scholarly journals Cytotoxic activity of casearborin c isolated from Casearia corymbosa

2019 ◽  
Vol 62 (3) ◽  
Author(s):  
María Leonor Vila-Luna ◽  
Rosa Esther Moo-Puc ◽  
Luis Wiliunfo Torres-Tapia ◽  
Sergio Rubén Peraza-Sánchez
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Stem Bark ◽  
Cancer Cell Lines ◽  
Syringic Acid

<p><strong>Abstract. </strong>Casearborin c (<strong>1</strong>), syringic acid (<strong>2</strong>), <em>ent</em>-3β-hydroxy-(-)-13-<em>epi</em>-manoyl oxide (<strong>3</strong>), <em>ent</em>-(-)-13-<em>epi</em>-manoyl oxide (<strong>4</strong>), <em>ent</em>-(-)-kaur-16-en-19-oic acid (<strong>5</strong>), and γ-sitosterol (<strong>6</strong>) were isolated from <em>Casearia corymbosa </em>stem bark. Only casearborin c showed cytotoxic activity on HeLa and SiHa cancer cell lines. This work contributes to the description of three compounds (<strong>1</strong>-<strong>3</strong>) newly isolated from <em>C. corymbosa </em>and highlights that casearborin c, a clerodane-type diterpene, is responsible for the cytotoxic activity shown in the original methanol extract of this species.</p><p> </p><p><strong>Resumen. </strong>Casearborina c (<strong>1</strong>), ácido siríngico (<strong>2</strong>), óxido de <em>ent</em>-3β-hidroxi-(-)-13-<em>epi</em>-manoilo (<strong>3</strong>), óxido de <em>ent</em>-(-)-13-<em>epi</em>-manoilo (<strong>4</strong>), ácido <em>ent</em>-(-)-kaur-16-en-19-oico (<strong>5</strong>) y γ-sitosterol (<strong>6</strong>) se aislaron de la corteza del tallo de <em>Casearia corymbosa</em>. Sólo casearborina c mostró actividad citotóxica en las líneas de células cancerígenas HeLa y SiHa. Este trabajo describe tres compuestos (<strong>1</strong>-<strong>3</strong>) aislados por primera vez de <em>C. corymbosa</em> y destaca que casearborina c, un diterpeno de tipo clerodano, es responsable de la actividad citotóxica del extracto metanólico de esta especie.</p>

scholarly journals Cytotoxic Constituents from the Stem Bark of Alvaradoa amorphoides

2017 ◽  
Vol 59 (3) ◽  
Author(s):  
Sergio R. Peraza-Sanchez
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Chemical Study ◽  
Stem Bark ◽  
Cancer Cell Lines

The chemical study of<strong> </strong><em>Alvaradoa amorphoides</em> led to the isolation of four major constituents: chrysophanol (<strong>1</strong>), β-sitosterol (<strong>2</strong>), atraric acid (<strong>3</strong>), and 17-octadecen-6-ynoic acid (<strong>4</strong>). All compounds were evaluated on <em>in vitro</em> cytotoxic and antiproliferative assays. Atraric acid and 17-octadecen-6-ynoic acid showed cytotoxic activity. Additionally, compound <strong>4</strong> exhibited cytotoxic activity showing a certain degree of selectivity against cancer cell lines.

scholarly journals Cytotoxic Activity of Crude Extracts and Fractions of African Peach (Nauclea latifolia Smith) Stem Bark on Two Cancer Cell Lines

2021 ◽  
pp. 100212
Author(s):  
Oluwakemi Charles-Okhe ◽  
Michael A. Odeniyi ◽  
Titilayo O. Fakeye ◽  
Omonike O. Ogbole ◽  
Toluwanimi E. Akinleye ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Stem Bark ◽  
Cancer Cell Lines ◽  
Crude Extracts ◽  
Nauclea Latifolia

scholarly journals Cytotoxic Evaluation of Compounds Isolated from the Aerial Parts of Hedyotis pilulifera and Methanol Extract of Inonotus obliquus

