao Gong
Keyword(s):  
Next Generation Sequencing ◽  
Environmental Changes ◽  
Diet Analysis ◽  
Next Generation ◽  
Application Range ◽  
Promising Tool ◽  
Composition And Structure ◽  
Next Generation Sequencing Ngs ◽  
Animal Diet ◽  
Generation Sequencing

Diet analysis is a critical content of animal ecology and the diet analysis methods have been constantly improving and updating. Contrary to traditional methods of high labor intensity and low resolution, the next generation sequencing (NGS) approach has been suggested as a promising tool for dietary studies, which greatly improves the efficiency and broadens the application range. Here we present a framework of adopting NGS and DNA metabarcoding into diet analysis, and discuss the application in aspects of prey taxa composition and structure, intra-specific and inter-specific trophic links, and the effects of animal feeding on environmental changes. Yet, the generation of NGS-based diet data and subsequent analyses and interpretations are still challenging with several factors, making it possible still not as widely used as might be expected. We suggest that NGS-based diet methods must be furthered, analytical pipelines should be developed. More application perspectives, including nutrient geometry, metagenomics and nutrigenomics, need to be incorporated to encourage more ecologists to infer novel insights on they work.

Impact of Diet and the Gut Microbiome on Neurodegeneration and Regeneration in Neurological Disorders

Neuroforum ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 39-47
Author(s):  
Barbara Gisevius ◽  
Aiden Haghikia ◽  
Sarah Hirschberg
Keyword(s):  
Next Generation Sequencing ◽  
Gut Microbiome ◽  
Neurological Disorders ◽  
Next Generation ◽  
Generation Sequencing

Zusammenfassung Aktuelle Forschungsergebnisse im Bereich neurodegenerativer Erkrankungen deuten vermehrt darauf hin, dass die Ernährung und damit assoziiert die Zusammensetzung des Darm-Mikrobioms einen entscheidenden Einfluss auf die Entstehung und den Verlauf verschiedenster Krankheiten haben. Die sogenannte Darm-Hirn Achse, oder präziser die Darm-Mikrobiom-Hirn Achse hat dadurch deutlich an Aufmerksamkeit gewonnen. Dabei kann der Darm das zentrale Nervensystem auf unterschiedliche Weisen beeinflussen, I) direkt durch bakterielle Bestandteile und Metaboliten von Bakterien, II) durch Manipulation der im Körper zirkulierenden Immunzellen, oder III) durch direkten Kontakt, z. B. über den N. vagus. Fortschritte auf dem Gebiet der Molekularbiologie, wie das Next Generation Sequencing ermöglichen aufgrund ihres hohen Auflösungsvermögens die genaue Identifikation von Bakterien und die Kompositionen ganzer Mikrobiome. Dadurch ist es möglich, die Interaktionen zwischen dem intestinalen Mikrobiom, dem Metabolom und dem Darm- assoziierten Immunsystem detailliert zu erforschen. In dieser Arbeit diskutieren wir den Einfluss des Mikrobioms, der Ernährung und den damit verbundenen Gesundheitszustand auf die Neuroregeneration. Der Fokus liegt dabei auf der Möglichkeit, wie dieses Wissen in Zukunft für therapeutische Zwecke genutzt werden kann.

scholarly journals Next generation sequencing (NGS) as a multipurpose method for detection and differentiation of plant viruses

2021 ◽  
Vol 61 (4) ◽  
pp. 269-277
Keyword(s):  
Next Generation Sequencing ◽  
Molecular Mechanisms ◽  
Environmental Changes ◽  
Simultaneous Detection ◽  
Disease Outbreaks ◽  
Plant Viruses ◽  
Next Generation ◽  
Early Detection Of Disease ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

This paper describes the application of next generation sequencing (NGS) in plant virus research. Although NGS has not been routinely used yet, it is increasingly adopted in diagnostics and genomics of phytopathogens. NGS technics enable the simultaneous detection of multiple viruses present in infected material. This makes it possible not only to determine which viruses are present in a single sample but also to determine their concentration and genetic diversity. The simultaneous identification of many viruses, the possibility of early detection of disease outbreaks as well as tracking and monitoring of epidemic development, make NGS a universal research tool that enables not only the detection but also the understanding of molecular mechanisms allowing viruses to adapt to environmental changes (host plant genotype, vector, presence of other pathogens).

Sign in / Sign up

Export Citation Format

Share Document