scholarly journals Cytotoxic Evaluation of Compounds Isolated from the Aerial Parts of Hedyotis pilulifera and Methanol Extract of Inonotus obliquus

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Hoai Thi Nguyen ◽  
Duc Viet Ho ◽  
Phu Dinh Quynh Nguyen ◽  
Hung Quoc Vo ◽  
Thao Thi Do ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Cancer Cell Lines ◽  
Hep G2 ◽  
Inonotus Obliquus ◽  
Cytotoxic Effects ◽  
First Report ◽  
Aerial Parts

The aim of this study was to evaluate the inhibitory activity of compounds isolated from the aerial parts of Hedyotis pilulifera (Pit.) T.N. Ninh toward selected cancer cell lines. The isolated compounds were identified by analyzing their nuclear magnetic resonance spectral data and physical properties, and comparison of these with reported data. The sulforhodamine B assay was used for the cytotoxic evaluation of isolates. Among twenty-one compounds isolated from H. pilulifera, compounds 2, 3, and 4 showed moderate inhibitory effect on MCF-7 with IC50 values of 63.5, 59.4, and 52.7 μg/mL, respectively, while the other compounds exhibited no effect (IC50 values > 100 μg/mL). Further investigation using HT29, LU-1, HL-60, KB, Hep G2, and SK-Mel2 cancer cell lines showed the moderate cytotoxic activity of compound 3 (IC50 values ranging from 51.7 to 78.3 μg/mL) to all cells, while compound 4 showed selective effect only against HL-60 cells (IC50 61.5 μg/mL). This is the first report of cytotoxic activity of pomolic acid 3β-acetate (3) toward all tested cancer cell lines, and also the first report of cytotoxicity of rotungenic acid (4) against LU-1, HL-60, KB, Hep G2, and SK-Mel2 cancer cell lines. The methanol extract of chaga mushroom { Inonotus obliquus (Ach. ex Pers.) Pilát} exhibited the strongest cytotoxic effects against HL-60 and LU-1 (32.2 and 38.0 μg/mL, respectively), and modest cytotoxic effects against SW480 (41.3 μg/mL), HepG2 (51.3 μg/mL), KB (57.0 μg/mL), and LNCaP (57.7 μg/mL). We conclude that compounds 3 and 4 from H. pilulifera may be useful in further investigation for anticancer agent discovery and chaga could be used as a natural anticancer remedy against promyelocytic leukemia and lung adenocarcinoma.

scholarly journals Cytotoxic Alkaloids from Leaves and Twigs of Dasymaschalon sootepense

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Sakchai Hongthong ◽  
Chutima Kuhakarn ◽  
Vichai Reutrakul ◽  
Surawat Jariyawat ◽  
Pawinee Piyachaturawat ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Cancer Cell Lines ◽  
Spectroscopic Methods ◽  
Aporphine Alkaloid ◽  
First Report ◽  
Bioassay Guided Fractionation

Bioassay-guided fractionation of the cytotoxic ethyl acetate fraction of the sequential methanol extract from the leaves and twigs of Dasymaschalon sootepense led to the isolation of a new 7-hydroxy aporphine alkaloid, 6a,7-dehydrodasymachaline (1) along with the five known compounds (-)-nordicentrine (2), dicentrinone (3), (-)-sinactine (4), aristolactam AII (5) and epiberberine (6). Their structures were elucidated by spectroscopic methods. This is the first report of alkaloids 1–2 and 5–6 from the genus Dasymaschalon. Compounds 1 and 5 showed cytotoxicity against a panel of cancer cell lines.

scholarly journals SYNTHESIS AND CYTOTOXIC ACTIVITY EVALUATION OF NOVEL DERIVATIVES OF MURRAYAFOLINE A

2018 ◽  
Vol 54 (2C) ◽  
pp. 502
Author(s):  
Le Duc Anh
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Hep G2 ◽  
Human Cancer Cell Lines ◽  
Activity Evaluation ◽  
Derivatives Of

