Generalized Recalcitrant Pruritus as the Presenting Manifestation of Hypereosinophilic Syndrome

2016 ◽  
Vol 20 (4) ◽  
pp. 346-348
Author(s):  
Ariane Schreiber ◽  
Martin LeBlanc ◽  
Marie-Marthe Thibeault ◽  
Steve Mathieu
Keyword(s):  
Polymerase Chain Reaction ◽  
Pulmonary Embolism ◽  
Differential Diagnosis ◽  
Hypereosinophilic Syndrome ◽  
Massive Pulmonary Embolism ◽  
Frequent Occurrence ◽  
Diagnosis And Treatment ◽  
Chain Reaction ◽  
Clonal Rearrangement ◽  
Polymerase Chain

Background and Objective: Pruritus is a frequent occurrence in dermatology, and investigation is often unrevealing. The authors report the case of a 65-year-old man presenting with generalized recalcitrant pruritus as the presenting manifestation of hypereosinophilic syndrome. Methods and Results: A 65-year-old man developed intractable pruritus. Results of polymerase chain reaction clonal rearrangement were positive and led to the diagnosis and treatment. The patient also developed massive pulmonary embolism, which can be caused by chronic eosinophilia. Conclusions: This case highlights the importance of investigating patients with pruritus and unexplained persistent eosinophilia. Hypereosinophilic syndrome must be included in the differential diagnosis, which in this case presented initially as intractable pruritus. Polymerase chain reaction clonal rearrangement was key in reinforcing the diagnosis.

Polymerase chain reaction in the diagnosis of parotid gland tuberculosis

1998 ◽  
Vol 112 (5) ◽  
pp. 494-496 ◽  
Author(s):  
Enis Alpin Güneri ◽  
Ahmet Ömer İkiz ◽  
Nese Atabey ◽  
Özlem İzci ◽  
Semih Sütay
Keyword(s):  
Polymerase Chain Reaction ◽  
Differential Diagnosis ◽  
Parotid Gland ◽  
Mycobacterial Infection ◽  
Social Characteristics ◽  
Causative Organism ◽  
Chain Reaction ◽  
First Case ◽  
Polymerase Chain ◽  
High Degree

AbstractA parotid gland mass with presenting features of malignancy is a diagnostic and therapeutic challenge. The histological nature of the lesion must be clearly determined before proceeding with facial nerve sacrificing surgery. Although rare, tuberculosis of the parotid gland must be included in the differential diagnosis of a parotid gland mass especially when the social characteristics of the patient suggests a mycobacterial infection. Primary tuberculosis of the parotid gland is generally encountered among populations with a high incidence of pulmonary disease. The difficulty in the differential diagnosis of a parotid gland malignancy may be helped by a high degree of clinical suspicion, since laboratory tests generally do not identify the specific causative organism. This article reports the first case of parotid gland tuberculosis with clinical and radiodiagnostical features simulating malignancy in which the diagnosis was confirmed by the polymerase chain reaction (PCR).

scholarly journals Nonsuppurative Meningoencephalitis Associated with Aleutian Mink Disease Parvovirus Infection in Ranch Mink

2000 ◽  
Vol 12 (2) ◽  
pp. 159-162 ◽  
Author(s):  
N. W. Dyer ◽  
B. Ching ◽  
M. E. Bloom
Keyword(s):  
Polymerase Chain Reaction ◽  
Nervous System ◽  
Differential Diagnosis ◽  
Viral Genome ◽  
Brain Lesions ◽  
Chain Reaction ◽  
Mesenteric Lymph ◽  
Neurologic Disorders ◽  
Polymerase Chain ◽  
Ranch Mink

Severe nonsuppurative meningoencephalitis associated with Aleutian mink disease parvovirus (ADV) infection was observed in adult ranch mink. Brain lesions included severe, locally extensive to coalescing lymphoplasmacytic meningoencephalitis with accompanying gliosis, satellitosis, and mild extension of inflammation into the leptomeninges. ADV was identified in mesenteric lymph node, spleen, brain, and liver of affected mink by polymerase chain reaction techniques. Sequences of the ADV isolate (TH5) revealed 2 unique residues in the region of the viral genome that determines pathogenicity. These findings suggest that certain strains of ADV may preferentially cause disease in the nervous system. ADV infection should be considered in the differential diagnosis of neurologic disorders in mink.

scholarly journals Efficacy of rapid multiplex polymerase chain reaction for early diagnosis and treatment of pertussis

2019 ◽  
Author(s):  
Se Chang Oh ◽  
Soo Min Park ◽  
Jian Hur ◽  
Eun Young Choi ◽  
Hyun Jung Jin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Diagnosis ◽  
Multiplex Pcr ◽  
Bordetella Pertussis ◽  
Turnaround Time ◽  
University Hospital ◽  
Diagnosis And Treatment ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Pcr Test

