Isolation of Antitrypanosomal Compounds from Vitis repens, a Medicinal Plant of Myanmar

Khine Swe Nyunt; Ahmed Elkhateeb; Yusuke Tosa; Kensuke Nabata; Ken Katakura; Hideyuki Matsuura

doi:10.1177/1934578x1200700516

Isolation of Antitrypanosomal Compounds from Vitis repens, a Medicinal Plant of Myanmar

Natural Product Communications ◽

10.1177/1934578x1200700516 ◽

2012 ◽

Vol 7 (5) ◽

pp. 1934578X1200700

Author(s):

Khine Swe Nyunt ◽

Ahmed Elkhateeb ◽

Yusuke Tosa ◽

Kensuke Nabata ◽

Ken Katakura ◽

...

Keyword(s):

Medicinal Plant ◽

Ethanolic Extract ◽

Trypanosoma Evansi ◽

Antitrypanosomal Activity

Bioactivity-guided fractionation of an ethanolic extract of Vitis repens led to the isolation of resveratrol (1), 11- O-acetyl bergenin (2), and stigmast-4-en-3-one (3). The compounds were examined for their in vitro antitrypanosomal activities against trypomastigotes of Trypanosoma evansi. Resveratrol showed antitrypanosomal activity with an IC50 value of 0.13 μM, whereas 11- O-acetyl bergenin and stigmast-4-en-3-one exhibited IC50 values of 0.17 and 0.15 μM, respectively.

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Medicinal plant Potentilla fulgens and its effect in vitro against Fasciola gigantica

Insights in Clinical and Cellular Immunology ◽

10.29328/journal.icci.1001018 ◽

2021 ◽

Vol 5 (1) ◽

pp. 004-007

Author(s):

Kumar Pradeep

Keyword(s):

Medicinal Plant ◽

Liver Fluke ◽

Fasciola Hepatica ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Organic Extract ◽

Lc50 Value ◽

Organic Extracts ◽

Giant Liver Fluke

Fascioliasis is a one of the most important serious parasitic zoonotic disease which caused by trematode giant liver fluke Fasciola hepatica and F. gigantica among cattle’s and humans. The infection of Fasciola can be control by the use of phytochemicals as anthelmintic components. The anthelmintic activities of dried root powder of medicinal plant Potentilla fulgens and their different preparations (organic extracts and column purified fraction) are uses in vitro against liver fluke F. gigantica. The dried root powder, different organic extract, and column fractions were time and concentration-dependent. Among all the organic extracts, ethanol extract was high toxic than other organic extracts. The toxic effect of ethanolic extract of P. fulgens after 2h exposure the LC50 value is 5.22 mg/ml against F. gigantica. The column purified fraction of dried root powder of P. fulgens shows more toxicity. The 2h LC50 of column purified fraction was 3.25 mg/ml whereas in 8h exposure the LC50 is 1.24 mg/ml. The phytochemicals of the P. fulgens may be used as anthelmintic components against liver fluke F. gigantica.

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IN VITRO STUDY OF ANTITRYPANOSOMAL ACTIVITY OF ETHANOLIC LEAF EXTRACT OF GARCINIA KOLA AGAINST TRYPANOSOMA BRUCEI BRUCEI

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i6.25022 ◽

2018 ◽

Vol 11 (6) ◽

pp. 417 ◽

Cited By ~ 1

Author(s):

Ali Sani ◽

Umar Abdullahi Zakariyya ◽

Asmau Mahe ◽

Deepa Singh

Keyword(s):

Trypanosoma Brucei ◽

Cardiac Glycosides ◽

In Vitro Study ◽

Ethanolic Extract ◽

Trypanosoma Brucei Brucei ◽

Plant Materials ◽

Garcinia Kola ◽

Antitrypanosomal Activity ◽

Ethanolic Leaf Extract

Objective: The study was aimed at evaluating in vitro antitrypanosomal activity of Garcinia kola against Trypanosoma brucei brucei.Methods: The plant materials were extracted and screened for phytochemicals. The antitrypanosomal activity of 70% and 100% G. kola extracts against the parasite was determined in vitro.Results: Both extracts contained saponins, tannins, carbohydrates, cardiac glycosides, and flavonoids. However, anthraquinones and alkaloids were undetected. The parasites were seen to be actively motile within the first 30 min post-incubation period in 70% hydroethanolic G. kola extract and were found dead at 40–60 min in a higher concentration of extract. After 10 min post-incubation of the parasites in 100% ethanolic extract of G. kola, the parasites were observed to be active at lower concentration but at higher concentrations of the extracts, no trypanosomes were seen.Conclusion: 100% extract induces ceasing of motility at the lesser time compared to 70% ethanolic extract. Similarly, the effect increases with increase in extract concentration. Further research should be carried out to elucidate the bioactive compounds present to have a broad knowledge on the mode of action of the compounds.