Two new conjugates of murrayafoline A with zerumbone and artemisinin 3, 11 wereprepared by N-alkylation, in which, compound 3 was synthesized from two consecutive Nalkylationreactions. Their cytotoxicity was evaluated on four human cancer cell lines Hep-G2,LU, RD and Fl. The result showed that both compounds exhibited no activity against the testedcell lines.

scholarly journals A New Metabolite from the Endophytic Fungus Penicillium citrinum

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Xinlan Li ◽  
Liang Zhang ◽  
Yanhui Liu ◽  
Zhiyong Guo ◽  
Zhangshuang Deng ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Endophytic Fungus ◽  
Cancer Cell Lines ◽  
Penicillium Citrinum ◽  
Hep G2 ◽  
Different Levels

A new polyketide, penicillocitrin A (1), together with four known compounds, was isolated from the endophytic fungus Penicillium citrinum (CTGU-TS-24) of Tapiscia sinensis Oliv., and their structures were elucidated by NMR spectroscopic and MS spectrums. The five compounds were evaluated cytotoxic activity to four cancer cell lines A549, Hep G2, Hela and Caski at different levels.

Anticancer Cardenolides from the aerial parts of Calortopis procera

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Nuha Sweidan ◽  
Ezaldeen Esawi ◽  
Mohammad Ismail ◽  
Walhan Alshaer
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Wide Spectrum ◽  
Cancer Cell Line ◽  
Cancer Cell Lines ◽  
Flavonoid Glycoside ◽  
Methanolic Extracts ◽  
Aerial Parts

Abstract Column chromatography (CC) analysis of methanol and butanol extracts of the aerial parts of Calortopis procera as well as the methanol extract of its latex, led to the isolation of 8 cardenolides, of which the structures were elucidated by NMR and HRESIMS spectroscopy. They also revealed several triterpenes and flavonoid glycoside. Based on the antiproliferative activity reported for cardenolides, the activity of calotropin and calotoxin was tested against two common cancer cell lines, human triple-negative breast cancer cell line (MDA-MB-231) and human lung adenocarcinoma cell line (A549). The high toxicity of the latex also encouraged performing the same test on the same cancer cell lines. The anti-proliferative activity of calotropin and calotoxin was compared to the methanol extract and the wax of the latex. The results showed that calotropin and calotoxin have significant cytotoxicity against MDA-MB-231 and A549 cell lines ranging from 0.046 to 0.072 μM compared to the methanol extract and the wax of its latex ranging from 0.47 to 58.41 μM. Moreover, the results showed lower toxicity of all treatments to the human skin fibroblasts compared to the toxicity to both MDA-MB-231 and A549 cancer cells lines except the higher toxicity of Methanolic extracts of C. procera latex to the MDA-MB-231 cells. In conclusion, C. procera is a medicinal plant with a wide spectrum of cardinolides including calotropin and calotoxin, which are promising agents for targeted cancer phytotherapy.

scholarly journals Synthesis and In Vitro Cytotoxic Activity of Chromenopyridones

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Balwinder Singh ◽  
Vishal Sharma ◽  
Gagandeep Singh ◽  
Rakesh Kumar ◽  
Saroj Arora ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Hep G2 ◽  
Cytotoxic Potential ◽  
Human Cancer Cell Lines ◽  
Mcf 7

Novel substituted chromenopyridones (3a–j and 6a–d) were synthesized and evaluated in vitro for the cytotoxic activity against various human cancer cell lines such as prostate (PC-3), breast (MCF-7), CNS (IMR-32), cervix (Hela), and liver (Hep-G2). preliminary cytotoxic screening showed that all the compounds possess a good to moderate inhibitory activity against various cancer cell lines. Particularly, compound 6b bearing allyl moiety displayed a significant cytotoxic potential in comparison to standard drugs.

scholarly journals Cytotoxic activity of casearborin c isolated from Casearia corymbosa

2019 ◽  
Vol 62 (3) ◽  
Author(s):  
María Leonor Vila-Luna ◽  
Rosa Esther Moo-Puc ◽  
Luis Wiliunfo Torres-Tapia ◽  
Sergio Rubén Peraza-Sánchez
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Stem Bark ◽  
Cancer Cell Lines ◽  
Syringic Acid