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Hoai Thi Nguyen ◽  
Duc Viet Ho ◽  
Phu Dinh Quynh Nguyen ◽  
Hung Quoc Vo ◽  
Thao Thi Do ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Cancer Cell Lines ◽  
Hep G2 ◽  
Inonotus Obliquus ◽  
Cytotoxic Effects ◽  
First Report ◽  
Aerial Parts

The aim of this study was to evaluate the inhibitory activity of compounds isolated from the aerial parts of Hedyotis pilulifera (Pit.) T.N. Ninh toward selected cancer cell lines. The isolated compounds were identified by analyzing their nuclear magnetic resonance spectral data and physical properties, and comparison of these with reported data. The sulforhodamine B assay was used for the cytotoxic evaluation of isolates. Among twenty-one compounds isolated from H. pilulifera, compounds 2, 3, and 4 showed moderate inhibitory effect on MCF-7 with IC50 values of 63.5, 59.4, and 52.7 μg/mL, respectively, while the other compounds exhibited no effect (IC50 values > 100 μg/mL). Further investigation using HT29, LU-1, HL-60, KB, Hep G2, and SK-Mel2 cancer cell lines showed the moderate cytotoxic activity of compound 3 (IC50 values ranging from 51.7 to 78.3 μg/mL) to all cells, while compound 4 showed selective effect only against HL-60 cells (IC50 61.5 μg/mL). This is the first report of cytotoxic activity of pomolic acid 3β-acetate (3) toward all tested cancer cell lines, and also the first report of cytotoxicity of rotungenic acid (4) against LU-1, HL-60, KB, Hep G2, and SK-Mel2 cancer cell lines. The methanol extract of chaga mushroom { Inonotus obliquus (Ach. ex Pers.) Pilát} exhibited the strongest cytotoxic effects against HL-60 and LU-1 (32.2 and 38.0 μg/mL, respectively), and modest cytotoxic effects against SW480 (41.3 μg/mL), HepG2 (51.3 μg/mL), KB (57.0 μg/mL), and LNCaP (57.7 μg/mL). We conclude that compounds 3 and 4 from H. pilulifera may be useful in further investigation for anticancer agent discovery and chaga could be used as a natural anticancer remedy against promyelocytic leukemia and lung adenocarcinoma.

scholarly journals Sesquiterpenoids from the Stem Bark of Aglaia simplicifolia and Their Cytotoxic Activity against B16-F10 Melanoma Skin Cancer Cell

2021 ◽  
Vol 21 (6) ◽  
pp. 1560
Author(s):  
Ghina Izdihar ◽  
Al Arofatus Naini ◽  
Desi Harneti ◽  
Rani Maharani ◽  
Nurlelasari Nurlelasari ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cyclopropane Ring ◽  
Stem Bark ◽  
Cancer Cell Lines ◽  
Vitro Assay ◽  
Chemical Structures ◽  
Melanoma Skin

Four sesquiterpenoid derivatives, i.e., 4β,10α-dihydroxyaromadendrane (1), caryophyllenol-II (2), senecrassidiol (3), and clovane-2β,9α-diol (4) have been isolated from the stem bark of Aglaia simplicifolia. The chemical structures of compounds 1-4 were determined based on spectroscopic data, including one and two-dimensional NMR and mass spectroscopy. In addition, these sesquiterpenoids 1-4, were also tested for their cytotoxic activity against B16-F10 melanoma skin cancer cell lines through in vitro assay. Among the isolated compounds 1-4, compound 1 showed the highest activity with an IC50 value of 44.8 μg/mL, suggesting the presence of a cyclopropane ring that plays an essential role in cytotoxic activity against B16-F10 melanoma skin cancer cell lines.

scholarly journals Coronarin K and L: Two Novel Labdane Diterpenes From Roscoea purpurea: An Ayurvedic Crude Drug