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The in vitro antitrypanosomal activity of Albizia gummifera leaf extracts

Open Veterinary Science ◽

10.1515/ovs-2020-0105 ◽

2021 ◽

Vol 2 (1) ◽

pp. 33-39

Author(s):

Deborah Adebukola Oloruntola ◽

Ebenezer Oluyemi Dada ◽

Muftau Kolawole Oladunmoye

Keyword(s):

Ethanolic Extract ◽

Chemotherapeutic Drugs ◽

Leaf Extracts ◽

Trypanocidal Activity ◽

Antitrypanosomal Activity ◽

Test Concentration ◽

Albizia Gummifera ◽

Parasite Motility

Abstract For the control and treatment of trypanosomiasis, a limited number of chemotherapeutic drugs with mild side effects are available. As a result, a quest for a less toxic herbal treatment for trypanosomiasis is needed. Ethanolic extract of A. gummifera leaf (EEAL) and aqueous extract of Albizia. gummifera leaf (AEAL) were tested for antitrypanosomal activity against Trypanosoma brucei brucei in vitro. We first compared the phytochemical concentrations of EEAL and AEAL and discovered that EEAL had higher phytochemical concentrations on average than AEAL: flavonoids (4.26 mg/g vs 2.50 mg/g); alkaloids (38.40 mg/g vs 19.80 mg/g); tannins (230.7 mg/g vs 45.74 mg/g) and saponins (128.66 vs 44.33g/g). From the result of phytochemical concentrations of the two compounds, the higher values observed in flavonoids and alkaloid of EEAL led us to hypothesize that EEAL would have greater trypanocidal activity. Following that, EEAL and AEAL were tested for antitrypanosomal activity in vitro. Forty µl of blood holding in about 25±8 parasites/field was mixed with 20 µl of the EEAL and AEAL solutions of 100, 80, 60 mg/ml to produce an efficacious test concentration of 25, 20 and 15 mg/ml, sequentially. The extracts inhibited parasite motility and eliminated the organisms at the concentrations used in vitro, except for 15 mg/ml AEAL and 20 mg/ml AEAL. Following the screening, the Albizia gummifera ethanolic extract found to have positive in vitro trypanocidal activity. More research is needed to determine the concentrations of the extract for the in vivo test.

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Screening of six medicinal plant species for antileishmanial activity

Acta Pharmaceutica ◽

10.2478/acph-2021-0023 ◽

2021 ◽

Vol 71 (3) ◽

pp. 399-414

Author(s):

Fabiana Borges Padilha Ferreira ◽

Márcia Regina Pereira Cabral ◽

Maria Helena Sarragiotto ◽

Carla Maria Mariano Fernandez ◽

Zilda Cristiani Gazim ◽

...

Keyword(s):

Medicinal Plant ◽

Plant Species ◽

High Performance ◽

Ethanolic Extract ◽

Medicinal Plant Species ◽

Chemical Components ◽

Leishmania Braziliensis ◽

Ethanolic Extracts ◽

Low Cytotoxicity

AbstractThis study is aimed to investigate the in vitro anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonocarpa and Piper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of Leishmania amazonensis and Leishmania braziliensis and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for L. amazonensis and L. braziliensis were shown by the ethanolic extract of the fruits of Hymenaea stigonocarpa and dichloromethane extract of the roots of Piper corcovadensis, with IC50 of 160 and 150 μg mL−1, resp. Ethanolic extracts of A. muricata (leaf), B. uniflora (flower and leaf), M. pungens (fruit and leaf), N. megapotamica (leaf), and aqueous extract of H. stigonocarpa (fruit) showed IC50 > 170 μg mL−1 for L. amazonensis and > 200 μg mL−1 for L. braziliensis. The extracts exhibited low cytotoxicity towards J774.A1 macrophages with CC50 > 1000 μg mL−1 and hemolytic activity from 0 to 46.1 %.

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