<p><strong>Abstract. </strong>Casearborin c (<strong>1</strong>), syringic acid (<strong>2</strong>), <em>ent</em>-3β-hydroxy-(-)-13-<em>epi</em>-manoyl oxide (<strong>3</strong>), <em>ent</em>-(-)-13-<em>epi</em>-manoyl oxide (<strong>4</strong>), <em>ent</em>-(-)-kaur-16-en-19-oic acid (<strong>5</strong>), and γ-sitosterol (<strong>6</strong>) were isolated from <em>Casearia corymbosa </em>stem bark. Only casearborin c showed cytotoxic activity on HeLa and SiHa cancer cell lines. This work contributes to the description of three compounds (<strong>1</strong>-<strong>3</strong>) newly isolated from <em>C. corymbosa </em>and highlights that casearborin c, a clerodane-type diterpene, is responsible for the cytotoxic activity shown in the original methanol extract of this species.</p><p> </p><p><strong>Resumen. </strong>Casearborina c (<strong>1</strong>), ácido siríngico (<strong>2</strong>), óxido de <em>ent</em>-3β-hidroxi-(-)-13-<em>epi</em>-manoilo (<strong>3</strong>), óxido de <em>ent</em>-(-)-13-<em>epi</em>-manoilo (<strong>4</strong>), ácido <em>ent</em>-(-)-kaur-16-en-19-oico (<strong>5</strong>) y γ-sitosterol (<strong>6</strong>) se aislaron de la corteza del tallo de <em>Casearia corymbosa</em>. Sólo casearborina c mostró actividad citotóxica en las líneas de células cancerígenas HeLa y SiHa. Este trabajo describe tres compuestos (<strong>1</strong>-<strong>3</strong>) aislados por primera vez de <em>C. corymbosa</em> y destaca que casearborina c, un diterpeno de tipo clerodano, es responsable de la actividad citotóxica del extracto metanólico de esta especie.</p>

scholarly journals Tonantzitlolone A and other cytotoxic constituents of Sapium macrocarpum (Euphorbiaceae)

2017 ◽  
Vol 61 (1) ◽  
Author(s):  
Fernando Novillo ◽  
Verónica Rosero ◽  
María Isabel Chávez ◽  
Simón Hernández-Ortega ◽  
Esteban M. Martínez ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Skin Diseases ◽  
Chemical Constituents ◽  
Cancer Cell Lines ◽  
Additional Source ◽  
H Nmr ◽  
Aerial Parts ◽  
The Family

<p><em>Sapium macrocarpum</em> (Euphorbiaceae) is a tropical tree located mainly in the southern part of Mexico and in Central America, and it is used in the Mayan traditional medicine for the treatment of several skin diseases. The dichloromethane-MeOH extract of the aerial parts displayed cytotoxic activity against certain cancer cell lines. Lupeol (<strong>1</strong>), lupenone (<strong>2</strong>), sitostenone (<strong>3</strong>), β-sitosterol (<strong>4</strong>), stigmasterol (<strong>5</strong>), sitosteryl β-D-glucopyranoside (<strong>6</strong>) and the rare diterpene tonantzitlolone A (<strong>7</strong>) were characterized from this extract. The structural analysis allowed the <sup>1</sup>H NMR reassignments for H-12α and H-12β, and for H-13α and H-13β of tonantzitlolone A (<strong>7</strong>). The cytotoxic activity of compounds <strong>1-3</strong>, <strong>6</strong> and <strong>7</strong> was evaluated against selected cancer cell lines. Compounds <strong>3</strong>, <strong>6</strong> and <strong>7</strong> displayed clear activity against K562 (leukemia). This is the first report on the chemical constituents of <em>S. macrocarpum</em>, and points out that this species is an additional source of tonantzitlolone A. Compound <strong>7</strong> has only been reported previously from species of the <em>Stillingia</em>, <em>Sebastiania</em> and <em>Sapium genera</em>, which belong to the Hippomaninae subtribe of the family Euphorbiaceae.</p>

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