Epidermal Growth Factor and Tocotrienol-Rich Fraction Cream Formulation Accelerates Burn Healing Process Based on Its Gene Expression Pattern in Deep Partial-Thickness Burn Wound Model

2020 ◽  
pp. 153473462097106
Author(s):  
Hui-Fang Guo ◽  
Razana Mohd Ali ◽  
Roslida Abd Hamid ◽  
Sui Kiat Chang ◽  
Mohammed Habibur Rahman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Healing Process ◽  
Burn Wound ◽  
Expression Levels ◽  
Partial Thickness Burn ◽  
Epidermal Growth ◽  
Tocotrienol Rich Fraction

Our previous study has demonstrated that epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream formulation accelerating postburn wound healing with deep partial-thickness burn in rats. Current study was conducted to determine the gene expression levels related to burn wound healing process. A total of 180 Sprague-Dawley rats were randomly divided into 6 groups: untreated control, treated with Silverdin cream, base cream, base cream with 0.00075% EGF, base cream with 3% TRF or base cream with 0.00075% EGF, and 3% TRF, respectively. Burn wounds were created and the above-mentioned creams were applied once daily. Six animals from each group were sacrificed on days 3, 7, 11, 14, and 21 postburn. RNA was extracted from wound tissues and quantitative real-time polymerase chain reaction was performed to analyze the 9 wound healing-related genes against time postburn. Results demonstrated that topically applied EGF + TRF formulation downregulated the expression levels of IL-6 ( interluekin-6), TNF-α ( tumor necrosis factor-α) and iNOS ( inducible nitric oxide synthase) throughout the whole healing process. TGF-β1 ( transforming growth factor-β) and VEGF-A ( vascular endothelial growth factor-A) were reduced on day 14 postburn. On the contrary, increased expression of Collagen-1 in the early stage of wound healing was observed with no effects on epidemal growth factor receptor (EGFR). The results showed beneficial application of EGF + TRF cream in the treatment of burn wound since it accelerated wound healing by relieving oxidative stress, decreasing inflammation, and promoting proper tissue modelling in the burn wound.

scholarly journals Healing Properties of Epidermal Growth Factor and Tocotrienol-Rich Fraction in Deep Partial-Thickness Experimental Burn Wounds

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 130 ◽  
Author(s):  
Hui-fang Guo ◽  
Roslida Abd Hamid ◽  
Razana Mohd Ali ◽  
Sui Kiat Chang ◽  
Mohammed Habibur Rahman ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Healing Process ◽  
Wound Healing Process ◽  
Partial Thickness ◽  
Partial Thickness Burn ◽  
Nitrite Production ◽  
Microscopic Evaluation ◽  
Epidermal Growth ◽  
Tocotrienol Rich Fraction

Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats were randomly divided into six groups of six each and were: untreated control, treated with Silverdin® cream, base cream, base cream with c% EGF, base cream with 3% TRF or base cream with c% EGF and 3% TRF, respectively. Creams were applied once daily for 21 consecutive days. Six animals from each group were sacrificed using anaesthetic overdose on the third, seventh, 11th, 14th and 21st day post-burn. Skin tissues with the wound to be examined were excised for macroscopic and microscopic evaluation and biochemical analyses. Results: EGF + TRF formulation decreased the number of neutrophils, lymphocytes and myofibroblasts post-burn. However, no effects on the number of adipose cells in the healing process were recorded. In addition, lipid peroxidation and nitrite production were found to be reduced post-burn, reducing oxidative stress. Conclusions: Results of the present study indicate that the addition of EGF with TRF have ameliorating effects on deep-partial thickness burn healing parameters.

An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor

Burns ◽  
2006 ◽  
Vol 32 (3) ◽  
pp. 319-327 ◽  
Author(s):  
Ceren Alemdaroğlu ◽  
Zelihagül Değim ◽  
Nevin Çelebi ◽  
Fatih Zor ◽  
Serdar Öztürk ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Epidermal Growth ◽  
Chitosan Gel

Investigation of epidermal growth factor containing liposome formulation effects on burn wound healing

2008 ◽  
Vol 85A (1) ◽  
pp. 271-283 ◽  
Author(s):  
Ceren Alemdaroğlu ◽  
Zelihagül Degim ◽  
Nevin Celebi ◽  
Mustafa Şengezer ◽  
Melih Alömeroglu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Liposome Formulation ◽  
Epidermal Growth

scholarly journals Evaluation of chitosan gel containing liposome-loaded epidermal growth factor on burn wound healing

2011 ◽  
Vol 8 (4) ◽  
pp. 343-354 ◽  
Author(s):  
Zelihagül Değim ◽  
Nevin Çelebi ◽  
Ceren Alemdaroğlu ◽  
Mustafa Deveci ◽  
Serdar Öztürk ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Epidermal Growth ◽  
Chitosan Gel

scholarly journals Study Literature Peran Epidermal Growth Factor dalam Proses Penyembuhan Luka

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Nur Febrianti ◽  
Takdir Tahir ◽  
Saldy Yusuf
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Egf Receptor ◽  
Normal Skin ◽  
Healing Process ◽  
The Body ◽  
Wound Healing Process ◽  
Epithelial Tissue ◽  
Epidermal Growth

Background: Wounds occur when normal skin structures are damaged. Injury events in Indonesia have increased by 8.2% and the highest prevalence in South Sulawesi is 12.8%. One of the growth factors that play a role in the wound healing process is epidermal growth factor (EGF). EGF includes polypeptides that contain 53 amino acids, and EGF is present in all fluids in the body and platelets. This review aims to determine the role of EGF in the process of wound healing. Method: Data collected since 2017 using 8 databases (pubmed, science direct, google schollar) where the literature used is internationally published literature, additional references are taken from the bibliography of all relevant articles, all relevant articles are reviewed and analyzed. Results: EGF has a role in wound healing. EGF increases motility and epithelial cell migration. EGF can stimulate cell growth, proliferation and differentiation by binding to high affinity to the EGF receptor (EGFR) on the cell surface. The goal of EGF healing is most epithelial tissue, fibroblasts, and endothelial cells. EGF can call three important biological actions in tissue repair including cytoprotection, mitogenesis, and migration. Conclusion: EGF plays an important role in the wound healing process, especially in the re-epithelial process. Based on this review, it is suggested that the selection of dressings that are used should support EGF.

