scholarly journals Cytotoxic Alkaloids from Leaves and Twigs of Dasymaschalon sootepense

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Sakchai Hongthong ◽  
Chutima Kuhakarn ◽  
Vichai Reutrakul ◽  
Surawat Jariyawat ◽  
Pawinee Piyachaturawat ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Cancer Cell Lines ◽  
Spectroscopic Methods ◽  
Aporphine Alkaloid ◽  
First Report ◽  
Bioassay Guided Fractionation

Bioassay-guided fractionation of the cytotoxic ethyl acetate fraction of the sequential methanol extract from the leaves and twigs of Dasymaschalon sootepense led to the isolation of a new 7-hydroxy aporphine alkaloid, 6a,7-dehydrodasymachaline (1) along with the five known compounds (-)-nordicentrine (2), dicentrinone (3), (-)-sinactine (4), aristolactam AII (5) and epiberberine (6). Their structures were elucidated by spectroscopic methods. This is the first report of alkaloids 1–2 and 5–6 from the genus Dasymaschalon. Compounds 1 and 5 showed cytotoxicity against a panel of cancer cell lines.

scholarly journals Cytotoxic and Anti-HIV Phenanthroindolizidine Alkaloids from Cryptocarya chinensis†

2012 ◽  
Vol 7 (6) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Tian-Shung Wu ◽  
Chung-Ren Su ◽  
Kuo-Hsiung Lee
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Optical Rotation ◽  
Ethanol Extract ◽  
Cancer Cell Lines ◽  
Spectroscopic Methods ◽  
Bioassay Guided Fractionation ◽  
Phenanthroindolizidine Alkaloids ◽  
Anti Hiv

Bioassay-guided fractionation of the cytotoxic ethanol extract of Cryptocarya chinensis has led to the isolation of 11 compounds, including two phenanthroindolizidine alkaloids [(-)-antofine (1) and dehydroantofine (2)], five pavine alkaloids (3-7), and four proaporphine alkaloids (8-11). The structures of the isolated compounds were determined by means of NMR spectroscopic methods, and supported by HRMS and optical rotation data. Compounds 1 and 2 showed cytotoxic activity against four cancer cell lines, L1210, P388, A549, and HCT-8, with 1 being the most potent against A549 and HCT-8 with EC50 values of 0.002 and 0.001 μg/mL, respectively. In addition, 2 is first reported to exhibit significant anti-HIV activity.

scholarly journals Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4009
Author(s):  
Ahmed S. Abu Zaid ◽  
Ahmed E. Aleissawy ◽  
Ibrahim S. Yahia ◽  
Mahmoud A. Yassien ◽  
Nadia A. Hassouna ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Streptomyces Griseus ◽  
Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Chemical Structures ◽  
Bioassay Guided Fractionation

Background: This study aimed to produce, purify, structurally elucidate, and explore the biological activities of metabolites produced by Streptomyces (S.) griseus isolate KJ623766, a recovered soil bacterium previously screened in our lab that showed promising cytotoxic activities against various cancer cell lines. Methods: Production of cytotoxic metabolites from S. griseus isolate KJ623766 was carried out in a 14L laboratory fermenter under specified optimum conditions. Using a 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide assay, the cytotoxic activity of the ethyl acetate extract against Caco2 and Hela cancer cell lines was determined. Bioassay-guided fractionation of the ethyl acetate extract using different chromatographic techniques was used for cytotoxic metabolite purification. Chemical structures of the purified metabolites were identified using mass, 1D, and 2D NMR spectroscopic analysis. Results: Bioassay-guided fractionation of the ethyl acetate extract led to the purification of two cytotoxic metabolites, R1 and R2, of reproducible amounts of 5 and 1.5 mg/L, respectively. The structures of R1 and R2 metabolites were identified as β- and γ-rhodomycinone with CD50 of 6.3, 9.45, 64.8 and 9.11, 9.35, 67.3 µg/mL against Caco2, Hela and Vero cell lines, respectively. Values were comparable to those of the positive control doxorubicin. Conclusions: This is the first report about the production of β- and γ-rhodomycinone, two important scaffolds for synthesis of anticancer drugs, from S. griseus.

