scholarly journals Study Literature Peran Epidermal Growth Factor dalam Proses Penyembuhan Luka

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Nur Febrianti ◽  
Takdir Tahir ◽  
Saldy Yusuf
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Egf Receptor ◽  
Normal Skin ◽  
Healing Process ◽  
The Body ◽  
Wound Healing Process ◽  
Epithelial Tissue ◽  
Epidermal Growth

Background: Wounds occur when normal skin structures are damaged. Injury events in Indonesia have increased by 8.2% and the highest prevalence in South Sulawesi is 12.8%. One of the growth factors that play a role in the wound healing process is epidermal growth factor (EGF). EGF includes polypeptides that contain 53 amino acids, and EGF is present in all fluids in the body and platelets. This review aims to determine the role of EGF in the process of wound healing. Method: Data collected since 2017 using 8 databases (pubmed, science direct, google schollar) where the literature used is internationally published literature, additional references are taken from the bibliography of all relevant articles, all relevant articles are reviewed and analyzed. Results: EGF has a role in wound healing. EGF increases motility and epithelial cell migration. EGF can stimulate cell growth, proliferation and differentiation by binding to high affinity to the EGF receptor (EGFR) on the cell surface. The goal of EGF healing is most epithelial tissue, fibroblasts, and endothelial cells. EGF can call three important biological actions in tissue repair including cytoprotection, mitogenesis, and migration. Conclusion: EGF plays an important role in the wound healing process, especially in the re-epithelial process. Based on this review, it is suggested that the selection of dressings that are used should support EGF.

scholarly journals Enhanced Wound Healing by Recombinant Escherichia coli Nissle 1917 via Human Epidermal Growth Factor Receptor in Human Intestinal Epithelial Cells: Therapeutic Implication Using Recombinant Probiotics

2011 ◽  
Vol 80 (3) ◽  
pp. 1079-1087 ◽  
Author(s):  
Hye Jin Choi ◽  
Jung Hoon Ahn ◽  
Seong-Hwan Park ◽  
Kee Hun Do ◽  
Juil Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Abc Transporter ◽  
Egf Receptor ◽  
Mitogen Activated Protein Kinase ◽  
Content Type ◽  
E Coli ◽  
Epithelial Migration ◽  
Epidermal Growth

The gastrointestinal mucosa has a remarkable ability to repair damage with the support of epidermal growth factor (EGF), which stimulates epithelial migration and proliferative reepithelialization. For the treatment of mucosal injuries, it is important to develop efficient methods for the localized delivery of mucoactive biotherapeutics. The basic idea in the present study came from the assumption that an intestinal probiotic vehicle can carry and deliver key recombinant medicinal proteins to the injured epithelial target in patients with intestinal ulcerative diseases, including inflammatory bowel disease. The study was focused on the use of the safe probioticE. coliNissle 1917, which was constructed to secrete human EGF in conjunction with the lipase ABC transporter recognition domain (LARD). Using thein vitrophysically wounded monolayer model, ABC transporter-mediated EGF secretion by probioticE. coliNissle 1917 was demonstrated to enhance the wound-healing migration of human enterocytes. Moreover, the epithelial wound closure was dependent on EGF receptor-linked activation, which exclusively involved the subsequent signaling pathway of the mitogen-activated protein kinase kinase (MEK) extracellular-related kinases 1 and 2 (ERK1/2). In particular, the migrating frontier of the wounded edge displayed the strongest EGF receptor-linked signaling activation in the presence of the recombinant probiotic. The present study provides a basis for the clinical application of human recombinant biotherapeutics via an efficient, safe probiotic vehicle.

Role of Topical Biological Therapies and Dressings in Healing Ischemic Wounds

2018 ◽  
Vol 17 (4) ◽  
pp. 236-246
Author(s):  
Saritphat Orrapin ◽  
Kittipan Rekasem
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Platelet Rich Plasma ◽  
Wound Care ◽  
Healing Process ◽  
Platelet Derived Growth Factor ◽  
Wound Healing Process ◽  
Biological Therapies ◽  
Biological Dressing ◽  
Epidermal Growth