Chitosan-Based Composite Nanosilver Gel Application in Nursing Care of Intensive Care Unit Patients with Severe Burns

2020 ◽  
Vol 12 (7) ◽  
pp. 939-945
Author(s):  
Ningning Rong ◽  
Ruyan Yang ◽  
Yongzan Lu
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
High Efficiency ◽  
Tissue Cell ◽  
Petroleum Jelly ◽  
Burn Wound ◽  
Human Epidermal Growth Factor ◽  
Severe Burns ◽  
Epidermal Growth

Burns are the most common clinical disease in daily life. Burn wounds are vulnerable to bacterial infection due to factors such as edema, hypoxia, and ischemia. However, traditional antibacterial supplements do not achieve satisfactory nursing results. Here, we use chitosan as a carrier by introducing silver nanoparticles (AgNPs) and recombinant human epidermal growth factor (rhEGF) together. We have constructed a new type of high moisture retention, high oxygen permeability, high-efficiency chitosan-nanosilver@recombinant human epidermal growth factor (CSAgNPs@ rhEGF) antibacterial hydrogel dressing. The clinical trials showed that the antibacterial effect of CS-AgNPs@rhEGF hydrogel dressings against Pseudomonas aeruginosa (Pa.) and Staphylococcus epidermidis (Se.) was significantly better than that of petroleum jelly and 1% silver sulfadiazine (1%-SSD). In addition, rhEGF promoted tissue cell proliferation and repair. Thus, the CS-AgNPs@rhEGF hydrogel dressing can significantly improve the wound healing rate and healing time in patients with severe burns compared with traditional dressings. Thus, CS-AgNPs@rhEGF hydrogel dressing is expected to provide a new choice for clinical burn patients to prevent wound infection and tissue repair, and has a broad prospect in burn wound healing.

scholarly journals CORNEAL EPITHELIAL WOUND HEALING

1995 ◽  
Vol 03 (04) ◽  
pp. 957-965 ◽  
Author(s):  
PAUL D. DALE ◽  
JONATHAN A. SHERRATT ◽  
PHILIP K. MAINI
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Healing Rate ◽  
Numerical Solutions ◽  
Healing Process ◽  
Analytic Approximation ◽  
Corneal Surface ◽  
Reaction Diffusion Model ◽  
Epidermal Growth

We propose a reaction-diffusion model of the mechanisms involved in the healing of corneal surface wounds. The model focuses on the stimulus for increased mitotic and migratory activity, specifically the role of epidermal growth factor. We determine an analytic approximation for the speed of travelling wave solutions of the model in terms of the parameters and verify the results numerically. By comparing the predicted speed with experimentally measured healing rates, we conclude that serum-derived factors can alone account for the overall features of the healing process, but that the supply of growth factors by the tear film, in the absence of serum-derived factors, is not sufficient to give the observed healing rate. Numerical solutions of the model equations also confirm the importance of both migration and mitosis for effective wound healing. By modifying the model, we obtain an analytic prediction for the healing rate of corneal surface wounds when epidermal growth factor is applied topically to the wound.

HEALING OF INJURIES OF THE ORAL MUCOSA UNDER THE INFLUENCE OF THE USE OF RECOMBINANT EPIDERMAL GROWTH FACTOR

2020 ◽  
pp. 19-22
Author(s):  
Kabaloeva D.V. ◽  
Akkalaev A.B. ◽  
Tskhovrebov A.Ch.
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Oral Mucosa ◽  
Bacterial Colonization ◽  
Healing Process ◽  
Bactericidal Effect ◽  
Important Place ◽  
Surgical Interventions ◽  
Epidermal Growth

An analysis of modern scientific and practical literature leaves no doubt that among the many problems of a dentist, an important place is occupied by the optimization of wound healing of the oral mucosa in case of injuries. Immunodeficiencies of various origins, elderly and senile age, diabetes mellitus, and severe pathology of the cardiovascular system, hypoxic conditions and other somatic diseases reduce the repair potential of the oral mucosa. In addition, all types of metabolism are disrupted, synthesis and humoral transport are reduced, as well as the secretion of growth factors with saliva and their content in the blood and tissue. The healing process of the oral mucosa is slowed down by bacterial colonization of the wound surface, which can increase the area of inflammation. A common method of treating such patients in a dental clinic is the use of agents aimed at regenerative processes in tissues. Along with the positive qualities of modern drugs (good fixation, comfort when used in the oral cavity, wound healing, bactericidal effect on the damaged mucous membrane, reducing pain, accelerating the processes of epithelialization and scar organization), a noticeable weakening of their action in immunodeficient conditions should be noted. Based on this, it is advisable to use recombinant epidermal growth factor (EGFR) in the treatment of traumatic lesions of the oral mucosa, including those caused by the use of orthopedic structures, surgical interventions, especially those accompanied by conditions involving a decrease in the immune status.

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