Notes: Flavonols from Scurrula ferruginea Danser (Loranthaceae)

2002 ◽  
Vol 57 (11-12) ◽  
pp. 1092-1096 ◽  
Author(s):  
Françoise Lohézic-Le Dévéhat ◽  
Sophie Tomasi ◽  
Didier Fontanel ◽  
Joël Boustie
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Ethyl Acetate Fraction ◽  
Flavonol Glycoside ◽  
Spectroscopic Methods ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
Isolated Structures

Three natural flavonols compounds have been isolated from the ethyl acetate fraction of Scurrula ferruginea Danser (Loranthaceae). Besides quercetin and quercitrin, an unusual flavonol glycoside 4″-O-acetylquercitrin was isolated. Structures were determined using spectroscopic methods including UV, NMR and HRMS-EI. The incidence of 4″-O-acetylquercitrin, not previously reported in the Loranthaceae, is discussed. Cytotoxic evaluation on four human cancer cell lines showed quercetin to be the most active with IC50 of 35 μм on U251 (human glioblastoma cells).

scholarly journals Cytotoxic Evaluation of Compounds Isolated from the Aerial Parts of Hedyotis pilulifera and Methanol Extract of Inonotus obliquus

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Hoai Thi Nguyen ◽  
Duc Viet Ho ◽  
Phu Dinh Quynh Nguyen ◽  
Hung Quoc Vo ◽  
Thao Thi Do ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Cancer Cell Lines ◽  
Hep G2 ◽  
Inonotus Obliquus ◽  
Cytotoxic Effects ◽  
First Report ◽  
Aerial Parts

The aim of this study was to evaluate the inhibitory activity of compounds isolated from the aerial parts of Hedyotis pilulifera (Pit.) T.N. Ninh toward selected cancer cell lines. The isolated compounds were identified by analyzing their nuclear magnetic resonance spectral data and physical properties, and comparison of these with reported data. The sulforhodamine B assay was used for the cytotoxic evaluation of isolates. Among twenty-one compounds isolated from H. pilulifera, compounds 2, 3, and 4 showed moderate inhibitory effect on MCF-7 with IC50 values of 63.5, 59.4, and 52.7 μg/mL, respectively, while the other compounds exhibited no effect (IC50 values > 100 μg/mL). Further investigation using HT29, LU-1, HL-60, KB, Hep G2, and SK-Mel2 cancer cell lines showed the moderate cytotoxic activity of compound 3 (IC50 values ranging from 51.7 to 78.3 μg/mL) to all cells, while compound 4 showed selective effect only against HL-60 cells (IC50 61.5 μg/mL). This is the first report of cytotoxic activity of pomolic acid 3β-acetate (3) toward all tested cancer cell lines, and also the first report of cytotoxicity of rotungenic acid (4) against LU-1, HL-60, KB, Hep G2, and SK-Mel2 cancer cell lines. The methanol extract of chaga mushroom { Inonotus obliquus (Ach. ex Pers.) Pilát} exhibited the strongest cytotoxic effects against HL-60 and LU-1 (32.2 and 38.0 μg/mL, respectively), and modest cytotoxic effects against SW480 (41.3 μg/mL), HepG2 (51.3 μg/mL), KB (57.0 μg/mL), and LNCaP (57.7 μg/mL). We conclude that compounds 3 and 4 from H. pilulifera may be useful in further investigation for anticancer agent discovery and chaga could be used as a natural anticancer remedy against promyelocytic leukemia and lung adenocarcinoma.