Ischemic wounds are the most severe expression of critical limb ischemia (CLI), and they have been defined clinically as an end stage of peripheral arterial disease. Urgent revascularization is a fundamental part for limb salvage in patients with CLI. However, the risk of revascularization should be weighed against the likelihood of success given a patient’s life-threatening comorbidities. Once the condition of arterial insufficiency is revascularized, wound care is an important aspect to promote the wound healing process and infection control. MOIST concept for wound care is a modern systematic treatment for enhanced wound healing process. Currently, advanced biological therapies are emerging in ischemic wound therapies to restore the wound healing process and involve active biological agents to support the wound healing process. We studied and summarized the different types of available topical biological therapies and their mechanisms on the healing process including platelet-derived growth factor, epidermal growth factor, fibroblast growth factor, and vascular endothelial growth factor, platelet-rich plasma, and honey for local wound care of patient with CLI. Our review suggests that topical platelet-derived growth factor, epidermal growth factor, platelet-rich plasma, and honey are available as well as considered in the ischemic wound healing process enhancement through the MOIST concept. In conclusion, biologic wound dressing or topical agent therapy may improve the wound healing process, increase limb salvage, is inexpensive, and provides potential safety with nontoxic low-risk therapy in patients with an ischemic wound. Thus, local wound care by biological dressing should be added in adjuvant treatment for ischemic wound patients. However, further randomized studies are needed to support efficacy and long-term outcomes of these biological dressing in patients with ischemic wound.

scholarly journals CORNEAL EPITHELIAL WOUND HEALING

1995 ◽  
Vol 03 (04) ◽  
pp. 957-965 ◽  
Author(s):  
PAUL D. DALE ◽  
JONATHAN A. SHERRATT ◽  
PHILIP K. MAINI
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Healing Rate ◽  
Numerical Solutions ◽  
Healing Process ◽  
Analytic Approximation ◽  
Corneal Surface ◽  
Reaction Diffusion Model ◽  
Epidermal Growth

We propose a reaction-diffusion model of the mechanisms involved in the healing of corneal surface wounds. The model focuses on the stimulus for increased mitotic and migratory activity, specifically the role of epidermal growth factor. We determine an analytic approximation for the speed of travelling wave solutions of the model in terms of the parameters and verify the results numerically. By comparing the predicted speed with experimentally measured healing rates, we conclude that serum-derived factors can alone account for the overall features of the healing process, but that the supply of growth factors by the tear film, in the absence of serum-derived factors, is not sufficient to give the observed healing rate. Numerical solutions of the model equations also confirm the importance of both migration and mitosis for effective wound healing. By modifying the model, we obtain an analytic prediction for the healing rate of corneal surface wounds when epidermal growth factor is applied topically to the wound.

scholarly journals The activin binding proteins follistatin and follistatin-related protein are differentially regulated in vitro and during cutaneous wound repair

2001 ◽  
Vol 171 (3) ◽  
pp. 385-395 ◽  
Author(s):  
M Wankell ◽  
S Kaesler ◽  
YQ Zhang ◽  
C Florence ◽  
S Werner ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Binding Proteins ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Wound Healing Process ◽  
Epidermal Growth

Follistatin is a secreted protein that binds activin in vitro and in vivo and thereby inhibits its biological functions. Recently, related human and murine genes, designated follistatin-related gene (FLRG), were identified, and their products were shown to bind activin with high affinity. In this study we further characterized the murine FLRG protein, and we analyzed its tissue-specific expression and regulation in comparison with those of follistatin. Transient expression of the mouse FLRG protein in COS-1 cells revealed that the FLRG cDNA encodes a secreted glycoprotein. FLRG mRNA was expressed at high levels in the lung, the testis, the uterus and, particularly, the skin. Immunohistochemistry revealed the presence of FLRG in the basement membrane between the dermis and the epidermis and around blood vessels. FLRG mRNA expression was induced in keratinocytes by keratinocyte growth factor, epidermal growth factor and transforming growth factor-beta 1, and in fibroblasts by platelet-derived growth factor and epidermal growth factor. The induction was more rapid, but weaker, than that of follistatin. Most interestingly, both follistatin and FLRG were expressed during the wound healing process, but their distribution within the wound was different. The different expression pattern of FLRG and follistatin and their differential regulation suggest different functions of these activin-binding proteins in vivo.

Epidermal Growth Factor and Tocotrienol-Rich Fraction Cream Formulation Accelerates Burn Healing Process Based on Its Gene Expression Pattern in Deep Partial-Thickness Burn Wound Model

2020 ◽  
pp. 153473462097106
Author(s):  
Hui-Fang Guo ◽  
Razana Mohd Ali ◽  
Roslida Abd Hamid ◽  
Sui Kiat Chang ◽  
Mohammed Habibur Rahman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Healing Process ◽  
Burn Wound ◽  
Expression Levels ◽  
Partial Thickness Burn ◽  
Epidermal Growth ◽  
Tocotrienol Rich Fraction