Abstract Background Pertussis, a highly infectious respiratory disease caused by Bordetella pertussis, causes airway inflammation and severe, persistent (lasting 2 weeks or more) characteristic whooping cough. In severe cases, complications such as atelectasis and bronchopneumonia may occur. Recently, the prevalence of pertussis has increased in South Korea due to reduced effect of the DTaP vaccination in infants as their age increased. Although culture is the gold standard test for diagnosis, polymerase chain reaction (PCR) method is most commonly used for diagnosis of pertussis due to the low sensitivity and long turnaround time of the culture method. Recently, a rapid multiplex PCR test has been introduced for comprehensive detection of respiratory pathogens (17 viruses and 3 bacteria), including Bordetella pertussis, with a turnaround time of 1 hour. This study aimed to investigate the efficacy of multiplex PCR for early diagnosis and treatment of pertussis. Methods We performed a retrospective study on patients with pertussis diagnosed from May 2017 to June 2019 at Yeungnam University Hospital. Nasopharyngeal swab specimens were tested using multiplex PCR. Medical records collected included data on age, sex, symptoms at the time of diagnosis, admission, hospitalization, isolation, vaccination history, past medical history, and accompanying diseases. Results A total of 27 patients were diagnosed with pertussis, nine (33.3%) of whom were men, with a median age of 48.9 years (3.3–82.2). Eleven (40.7%) had fever, 12 (44.4%) had dyspnea, 3 (11.1%) had paroxysmal cough, and 9 (33.3%) had inspiratory whooping. Seventeen (62.9%) and 24 (88.8%) patients had coughing for <8 days and ≤14 days, respectively. Median time from first symptom to diagnosis was 9.0 (1–31) days. Twenty-four patients (81.5%) were diagnosed within 2 weeks. All but one patient was prescribed macrolide antibiotics; all patients were isolated, with 22 (81.5%) requiring hospitalization. Three patients (11.1%) received ICU care for ventilation. All patients survived. Conclusion A rapid multiplex PCR test can ensure early diagnosis, isolation, and treatment of pertussis. Testing of patients with respiratory symptom with multiplex PCR can led to early diagnosis of pertussis, proper treatment, and may help in outbreak control.

A clinical study to evaluate predictive significance of miR-513b-5p for senile cataract using nanoparticle-assisted polymerase chain reaction method

2021 ◽  
Vol 11 (8) ◽  
pp. 1387-1393
Author(s):  
Ran Sun ◽  
Dachuan Liu ◽  
Minghua Wang
Keyword(s):  
Polymerase Chain Reaction ◽  
Post Treatment ◽  
Diagnosis And Treatment ◽  
Inflammatory Factors ◽  
Eye Lens ◽  
Chain Reaction ◽  
Diagnostic Significance ◽  
Aberrant Expression ◽  
Polymerase Chain ◽  
The Impact

In the past few decades, increase in the global population and improvement in the overall life expectancy has been associated with an increase in the prevalence of age-related cataract. Cataracts (CA) refer to the opacification of the eye lens. It is one of the prime reasons contributing to visual impairment and blindness worldwide. The early onset of CA is relatively insidious, which stands as a major obstacle in the clinical diagnosis and treatment of the disease. Previous studies have reported an aberrant expression of miR-513b-5p in the lens of the eye. Since the eye lens holds a central position in CA, the present study aimed to explore and establish the role of miR-513b-5p in clinical implications of CA using a nanoparticle-assisted polymerase chain reaction (nano-PCR) technology. The study involved two broad groups, 108 elderly CA patients (research group, or RG) and 96 normal physical examinees (control group, or CG). Nano-PCR detection method was used to examine the expression of miR-513b-5p in the two groups. An elevated expression of miR-513b-5p was recorded in RG, which declined after the treatment. ROC curve analysis of the collected data revealed that miR-513b-5p had a favorable diagnostic significance for CA. Post treatment, a positive relevance of miR-513b-5p with inflammatory factors including C-reactive protein (CRP), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) was verified by correlation analysis. The impact of miR-513b-5p on the prognosis of CA patients further established the high predictive significance of miR-513b-5p with regard to the recurrence of CA. Thus, the results of the study showed that miR-513b-5p was highly expressed in senile CA patients and it displayed a superior diagnostic significance with regard to CA. Post treatment, miR-513b-5p expression was found to be strongly linked to the expression of various inflammatory factors. In addition to these, miR-513b-5p displayed an outstanding predictive significance for the recurrence of CA. All these results highlight the suitability of miR-513b-5p to be used as a promising candidate for the diagnosis and treatment of senile CA in the future.

scholarly journals Ocular Tuberculosis with Progressive Unilateral Corneal Melting

2015 ◽  
Vol 6 (3) ◽  
pp. 293-297 ◽  
Author(s):  
Betül N. Bayraktutar ◽  
Ömür Uçakhan-Gündüz
Keyword(s):  
Polymerase Chain Reaction ◽  
Differential Diagnosis ◽  
Case Report ◽  
Biopsy Sample ◽  
Chain Reaction ◽  
Biopsy Material ◽  
Ocular Tuberculosis ◽  
Polymerase Chain ◽  
Corneal Melting ◽  
Peripheral Cornea

Objectives: In this case report, we present a patient with ocular tuberculosis presenting with progressive unilateral corneal melting. Patient: A 47-year-old female was admitted with melting at the inferior half of the peripheral cornea and inferior subconjunctival nodules. Biopsy material of the nodules was negative for tuberculosis bacillus. However, polymerase chain reaction of the biopsy sample revealed the DNA of the bacillus, and the diagnosis was confirmed. Conclusion: In endemic areas, ocular tuberculosis should be kept in mind in the differential diagnosis of patients with chronic and atypical corneal involvement.

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