IN VITRO CYTOTOXIC EVALUATION OF KARHEE WEED

2021 ◽  
Vol 27 (2) ◽  
pp. 119-126
Author(s):  
Shabnam Javed ◽  
Amna Shoaib
Keyword(s):  
Urinary Bladder ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Fraction ◽  
Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Dependent Manner ◽  
Methanolic Extracts

Locally famous Karhee or Berre (Sorbaria tomentosa) exhibits medicinal value is large woody shrub indigenous to Pakistan. The present study examined the cytotoxic activities of S. tomentosa using methanolic extracts and fractions (n-hexane, dichloromethane, ethyl acetate and water) against three cáncer cell lines (lung A-549, hepatocellular HepG2and urinary bladder EI-138). Cytotoxic assays were carried out with five different concentrations (0.05, 0.01, 0.05, 0.1 and 0.5 mg/mL) of methanolic extract and its sub fraction through MTT assay. Results revealed n-hexane and ethyl acetate fraction being the most potent against all test cancer cell lines with higher IC50 values. Both fractions also exhibited the maximum reduction in the cell viability in dose dependent manner. Preliminary results suggest the promising anticancer potential of n-hexane and ethyl acetate S. tomentosa against lung A-549, hepatocellular HepG2and urinary bladder EI-138 cell lines. Further studies are required to know the mechanism(s) involved in the cell death.

scholarly journals Melastoma malabathricum ethyl acetate fraction induces secondary necrosis in human breast and lung cancer cell lines

2017 ◽  
Vol 13 (51) ◽  
pp. 688 ◽  
Author(s):  
ShebaR David ◽  
Adi Idris ◽  
IhsanN Zulkipli ◽  
NurulRamizah Zulhilmi ◽  
HuanF Lee ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Fraction ◽  
Cancer Cell Lines ◽  
Human Breast ◽  
Lung Cancer Cell ◽  
Secondary Necrosis ◽  
Melastoma Malabathricum

scholarly journals Bio-guided Isolation of a New Sesterterpene from Serjania goniocarpa

2015 ◽  
Vol 10 (9) ◽  
pp. 1934578X1501000
Author(s):  
Carlos Quintal-Novelo ◽  
Luis W. Torres-Tapia ◽  
Rosa Moo-Puc ◽  
Sergio R. Peraza-Sanchez
Keyword(s):  
Palmitic Acid ◽  
Traditional Medicine ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Methanol Extract ◽  
Cancer Cell Lines ◽  
Spectroscopic Analyses

Serjania goniocarpa is a plant used in Mayan traditional medicine as a remedy for the treatment of cancer-like symptoms. Bio-guided fractionation of the methanol extract of the leaves led to the isolation of an α- and β-amyrin mixture, palmitic acid, phytol and the new sesterterpene goniocarpic acid whose structure was elucidated by IR, GC-MS, and NMR spectroscopic analyses. Goniocarpic acid exhibited cytotoxic and antiproliferative activity against several cancer cell lines.

scholarly journals Cell Cycle Arrest in Different Cancer Cell Lines (Liver, Breast, and Colon) Induces Apoptosis under the Influence of the Chemical Content of Aeluropus lagopoides Leaf Extracts

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 507
Author(s):  
Kamel Saleh ◽  
Tahani Albinhassan ◽  
Serage Elbehairi ◽  
Mohammed Alshehry ◽  
Mohammad Alfaifi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Chemical Composition ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Flow Cytometer ◽  
Chemical Components ◽  
Aeluropus Lagopoides

Natural products, especially secondary metabolites produced by plants under stressed conditions, are shown to have different pharmacological impacts from one to another. Aeluropus lagopoides is one of the common halophyte plants that survive under stressed conditions, and has been used for healing wounds and as a painkiller. The bioactivity and the chemical composition of this plant have been poorly investigated. Consequently, the chemical components of A. lagopoides leaves were extracted using hexane (nonpolar), ethyl acetate (semi-polar), and n-butanol (polar) to extract the most extensive variety of metabolites. The cytotoxicity and anticancer impact of extracted secondary metabolites were evaluated against breast (MCF-7), colon (HCT-116), and liver (HepG2) cancer cell lines using a SulphoRhodamine-B (SRB) test. Their mechanisms of action were verified by observing the appearance of apoptotic bodies using the fluorescent microscope, while their antiproliferative impacts were evaluated using a flow cytometer. Results revealed that secondary metabolites extracted using hexane and ethyl acetate had the highest cytotoxicity and thus the greatest anticancer activity effect on HepG2 with IC50 (24.29 ± 0.85 and 11.22 ± 0.679 µg/mL, respectively). On the other hand, flow cytometer results showed that secondary metabolites could inhibit the cell cycle in the G0/G1 phase. To ascertain the chemical composition–function relationship, the extracts were analyzed using LC-MS/MS. Accordingly, A. lagopoides hexane and ethyl acetate extracts may contain agents with anticancer potential.