2021 ◽  
Vol 9 ◽  
Author(s):  
Venugopal Singamaneni ◽  
Bashir Lone ◽  
Jasvinder Singh ◽  
Pankaj Kumar ◽  
Sumeet Gairola ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Cancer Cell Line ◽  
Cancer Cell Lines ◽  
Lung Cancer Cell Line ◽  
Chloroform Fraction ◽  
Lung Cancer Cell

The main objective of cancer treatment with chemotherapy is to kill the cancerous cells without affecting the healthy normal cells. In the present study, bioactivity-guided purification of the n-chloroform soluble fraction from the methanol extract of Roscoea purpurea resulted in the identification of two new labdane diterpenes: coronarin K (1) and coronarin L (2), along with eight known compounds, coronarin A (3), bisdemethoxycurcumin (4), kaempferol 3-O-methyl ether (5), kaempferol (6), fenozan acid (7), 3-(3-methoxy,4-hydroxyphenyl)-2-propenoic acid ferulic acid (8), caffeic acid (9), and gallic acid (10). The structural identification of new compounds (1 and 2) were determined by detailed analysis of 1D (1H and 13C) and 2D NMR (COSY, HSQC, and HMBC) spectroscopic data. The relative configurations of 1 and 2 were determined with the help of NOESY correlations and comparison of optical rotations with known labdane diterpenes, with established stereochemistry, while structure of known compounds was established by direct comparison of their NMR data with those reported in the literature. This is the first report of isolation of this labdane diterpenes and phenolic classes of secondary metabolites in R. purpurea. In the preliminary screening, the methanol extract and its fractions were tested for the cytotoxic activity against a panel of four cancer cell lines (A549, HCT-116, Bxpc-3, and MCF-7); extract and its chloroform fraction were found to be active against the lung cancer cell line, A-549, with IC50 value &lt;25 μg/ml. Owing to the notable cytotoxic activity of the chloroform fraction, the compounds (1–5) were screened for their cytotoxicity against all the cell lines by MTT assay. Coronarin K, 1 showed significant cytotoxic potential against lung cancer cell lines (A-549), with IC50 value of 13.49 μM, while other compounds did not show activity below 22 μM.

scholarly journals Cytotoxicity assessment of the stem bark of Tieghemella heckelii Pierre ex. A Chev. (Sapotaceae) towards Vero and RD human cancer Cell Lines

2017 ◽  
Vol 6 (1) ◽  
pp. 11-19
Author(s):  
Bertin Kipré Guédé ◽  
◽  
Aya Nathalie Guessennd Kouadio ◽  
Jules N’guessan. Kouadio ◽  
Mamidou Witabouna Koné ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Cell Viability ◽  
Vero Cell ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Cancer Cell Lines ◽  
Vero Cell Lines

The present study aimed at investigating the cytotoxicity of the stem bark of Tieghemella heckelii Pierre ex. A Chev (Sapotaceae). For this purpose, plant extracts were put into contact separately with the different Vero and human RD cancer cell lines diluted in a 96 wells microplate after 24-hour incubation at 37°C. Thereafter, the absorbance was measured every 24 hour for two days, using Elisa reader spectrophotometer. The IC50 values obtained from multi-dose testing of ethanol extract against Vero cell lines ranged from 0.051 to 0.192 mg/mL. The results also showed Vero cell viability of 80.2%, and a mortality rate of 94.9% against RD cell lines whereas methanol extract displayed for the same experiment an IC50 ranging from 0.018 to 2.98 mg/mL with a cell viability of 67%, and a mortality rate of 95.6%. From these results, it could be concluded that the methanol extract of the stem bark showed higher cytotoxic activity towards RD cell lines. As for the ethanol extract, it showed significant non-cytotoxicity towards the Vero cell lines. In the light of this evidence, it can be claimed that the plant exhibited non-cytotoxic patterns against Vero cells and has anticancer potential.