Our previous study has demonstrated that epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream formulation accelerating postburn wound healing with deep partial-thickness burn in rats. Current study was conducted to determine the gene expression levels related to burn wound healing process. A total of 180 Sprague-Dawley rats were randomly divided into 6 groups: untreated control, treated with Silverdin cream, base cream, base cream with 0.00075% EGF, base cream with 3% TRF or base cream with 0.00075% EGF, and 3% TRF, respectively. Burn wounds were created and the above-mentioned creams were applied once daily. Six animals from each group were sacrificed on days 3, 7, 11, 14, and 21 postburn. RNA was extracted from wound tissues and quantitative real-time polymerase chain reaction was performed to analyze the 9 wound healing-related genes against time postburn. Results demonstrated that topically applied EGF + TRF formulation downregulated the expression levels of IL-6 ( interluekin-6), TNF-α ( tumor necrosis factor-α) and iNOS ( inducible nitric oxide synthase) throughout the whole healing process. TGF-β1 ( transforming growth factor-β) and VEGF-A ( vascular endothelial growth factor-A) were reduced on day 14 postburn. On the contrary, increased expression of Collagen-1 in the early stage of wound healing was observed with no effects on epidemal growth factor receptor (EGFR). The results showed beneficial application of EGF + TRF cream in the treatment of burn wound since it accelerated wound healing by relieving oxidative stress, decreasing inflammation, and promoting proper tissue modelling in the burn wound.

HEALING OF INJURIES OF THE ORAL MUCOSA UNDER THE INFLUENCE OF THE USE OF RECOMBINANT EPIDERMAL GROWTH FACTOR

2020 ◽  
pp. 19-22
Author(s):  
Kabaloeva D.V. ◽  
Akkalaev A.B. ◽  
Tskhovrebov A.Ch.
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Oral Mucosa ◽  
Bacterial Colonization ◽  
Healing Process ◽  
Bactericidal Effect ◽  
Important Place ◽  
Surgical Interventions ◽  
Epidermal Growth

An analysis of modern scientific and practical literature leaves no doubt that among the many problems of a dentist, an important place is occupied by the optimization of wound healing of the oral mucosa in case of injuries. Immunodeficiencies of various origins, elderly and senile age, diabetes mellitus, and severe pathology of the cardiovascular system, hypoxic conditions and other somatic diseases reduce the repair potential of the oral mucosa. In addition, all types of metabolism are disrupted, synthesis and humoral transport are reduced, as well as the secretion of growth factors with saliva and their content in the blood and tissue. The healing process of the oral mucosa is slowed down by bacterial colonization of the wound surface, which can increase the area of inflammation. A common method of treating such patients in a dental clinic is the use of agents aimed at regenerative processes in tissues. Along with the positive qualities of modern drugs (good fixation, comfort when used in the oral cavity, wound healing, bactericidal effect on the damaged mucous membrane, reducing pain, accelerating the processes of epithelialization and scar organization), a noticeable weakening of their action in immunodeficient conditions should be noted. Based on this, it is advisable to use recombinant epidermal growth factor (EGFR) in the treatment of traumatic lesions of the oral mucosa, including those caused by the use of orthopedic structures, surgical interventions, especially those accompanied by conditions involving a decrease in the immune status.

scholarly journals Healing Properties of Epidermal Growth Factor and Tocotrienol-Rich Fraction in Deep Partial-Thickness Experimental Burn Wounds

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 130 ◽  
Author(s):  
Hui-fang Guo ◽  
Roslida Abd Hamid ◽  
Razana Mohd Ali ◽  
Sui Kiat Chang ◽  
Mohammed Habibur Rahman ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Healing Process ◽  
Wound Healing Process ◽  
Partial Thickness ◽  
Partial Thickness Burn ◽  
Nitrite Production ◽  
Microscopic Evaluation ◽  
Epidermal Growth ◽  
Tocotrienol Rich Fraction

Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats were randomly divided into six groups of six each and were: untreated control, treated with Silverdin® cream, base cream, base cream with c% EGF, base cream with 3% TRF or base cream with c% EGF and 3% TRF, respectively. Creams were applied once daily for 21 consecutive days. Six animals from each group were sacrificed using anaesthetic overdose on the third, seventh, 11th, 14th and 21st day post-burn. Skin tissues with the wound to be examined were excised for macroscopic and microscopic evaluation and biochemical analyses. Results: EGF + TRF formulation decreased the number of neutrophils, lymphocytes and myofibroblasts post-burn. However, no effects on the number of adipose cells in the healing process were recorded. In addition, lipid peroxidation and nitrite production were found to be reduced post-burn, reducing oxidative stress. Conclusions: Results of the present study indicate that the addition of EGF with TRF have ameliorating effects on deep-partial thickness burn healing parameters.

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