scholarly journals Cytotoxic Activities of Different Iranian Solanaceae and Lamiaceae Plants and Bioassay-Guided Study of an Active Extract from Salvia lachnocalyx

2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Hossein H. Mirzaei ◽  
Omidreza Firuzi ◽  
Ian T. Baldwin ◽  
Amir Reza Jassbi
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Active Species ◽  
Cytotoxic Activities ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
Bioassay Guided Fractionation ◽  
Cytotoxic Compounds

Methanol (MeOH), dichloromethane (DCM) and 80% MeOH extractions of fourteen medicinal plants of the families Solanaceae and Lamiaceae collected from different area of Iran were tested for their cytotoxic potential against MOLT-4 human cancer cell lines. Cytotoxicity of the tested plants indicated that 11 plants had one or two active extracts (IC50 ≤50): MeOH extracts of the shoots of Thymus trautvetteri, Solanum luteum and stems of Lycium shawii; DCM extracts of the shoots of Thymus kotschyanus, Salvia persepolitana, Ballota aucheri, Nepeta glomerulosa, Hyoscyamus tenuicaulis, Salvia lachnocalyx, Salvia sharifii as well as the stems of Salvia verticillata and the roots of Salvia multicaulis and S. lachnocalyx; 80% MeOH extracts of the shoots of T. trautvetteri, S. luteum and the stems of L. shawii. The DCM extract of the aerial parts of S. lachnocalyx as one of the most active species was subjected to the cytotoxic bioassay-guided fractionation and purification using combination of chromatography methods. The bioassay-guided fractionation of DCM extract of the shoots of S. lachnocalyx led to the isolation of two cytotoxic compounds: (2 Z,6 Z,10 Z,14 E)-geranylfarnesol (1), a novel natural product, and spathulenol (2). Both of the isolated compounds, especially 1 (IC50 range: 9.6 −20.2 μg/mL), showed good cytotoxic effects against 3 human cancer cell lines, MOLT-4, MCF-7 and HT-29.

scholarly journals New Ophiobolin Derivatives from the Marine Fungus Aspergillus flocculosus and Their Cytotoxicities against Cancer Cells

Marine Drugs ◽  
2019 ◽  
Vol 17 (6) ◽  
pp. 346 ◽  
Author(s):  
Byeoung-Kyu Choi ◽  
Phan Thi Hoai Trinh ◽  
Hwa-Sun Lee ◽  
Byeong-Woo Choi ◽  
Jong Soon Kang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Chemical Shifts ◽  
Optical Rotation ◽  
2D Nmr ◽  
Cancer Cell Lines ◽  
Marine Fungus ◽  
Side Chain ◽  
Spectroscopic Methods

Five new sesterterpenes, 14,15-dehydro-6-epi-ophiobolin K (1), 14,15-dehydro- ophiobolin K (2), 14,15-dehydro-6-epi-ophiobolin G (3), 14,15-dehydro-ophiobolin G (4) and 14,15-dehydro-(Z)-14-ophiobolin G (5), together with four known ophiobolins (6–9) were isolated from the marine fungus Aspergillus flocculosus derived from the seaweed Padina sp. collected in Vietnam. The five new ophiobolins were first isolated as ophiobolin derivatives consisting of a fully unsaturated side chain. Their structures were elucidated via spectroscopic methods including 1D, 2D NMR and HR-ESIMS. The absolute configurations were determined by the comparison of chemical shifts and optical rotation values with those of known ophiobolins. All compounds (1–9) were then evaluated for their cytotoxicity against six cancer cell lines, HCT-15, NUGC-3, NCI-H23, ACHN, PC-3 and MDA-MB-231. All the compounds showed potent cytotoxicity with GI50 values ranging from 0.14 to 2.01 μM.

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