Cytotoxic activity and anti-cancer potential of Ontario grown onion extracts against breast cancer cell lines

2018 ◽  
Vol 8 (3) ◽  
pp. 159 ◽  
Author(s):  
Meghan Fragis ◽  
Abdulmonem I. Murayyan ◽  
Suresh Neethirajan
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Cancer Line ◽  
Mcf 7

Background: Breast cancer is the most commonly diagnosed cancer and the second leading cause of cancer deaths among Canadian women. Cancer management through changes in lifestyle, such as increased intake of foods rich in dietary flavonoids, have been shown to decrease the risk associated with breast, liver, colorectal, and upper-digestive cancers in epidemiologic studies. Onions are high in flavonoid content and one of the most common vegetables. Additionally, onions are used in most Canadian cuisines.Methods: We investigated the effect of five prominent Ontario grown onion (Stanley, Ruby Ring, LaSalle, Fortress, and Safrane) extracts on two subtypes of breast cancer cell lines: a triple negative breast cancer line MDA-MB-231 and an ER+ breast cancer line MCF-7.Results: These onion extracts elicited strong anti-proliferative, anti-migratory, and cytotoxic activities on both the cancer cell lines. Flavonoids present in these onion extracts induced apoptosis, cell cycle arrest in the G2/M phase, and a reduction in mitochondrial membrane potential at dose-dependent concentrations. Onion extracts were more effective against MDA-MB-231 compared to the MCF-7 cell line. Conclusion: In this study, we investigated the extracts synthesized from Ontario-grown onion varieties in inducing anti-migratory, cytostatic, and cytotoxic activities in two sub-types of human breast cancer cell lines. Anti-tumor activity of these extracts depends upon the varietal and can be formulated into nutraceuticals and functional foods for the wellbeing of cancer patients. Overall, the results suggest that onion extracts are a good source of flavonoids with anti-cancerous properties.Keywords: onion extracts; flavonoids; anti-proliferative; breast cancer; cytotoxic activity

scholarly journals Synthesis, Cytotoxic Activity, Crystal Structure, DFT Studies and Molecular Docking of 3-Amino-1-(2,5-dichlorophenyl)-8-methoxy-1H-benzo[f]chromene-2-carbonitrile

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 184
Author(s):  
Menna El Gaafary ◽  
Tatiana Syrovets ◽  
Hany M. Mohamed ◽  
Ahmed A. Elhenawy ◽  
Ahmed M. El-Agrody ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Dna Methyltransferase ◽  
Human Cancer ◽  
Theoretical Calculations ◽  
Cancer Cell Lines ◽  
Molecular Structures ◽  
Cytotoxic Activities ◽  
X Ray

The target compound 3-amino-1-(2,5-d ichlorophenyl)-8-methoxy-1H-benzo[f]-chromene-2-carbonitrile (4) was synthesized via a reaction of 6-methoxynaphthalen-2-ol (1), 2,5-dichlorobenzaldehyde (2), and malononitrile (3) in ethanolic piperidine solution under microwave irradiation. The newly synthesized β-enaminonitrile was characterized by FT-IR, 1H NMR, 13C NMR, mass spectroscopy, elemental analysis and X-ray diffraction data. Its cytotoxic activity was evaluated against three different human cancer cell lines MDA-MB-231, A549, and MIA PaCa-2 in comparison to the positive controls etoposide and camptothecin employing the XTT cell viability assay. The analysis of the Hirshfeld surface was utilized to visualize the reliability of the crystal package. The obtained results confirmed that the tested molecule revealed promising cytotoxic activities against the three cancer cell lines. Furthermore, theoretical calculations (DFT) were carried out with the Becke3-Lee-Yang-parr (B3LYP) level using 6-311++G(d,p) basis. The optimization geometry for molecular structures was in agreement with the X-ray structure data. The HOMO-LUMO energy gap of the studied system was discussed. The intermolecular-interactions were studied through analysis of the topological-electron-density(r) using the QTAIM and NCI methods. The novel compound exhibited favorable ADMET properties and its molecular modeling analysis showed strong interaction with DNA methyltransferase 1.

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